LeinA.00629.a

Acetyl-coenzyme A synthetase, putative

CENTER ID: LeinA.00629.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.00629.a.B1.PW39173 full length 1 695

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LINF_230014800
UniProt: A4I0C2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSKVVSSPV TPNSPLSVSQ HVCDFKRVLD SNVVEPTEAN GKKSHVGPHL GSRMRIYEYS IEHNDAFWAE IARRDFYWKT TWPDDQHVKS YNFDKSKGPI FVKWFEGAVT NVCYNALDRH LPAHKDRVCF YFEGNDPSVT EAVTYGSMYT RVVELANVLK YQYGIAKGDR VGLYLPMIPF AAVAMLACAR IGAVASVIFG GFSAQAIVTR VTDCSPKLII TADASSRGDK PILLKTIADQ ALDDCSVLGC NVPCLVFENM NRQSCKMKEG RDTWYGDALA RLTPEQHEEC PVEWMDAEDV LFLLYTSGST GKPKAIVHTT AGYMVYASTT FMYSFDYHMD DVYFCTADIG WITGHSYVVY GPMIHCATSV LFEGVPNYPD YSRWWQLVEK YKVSILYTAP TAIRSLMQAG DDYVKVGNRS TLRVLGSVGE PINVEAWKWL RDVGGEGHCD VSDTWWQTET GGHMITPMPG CTPMKPGSAT LPFFGVQPVI LDPMKLHEKQ GPAEGLLAIR APWPGMARTI YGDHARFEKT YFGVDGYYMT GDGARRDSDG YYWITGRVDD VLNVSGHRIG TSEIEDAVNT HPAVVESAVV GFPHNIKGEG IYVFLTFRQG TEVTPELLAA VKATVRKVIG PLATPDVLHP AQEGLPKTRS GKIVRRILRK IATHNDDDLG DTSTLADPTV VEALKRSRAK WVDEK
NT Sequence
atgagcagca aagtcgtctc cagtccggtg actccgaaca gcccgctgtc agtctcgcag catgtatgcg acttcaagag ggtgttggac tcgaacgttg tggagccgac agaggcgaac gggaaaaagt cgcacgtcgg cccccacctc ggctctcgca tgcgcatcta cgagtactcc atcgagcaca atgacgcctt ctgggccgaa attgcacggc gcgacttcta ctggaagacg acctggccgg atgaccagca cgtcaagtcg tacaacttcg acaaatccaa gggccccatc tttgtgaagt ggttcgaggg cgccgtgacg aacgtctgct acaacgcgtt ggaccgccac ctgccggcgc acaaggatcg cgtgtgcttt tactttgaag gcaacgaccc gagtgtgacg gaagcagtca cgtacggcag catgtacacg agggtggtgg agctggcgaa cgtgctgaag taccagtacg gtattgcaaa gggcgaccgc gttggcctgt atctacccat gatcccgttc gctgcggtgg ccatgctcgc ctgcgcccgc atcggtgcgg tggccagtgt catctttggc ggattttcgg ctcaggcgat cgtgactcgt gtcacggact gcagcccgaa gctcatcatc actgctgacg cgtcctctcg cggtgataag ccgattctgc tcaagacaat cgcggaccag gccctggacg actgcagcgt ccttgggtgc aacgtcccgt gtctcgtatt cgaaaacatg aaccgtcaat cctgcaagat gaaggaaggc cgagatacct ggtacggcga tgccttagcc cgactgacgc cggagcagca cgaggagtgc ccggtggagt ggatggacgc ggaggatgtg ctgttcctgc tctacacctc cggcagcaca gggaaaccca aagccatcgt gcacacgacg gcgggctaca tggtgtacgc ctccaccacc ttcatgtaca gcttcgacta ccacatggac gacgtgtact tctgcacggc tgatattggc tggatcacag gccacagcta cgtcgtgtat ggtccgatga tccactgcgc cacgtcggtg ctgttcgagg gtgtgccgaa ctaccccgac tactcgcgct ggtggcagct cgtcgagaag tacaaggtgt ccatcctcta caccgcgccc accgcgatcc gctccctcat gcaggccggc gacgactacg tcaaggtggg caaccgcagc accctgcgtg tgctgggctc tgtcggcgag ccgatcaacg tggaggcgtg gaagtggctg cgcgatgtcg gcggcgaggg ccactgcgat gtgtcggaca cgtggtggca gaccgagacg ggcggccaca tgattacacc gatgccgggc tgcaccccca tgaaacccgg tagtgcaacg ctgccgttct tcggtgtcca gccggttatt ctcgacccca tgaagctgca tgagaagcag ggccccgcgg agggcctgct cgccattcgc gcgccgtggc ctggtatggc ccgcaccatc tacggtgacc acgcacgctt tgagaaaacc tactttggcg tggacgggta ctacatgaca ggcgacggtg ctcgccgcga ctcggacggc tattactgga tcacgggccg cgtcgatgac gtgctgaacg tgagcgggca ccgcatcggc accagcgaga tcgaagacgc cgtcaacacc cacccggccg tggtggagag cgccgtcgtc ggcttccctc acaacatcaa gggtgagggc atctacgtct tccttacgtt ccgccagggg acggaggtga cgcccgagct gctggcggcg gtgaaggcga ctgtgcgcaa ggtgattggc ccgctggcca cgccggacgt gcttcacccc gcgcaggaag gactgcccaa aacgcgctcc ggcaagattg tgcgccgcat cctgcgcaag atcgccacgc acaacgacga cgacctgggc gacacctcga ccctggcaga ccccaccgtc gtggaggcgc tgaagcgctc gcgtgcgaag tgggtggatg aaaagtag
Details for LeinA.00629.a.B1.PW39173
PURIFICATION DATe: 12/19/2022
CONCENTRATION: 19.98mg/ml
OBSERVED MW: 78kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL21 DE3
VIAL COUNT (approx.): 6
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS SKVVSSPVTP NSPLSVSQHV CDFKRVLDSN VVEPTEANGK KSHVGPHLGS RMRIYEYSIE HNDAFWAEIA RRDFYWKTTW PDDQHVKSYN FDKSKGPIFV KWFEGAVTNV CYNALDRHLP AHKDRVCFYF EGNDPSVTEA VTYGSMYTRV VELANVLKYQ YGIAKGDRVG LYLPMIPFAA VAMLACARIG AVASVIFGGF SAQAIVTRVT DCSPKLIITA DASSRGDKPI LLKTIADQAL DDCSVLGCNV PCLVFENMNR QSCKMKEGRD TWYGDALARL TPEQHEECPV EWMDAEDVLF LLYTSGSTGK PKAIVHTTAG YMVYASTTFM YSFDYHMDDV YFCTADIGWI TGHSYVVYGP MIHCATSVLF EGVPNYPDYS RWWQLVEKYK VSILYTAPTA IRSLMQAGDD YVKVGNRSTL RVLGSVGEPI NVEAWKWLRD VGGEGHCDVS DTWWQTETGG HMITPMPGCT PMKPGSATLP FFGVQPVILD PMKLHEKQGP AEGLLAIRAP WPGMARTIYG DHARFEKTYF GVDGYYMTGD GARRDSDGYY WITGRVDDVL NVSGHRIGTS EIEDAVNTHP AVVESAVVGF PHNIKGEGIY VFLTFRQGTE VTPELLAAVK ATVRKVIGPL ATPDVLHPAQ EGLPKTRSGK IVRRILRKIA THNDDDLGDT STLADPTVVE ALKRSRAKWV DEK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA GCAAAGTCGT CTCCAGTCCG GTGACTCCGA ACAGCCCGCT GTCAGTCTCG CAGCATGTAT GCGACTTCAA GAGGGTGTTG GACTCGAACG TTGTGGAGCC GACAGAGGCG AACGGGAAAA AGTCGCACGT CGGCCCCCAC CTCGGCTCTC GCATGCGCAT CTACGAGTAC TCCATCGAGC ACAATGACGC CTTCTGGGCC GAAATTGCAC GGCGCGACTT CTACTGGAAG ACGACCTGGC CGGATGACCA GCACGTCAAG TCGTACAACT TCGACAAATC CAAGGGCCCC ATCTTTGTGA AGTGGTTCGA GGGCGCCGTG ACGAACGTCT GCTACAACGC GTTGGACCGC CACCTGCCGG CGCACAAGGA TCGCGTGTGC TTTTACTTTG AAGGCAACGA CCCGAGTGTG ACGGAAGCAG TCACGTACGG CAGCATGTAC ACGAGGGTGG TGGAGCTGGC GAACGTGCTG AAGTACCAGT ACGGTATTGC AAAGGGCGAC CGCGTTGGCC TGTATCTACC CATGATCCCG TTCGCTGCGG TGGCCATGCT CGCCTGCGCC CGCATCGGTG CGGTGGCCAG TGTCATCTTT GGCGGATTTT CGGCTCAGGC GATCGTGACT CGTGTCACGG ACTGCAGCCC GAAGCTCATC ATCACTGCTG ACGCGTCCTC TCGCGGTGAT AAGCCGATTC TGCTCAAGAC AATCGCGGAC CAGGCCCTGG ACGACTGCAG CGTCCTTGGG TGCAACGTCC CGTGTCTCGT ATTCGAAAAC ATGAACCGTC AATCCTGCAA GATGAAGGAA GGCCGAGATA CCTGGTACGG CGATGCCTTA GCCCGACTGA CGCCGGAGCA GCACGAGGAG TGCCCGGTGG AGTGGATGGA CGCGGAGGAT GTGCTGTTCC TGCTCTACAC CTCCGGCAGC ACAGGGAAAC CCAAAGCCAT CGTGCACACG ACGGCGGGCT ACATGGTGTA CGCCTCCACC ACCTTCATGT ACAGCTTCGA CTACCACATG GACGACGTGT ACTTCTGCAC GGCTGATATT GGCTGGATCA CAGGCCACAG CTACGTCGTG TATGGTCCGA TGATCCACTG CGCCACGTCG GTGCTGTTCG AGGGTGTGCC GAACTACCCC GACTACTCGC GCTGGTGGCA GCTCGTCGAG AAGTACAAGG TGTCCATCCT CTACACCGCG CCCACCGCGA TCCGCTCCCT CATGCAGGCC GGCGACGACT ACGTCAAGGT GGGCAACCGC AGCACCCTGC GTGTGCTGGG CTCTGTCGGC GAGCCGATCA ACGTGGAGGC GTGGAAGTGG CTGCGCGATG TCGGCGGCGA GGGCCACTGC GATGTGTCGG ACACGTGGTG GCAGACCGAG ACGGGCGGCC ACATGATTAC ACCGATGCCG GGCTGCACCC CCATGAAACC CGGTAGTGCA ACGCTGCCGT TCTTCGGTGT CCAGCCGGTT ATTCTCGACC CCATGAAGCT GCATGAGAAG CAGGGCCCCG CGGAGGGCCT GCTCGCCATT CGCGCGCCGT GGCCTGGTAT GGCCCGCACC ATCTACGGTG ACCACGCACG CTTTGAGAAA ACCTACTTTG GCGTGGACGG GTACTACATG ACAGGCGACG GTGCTCGCCG CGACTCGGAC GGCTATTACT GGATCACGGG CCGCGTCGAT GACGTGCTGA ACGTGAGCGG GCACCGCATC GGCACCAGCG AGATCGAAGA CGCCGTCAAC ACCCACCCGG CCGTGGTGGA GAGCGCCGTC GTCGGCTTCC CTCACAACAT CAAGGGTGAG GGCATCTACG TCTTCCTTAC GTTCCGCCAG GGGACGGAGG TGACGCCCGA GCTGCTGGCG GCGGTGAAGG CGACTGTGCG CAAGGTGATT GGCCCGCTGG CCACGCCGGA CGTGCTTCAC CCCGCGCAGG AAGGACTGCC CAAAACGCGC TCCGGCAAGA TTGTGCGCCG CATCCTGCGC AAGATCGCCA CGCACAACGA CGACGACCTG GGCGACACCT CGACCCTGGC AGACCCCACC GTCGTGGAGG CGCTGAAGCG CTCGCGTGCG AAGTGGGTGG ATGAAAAGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT