LedoB.19505.a

KREX1

CENTER ID: LedoB.19505.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.19505.a.B2.GE42257 5'-3' exonuclease 1 557
LedoB.19505.a.B3.GE42258 endo-exonuclease-phosphatase 680 993
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: LdBPK_260820.1
RefSeq: XP_003861669.1
UniProt: E9BI94

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRGVLARNAC RLNSLQKGTR LADVYQLLIT KKPVEYDYVA IDVNAFVGGA MRITKNMSPE QRRSKEASRH VLNSIMQMLR RVVCRRSLLL AFDGPDTFAK AYKLRTTPST RRLDIQTQRL PGGVLMRAVE DRIVKTMPLG RGLIPGEVVV SGVNVEGPVE RKVTAWALDL ACRDGAQHTS KSICLIGAGE LWMNVLALTP YSQGTSILHG SSDLRHMTLN DSLTWLQLGD DLLTGDARVI TAARTDALLL YLLCHGCSAT DLSPLSGSNF TVLMEAYHAR RREAAAAAGA AAGAPIFQLI REQPNGALVL DVQAFYLLFV AEKDIAAAIQ SLAALVVPAK GASPHAAPLR TFSMPLSTSG GSASEEQMRD ANTPDMLHGS YLSHLLNSHH LFCHGEMLGR FSVPAYMEAE VKMGSLGRSG AAGVRASVVA VGDWTRFLLR CLQRGGRYAF ASDAGAELSY NTSKWAPPAT TAADVSAASS PTAPCTTAYY PFWSSAQPLT AAEYTILSTP NPGILDPLLV DYVPGVTPAL RSELPRALTS VQANLHELRS SLQHFFAYAT ADRPHPSLCL APSYHWCQNE KSKVWSMRYV DLGVVARQQG VRHARGLLPG MSMEQEAPKE GAVVYDAESQ TWTSQPRSFA VFTSSEQLPG HQEREHADGS SSSSMAATAA ASVDAAEVPT LRVLTWNVMF DRYSNQPTPL GMPGIDWCSP KRYPVLAKLI DAEDADVVGM QEVERPFAEY LAAQPWCRER YIMSCSPQSP ILDPWGVLLL VRRGGRWPLQ QLKHLNVPAW SGHVSLMPVA TLDLSAAAAH SDNSGTGGGS SAGKSGSRKL AASPRVVNVC SMHLLAPFVK VNEVARTGQD QALRHALTRQ LQGDTLVMGD FNDWPSNEFL MPPESRYVEC WPIIHPGDYG KTMDESNTFC KLKIEEIFFG RSDKVFLRTG TATASSPTRG VLKPVEAHLV GTRSVNDENG NQEAPAYLFP SDHYGVSMQF QVL
NT Sequence
atgcggggtg tgctggcgcg taacgcatgt cggctcaact ccctccagaa agggacgcgg ctagctgatg tctaccagct cctcatcacc aagaagccgg tcgagtacga ctatgtcgcg attgacgtga acgcctttgt cggcggtgcc atgcgcataa caaagaacat gtcgccagag cagcgtcgca gcaaggaggc ttcgcggcat gtcctcaact ccatcatgca gatgctgcgc cgcgtggtgt gccgtcgctc tcttctcctc gcctttgatg gtccggacac gtttgcgaag gcgtacaagc ttcgcaccac gccgtcgaca cggcgactcg acattcaaac tcagcgcctc cccggtggtg ttctgatgcg tgcggtggaa gatcgcatag tcaagacgat gccgctgggg cgcgggctga ttcccggcga ggtggtcgtt tccggtgtca acgtggaggg cccggtggag cgaaaggtga cggcatgggc tctcgacttg gcgtgccgcg acggggcgca gcacacctcc aagtctatct gcctcatcgg agcgggggag ctgtggatga acgtgctcgc cctcacgccg tactctcaag gtacgagcat cttgcacgga tcgtccgact tgcgccacat gacgctcaac gactccctga catggctcca gctcggcgac gacctgctga ccggcgatgc acgggtcatc acggcagcgc ggacagatgc gctgttgctt tatctgctgt gccacggctg ctccgcaaca gacctctcgc cgctgtctgg atcgaacttt acggttctca tggaagcata ccacgctcga cgccgagaag ccgcagcagc cgccggtgcg gccgccgggg caccgatctt tcagctcatc cgcgagcagc cgaacggtgc cctggtgctt gacgtgcagg cgttctacct actctttgtg gcggaaaagg acattgcagc agcgatccag tcgctggcgg