SSGCID: Target Page

LedoB.20384.a

Protein kinase, putative

CENTER ID:	LedoB.20384.a
ORGANISM:	Leishmania donovani BPK282A1
ASSOCIATED DISEASE:	Leishmaniasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LedoB.20384.a.HZ2.GE43610	cSMRT Kinase domain		1	418
LedoB.20384.a.HZ3.GE43715	kinase domain (Jensen)		1	511

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LedoB.20384.a.HZ2.PW38871	cSMRT Kinase domain		1	418

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LdBPK_210330.1
UniProt:	E9BF18

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MATVDTVRGR YSSYKLLNEV GRGGSALVYR AQDVATRKDV AVKQLFGNRP QDMEDWLREV DALHTLNNHH CPHVVKYLDH MQQHDRLFLV EEFTEHGSLL RRLKENSKLP EDIACRYIYQ VLTALYHMAP WGVVHGDLKA SNILLFDGDV VKLTDFALRS HGEDDHEDGE RRTTAASTPS AEALPLMRGS RAGRGAGEEC SGSALSVFRG SAYWAAPEVL AGASKATAAS DIWSVGCLAV ELLTGAPPYF ERPIHNAIHH ILKSYYEVLC ESEASRVGAA GTSRASTNPA TPSAAMGSGD DDGPRGPRKK KNGAKDGCGA APQKTDVASA RRATATTSTH NPPKEPVDAV KTAASSGAVC ASDRNEVATP LCEAALLPPL PEDVHLSDEC LSFLRMCFRP RAVDRPSAGE LLHDPWFLDC TVPQLLRAAR EGRPMNGTTG DESVSAGTSS GSRFGVIEQW VKRNLTCDNE SRCEAWLNSD ALPLLVPVLT PRIMTPKYIG NVMWCFSQFA ESRSALATLF VDRLGSTELW GVEELTSACD ADHLATLFRR CCATQDAQVP VYTPADPRAL RFVLGLEKEK VLACVRALHS RLVLEPTAAA MAADASSLAT ATDDTAVDLN DCPASSRDPA APAHAAPDRA PASLQEQHEQ QQRARERLLS DGGAAVLCQC VEAQCKAAFL SNTAPMMEWS TMNLLFDVLC TIEPLAGGQA LLWGLGADLS GGHTSPSSTV GTGGGGAAIL KSLTVVVNPC GGRGGGELNS TWEKSSPGTG PPLLVSPISV SPQESIPTTA AAAGGGGGPL SSLGSTMGFG CHTVSGLPPE SVQWATSMTW LLAVQEAARH LCEAAVRLLT RYIPVARRCR AEYLEKAGVS LTATLVLVAS SEVVSTDVRC AAVEALPQLQ ASSLRATRYL RDPVRCIALL ALTLKRSYGV PALTSCLLTA ITAMTSEKQM LAACTGCPWV WESLVSLLRE VEAADKAAAA GGKRSSSALH SAASTDGGAA EGAVAPPALV AAAASPTSVK SPSASSSSGA VAAGGAAVFA DIVVLLSRWF AEVTPTALLS SAVTAAAAAS EAIPALGHPA ASLPLPVFLQ TLRCQLIALS QNGRVCHGDL MPDVAKALRH LAPLEAAAVA ASTSTATVA

NT Sequence

atggccaccg tagacaccgt cagggggcgg tactcgtcct acaagctgct caacgaagtt ggccgcggtg gcagcgcgct cgtgtaccgc gcgcaggacg tggccacacg caaagatgtt gccgtgaagc agctctttgg caaccggccg caggacatgg aagactggct gcgcgaggtg gacgccctac acacgctcaa caaccatcat tgcccgcatg tcgtgaagta cctggaccac atgcagcagc acgatcgact cttcctcgtg gaggagttca ccgagcacgg atcgctgctg cgccgtctga aggaaaacag caagcttccg gaggacatcg cttgccggta tatctaccag gtgctgacgg ccctgtatca tatggccccg tggggcgtcg tgcacggcga cttgaaggcg tcaaacattc ttctcttcga cggcgatgtc gtgaaactga cggactttgc actgcgctcg catggcgagg acgatcacga ggatggcgag cggcgcacca ccgcggcctc cacgccttct gccgaagcac tgcccctcat gaggggcagc cgcgccggga ggggcgctgg tgaagagtgc tctggctctg ccctctcggt gttccgcggc tccgcttact gggcagcgcc ggaagtcctc gccggtgcat cgaaggcaac ggcggcgagc gacatctggt cggtgggctg cctcgcggtg gagttgctca cgggtgcccc gccgtacttc gagcgcccca tccacaacgc cattcatcac atcctcaaga gctactacga ggtgctctgt gaaagcgagg ccagccgcgt cggtgctgcc ggcacctcgc gcgcgtccac caaccccgca accccaagcg cggcaatggg ctctggcgac gatgacgggc cgcgtgggcc ccggaagaag aaaaacggag cgaaagacgg gtgtggtgca gcgccgcaaa aaacagacgt ggcgtccgcg cggagggcga cggcaacgac gagcacccac aatccgccga aggagccggt cgatgcggtc aagacagccg catctagcgg cgctgtgtgt gcatcagacc ggaatgaagt ggccacgcct ctgtgcgagg cggcgctgct cccgccgctt cccgaggacg tgcacttgag cgatgaatgt ctgtcgtttc tacggatgtg cttccgcccc cgcgcggtag accggccgtc ggccggagag ctgctccacg atccgtggtt tctggactgc accgtgccgc agcttctgcg ggcagcgcgc gagggtcggc ctatgaacgg gacgacaggc gacgagagcg tcagcgccgg caccagcagc ggcagccgct ttggggtgat tgagcagtgg gtgaagcgca acctgacatg tgataacgag agccggtgcg aggcgtggct gaacagcgac gcactgccgc tgctggtgcc ggtgctgacg ccgcgtatta tgacacccaa gtacattggg aacgtgatgt ggtgcttttc ccagtttgcc gagagccgct cagccttggc gacgctcttt gtggaccggt tggggtcgac agagctctgg ggggtggagg agctgacgtc ggcgtgcgac gcggaccacc tcgccaccct ctttcggcgg tgctgtgcaa cgcaggacgc acaggtgccc gtgtacaccc cggcagaccc gcgtgcgctg cgctttgtcc tgggcctgga gaaggaaaag gttctggcat gtgtgcgtgc gctgcactcg cgtctcgtgc tcgagccgac ggccgcggcg atggctgcgg atgcgagctc gctggccacc gccacagacg acaccgcagt ggatctgaac gactgccccg cctcctcgcg tgaccccgcg gcgcccgcgc atgcggcgcc ggatcgcgca ccggcatccc ttcaagagca gcatgaacaa cagcagcgtg cccgtgagcg actactcagc gacggcggcg cggcggtgct gtgccagtgc gtggaggcgc agtgcaaggc agctttcctg agcaacacag cgcccatgat ggagtggagc acgatgaacc tgctgtttga cgtgctctgc accatcgagc cgctggcggg cgggcaggcg ttgctgtggg ggctcggcgc tgacctcagc ggtggtcaca cctccccgag ctctactgtg ggcacaggtg gtggtggagc ggcgattttg aaatcgctga cggttgtcgt caacccgtgc ggaggacgag gcggcggtga gttgaacagc acctgggaaa agtcgtcgcc agggacaggt cccccgcttt tggtgtcacc gatctcggtg agcccgcagg agtcgattcc gacgacagcg gcagcggcgg gcggcggtgg cggcccgctg agctccctag gcagcactat ggggttcgga tgccacaccg tcagcggcct tccaccggag tcagtgcagt gggccacatc tatgacatgg ctgctcgcag tgcaggaagc agcgcgccac ctgtgcgagg cagcggtgcg cctgctcacc cgctacatcc cagtcgccag aaggtgcagg gccgagtacc tcgagaaggc cggtgtcagt ctgactgcaa cgctggtcct cgtggcctcg agcgaggtgg tcagcaccga cgtacgatgc gcggctgtcg aagccctgcc gcagctgcag gcgagctcgc tccgtgcaac ccggtacctg cgcgacccgg tgcgctgcat tgccctgctg gcgctcaccc tgaagcggtc ctacggtgtc cccgcgctga catcgtgcct gctcacagcc atcaccgcta tgacgtctga gaagcagatg ctagcggcct gcaccggctg cccgtgggtg tgggaatcac tggtgtccct gctaagagaa gtggaggcag ctgacaaagc cgccgccgct ggtggcaagc gcagcagctc ggctctccac agcgccgcca gcaccgacgg tggcgcggcc gagggggccg tcgccccacc tgccttagta gcagcagcgg cgagtcccac atcagtcaaa tcgcctagcg ccagcagcag cagcggcgct gttgctgctg gtggcgctgc tgtttttgcc gacattgttg ttcttctctc gcgttggttc gcggaggtga caccgacggc gctgctgagc agtgccgtaa cagcagcagc agcggcatcg gaagcaatac ctgccctcgg tcatccagca gcgtccctac ccctcccggt gtttctgcag acacttcgct gccagctcat agcgctgtcg cagaacggtc gtgtctgcca cggtgatctg atgccggatg tggcgaaggc gctgcggcat ttggcccctc tcgaagccgc cgccgtcgcc gcgtcaacgt cgacggccac ggtggcgtga

Details for LedoB.20384.a.HZ2.GE43610

HARVESTED ON:	2/3/2020
SEQUENCED ON:	2/13/2020
EXPECTED MW:	59kDa
OBSERVED MW:	59kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	74
PERCENT COVERAGE:	37

Validated AA Sequence

MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMA XXDTVRGRYX XLQAAQRSWP RWQRARVPRA XXGXXXXCCX XAXLWQPAAX HGRLAARXXR PTHAQXPSLP ACREXTWTT

Validated NT Sequence

atggctcatc accatcacca tcatatgaag tcggactcag aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg ataacgatat tattgaggct cacagagaac agattggtgg taccctggaa gctcagaccc agggtcctgg ttcgatggcn nccgnagaca ccgtcagggg gcggtactnn nncctacaag ctgctcaacg aagttggccg cggtggcagc gcgctcgtgt accgcgcgca nnnnnnggcc ncnnncnnng atgttgccnn gnngcanctc tttggcaacc ggccgcanga catggaagac tggctgcgcg angngnacgc cctacacacg ctcaacnncc atcattgccc gcatgtcgng aangtacctg gaccacntgc

Expected Protein Sequence

MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMA TVDTVRGRYS SYKLLNEVGR GGSALVYRAQ DVATRKDVAV KQLFGNRPQD MEDWLREVDA LHTLNNHHCP HVVKYLDHMQ QHDRLFLVEE FTEHGSLLRR LKENSKLPED IACRYIYQVL TALYHMAPWG VVHGDLKASN ILLFDGDVVK LTDFALRSHG EDDHEDGERR TTAASTPSAE ALPLMRGSRA GRGAGEECSG SALSVFRGSA YWAAPEVLAG ASKATAASDI WSVGCLAVEL LTGAPPYFER PIHNAIHHIL KSYYEVLCES EASRVGAAGT SRASTNPATP SAAMGSGDDD GPRGPRKKKN GAKDGCGAAP QKTDVASARR ATATTSTHNP PKEPVDAVKT AASSGAVCAS DRNEVATPLC EAALLPPLPE DVHLSDECLS FLRMCFRPRA VDRPSAGELL HDPWFL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tggccaccgt agacaccgtc agggggcggt actcgtccta caagctgctc aacgaagttg gccgcggtgg cagcgcgctc gtgtaccgcg cgcaggacgt ggccacacgc aaagatgttg ccgtgaagca gctctttggc aaccggccgc aggacatgga agactggctg cgcgaggtgg acgccctaca cacgctcaac aaccatcatt gcccgcatgt cgtgaagtac ctggaccaca tgcagcagca cgatcgactc ttcctcgtgg aggagttcac cgagcacgga tcgctgctgc gccgtctgaa ggaaaacagc aagcttccgg aggacatcgc ttgccggtat atctaccagg tgctgacggc cctgtatcat atggccccgt ggggcgtcgt gcacggcgac ttgaaggcgt caaacattct tctcttcgac ggcgatgtcg tgaaactgac ggactttgca ctgcgctcgc atggcgagga cgatcacgag gatggcgagc ggcgcaccac cgcggcctcc acgccttctg ccgaagcact gcccctcatg aggggcagcc gcgccgggag gggcgctggt gaagagtgct ctggctctgc cctctcggtg ttccgcggct ccgcttactg ggcagcgccg gaagtcctcg ccggtgcatc gaaggcaacg gcggcgagcg acatctggtc ggtgggctgc ctcgcggtgg agttgctcac gggtgccccg ccgtacttcg agcgccccat ccacaacgcc attcatcaca tcctcaagag ctactacgag gtgctctgtg aaagcgaggc cagccgcgtc ggtgctgccg gcacctcgcg cgcgtccacc aaccccgcaa ccccaagcgc ggcaatgggc tctggcgacg atgacgggcc gcgtgggccc cggaagaaga aaaacggagc gaaagacggg tgtggtgcag cgccgcaaaa aacagacgtg gcgtccgcgc ggagggcgac ggcaacgacg agcacccaca atccgccgaa ggagccggtc gatgcggtca agacagccgc atctagcggc gctgtgtgtg catcagaccg gaatgaagtg gccacgcctc tgtgcgaggc ggcgctgctc ccgccgcttc ccgaggacgt gcacttgagc gatgaatgtc tgtcgtttct acggatgtgc ttccgccccc gcgcggtaga ccggccgtcg gccggagagc tgctccacga tccgtggttt ctgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for LedoB.20384.a.HZ3.GE43715

HARVESTED ON:	data unavailable
SEQUENCED ON:	6/23/2020
EXPECTED MW:	69kDa
OBSERVED MW:	69kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tggccaccgt agacaccgtc agggggcggt actcgtccta caagctgctc aacgaagttg gccgcggtgg cagcgcgctc gtgtaccgcg cgcaggacgt ggccacacgc aaagatgttg ccgtgaagca gctctttggc aaccggccgc aggacatgga agactggctg cgcgaggtgg acgccctaca cacgctcaac aaccatcatt gcccgcatgt cgtgaagtac ctggaccaca tgcagcagca cgatcgactc ttcctcgtgg aggagttcac cgagcacgga tcgctgctgc gccgtctgaa ggaaaacagc aagcttccgg aggacatcgc ttgccggtat atctaccagg tgctgacggc cctgtatcat atggccccgt ggggcgtcgt gcacggcgac ttgaaggcgt caaacattct tctcttcgac ggcgatgtcg tgaaactgac ggactttgca ctgcgctcgc atggcgagga cgatcacgag gatggcgagc ggcgcaccac cgcggcctcc acgccttctg ccgaagcact gcccctcatg aggggcagcc gcgccgggag gggcgctggt gaagagtgct ctggctctgc cctctcggtg ttccgcggct ccgcttactg ggcagcgccg gaagtcctcg ccggtgcatc gaaggcaacg gcggcgagcg acatctggtc ggtgggctgc ctcgcggtgg agttgctcac gggtgccccg ccgtacttcg agcgccccat ccacaacgcc attcatcaca tcctcaagag ctactacgag gtgctctgtg aaagcgaggc cagccgcgtc ggtgctgccg gcacctcgcg cgcgtccacc aaccccgcaa ccccaagcgc ggcaatgggc tctggcgacg atgacgggcc gcgtgggccc cggaagaaga aaaacggagc gaaagacggg tgtggtgcag cgccgcaaaa aacagacgtg gcgtccgcgc ggagggcgac ggcaacgacg agcacccaca atccgccgaa ggagccggtc gatgcggtca agacagccgc atctagcggc gctgtgtgtg catcagaccg gaatgaagtg gccacgcctc tgtgcgaggc ggcgctgctc ccgccgcttc ccgaggacgt gcacttgagc gatgaatgtc tgtcgtttct acggatgtgc ttccgccccc gcgcggtaga ccggccgtcg gccggagagc tgctccacga tccgtggttt ctggactgca ccgtgccgca gcttctgcgg gcagcgcgcg agggtcggcc tatgaacggg acgacaggcg acgagagcgt cagcgccggc accagcagcg gcagccgctt tggggtgatt gagcagtggg tgaagcgcaa cctgacatgt gataacgaga gccggtgcga ggcgtggctg aacagcgacg cactgccgct gctggtgccg gtgctgacgc cgcgtattat gacacccaag tacattggga acgtgatgtg gtgcttttcc cagtttgccg agtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for LedoB.20384.a.HZ2.PW38871

PURIFICATION DATe:	7/14/2020
CONCENTRATION:	13mg/ml
OBSERVED MW:	60kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	8
VIAL VOLUME:	110µl
PERCENT IDENTITY:	74
PERCENT COVERAGE:	37

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGAAGTCGGA CTCAGAAGTC AATCAAGAAG CTAAGCCAGA GGTCAAGCCA GAAGTCAAGC CTGAGACTCA CATCAATTTA AAGGTGTCCG ATGGATCTTC AGAGATCTTC TTCAAGATCA AAAAGACCAC TCCTTTAAGA AGGCTGATGG AAGCGTTCGC TAAAAGACAG GGTAAGGAAA TGGACTCCTT AAGATTCTTG TACGACGGTA TTAGAATTCA AGCTGATCAG ACCCCTGAAG ATTTGGACAT GGAGGATAAC GATATTATTG AGGCTCACAG AGAACAGATT GGTGGTACCC TGGAAGCTCA GACCCAGGGT CCTGGTTCGA TGGCCACCGT AGACACCGTC AGGGGGCGGT ACTCGTCCTA CAAGCTGCTC AACGAAGTTG GCCGCGGTGG CAGCGCGCTC GTGTACCGCG CGCAGGACGT GGCCACACGC AAAGATGTTG CCGTGAAGCA GCTCTTTGGC AACCGGCCGC AGGACATGGA AGACTGGCTG CGCGAGGTGG ACGCCCTACA CACGCTCAAC AACCATCATT GCCCGCATGT CGTGAAGTAC CTGGACCACA TGCAGCAGCA CGATCGACTC TTCCTCGTGG AGGAGTTCAC CGAGCACGGA TCGCTGCTGC GCCGTCTGAA GGAAAACAGC AAGCTTCCGG AGGACATCGC TTGCCGGTAT ATCTACCAGG TGCTGACGGC CCTGTATCAT ATGGCCCCGT GGGGCGTCGT GCACGGCGAC TTGAAGGCGT CAAACATTCT TCTCTTCGAC GGCGATGTCG TGAAACTGAC GGACTTTGCA CTGCGCTCGC ATGGCGAGGA CGATCACGAG GATGGCGAGC GGCGCACCAC CGCGGCCTCC ACGCCTTCTG CCGAAGCACT GCCCCTCATG AGGGGCAGCC GCGCCGGGAG GGGCGCTGGT GAAGAGTGCT CTGGCTCTGC CCTCTCGGTG TTCCGCGGCT CCGCTTACTG GGCAGCGCCG GAAGTCCTCG CCGGTGCATC GAAGGCAACG GCGGCGAGCG ACATCTGGTC GGTGGGCTGC CTCGCGGTGG AGTTGCTCAC GGGTGCCCCG CCGTACTTCG AGCGCCCCAT CCACAACGCC ATTCATCACA TCCTCAAGAG CTACTACGAG GTGCTCTGTG AAAGCGAGGC CAGCCGCGTC GGTGCTGCCG GCACCTCGCG CGCGTCCACC AACCCCGCAA CCCCAAGCGC GGCAATGGGC TCTGGCGACG ATGACGGGCC GCGTGGGCCC CGGAAGAAGA AAAACGGAGC GAAAGACGGG TGTGGTGCAG CGCCGCAAAA AACAGACGTG GCGTCCGCGC GGAGGGCGAC GGCAACGACG AGCACCCACA ATCCGCCGAA GGAGCCGGTC GATGCGGTCA AGACAGCCGC ATCTAGCGGC GCTGTGTGTG CATCAGACCG GAATGAAGTG GCCACGCCTC TGTGCGAGGC GGCGCTGCTC CCGCCGCTTC CCGAGGACGT GCACTTGAGC GATGAATGTC TGTCGTTTCT ACGGATGTGC TTCCGCCCCC GCGCGGTAGA CCGGCCGTCG GCCGGAGAGC TGCTCCACGA TCCGTGGTTT CTGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA