LedoB.20202.a

hypothetical protein, conserved

CENTER ID: LedoB.20202.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.20202.a.B1.GE42685 full length 1 491
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LdBPK_150720.1
UniProt: E9BCB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAVFMRVGE LACQKDSFLR ELTATVVSCE PAKTAVAAAK KAKGASSDTV TYDVILSDSV LFPEGGGQPC DHGTLRVVSG DNDKADPSCG ATAAPPLSIT NVRRVGDTCV LTSPAALPVG ATVHQMVDWP RRMDHMQHHT GQHLLTAVVE REDTLRLPTV SWSLTHPYCF IQVDVGGYAS DPASPYHAYI TKEKKISDEM VAKIEVLCND AIAQSTAVQC DVFENREVYM REQDRRHQTA AASADGGTEA FRSRGIPEDV TGPIRIISLD AIDSCTCCGT HLRTLAELQA MYLLHQEVKG TLVKLYFITG KRAARQFHAM YHRERQLMPE LGGSRPNDFV SVVQRRSKDA TDLEKLMKRW TLELASVEAQ RIAAAVPASH AATVTVPLRR DDVDLEFFNE VRARLDAAGL RRVVLVSAWA AERCTAGTLA SAKDVAGQVF VTGGETPADM EKAVQLVKET LEGMKGGTSK LGFRGKGSLK EWDTLVARLK E
NT Sequence
atggcggccg tattcatgcg cgtcggtgag ctggcgtgtc agaaagactc gttcctgcga gagttgacgg cgactgttgt ctcgtgtgag ccagccaaga cggcagtggc agcggcgaag aaggcgaagg gcgccagcag cgacaccgtg acgtacgatg ttattctgag cgactccgtt ctttttccag aaggtggtgg ccagccctgc gaccacggca cactgcgagt ggtgtcaggc gataatgaca aagccgatcc ctcgtgtggc gccaccgccg cgccgccgct ctccattacg aacgtgcgcc gggtcgggga cacatgcgtg ctcaccagtc ccgccgccct ccccgtgggg gctacggtgc accagatggt ggactggccg cgtcggatgg accacatgca gcaccacacc ggccagcacc tgctcactgc cgtggtagag cgcgaggaca cgctgcgctt gcccactgtg agctggtccc tgacgcaccc ttactgtttc atccaggtgg acgttggcgg ctacgcaagc gacccagctt cgccgtacca cgcttacatc acaaaggaga agaaaatatc ggacgaaatg gtggcgaaga tcgaggtgct ctgcaacgac gcgatcgccc agagcaccgc cgtgcagtgc gacgtgttcg aaaatcgaga ggtgtatatg cgggagcagg atcgccggca tcagacggct gcggcctcgg cagacggcgg cacggaagcc tttcgaagcc gcggcatccc agaggacgtc acagggccga tccgcatcat ctcgctggac gccatcgaca gctgcacctg ctgcggcacc cacctgcgca cactagcaga gctgcaggct atgtacctcc tccaccaaga ggttaaagga acgctagtga agctgtactt catcactggc aagcgggcag cgcgacagtt ccacgccatg tatcaccggg agcggcagtt gatgccggag ttgggtggca gcagacccaa cgactttgtc tccgtggtgc agcgccgttc caaggatgcg acggaccttg agaagctgat gaagcggtgg acactggagc tagcgagtgt agaagcacag cgcatcgccg ccgccgtgcc ggcgtcgcac gccgctaccg tcacagtacc cctgcgtcgc gacgacgtag atctcgagtt cttcaacgag gtccgcgcac ggctcgatgc agcagggctg cgccgcgtcg ttctcgtttc cgcgtgggcg gcagagcggt gcacggcagg cacgctcgcc agcgcgaagg atgtggcggg tcaggtcttc gtcactgggg gagagacgcc tgcggacatg gagaaggccg tccagctggt gaaggaaacg ctagagggga tgaagggggg cacctccaag ctgggtttcc gtgggaaggg cagtctgaag gagtgggaca ccctcgtggc gcgtctgaag gagtag
Details for LedoB.20202.a.B1.GE42685
HARVESTED ON: 9/28/2018
SEQUENCED ON: 10/17/2018
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA AVFMRVGELA CQKDSFLREL TATVVSCEPA KTAVAAAKKA KGASSDTVTY DVILSDSVLF PEGGGQPCDH GTLRVVSGDN DKADPSCGAT AAPPLSITNV RRVGDTCVLT SPAALPVGAT VHQMVDWPRR MDHMQHHTGQ HLLTAVVERE DTLRLPTVSW SLTHPYCFIQ VDVGGYASDP ASPYHAYITK EKKISDEMVA KIEVLCNDAI AQSTAVQCDV FENREVYMRE QDRRHQTAAA SADGGTEAFR SRGIPEDVTG PIRIISLDAI DSCTCCGTHL RTLAELQAMY LLHQEVKGTL VKLYFITGKR AARQFHAMYH RERQLMPELG GSRPNDFVSV VQRRSKDATD LEKLMKRWTL ELASVEAQRI AAAVPASHAA TVTVPLRRDD VDLEFFNEVR ARLDAAGLRR VVLVSAWAAE RCTAGTLASA KDVAGQVFVT GGETPADMEK AVQLVKETLE GMKGGTSKLG FRGKGSLKEW DTLVARLKE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg ccgtattcat gcgcgtcggt gagctggcgt gtcagaaaga ctcgttcctg cgagagttga cggcgactgt tgtctcgtgt gagccagcca agacggcagt ggcagcggcg aagaaggcga agggcgccag cagcgacacc gtgacgtacg atgttattct gagcgactcc gttctttttc cagaaggtgg tggccagccc tgcgaccacg gcacactgcg agtggtgtca ggcgataatg acaaagccga tccctcgtgt ggcgccaccg ccgcgccgcc gctctccatt acgaacgtgc gccgggtcgg ggacacatgc gtgctcacca gtcccgccgc cctccccgtg ggggctacgg tgcaccagat ggtggactgg ccgcgtcgga tggaccacat gcagcaccac accggccagc acctgctcac tgccgtggta gagcgcgagg acacgctgcg cttgcccact gtgagctggt ccctgacgca cccttactgt ttcatccagg tggacgttgg cggctacgca agcgacccag cttcgccgta ccacgcttac atcacaaagg agaagaaaat atcggacgaa atggtggcga agatcgaggt gctctgcaac gacgcgatcg cccagagcac cgccgtgcag tgcgacgtgt tcgaaaatcg agaggtgtat atgcgggagc aggatcgccg gcatcagacg gctgcggcct cggcagacgg cggcacggaa gcctttcgaa gccgcggcat cccagaggac gtcacagggc cgatccgcat catctcgctg gacgccatcg acagctgcac ctgctgcggc acccacctgc gcacactagc agagctgcag gctatgtacc tcctccacca agaggttaaa ggaacgctag tgaagctgta cttcatcact ggcaagcggg cagcgcgaca gttccacgcc atgtatcacc gggagcggca gttgatgccg gagttgggtg gcagcagacc caacgacttt gtctccgtgg tgcagcgccg ttccaaggat gcgacggacc ttgagaagct gatgaagcgg tggacactgg agctagcgag tgtagaagca cagcgcatcg ccgccgccgt gccggcgtcg cacgccgcta ccgtcacagt acccctgcgt cgcgacgacg tagatctcga gttcttcaac gaggtccgcg cacggctcga tgcagcaggg ctgcgccgcg tcgttctcgt ttccgcgtgg gcggcagagc ggtgcacggc aggcacgctc gccagcgcga aggatgtggc gggtcaggtc ttcgtcactg ggggagagac gcctgcggac atggagaagg ccgtccagct ggtgaaggaa acgctagagg ggatgaaggg gggcacctcc aagctgggtt tccgtgggaa gggcagtctg aaggagtggg acaccctcgt ggcgcgtctg aaggagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t