cgcttgtcgt gccggcgaag ggtgcgagcc cacacgcggc accgctgagg accttctcca tgccgctctc cacttcaggc ggcagcgcat cagaggagca gatgcgcgac gctaacacgc ccgacatgtt gcacggcagc tacctaagcc acctgctcaa ctcccatcac ctcttctgcc atggtgaaat gctcggtcgg ttcagtgtgc cggcctacat ggaagcagag gtgaagatgg ggagccttgg ccgcagcggc gctgcgggtg tccgcgccag cgtggtcgcc gtgggggatt ggacccgctt tctgctccgc tgtctccaac gcggggggcg ctacgctttc gccagtgacg caggcgctga gctaagctac aacacctcga agtgggcacc acccgctacg acggctgccg atgtgagcgc tgcgtcttcg cccaccgctc cctgcacgac cgcgtactat cctttctggt cctccgcgca gccgctgacg gcagccgagt acaccatcct tagcacccca aatccaggca tcctcgatcc gctgctagtg gattacgtgc cgggggtaac gccggcgcta cggtcggagc tgccgcgggc gctcacttct gtgcaggcga acctccatga acttcgcagc tcgctgcagc acttcttcgc gtacgcgacg gctgatcggc cgcacccgtc gctgtgcctg gcgccgtcct accactggtg tcaaaacgag aagagcaagg tgtggtcaat gcggtacgtg gacttgggcg tggtcgcacg gcagcagggt gtccgtcacg cacgcggcct gctgcctggc atgagcatgg agcaggaagc gccgaaggag ggcgccgtgg tatatgacgc cgaatcacaa acgtggacct cgcagccacg cagcttcgct gtattcacat cgtcggagca gctaccggga catcaagagc gtgagcatgc cgacgggtca tcgtcttcat ccatggcggc cacggctgct gcttccgtgg atgcggccga ggtgcccacg ctgagggtgc tcacgtggaa cgtcatgttc gatcggtaca gcaaccaacc taccccactc ggtatgccgg gcatcgactg gtgcagcccg aagcggtacc cggtgctggc gaagctcatc gacgccgagg acgccgatgt ggtggggatg caggaagtgg agcggccgtt tgctgagtac ctcgcggcgc agccgtggtg ccgcgagcgg tacatcatgt cctgcagtcc gcagagcccc atcttggatc cgtggggcgt gctgctactt gtgcgccgcg gcgggcggtg gccgctgcag cagctgaaac acctgaatgt gccggcgtgg tcaggccacg tctccctcat gcctgtggct acgctggatt tgagtgcagc cgccgcccac agcgacaaca gtggcaccgg cggtggcagc tcagccggaa agagcggcag cagaaagctt gcagcgtcgc cgcgggtggt gaacgtctgc tccatgcact tgctggcgcc atttgtgaag gtgaacgagg tggcccgcac gggtcaggat caagctctgc gtcatgcgct gacgcggcag ctgcagggcg acacgctcgt gatgggcgac ttcaacgact ggccgagcaa cgagttcctc atgccgccag agtcgcggta cgtcgagtgc tggccgatca ttcaccccgg tgactatggg aaaacaatgg acgagtcgaa caccttctgc aagctgaaga tcgaagagat cttctttggt cgctctgaca aggtgttcct ccggacaggt accgccacag cctcgagccc cacccgaggc gtgctgaagc cagtggaggc gcatctagta ggaacgcgga gcgtcaatga cgaaaacggc aatcaagaag caccggcgta tctgttcccg tctgaccact acggcgtcag catgcagttc caagtcctgt ga
Details for LedoB.19505.a.B2.GE42257
HARVESTED ON: 2/23/2018
SEQUENCED ON: 3/1/2018
EXPECTED MW: 61kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 84
PERCENT COVERAGE: 56
Validated AA Sequence
MAHHHHHHHH MRGVLARNAC RLNSLQKGTR LADVYQLLIT KKPVEYDYVA IDVNAFVGGA MRITKNMSPE QRRSKEASRH VLNSIMQMLR RVVCRRSLLL AFDGPDTFAK AYKLRTTPST RRLDIQTQRL PGGVLMRAVE DRIVKTMPLG RGLIPGEVVV SGVNVEGPVE RKVTAWALDL ACRDGAQHTS KSICLIGAGE LWMNVLALTP YSQGTSILHG SSDLRHMTLX DSLTWLQLGD XXLTGDARVI TAAXXDALLL YLLCHGCSXX XXXXXXXXXX XXXXXXXXXX XREAAAAXXX XRRGTXLSAH XXAAXXXXXA
Validated NT Sequence
tgcgaagttc atggaggttc gcctgcacag aagtgagcgc ccgcggcagc tccgaccgta gcgccggcgt tacccccggc acgtaatcca ctagcagcgg atcgaggatg cctggatttg gggtgctaag gatggtgtac tcggctgccg tcagcggctg cgcggaggac cagaaaggat agtacgcggt cgtgcaggga gcggtgggcg aagacgcagc gctcacatcg gcagccgtcg tagcgggtgg tgcccacttc gaggtgttgt agcttagctc agcgcctgcg tcactggcga aagcgtagcg ccccccgcgt tggagacagc ggagcagaaa gcgggtccaa tcccccacgg cgaccacgct ggcgcggaca cccgcagcgc cgctgcggcc aaggctcccc atcttcacct ctgcttccat gtaggccggc acactgaacc gaccgagcat ttcaccatgg cagaagaggt gatgggagtt gagcaggtgg cttaggtagc tgccgtgcaa catgtcgggc gtgttagcgt cgcgcatctg ctcctctgat gcgctgccgc ctgaagtgga gagcggcatg gagaaggtcc tcagcggtgc cgcgtgtggg ctcgcaccct tcgccggcac gacaagcgcc gccagcgact ggatcgctgc tgcaatgtcc ttttccgccn nnnngagtnn ntagaacgcc ngnnngtcaa gcacnnnnnn nnnnnnnngc tgctnnnnna tgagctgaaa gnnnngtgcc ccggcggnnn nnnnnnncgg ctgctgcggc ttctcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnannnnn aagtnnnnnn nnnncannnn nnagnnnnnn nttnnngagc agccgtggca cagcagataa agcaacagcg catcnnnnng cgctgccgtg atgacccgtg catcgccggt cagcangncg tcgccgagct ggagccatgt cagggagtcg tngagcgtca tgtggcgcaa gtcggacgat ccgtgcaaga tgctcgtacc ttgagagtac ggcgtgaggg cgagcacgtt catccacagc tcccccgctc cgatgaggca gatagacttg gaggtgtgct gcgccccgtc gcggcacgcc aagtcgagag cccatgccgt cacctttcgc tccaccgggc cctccacgtt gacaccggaa acgaccacct cgccgggaat cagaccgcgc cccagcggca tcgtcttgac tatgcgatct tccaccgcac gcatcagaac accaccgggg aggcgctgag tttgaatgtc gagtcgccgt gtcgacggcg tggtgcgaag cttgtacgcc ttcgcaaacg tgtccggacc atcaaaggcg aggagaagag agcgacggca caccacgcgg cgcagcatct gcatgatgga gttgaggaca tgccgcgaag cctccttgct gcgacgctgc tctggcgaca tgttctttgt tatgcgcatg gcaccgccga caaaggcgtt cacgtcaatc gcgacgtagt cgtactcgac cggcttcttg gtgatgagga gctggtagac atcagctagc cgcgtccctt tctggaggga gttgagccga catgcgttac gcgccagcac accccgcata tggtggtggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMR GVLARNACRL NSLQKGTRLA DVYQLLITKK PVEYDYVAID VNAFVGGAMR ITKNMSPEQR RSKEASRHVL NSIMQMLRRV VCRRSLLLAF DGPDTFAKAY KLRTTPSTRR LDIQTQRLPG GVLMRAVEDR IVKTMPLGRG LIPGEVVVSG VNVEGPVERK VTAWALDLAC RDGAQHTSKS ICLIGAGELW MNVLALTPYS QGTSILHGSS DLRHMTLNDS LTWLQLGDDL LTGDARVITA ARTDALLLYL LCHGCSATDL SPLSGSNFTV LMEAYHARRR EAAAAAGAAA GAPIFQLIRE QPNGALVLDV QAFYLLFVAE KDIAAAIQSL AALVVPAKGA SPHAAPLRTF SMPLSTSGGS ASEEQMRDAN TPDMLHGSYL SHLLNSHHLF CHGEMLGRFS VPAYMEAEVK MGSLGRSGAA GVRASVVAVG DWTRFLLRCL QRGGRYAFAS DAGAELSYNT SKWAPPATTA ADVSAASSPT APCTTAYYPF WSSAQPLTAA EYTILSTPNP GILDPLLVDY VPGVTPALRS ELPRALTSVQ ANLHELRSSL QHFFA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcggg gtgtgctggc gcgtaacgca tgtcggctca actccctcca gaaagggacg cggctagctg atgtctacca gctcctcatc accaagaagc cggtcgagta cgactatgtc gcgattgacg tgaacgcctt tgtcggcggt gccatgcgca taacaaagaa catgtcgcca gagcagcgtc gcagcaagga ggcttcgcgg catgtcctca actccatcat gcagatgctg cgccgcgtgg tgtgccgtcg ctctcttctc ctcgcctttg atggtccgga cacgtttgcg aaggcgtaca agcttcgcac cacgccgtcg acacggcgac tcgacattca aactcagcgc ctccccggtg gtgttctgat gcgtgcggtg gaagatcgca tagtcaagac gatgccgctg gggcgcgggc tgattcccgg cgaggtggtc gtttccggtg tcaacgtgga gggcccggtg gagcgaaagg tgacggcatg ggctctcgac ttggcgtgcc gcgacggggc gcagcacacc tccaagtcta tctgcctcat cggagcgggg gagctgtgga tgaacgtgct cgccctcacg ccgtactctc aaggtacgag catcttgcac ggatcgtccg acttgcgcca catgacgctc aacgactccc tgacatggct ccagctcggc gacgacctgc tgaccggcga tgcacgggtc atcacggcag cgcggacaga tgcgctgttg ctttatctgc tgtgccacgg ctgctccgca acagacctct cgccgctgtc tggatcgaac tttacggttc tcatggaagc ataccacgct cgacgccgag aagccgcagc agccgccggt gcggccgccg gggcaccgat ctttcagctc atccgcgagc agccgaacgg tgccctggtg cttgacgtgc aggcgttcta cctactcttt gtggcggaaa aggacattgc agcagcgatc cagtcgctgg cggcgcttgt cgtgccggcg aagggtgcga gcccacacgc ggcaccgctg aggaccttct ccatgccgct ctccacttca ggcggcagcg catcagagga gcagatgcgc gacgctaaca cgcccgacat gttgcacggc agctacctaa gccacctgct caactcccat cacctcttct gccatggtga aatgctcggt cggttcagtg tgccggccta catggaagca gaggtgaaga tggggagcct tggccgcagc ggcgctgcgg gtgtccgcgc cagcgtggtc gccgtggggg attggacccg ctttctgctc cgctgtctcc aacgcggggg gcgctacgct ttcgccagtg acgcaggcgc tgagctaagc tacaacacct cgaagtgggc accacccgct acgacggctg ccgatgtgag cgctgcgtct tcgcccaccg ctccctgcac gaccgcgtac tatcctttct ggtcctccgc gcagccgctg acggcagccg agtacaccat ccttagcacc ccaaatccag gcatcctcga tccgctgcta gtggattacg tgccgggggt aacgccggcg ctacggtcgg agctgccgcg ggcgctcact tctgtgcagg cgaacctcca tgaacttcgc agctcgctgc agcacttctt cgcgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for LedoB.19505.a.B3.GE42258
HARVESTED ON: 2/23/2018
SEQUENCED ON: 3/1/2018
EXPECTED MW: 36kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT LRVLTWNVMF DRYSNQPTPL GMPGIDWCSP KRYPVLAKLI DAEDADVVGM QEVERPFAEY LAAQPWCRER YIMSCSPQSP ILDPWGVLLL VRRGGRWPLQ QLKHLNVPAW SGHVSLMPVA TLDLSAAAAH SDNSGTGGGS SAGKSGSRKL AASPRVVNVC SMHLLAPFVK VNEVARTGQD QALRHALTRQ LQGDTLVMGD FNDWPSNEFL MPPESRYVEC WPIIHPGDYG KTMDESNTFC KLKIEEIFFG RSDKVFLRTG TATASSPTRG VLKPVEAHLV GTRSVNDENG NQEAPAYLFP SDHYGVXXQF QV
Validated NT Sequence
caggacttgg aactgnnnnn ngacgccgta gtggtcagac gggaacagat acgccggtgc ttcttgattg ccgttttcgt cattgacgct ccgcgttcct actagatgcg cctccactgg cttcagcacg cctcgggtgg ggctcgaggc tgtggcggta cctgtccgga ggaacacctt gtcagagcga ccaaagaaga tctcttcgat cttcagcttg cagaaggtgt tcgactcgtc cattgttttc ccatagtcac cggggtgaat gatcggccag cactcgacgt accgcgactc tggcggcatg aggaactcgt tgctcggcca gtcgttgaag tcgcccatca cgagcgtgtc gccctgcagc tgccgcgtca gcgcatgacg cagagcttga tcctgacccg tgcgggccac ctcgttcacc ttcacaaatg gcgccagcaa gtgcatggag cagacgttca ccacccgcgg cgacgctgca agctttctgc tgccgctctt tccggctgag ctgccaccgc cggtgccact gttgtcgctg tgggcggcgg ctgcactcaa atccagcgta gccacaggca tgagggagac gtggcctgac cacgccggca cattcaggtg tttcagctgc tgcagcggcc accgcccgcc gcggcgcaca agtagcagca cgccccacgg atccaagatg gggctctgcg gactgcagga catgatgtac cgctcgcggc accacggctg cgccgcgagg tactcagcaa acggccgctc cacttcctgc atccccacca catcggcgtc ctcggcgtcg atgagcttcg ccagcaccgg gtaccgcttc gggctgcacc agtcgatgcc cggcataccg agtggggtag gttggttgct gtaccgatcg aacatgacgt tccacgtgag caccctcagc gtcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHTL RVLTWNVMFD RYSNQPTPLG MPGIDWCSPK RYPVLAKLID AEDADVVGMQ EVERPFAEYL AAQPWCRERY IMSCSPQSPI LDPWGVLLLV RRGGRWPLQQ LKHLNVPAWS GHVSLMPVAT LDLSAAAAHS DNSGTGGGSS AGKSGSRKLA ASPRVVNVCS MHLLAPFVKV NEVARTGQDQ ALRHALTRQL QGDTLVMGDF NDWPSNEFLM PPESRYVECW PIIHPGDYGK TMDESNTFCK LKIEEIFFGR SDKVFLRTGT ATASSPTRGV LKPVEAHLVG TRSVNDENGN QEAPAYLFPS DHYGVSMQFQ VL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacgctga gggtgctcac gtggaacgtc atgttcgatc ggtacagcaa ccaacctacc ccactcggta tgccgggcat cgactggtgc agcccgaagc ggtacccggt gctggcgaag ctcatcgacg ccgaggacgc cgatgtggtg gggatgcagg aagtggagcg gccgtttgct gagtacctcg cggcgcagcc gtggtgccgc gagcggtaca tcatgtcctg cagtccgcag agccccatct tggatccgtg gggcgtgctg ctacttgtgc gccgcggcgg gcggtggccg ctgcagcagc tgaaacacct gaatgtgccg gcgtggtcag gccacgtctc cctcatgcct gtggctacgc tggatttgag tgcagccgcc gcccacagcg acaacagtgg caccggcggt ggcagctcag ccggaaagag cggcagcaga aagcttgcag cgtcgccgcg ggtggtgaac gtctgctcca tgcacttgct ggcgccattt gtgaaggtga acgaggtggc ccgcacgggt caggatcaag ctctgcgtca tgcgctgacg cggcagctgc agggcgacac gctcgtgatg ggcgacttca acgactggcc gagcaacgag ttcctcatgc cgccagagtc gcggtacgtc gagtgctggc cgatcattca ccccggtgac tatgggaaaa caatggacga gtcgaacacc ttctgcaagc tgaagatcga agagatcttc tttggtcgct ctgacaaggt gttcctccgg acaggtaccg ccacagcctc gagccccacc cgaggcgtgc tgaagccagt ggaggcgcat ctagtaggaa cgcggagcgt caatgacgaa aacggcaatc aagaagcacc ggcgtatctg ttcccgtctg accactacgg cgtcagcatg cagttccaag tcctgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat