LedoB.19504.a

KREPB8

CENTER ID: LedoB.19504.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.19504.a.B1.GE41120 full length 1 387
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: LdBPK_160340.1
RefSeq: XP_003859722.1
UniProt: E9BCN8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHATLLRSAI RTVVPPPKAS PLSRFLRESA AQSVPDADDS TKMTSEPLTS LLPKGSSTES HTLLSSVPVG VFEGLNRHVW VDSKRSFCHL CQETVGNVTI HSSDRDHTSM LFFLLLYTAY PRHDLLTEAA RAPTAGERAA VVVKDGTCAS QSSPPAEHSR AVGVPRPMSA SRNKVGPLFT PKHVLSGAKR HFPSLFRYVT DCRTTDRLHV VDDAVRRAEL EALLLYLAHP PHQALSHVLQ GQSPNGFWYN GERMWKAHIS RIVAQLYPPM SAGMMTNFTQ KCWGRTNGER LYDALCLHRI KAYHGWGPFE SKERKAFFLR HLIWELLCVE VRDEVSEEAK LLTSLALRRL AFEMVFLQSM DYMDRVQRVH ELLGSPSLEE LKSLNAL
NT Sequence
atgcatgcca cgcttctgcg cagtgcgata cgcacggtgg tgccgccccc aaaggcctcg ccgctgtccc ggtttcttcg cgaaagtgcg gcgcagagcg tacccgatgc ggacgactct acgaagatga cgagtgagcc cctgaccagc ttgctgccga aggggtcctc gacggagagc cacaccttgc tctcatcagt gccggtgggc gtgtttgaag gcctgaatcg acatgtctgg gtggatagta agcgcagctt ctgccacctc tgtcaggaga cagtgggtaa cgtcacaatc cacagcagcg accgcgatca taccagcatg ctgtttttcc tgctgctcta cacggcatac ccgagacacg acttgttgac cgaagcggcg cgagccccga cagcaggaga gagggcagcc gttgtggtga aagatgggac ctgcgcctcg cagtcgtctc cgccggccga gcacagcaga gccgtgggtg tgcctcggcc gatgtccgct tcgagaaaca aggtcggccc gctgttcacc cctaagcatg tcctcagcgg ggcgaaacgg cactttccat ctctcttccg ctacgttacg gactgtcgca ccacggatcg cctacacgtc gtcgacgatg ccgtgcgccg tgccgagcta gaggcgctct tgctgtacct cgcgcaccca ccgcaccagg cgctctcgca cgttctgcaa gggcaaagtc cgaacggctt ctggtacaac ggcgagcgca tgtggaaggc gcacatcagc cgcatcgtcg cacaactgta cccacccatg tcggctggca tgatgaccaa cttcacccag aagtgctggg gccgcactaa cggggaacgg ctgtacgacg cactctgcct gcaccgcatc aaggcctacc acggctgggg tcccttcgag agcaaggagc gcaaggcctt ctttctaagg catctaatct gggagctgct ctgtgtcgag gtccgcgacg aggtgtcgga ggaggcgaaa ctgttgacgt cgctggctct tcggcggctt gcgttcgaga tggtgttctt gcagtcaatg gactacatgg atcgagtgca gcgtgtgcac gagctgctgg gcagcccctc actagaggag ctcaaaagtc ttaacgcgct ctga
Details for LedoB.19504.a.B1.GE41120
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMH ATLLRSAIRT VVPPPKASPL SRFLRESAAQ SVPDADDSTK MTSEPLTSLL PKGSSTESHT LLSSVPVGVF EGLNRHVWVD SKRSFCHLCQ ETVGNVTIHS SDRDHTSMLF FLLLYTAYPR HDLLTEAARA PTAGERAAVV VKDGTCASQS SPPAEHSRAV GVPRPMSASR NKVGPLFTPK HVLSGAKRHF PSLFRYVTDC RTTDRLHVVD DAVRRAELEA LLLYLAHPPH QALSHVLQGQ SPNGFWYNGE RMWKAHISRI VAQLYPPMSA GMMTNFTQKC WGRTNGERLY DALCLHRIKA YHGWGPFESK ERKAFFLRHL IWELLCVEVR DEVSEEAKLL TSLALRRLAF EMVFLQSMDY MDRVQRVHEL LGSPSLEELK SLNAL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatg ccacgcttct gcgcagtgcg atacgcacgg tggtgccgcc cccaaaggcc tcgccgctgt cccggtttct tcgcgaaagt gcggcgcaga gcgtacccga tgcggacgac tctacgaaga tgacgagtga gcccctgacc agcttgctgc cgaaggggtc ctcgacggag agccacacct tgctctcatc agtgccggtg ggcgtgtttg aaggcctgaa tcgacatgtc tgggtggata gtaagcgcag cttctgccac ctctgtcagg agacagtggg taacgtcaca atccacagca gcgaccgcga tcataccagc atgctgtttt tcctgctgct ctacacggca tacccgagac acgacttgtt gaccgaagcg gcgcgagccc cgacagcagg agagagggca gccgttgtgg tgaaagatgg gacctgcgcc tcgcagtcgt ctccgccggc cgagcacagc agagccgtgg gtgtgcctcg gccgatgtcc gcttcgagaa acaaggtcgg cccgctgttc acccctaagc atgtcctcag cggggcgaaa cggcactttc catctctctt ccgctacgtt acggactgtc gcaccacgga tcgcctacac gtcgtcgacg atgccgtgcg ccgtgccgag ctagaggcgc tcttgctgta cctcgcgcac ccaccgcacc aggcgctctc gcacgttctg caagggcaaa gtccgaacgg cttctggtac aacggcgagc gcatgtggaa ggcgcacatc agccgcatcg tcgcacaact gtacccaccc atgtcggctg gcatgatgac caacttcacc cagaagtgct ggggccgcac taacggggaa cggctgtacg acgcactctg cctgcaccgc atcaaggcct accacggctg gggtcccttc gagagcaagg agcgcaaggc cttctttcta aggcatctaa tctgggagct gctctgtgtc gaggtccgcg acgaggtgtc ggaggaggcg aaactgttga cgtcgctggc tcttcggcgg cttgcgttcg agatggtgtt cttgcagtca atggactaca tggatcgagt gcagcgtgtg cacgagctgc tgggcagccc ctcactagag gagctcaaaa gtcttaacgc gctctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat