LedoB.19507.a

KREPB5

CENTER ID: LedoB.19507.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.19507.a.B1.GE41122 full length 1 429
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: LdBPK_270350.1
RefSeq: XP_003861892.1
UniProt: E9BIW9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRTCRSLRR FSAAPLGLAH SICRSAVISS SACAASSLNS VAPRTGSSSF ARQAARMAPP QTTLSLDANL RNTLRSLLIE RLCSPTQPLP AKQLYDELTN RSRLVEEFHF SKTSRRITRQ RLALQFLRRY NVLLHGLLFY SEGTRTWNTT TEFHRLAILG ESVLLTEVRT RLLKLFPAMP YAAYVQSLPH MVGEEALAAA FDRYEMQNIV GAKPSNRRSG TPLTRMQKSH MLCAIVAEMY WFTARTKPTD LTHNNALFPP SDVLILHVLS THLLENLPAE LIYNVVEPIV ADIKRVWVNE PMSLPSQLRL TPRTVCALSL NAVPVEPQYT AKEDAVMSLQ KKHAEAHRRQ HALTCEPDHS CVKSFMRPLC NYRIFEGPRY QILSSDNRVA PLPLAQERSS LPASAVCTVV GDAESAARAM ELAKMAEQW
NT Sequence
atgaagcgga catgtcgctc tctgcgacgg ttttccgcag cgccgcttgg cttagcgcat tcgatctgta gaagcgcggt gatttcttcc tctgcttgcg ctgcctcatc gctaaattcg gtcgcgccgc gcacaggaag ctcgtccttc gcgcgtcagg ccgcgagaat ggcaccgccg cagacaactc tttcactgga cgccaacctg cgcaacaccc tcagaagttt gctcattgag cgtctgtgca gtccgacaca gcccctaccg gcaaagcagc tgtatgatga gctgacgaac cgctcgcgtc tcgtggagga gtttcacttc tcgaagacgt cacgccgcat cacccgccaa cggctcgcac tgcagttctt gcgtcgatac aacgtgctgt tgcacggcct cctcttttat tcggagggca cgcgcacgtg gaatacgacg acggagtttc atcgtctcgc catccttgga gagtctgtcc tactgaccga ggtccgcact cggctgctga agctcttccc tgccatgccg tatgcggcgt acgtgcagag tttgcctcac atggtcggcg aggaagcgct ggcggcggct tttgaccggt atgagatgca gaacattgtc ggtgccaaac catctaaccg acgcagtggt acgccgctca ctcgaatgca gaaaagccat atgctgtgcg ctattgtagc ggagatgtac tggtttacag ctcggacgaa gcccaccgac ttgacgcaca acaacgcgct cttccctcca tccgacgtgc taatcttgca cgtgctgtcc acccacctcc tcgaaaactt gcctgcagag ctcatttaca acgtcgtaga gccaatcgtg gcggacatca agcgcgtgtg ggtgaacgag ccgatgtcgt tgccgtcaca actgcgcctt accccgcgca ctgtatgtgc cctctctctc aatgctgtcc ccgtcgagcc ccagtacacc gccaaggaag acgctgtcat gtcgctccag aaaaagcacg cggaggctca tcgccgacaa cacgcgctta cctgcgagcc ggatcacagc tgcgttaagt ctttcatgcg accgttgtgc aactaccgca tatttgaagg tccacggtat cagatactga gctccgataa tcgcgtcgcc ccactgccgc ttgcacaaga gcggagcagt cttccagctt cggccgtgtg caccgtcgtt ggcgatgcgg aaagcgccgc acgtgcgatg gagcttgcca agatggcgga gcagtggtga
Details for LedoB.19507.a.B1.GE41122
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMK RTCRSLRRIS AAPLGLVHSI CRSAVISSSA CAASSLNSVA PRTGSSSFAR QAARMAPPQT TLSLDANLRN TLRSLLIERL CSPTQPLPAK QLYDELTNRS RLVEEFHFSK TSRRITRQRL ALQFLRRYNV LLHGLLFYSE GTRTWNTTTE FHRLAILGES VLLTEVRTRL LKLFPAMPYA AYVQSLPHMV GEEALAAAFD RYEMQNIVGA KPSNRRSGTP LTRMQKSHML CAIVAEMYWF TARTKPTDLT HNNALFPPSD VLILHVLSTH LLENLPAELI YNVVEPIVAD IKRVWVNEPM SLPSQLRLTP RTVGALSLNA VPVEPRYTAK EDAVMSLQKK HAEAHRRQNA LTCEPDHSCV KSFMRPLCNY RIFEGPRYQI LSSDNRVAPL PLAQERSSLP ASAVCTVVGD AESAARAME
Validated NT Sequence
agctccatcg cacgtgcggc gctttccgca tcgccaacga cggtgcacac ggccgaagct ggaagactgc tccgctcttg tgcaagcggc agtggggcga cgcgattatc ggagctcagt atctgatacc gtggaccttc aaatatgcgg tagttgcaca acggtcgcat gaaagactta acgcagctgt gatccggctc gcaggtaagc gcgttttgtc ggcgatgagc ctccgcgtgc tttttctgga gcgacatgac agcgtcttcc ttggcggtgt accggggctc gacggggaca gcattgagag agagggcacc tacagtgcgc ggggtaaggc gcagttgtga cggcaacgac atcggctcgt tcacccacac gcgcttgatg tccgccacga ttggctctac gacgttgtaa atgagctctg caggcaagtt ttcgaggagg tgggtggaca gcacgtgcaa gattagcacg tcggatggag ggaagagcgc gttgttgtgc gtcaagtcgg tgggcttcgt ccgagctgta aaccagtaca tctccgctac aatagcgcac agcatatggc ttttctgcat tcgagtgagc ggcgtaccac tgcgtcggtt agatggtttg gcaccgacaa tgttctgcat ctcataccgg tcaaaagccg ccgccagcgc ttcctcgccg accatgtgag gcaaactctg cacgtacgcc gcatacggca tggcagggaa gagcttcagc agccgagtgc ggacctcggt cagtaggaca gactctccaa ggatggcgag acgatgaaac tccgtcgtcg tattccacgt gcgcgtgccc tccgaataaa agaggaggcc gtgcaacagc acgttgtatc gacgcaagaa ctgcagtgcg agccgttggc gggtgatgcg gcgtgacgtc ttcgagaagt gaaactcctc cacgagacgc gagcggttcg tcagctcatc atacagctgc tttgccggta ggggctgtgt cggactgcac agacgctcaa tgagcaaact tctgagggtg ttgcgcaggt tggcgtccag tgaaagagtt gtctgcggcg gtgccattct cgcggcctga cgcgcgaagg acgagcttcc tgtgcgcggc gcgaccgaat ttagcgatga ggcagcgcaa gcagaggaag aaatcaccgc gcttctacag atcgaatgca ctaagccaag cggcgctgcg gaaatccgtc gcagagagcg acatgtccgc ttcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMK RTCRSLRRFS AAPLGLAHSI CRSAVISSSA CAASSLNSVA PRTGSSSFAR QAARMAPPQT TLSLDANLRN TLRSLLIERL CSPTQPLPAK QLYDELTNRS RLVEEFHFSK TSRRITRQRL ALQFLRRYNV LLHGLLFYSE GTRTWNTTTE FHRLAILGES VLLTEVRTRL LKLFPAMPYA AYVQSLPHMV GEEALAAAFD RYEMQNIVGA KPSNRRSGTP LTRMQKSHML CAIVAEMYWF TARTKPTDLT HNNALFPPSD VLILHVLSTH LLENLPAELI YNVVEPIVAD IKRVWVNEPM SLPSQLRLTP RTVCALSLNA VPVEPQYTAK EDAVMSLQKK HAEAHRRQHA LTCEPDHSCV KSFMRPLCNY RIFEGPRYQI LSSDNRVAPL PLAQERSSLP ASAVCTVVGD AESAARAMEL AKMAEQW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc ggacatgtcg ctctctgcga cggttttccg cagcgccgct tggcttagcg cattcgatct gtagaagcgc ggtgatttct tcctctgctt gcgctgcctc atcgctaaat tcggtcgcgc cgcgcacagg aagctcgtcc ttcgcgcgtc aggccgcgag aatggcaccg ccgcagacaa ctctttcact ggacgccaac ctgcgcaaca ccctcagaag tttgctcatt gagcgtctgt gcagtccgac acagccccta ccggcaaagc agctgtatga tgagctgacg aaccgctcgc gtctcgtgga ggagtttcac ttctcgaaga cgtcacgccg catcacccgc caacggctcg cactgcagtt cttgcgtcga tacaacgtgc tgttgcacgg cctcctcttt tattcggagg gcacgcgcac gtggaatacg acgacggagt ttcatcgtct cgccatcctt ggagagtctg tcctactgac cgaggtccgc actcggctgc tgaagctctt ccctgccatg ccgtatgcgg cgtacgtgca gagtttgcct cacatggtcg gcgaggaagc gctggcggcg gcttttgacc ggtatgagat gcagaacatt gtcggtgcca aaccatctaa ccgacgcagt ggtacgccgc tcactcgaat gcagaaaagc catatgctgt gcgctattgt agcggagatg tactggttta cagctcggac gaagcccacc gacttgacgc acaacaacgc gctcttccct ccatccgacg tgctaatctt gcacgtgctg tccacccacc tcctcgaaaa cttgcctgca gagctcattt acaacgtcgt agagccaatc gtggcggaca tcaagcgcgt gtgggtgaac gagccgatgt cgttgccgtc acaactgcgc cttaccccgc gcactgtatg tgccctctct ctcaatgctg tccccgtcga gccccagtac accgccaagg aagacgctgt catgtcgctc cagaaaaagc acgcggaggc tcatcgccga caacacgcgc ttacctgcga gccggatcac agctgcgtta agtctttcat gcgaccgttg tgcaactacc gcatatttga aggtccacgg tatcagatac tgagctccga taatcgcgtc gccccactgc cgcttgcaca agagcggagc agtcttccag cttcggccgt gtgcaccgtc gttggcgatg cggaaagcgc cgcacgtgcg atggagcttg ccaagatggc ggagcagtgg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat