LedoB.19509.a

KREPA2

CENTER ID: LedoB.19509.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.19509.a.B1.GE41123 full length 1 626
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: LdBPK_367260.1
RefSeq: XP_003865836.1
UniProt: E9BV82

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTMKRRIGVL AAAAASRCTL RRLPQVSSWP LHASGAVASA KGASNAALLG AVRNQSAVPD RRFHCSVCKK SFRLEMAAKL HLQQVHSGEG TVEAGAGPGQ GEDQALQTPV GVFRNAPPQA PTVVTAIVPD THERAARREK PAPKPLHQAE REVPSLVMEK MLSVWDDTGV KRMGDQFVHS SMVMRVFAAR PSDGAEPLYG VMNPEGENPF EGGPYPPDAG GPLTKNEVNF YSIGIGDAFA LACGESFGPL RPARCPNPFL RKLAKDAAKT APRVGFAEQQ TAPVTPFGQL PMFGQTPSPA TTPAPSAAEE PSTTISVVSS PFAAGANDSP FAGTTDFPFA GQGLSPFGSL KKPADQAAAE PGIAARSAAS FEAPASPFVD AASALTPSPF AAAPSPFTES SSAAAPYAAR DAAAVAGGAE AHDAVFFSSF SASSASRPAE VGPTATHVCT VCKKSFSTYD GLRMHSKAKH GEELPKELRN GRRNEKREVP DLPAFIPSPV DLTVTSPFGS SAQRSAWTEV ELKPYAQSVS NITVAGRVLD SEVSSDGALL LSLFVPDSSE SESEVIPVRC SSAAFRTLGR TLVYGDLVFA CGSLRVLPFT DKKTQKTYSS AVVHVGLPTG MIAKLN
NT Sequence
atgacaatga agaggcggat cggtgtcttg gccgctgctg cagcgagccg ctgcacgctg cgccggcttc cccaagtatc gtcttggccc ttacacgcgt cgggcgctgt cgcctccgcg aaaggtgcct caaacgccgc tctgttgggg gctgtgcgga atcaaagtgc cgtcccggac cggcgcttcc actgtagcgt ttgcaaaaag tcgtttcgct tggagatggc ggcaaagctg caccttcagc aggtgcacag tggtgagggc acggtggagg caggcgctgg accgggacag ggagaggatc aggcgttgca gacaccggtg ggtgtatttc gtaatgcacc tccgcaggca ccaaccgtcg ttacagcgat cgtgccagac actcacgagc gtgccgcgcg ccgtgagaag ccggcaccaa agccacttca ccaagccgaa cgcgaggtgc caagtcttgt gatggaaaag atgctctctg tatgggatga cacgggggtg aagcgtatgg gggatcagtt tgttcacagt agcatggtaa tgcgcgtgtt tgccgctcgc ccgtctgacg gcgccgagcc gttgtacggc gtcatgaatc cggagggtga gaacccgttc gagggtggcc cgtatccccc cgacgcgggt ggtccgctca cgaagaacga ggtcaacttt tacagcattg gtataggtga cgcgtttgcg ctggcgtgtg gtgagtcttt cggtccattg cgtcctgctc ggtgcccgaa cccgttcctc cgaaaactag caaaggacgc tgcaaaaacc gccccgaggg tggggttcgc agagcagcaa acggcgcctg taacgccgtt tggacagctc cccatgtttg ggcagactcc gtcacctgct acgaccccag ctccgagcgc cgccgaggaa ccgtcgacca cgatcagcgt cgtctcttct cccttcgccg ccggcgccaa cgactcgcct tttgctggga cgacggattt tccgtttgct gggcaagggc tctctccgtt cggctcgctg aagaaaccag cagatcaggc cgctgccgag cccgggattg ccgcgcgatc ggcggcctcg tttgaggctc cggcctcacc gtttgtggat gctgctagtg ctctcactcc gtccccgttc gccgcagctc cgagcccttt caccgaatct tcgtcggcag cagccccgta tgctgcgagg gacgcagctg cggtggcagg cggcgctgag gctcatgacg ccgtcttctt ttcctctttc tcagcgtctt ccgcgagccg gccggctgag gtggggccca ccgcgacgca cgtgtgcacc gtttgcaaga agagcttttc gacttatgac ggccttcgaa tgcatagcaa ggccaagcat ggggaggagc tgccgaagga gctgcgaaat gggcggcgaa acgagaagcg cgaggttccg gacttaccgg ccttcattcc cagcccggtg gacctcacgg tgacatcgcc cttcggctcg tcggcgcaac ggtcggcgtg gacggaagtg gagctgaaac cgtacgcgca gtctgtgagt aacattactg tggcgggccg cgtgctggat agcgaagttt cgagtgacgg ggcgttgctt ttgtcgcttt ttgtccccga ttcgtccgag agcgagtcgg aggtgattcc tgttcgctgc agctcagctg cctttagaac gcttggcaga acgctcgtgt atggcgatct cgtgttcgcg tgtggttcgc tgcgcgtttt gccattcacg gataagaaaa cacaaaagac atactcctcg gcggtcgtgc acgtgggtct tccaactggc atgattgcga aactgaactg a
Details for LedoB.19509.a.B1.GE41123
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 66kDa
OBSERVED MW: 66kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMT MKRRIGVLAA AAASRCTLRR LPQVSSWPLH ASGAVASAKG ASNAALLGAV RNQSAVPDRR FHCSVCKKSF RLEMAAKLHL QQVHSGEGTV EAGAGPGQGE DQALQTPVGV FRNAPPQAPT VVTAIVPDTH ERAARREKPA PKPLHQAERE VPSLVMEKML SVWDDTGVKR MGDQFVHSSM VMRVFAARPS DGAEPLYGVM NPEGENPFEG GPYPPDAGGP LTKNEVNFYS IGIGDAFALA CGESFGPLRP ARCPNPFLRK LAKDAAKTAP RVGFAEQQTA PVTPFGQLPM FGQTPSPATT PAPSAAEEPS TTISVVSSPF AAGANDSPFA GTTDFPFAGQ GLSPFGSLKK PADQAAAEPG IAARSAASFE APASPFVDAA SALTPSPFAA APSPFTESSS AAAPYAARDA AAVAGGAEAH DAVFFSSFSA SSASRPAEVG PTATHVCTVC KKSFSTYDGL RMHSKAKHGE ELPKELRNGR RNEKREVPDL PAFIPSPVDL TVTSPFGSSA QRSAWTEVEL KPYAQSVSNI TVAGRVLDSE VSSDGALLLS LFVPDSSESE SEVIPVRCSS AAFRTLGRTL VYGDLVFACG SLRVLPFTDK KTQKTYSSAV VHVGLPTGMI AKLN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacaa tgaagaggcg gatcggtgtc ttggccgctg ctgcagcgag ccgctgcacg ctgcgccggc ttccccaagt atcgtcttgg cccttacacg cgtcgggcgc tgtcgcctcc gcgaaaggtg cctcaaacgc cgctctgttg ggggctgtgc ggaatcaaag tgccgtcccg gaccggcgct tccactgtag cgtttgcaaa aagtcgtttc gcttggagat ggcggcaaag ctgcaccttc agcaggtgca cagtggtgag ggcacggtgg aggcaggcgc tggaccggga cagggagagg atcaggcgtt gcagacaccg gtgggtgtat ttcgtaatgc acctccgcag gcaccaaccg tcgttacagc gatcgtgcca gacactcacg agcgtgccgc gcgccgtgag aagccggcac caaagccact tcaccaagcc gaacgcgagg tgccaagtct tgtgatggaa aagatgctct ctgtatggga tgacacgggg gtgaagcgta tgggggatca gtttgttcac agtagcatgg taatgcgcgt gtttgccgct cgcccgtctg acggcgccga gccgttgtac ggcgtcatga atccggaggg tgagaacccg ttcgagggtg gcccgtatcc ccccgacgcg ggtggtccgc tcacgaagaa cgaggtcaac ttttacagca ttggtatagg tgacgcgttt gcgctggcgt gtggtgagtc tttcggtcca ttgcgtcctg ctcggtgccc gaacccgttc ctccgaaaac tagcaaagga cgctgcaaaa accgccccga gggtggggtt cgcagagcag caaacggcgc ctgtaacgcc gtttggacag ctccccatgt ttgggcagac tccgtcacct gctacgaccc cagctccgag cgccgccgag gaaccgtcga ccacgatcag cgtcgtctct tctcccttcg ccgccggcgc caacgactcg ccttttgctg ggacgacgga ttttccgttt gctgggcaag ggctctctcc gttcggctcg ctgaagaaac cagcagatca ggccgctgcc gagcccggga ttgccgcgcg atcggcggcc tcgtttgagg ctccggcctc accgtttgtg gatgctgcta gtgctctcac tccgtccccg ttcgccgcag ctccgagccc tttcaccgaa tcttcgtcgg cagcagcccc gtatgctgcg agggacgcag ctgcggtggc aggcggcgct gaggctcatg acgccgtctt cttttcctct ttctcagcgt cttccgcgag ccggccggct gaggtggggc ccaccgcgac gcacgtgtgc accgtttgca agaagagctt ttcgacttat gacggccttc gaatgcatag caaggccaag catggggagg agctgccgaa ggagctgcga aatgggcggc gaaacgagaa gcgcgaggtt ccggacttac cggccttcat tcccagcccg gtggacctca cggtgacatc gcccttcggc tcgtcggcgc aacggtcggc gtggacggaa gtggagctga aaccgtacgc gcagtctgtg agtaacatta ctgtggcggg ccgcgtgctg gatagcgaag tttcgagtga cggggcgttg cttttgtcgc tttttgtccc cgattcgtcc gagagcgagt cggaggtgat tcctgttcgc tgcagctcag ctgcctttag aacgcttggc agaacgctcg tgtatggcga tctcgtgttc gcgtgtggtt cgctgcgcgt tttgccattc acggataaga aaacacaaaa gacatactcc tcggcggtcg tgcacgtggg tcttccaact ggcatgattg cgaaactgaa ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat