LeinA.00600.a

tryptophanyl-tRNA synthetase, putative

CENTER ID: LeinA.00600.a
ORGANISM: Leishmania infantum
ASSOCIATED DISEASE: Visceral leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LeinA.00600.a.B1.GE42524 full length 1 412
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LinJ.23.0340
UniProt: A4I070

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDDGARQHMH DVMAKRRKRA MTTTPSANAE GVATPDLTVV PSGDATPQAA ASPDMQQRQQ PPALALHHFF NRDIAFSHRD LHKALVDIEA SIKTGDCSVF LYTGRGPSTG TMHLGHVLPF MLTKYLQDVF NLPLVIQITD DEKFLFRDVP FEGAKADELI RSNIKDIIAF DFNPRHTFIF RNTHYMGDMY PTVLRLQRCM TGNAVKHTLG ITDSDNVGKL AFPATQAAPC FSTAFRRVLQ NGDRPMRCLI PCAIDQDPFF VLTRAAALRL KQLPPALLHT KFLPALKGLE HKMSSSAEEK GVITLHDTDK QVRKKLRRAF SGGCATLEQM QEAGANLEVD VAYQYLRFFC PDDTLFADVT QRYRSGALNS GEVKDLAADC IIREVLHDWR KRRATVTDDD VVEFCSIRNI LL
NT Sequence
atggacgacg gtgcgcgtca gcacatgcac gatgtcatgg ccaagcgccg caagcgggcc atgaccacca ccccatccgc taacgccgag ggcgtcgcga ccccggattt gactgtggtg ccatctggag atgcgacacc gcaggccgcc gcgtcccctg acatgcagca gcggcagcag ccaccagcac tggcgcttca ccacttcttc aaccgcgaca ttgccttctc gcaccgcgat ttgcacaagg cgcttgtgga tatcgaggct tccatcaaaa cgggagactg ctctgtcttc ctctacactg gccgtggccc tagcactggc accatgcact tgggccatgt gctgcccttc atgctcacca agtacctcca agacgtcttc aaccttccgc tcgttataca aatcacagat gacgaaaagt ttctctttcg cgacgtcccc ttcgagggcg ccaaggcgga cgagcttatt cgcagcaaca tcaaggatat catcgccttc gacttcaacc cgcgccacac cttcatattc cgaaacacgc actacatggg cgatatgtac ccgacggtgc tccggctgca gcgttgcatg acaggcaacg ctgtgaagca cacgctcggc atcacggaca gcgataatgt cggcaaactc gcctttcctg ccacccaggc tgctccgtgc ttcagcaccg ctttccgtcg tgttctgcag aacggcgaca ggccgatgcg gtgcctgatt ccctgcgcca ttgatcagga tcccttcttc gttctcacgc gcgccgccgc gctgcggctg aagcagctac caccggccct gcttcacacg aagttcctgc cggctctcaa aggcctggag cacaagatga gcagcagcgc agaggagaag ggcgtcatta ccctacatga cacagataaa caggtgcgca agaagctgcg ccgcgccttc tcgggcggct gcgccaccct agagcagatg caagaggcgg gcgccaacct ggaggtggat gtggcgtacc agtacctgcg gttcttctgc cctgatgaca cactcttcgc cgacgtgacg cagcgctacc gcagcggtgc cctgaatagc ggagaggtga aagacctggc ggcggattgc atcatccgtg aggtgctgca cgattggcgg aagcggcgcg caacggtgac ggacgatgac gtggtggagt tctgcagtat ccgtaacatt cttctctga
Details for LeinA.00600.a.B1.GE42524
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 47kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMD DGARQHMHDV MAKRRKRAMA TTPSANAEGV ATPDLTVVPS GDATPQAAAS PDMQQRQQPP ALALHHFFNR DIAFSHRDLH KALVDIEASI KTGECSVFLY TGRGPSTGAM HLGHVLPFML TKYLQDVFNL PLVIQITDDE KFLFRDVPFE GAKADELIRS NIKDIIAFDF NPRHTFIFRN THYMGDMYPT VLRLQRCMTG NAVKHTLGIT DSDNVGKLAF PATQAAPCFS TAFRRVLQNG DRPMRCLIPC AIDQDPFFVL TRAAALRLKQ LPPALLHTKF LPALKGLEHK MSSSAEEKGV ITLHDTDKQV RKKLRRAFSG GCATLEQMQE AGAILEVDVA YQYLRFFCPD DTLFADVTQR YRSGALNSGE VKDLAADCII REVLHDWRKR RATVTDDDVV E
Validated NT Sequence
accatggctc accaccacca ccaccatatg gacgacggtg cgcgtcagca catgcacgat gtcatggcca agcgccgcaa gcgggccatg gccaccaccc catccgctaa cgctgagggc gtcgcgaccc cggatttgac tgtggtgcca tctggagatg cgacaccgca ggccgccgcg tcccctgaca tgcagcagcg gcagcagcca ccagcactgg cgcttcacca cttcttcaac cgcgacattg ccttctcgca ccgcgatttg cacaaggcgc ttgtggatat cgaggcttcc atcaaaacgg gagagtgctc tgtcttcctc tacactggcc gtggccctag cactggcgcc atgcacttgg gccatgtgct gcccttcatg ctcaccaagt acctccaaga cgtcttcaac cttccgctcg ttatacaaat cacagatgac gaaaagtttc tctttcgcga cgtccccttc gagggcgcca aggcggacga gcttattcgc agcaacatca aggatatcat cgccttcgac ttcaacccgc gccacacctt catattccga aacacgcact acatgggcga tatgtacccg acggtgctcc ggctgcagcg ttgcatgaca ggcaacgctg tgaagcacac gctcggcatc acggacagcg ataatgtcgg caaactcgcc tttcctgcca cccaggctgc tccgtgcttc agcaccgctt tccgtcgtgt tctgcagaac ggcgacaggc cgatgcggtg cctgattccc tgcgccattg atcaggatcc cttcttcgtt ctcacgcgcg ccgccgcgct gcggctgaag cagctaccac cggccctgct tcacacgaag ttcctgccgg ctctcaaagg cctggagcac aagatgagca gcagcgcaga ggagaagggc gtcattaccc tacatgacac agataaacag gtgcgcaaga agctgcgccg cgccttctcg ggcggctgcg ccaccctaga gcagatgcaa gaggcgggcg ccatcctgga ggtggatgtg gcgtaccagt acctgcggtt cttctgccct gatgacacac tcttcgccga cgtgacgcag cgctaccgca gcggtgccct gaatagcgga gaggtgaaag acctggcggc ggattgcatc atccgtgagg tgctgcacga ttggcggaag cggcgcgcaa cggtgacgga cgatgacgtg gtggagttc
Expected Protein Sequence
MAHHHHHHMD DGARQHMHDV MAKRRKRAMT TTPSANAEGV ATPDLTVVPS GDATPQAAAS PDMQQRQQPP ALALHHFFNR DIAFSHRDLH KALVDIEASI KTGDCSVFLY TGRGPSTGTM HLGHVLPFML TKYLQDVFNL PLVIQITDDE KFLFRDVPFE GAKADELIRS NIKDIIAFDF NPRHTFIFRN THYMGDMYPT VLRLQRCMTG NAVKHTLGIT DSDNVGKLAF PATQAAPCFS TAFRRVLQNG DRPMRCLIPC AIDQDPFFVL TRAAALRLKQ LPPALLHTKF LPALKGLEHK MSSSAEEKGV ITLHDTDKQV RKKLRRAFSG GCATLEQMQE AGANLEVDVA YQYLRFFCPD DTLFADVTQR YRSGALNSGE VKDLAADCII REVLHDWRKR RATVTDDDVV EFCSIRNILL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacg acggtgcgcg tcagcacatg cacgatgtca tggccaagcg ccgcaagcgg gccatgacca ccaccccatc cgctaacgcc gagggcgtcg cgaccccgga tttgactgtg gtgccatctg gagatgcgac accgcaggcc gccgcgtccc ctgacatgca gcagcggcag cagccaccag cactggcgct tcaccacttc ttcaaccgcg acattgcctt ctcgcaccgc gatttgcaca aggcgcttgt ggatatcgag gcttccatca aaacgggaga ctgctctgtc ttcctctaca ctggccgtgg ccctagcact ggcaccatgc acttgggcca tgtgctgccc ttcatgctca ccaagtacct ccaagacgtc ttcaaccttc cgctcgttat acaaatcaca gatgacgaaa agtttctctt tcgcgacgtc cccttcgagg gcgccaaggc ggacgagctt attcgcagca acatcaagga tatcatcgcc ttcgacttca acccgcgcca caccttcata ttccgaaaca cgcactacat gggcgatatg tacccgacgg tgctccggct gcagcgttgc atgacaggca acgctgtgaa gcacacgctc ggcatcacgg acagcgataa tgtcggcaaa ctcgcctttc ctgccaccca ggctgctccg tgcttcagca ccgctttccg tcgtgttctg cagaacggcg acaggccgat gcggtgcctg attccctgcg ccattgatca ggatcccttc ttcgttctca cgcgcgccgc cgcgctgcgg ctgaagcagc taccaccggc cctgcttcac acgaagttcc tgccggctct caaaggcctg gagcacaaga tgagcagcag cgcagaggag aagggcgtca ttaccctaca tgacacagat aaacaggtgc gcaagaagct gcgccgcgcc ttctcgggcg gctgcgccac cctagagcag atgcaagagg cgggcgccaa cctggaggtg gatgtggcgt accagtacct gcggttcttc tgccctgatg acacactctt cgccgacgtg acgcagcgct accgcagcgg tgccctgaat agcggagagg tgaaagacct ggcggcggat tgcatcatcc gtgaggtgct gcacgattgg cggaagcggc gcgcaacggt gacggacgat gacgtggtgg agttctgcag tatccgtaac attcttctct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat