LedoB.19144.a

Uncharacterized protein

CENTER ID: LedoB.19144.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.19144.a.B2.GE39103 soluble domain 45 304
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:LdBPK_343700.1
RefSeq: XP_003864529.1
UniProt: E9BRG8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGHHADPNKY VAYRDATVLQ RRIATFVLVF SVMIIALVMT FSSVQHREAP CQDRAHEVEF DFMIRPDSTH EAIVTALQTI LEKRRCWLDF APQNDDAPTM VQLNVLDTTT GLIAGRGFRV VRRSDGSNYH YELRAVFDKL CGTYPPISME VMANVDYERV TYNIKAASLM NSTVKYLQHS SLLTKEKNRV TTVTQLQSVF PGFHQLGPST ARLSTIQSAK YTVEGVSQVY FNGSPLDIRV RVQHWLSDKG TPDFWRVVLS TQNIMAERDL VSLQSSIREG LTKLNLLCNA TSSSCANELD LYLR
NT Sequence
atggggcatc atgcagatcc aaacaagtac gtggcatacc gcgacgccac ggtgctgcag cggcgcatcg cgacctttgt actggtgttt tcggtcatga ttatcgccct tgtaatgacc ttctcgtcag ttcagcaccg cgaggccccg tgccaggatc gcgcgcacga ggtcgagttt gacttcatga tccgtccaga ctccacccac gaggccatag tgacagcgct gcagacgatc ctggaaaagc gtcgctgctg gctggacttt gctcctcaga atgacgatgc gccgacaatg gtacagctga acgtactcga caccacgact ggccttatag ccgggcgtgg cttccgtgtg gtgcgtcgca gtgacggctc aaactatcac tacgagcttc gcgccgtctt cgacaaacta tgcggcacct atccacccat ctccatggag gtgatggcca acgtggacta cgaaagggtg acgtacaaca tcaaagccgc ctccctgatg aacagcacag tgaaatactt gcagcacagc tcgttgctca cgaaggagaa gaaccgcgtg acaaccgtga cgcagctgca gtcggtgttt ccaggttttc atcagttggg ccccagcaca gcgcgtttat cgactattca gtccgccaag tacacggtgg agggtgtttc gcaggtatac ttcaacggca gcccactcga catccgcgtc cgtgtgcagc actggctatc tgacaaaggt acgccggact tctggcgcgt cgtgctgagc acgcagaaca tcatggcgga gcgggatctt gtctccctgc agtctagcat tcgagaaggc ttaaccaaac taaacctgct gtgcaacgcg acgtcgtcga gctgcgccaa cgagctcgat ctctacctcc ggtga
Details for LedoB.19144.a.B2.GE39103
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMQ HREAPCQDRA HEVEFDFMIR PDSTHEAIVT ALQTILEKRR CWLDFAPQND DAPTMVQLNV LDTTTGLIAG RGFRVVRRSD GSNYHYELRA VFDKLCGTYP PISMEVMANV DYERVTYNIK AASLMNSTVK YLQHSSLLTK EKNRVTTVTQ LQSVFPGFHQ LGPSTARLST IQSAKYTVEG VSQVYFNGSP LDIRVRVQHW LSDKGTPDFW RVVLSTQNIM AERDLVSLQS SIREGLTKLN LLCNATSSSC ANELDLYLR
Validated NT Sequence
atcttactca cttaccggag gtagagatcg agctcgttgg cgcagctcga cgacgtcgcg ttgcacagca ggtttagttt ggttaagcct tctcgaatgc tagactgcag ggagaccaga tcccgctccg ccatgatgtt ctgcgtgctc agcacgacgc gccagaagtc cggcgtacct ttgtcagata gccagtgctg cacacggacg cggatgtcga gtgggctgcc gttgaagtat acctgcgaaa caccctccac cgtgtacttg gcggactgaa tagtcgataa acgcgctgtg ctggggccca actgatgaaa acctggaaac accgactgca gctgcgtcac ggttgtcacg cggttcttct ccttcgtgag caacgagctg tgctgcaagt atttcactgt gctgttcatc agggaggcgg ctttgatgtt gtacgtcacc ctttcgtagt ccacgttggc catcacctcc atggagatgg gcggataggt gccgcatagt ttgtcgaaga cggcgcgaag ctcgtagtga tagtttgagc cgtcactgcg acgcaccaca cggaagccac gcccggctat aaggccagtc gtggtgtcga gtacgttcag ctgtaccatt gtcggcgcat cgtcattctg aggagcaaag tccagccagc agcgacgctt ttccaggatc gtctgcagcg ctgtcactat ggcctcgtgg gtggagtctg gtcggatcat gaagtcaaac tcgacctcgt gcgcgcgatc ctggcacggg gcctcgcggt gctgcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHQH REAPCQDRAH EVEFDFMIRP DSTHEAIVTA LQTILEKRRC WLDFAPQNDD APTMVQLNVL DTTTGLIAGR GFRVVRRSDG SNYHYELRAV FDKLCGTYPP ISMEVMANVD YERVTYNIKA ASLMNSTVKY LQHSSLLTKE KNRVTTVTQL QSVFPGFHQL GPSTARLSTI QSAKYTVEGV SQVYFNGSPL DIRVRVQHWL SDKGTPDFWR VVLSTQNIMA ERDLVSLQSS IREGLTKLNL LCNATSSSCA NELDLYLR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcagcacc gcgaggcccc gtgccaggat cgcgcgcacg aggtcgagtt tgacttcatg atccgtccag actccaccca cgaggccata gtgacagcgc tgcagacgat cctggaaaag cgtcgctgct ggctggactt tgctcctcag aatgacgatg cgccgacaat ggtacagctg aacgtactcg acaccacgac tggccttata gccgggcgtg gcttccgtgt ggtgcgtcgc agtgacggct caaactatca ctacgagctt cgcgccgtct tcgacaaact atgcggcacc tatccaccca tctccatgga ggtgatggcc aacgtggact acgaaagggt gacgtacaac atcaaagccg cctccctgat gaacagcaca gtgaaatact tgcagcacag ctcgttgctc acgaaggaga agaaccgcgt gacaaccgtg acgcagctgc agtcggtgtt tccaggtttt catcagttgg gccccagcac agcgcgttta tcgactattc agtccgccaa gtacacggtg gagggtgttt cgcaggtata cttcaacggc agcccactcg acatccgcgt ccgtgtgcag cactggctat ctgacaaagg tacgccggac ttctggcgcg tcgtgctgag cacgcagaac atcatggcgg agcgggatct tgtctccctg cagtctagca ttcgagaagg cttaaccaaa ctaaacctgc tgtgcaacgc gacgtcgtcg agctgcgcca acgagctcga tctctacctc cggtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat