SSGCID: Target Page

LedoB.19141.a

Uncharacterized protein

CENTER ID:	LedoB.19141.a
ORGANISM:	Leishmania donovani BPK282A1
ASSOCIATED DISEASE:	Leishmaniasis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LedoB.19141.a.B1.GE39101	full length		1	437

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LdBPK_211850.1
RefSeq:	XP_003860702.1
UniProt:	E9BFH1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKSVKKTPKG EHHSLVPTTT TAAANSTSNG TKDHIHNGNN AAASQRTSVP LLDEESLKAN TASASSGAAG GFTLVPHSDD RLKMGGATIS GSPPSQPHSV DSSNIYTMLQ QQHAVAMAAA QLEHAYNQTC YLLQQCSHNA LLTVLMSLLN DYPTTLVQPI RMRVEQVLAH QMSPSASGRV AVPGRRGSEF VPNSSAAPWT PSNMRGSAAP TPTANSTTPS HRRGRGHKGK EEEQEMCSIH NNLRAMKHLQ LNVSTGLYEC IHGFHCLVDG PTDASPKRLS TASAVSPSTS ASRANGPAPS ADSQPFAPPP TIVSPAAAES RFSVTEVEAL RMLGYPLPNT NSDQPQPHLA HESPHPQPTL QQPHQQFSHL SLRSSGLGLS GPSHHDPESS QLANGGGFTV ISGERSSAGN SPLDARLSMD IPSLEYLLQS VRDIDDD

NT Sequence

atgaagtctg tcaagaagac cccaaagggg gaacaccaca gcctcgtgcc gacgacgacc accgccgcgg ctaatagcac cagcaacggc actaaagacc atatccacaa tggaaacaac gccgccgcgt ctcagcgcac atcggtgccg ctgctggacg aggagagtct aaaggcgaac accgctagcg cctctagtgg cgcagcaggt ggcttcactc tagtgccgca cagcgacgat agactaaaaa tgggcggcgc cacaatctct gggtcgccgc ctagccagcc gcacagcgtg gacagtagca acatctacac gatgctgcag cagcagcacg cggtagccat ggccgcggcg cagttggagc acgcgtacaa ccagacatgc tacttgctgc agcagtgctc gcataacgca ctcctcacgg tgctcatgtc gctgctgaac gactacccca ccaccctagt ccagccaatc cgcatgcgag tggaacaagt gttggcacat cagatgtccc cgtccgcctc gggccgcgtg gcggtgccag ggagaagagg cagcgaattt gtgccaaaca gcagcgccgc gccttggacg ccatcaaaca tgcgtggcag cgccgccccg acgccgacag cgaacagcac tacccctagt caccgtcgcg gtcggggaca caagggtaag gaggaggagc aggagatgtg cagcatccac aacaacttgc gggcgatgaa gcacctccaa ctgaacgtct ccactggact gtacgagtgc atacacggct ttcactgcct cgtcgacggt cccacggacg cgtctccgaa gcggctgagc acagcctcgg cggtgtcgcc ttccacctct gcttcccgcg ccaacggccc tgcaccgtct gccgacagcc aaccgtttgc ccctcccccc accatcgtct caccggctgc ggcggagtcg cgcttcagtg taaccgaggt cgaggcgctg cgcatgttgg ggtaccctct gccgaacacc aactctgacc aaccgcagcc gcaccttgcc catgagtcgc ctcacccgca gccgacactg cagcagcctc accagcagtt ctcgcaccta tccctgcgca gctcgggtct cggcctcagt ggccccagcc accacgaccc cgagagctca cagctcgcca acgggggcgg attcaccgtg atcagcggcg aacggtcgag cgccggcaac tcgccccttg atgcccgtct cagcatggat ataccctcgc tggagtacct tctgcagagc gtacgtgata tcgatgacga ctaa

Details for LedoB.19141.a.B1.GE39101

HARVESTED ON:	3/31/2015
SEQUENCED ON:	3/27/2015
EXPECTED MW:	47kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMK SVKKTPKGEH HSLVPTTTTA AANSTSNGTK DHIHNGNNAA ASQRTSVPLL DEESLKANTA SASSGAAGGF TLVPHSDDRL KMGGATISGS PPSQPHSVDS SNIYTMLQQQ HAVAMAAAQL EHAYNQTCYL LQQCSHNALL TVLMSLLNDY PTTLVQPIRM RVEQVLAHQM SPSASGRVAV PGRRGSEFVP NSSAAPWTPS NMRGSAAPTP TANSTTPSHR RGRGHKGKEE EQEMCSIHNN LRAMKHLQLN VSTGLYECIH GFHCLVDGPT DASPKRLSTA SAVSPSTSAS RANGPAPSAD SQPFAPPPTI VSPAAAESRF SVTEVEALRM LGYPLPNTNS DQPQPHLAHE SPHPQPTLQQ PHQQFSHLSL RSSGLGLSGP SHHDPESSQL ANGGGFTVIS GERSSAGNSP LDARLSMDIP SLEYLLQS

Validated NT Sequence

cgctctgcag aaggtactcc agcgagggta tatccatgct gagacgggca tcaaggggcg agttgccggc gctcgaccgt tcgccgctga tcacggtgaa tccgcccccg ttggcgagct gtgagctctc ggggtcgtgg tggctggggc cactgaggcc gagacccgag ctgcgcaggg ataggtgcga gaactgctgg tgaggctgct gcagtgtcgg ctgcgggtga ggcgactcat gggcaaggtg cggctgcggt tggtcagagt tggtgttcgg cagagggtac cccaacatgc gcagcgcctc gacctcggtt acactgaagc gcgactccgc cgcagccggt gagacgatgg tggggggagg ggcaaacggt tggctgtcgg cagacggtgc agggccgttg gcgcgggaag cagaggtgga aggcgacacc gccgaggctg tgctcagccg cttcggagac gcgtccgtcg gaccgtcgac gaggcagtga aagccgtgta tgcactcgta cagtccagtg gagacgttca gttggaggtg cttcatcgcc cgcaagttgt tgtggatgct gcacatctcc tgctcctcct ccttaccctt gtgtccccga ccgcgacggt gactaggggt agtgctgttc gctgtcggcg tcggggcggc gctgccacgc atgtttgatg gcgtccaagg cgcggcgctg ctgtttggca caaattcgct gcctcttctc cctggcaccg ccacgcggcc cgaggcggac ggggacatct gatgtgccaa cacttgttcc actcgcatgc ggattggctg gactagggtg gtggggtagt cgttcagcag cgacatgagc accgtgagga gtgcgttatg cgagcactgc tgcagcaagt agcacgtctg gttgtacgcg tgctccaact gcgccgcggc catggctacc gcgtgctgct gctgcagcat cgtgtagatg ttgctactgt ccacgctgtg cggctggcta ggcggcgacc cagagattgt ggcgccgccc atttttagtc tatcgtcgct gtgcggcact agagtgaagc cacctgctgc gccactagag gcgctagcgg tgttcgcctt tagactctcc tcgtccagca gcggcaccga tgtgcgctga gacgcggcgg cgttgtttcc attgtggata tggtctttag tgccgttgct ggtgctatta gccgcggcgg tggtcgtcgt cggcacgagg ctgtggtgtt ccccctttgg ggtcttcttg acagacttca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagt ctgtcaagaa gaccccaaag ggggaacacc acagcctcgt gccgacgacg accaccgccg cggctaatag caccagcaac ggcactaaag accatatcca caatggaaac aacgccgccg cgtctcagcg cacatcggtg ccgctgctgg acgaggagag tctaaaggcg aacaccgcta gcgcctctag tggcgcagca ggtggcttca ctctagtgcc gcacagcgac gatagactaa aaatgggcgg cgccacaatc tctgggtcgc cgcctagcca gccgcacagc gtggacagta gcaacatcta cacgatgctg cagcagcagc acgcggtagc catggccgcg gcgcagttgg agcacgcgta caaccagaca tgctacttgc tgcagcagtg ctcgcataac gcactcctca cggtgctcat gtcgctgctg aacgactacc ccaccaccct agtccagcca atccgcatgc gagtggaaca agtgttggca catcagatgt ccccgtccgc ctcgggccgc gtggcggtgc cagggagaag aggcagcgaa tttgtgccaa acagcagcgc cgcgccttgg acgccatcaa acatgcgtgg cagcgccgcc ccgacgccga cagcgaacag cactacccct agtcaccgtc gcggtcgggg acacaagggt aaggaggagg agcaggagat gtgcagcatc cacaacaact tgcgggcgat gaagcacctc caactgaacg tctccactgg actgtacgag tgcatacacg gctttcactg cctcgtcgac ggtcccacgg acgcgtctcc gaagcggctg agcacagcct cggcggtgtc gccttccacc tctgcttccc gcgccaacgg ccctgcaccg tctgccgaca gccaaccgtt tgcccctccc cccaccatcg tctcaccggc tgcggcggag tcgcgcttca gtgtaaccga ggtcgaggcg ctgcgcatgt tggggtaccc tctgccgaac accaactctg accaaccgca gccgcacctt gcccatgagt cgcctcaccc gcagccgaca ctgcagcagc ctcaccagca gttctcgcac ctatccctgc gcagctcggg tctcggcctc agtggcccca gccaccacga ccccgagagc tcacagctcg ccaacggggg cggattcacc gtgatcagcg gcgaacggtc gagcgccggc aactcgcccc ttgatgcccg tctcagcatg gatataccct cgctggagta ccttctgcag agcgtacgtg atatcgatga cgactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat