LedoB.19040.a

Uncharacterized protein

CENTER ID: LedoB.19040.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.19040.a.B2.GE39095 potential crystallizable domain #1 36 290
LedoB.19040.a.B3.GE39096 potential crystallizable domain #2 718 874
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:LdBPK_100850.1
RefSeq: XP_003858921.1
UniProt: E9BAD0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRRGLLQAA AAAAATAAVI SVRYSSSSSK NGQLWITVCC GTSTAFLSHP SGGRAMIRVP LELAQGMILP GGTNTRAAST GGCGSYHPSA LHASRVLLAL RSTPPHSSSS AVLSSALASA GATHHIVNSH TAEDERGPFE VTIDEVLLHS HDHHRNGAAA AVDTVADLVR LLVDPDLEVG GGAVEAVSLR DFAAMTRKRK LRAVGGDAEC LEVLLNSERY QSEKDARELR LRGVATLGQW KEQPDRVQLS AYTRGVLETA YSRLARHRHR SQTDEVMAWL TRAIQRAVAD ELMAPKRLTD GAAMADPSAP PYLVDVKASA VRDAEAAMAS TAAEQGNDNE GPAAVAGPSG DSSRNEVGDE SVAARRRGPR QRPVVSGVMP KASKVAALEE DGVAESEEVA DMLDENAEEV LEEGEEMVPI MEEERPARRS AASRSPQPSP AAAATSAGRH GSKRAPAAAP TAPRQQQRQP PPSQDVEAEV EAADVDEDDE ELVEEVEEEV EEAEEEEAAA TTAAPSSTAP AQPSSPLAPA TAPAPPAAAA TAAVGATAAE RRERLVRLAE LMMKQFNTAD GYLHPTNKAE REAQEQQGDI LVLDEDTAQV DPLAMFSTYH AATITDADPV GVRALKTIWT SYNKHQMALE EATDDAAVEF YKRESVNALL YGSVLMRALA REEQVVVLEG TPLPPYGFPL VSSDTRPFAV FAAAGASSSS SATAVTDMTP GKVLTAVQAY KMFFNSKEKR YSPFRPAIDQ SAGCSVVRLS GTTLHLYYTS TKDHIIEEDV RLPFAEVMLG VLHLLRHKVL PRVNVVHYHL IATKMADPSD STDFMLRSTA TIDLTNPFTK EELARLRALA TPLGMADEMD EYRCIDDLVM DIEDSSGASV VHSLLHNRED LEATLTATLA QLLPEDGDRA AGAAEAAEDA EEKDEEDVEV AAPPPPSRKA RGAAAAPASS PSHGHTSRRR AVPEDDEEDT VRVADEQAAE AVPARTSRGK HRQSAGTPLP TAAAAAHASR NREQEEAVLE EAIYNQWQQQ RGTSKTGAAA ATTPKSMAVA QHSDDDRASA EEAEEDFDEE YEEVEELQEL EEAEKELPPA RASARSSLPS ASAAAGAGAR ARGPGAASPA PPASQAQGSS RAVAAVHAAA AYEEEYEEDG VEKEEEEDEE MEVVVAPTIP RAPAGRRAVP VAAAPARPSP PPTPPSPEVR RAPQKLDDDE KALQVRTRRG ARRSTARARA LPPAAHEDVE ETAATAVEDS PEVSAEDADE DQWFKKRPKR RYFQ
NT Sequence
atgctccgaa gaggtcttct tcaagcagcc gcagccgcag cggcgacagc ggcggtcatc tcagtgcgct acagcagcag cagcagcaag aatggtcagc tgtggattac agtctgttgt ggaacgtcga ctgctttttt gtcacacccc tccggtggca gggcaatgat ccgtgttccg ctggagctcg cacaaggaat gattttacca ggcggcacaa acaccagggc ggccagtacc ggcggctgcg gctcatatca cccgagtgcg cttcacgcgt ctcgcgtcct tctcgcgctc cgctccaccc cgccccacag cagcagcagc gccgtcctgt ccagcgcact cgcctccgct ggtgccaccc accacatcgt caactcgcac accgcggagg acgagcgcgg gccgtttgag gtgaccatcg acgaagtcct gctgcattcg catgatcatc acaggaatgg cgctgctgct gcagtcgaca ctgttgccga cctcgtccgg ctccttgtgg accccgactt ggaggtcggc ggtggtgccg tggaggcggt cagtctccgc gactttgctg ccatgacacg gaagcggaag ctcagggccg tcggcggcga cgcggagtgc ctggaggtgc tgctcaacag cgagcgatac caaagcgaaa aagacgcgcg tgagctgcgc ctgcgcggtg tggcgacgct cgggcagtgg aaggagcagc cagaccgagt gcaactcagc gcgtacacac ggggtgtcct cgaaacggcc tacagccgcc tcgctcgcca ccggcaccgg tcgcaaacgg atgaggtgat ggcgtggctg acccgcgcga tccagcgcgc cgtcgccgat gagctgatgg cgccgaagcg gttgacagac ggcgctgcca tggccgaccc gtcagcacca ccctacctgg tggacgtgaa agcctctgcc gtcagggatg ccgaagcggc catggcgagc accgcagcgg agcaaggcaa tgataacgaa ggccctgcgg cggtggcagg cccatctggc gactcttcgc gcaacgaggt tggcgacgaa agcgtcgcag cgcggcgtcg cggtcctcga cagcgccctg tcgtctcggg cgtaatgcca aaggcgtcta aggtcgctgc gttggaagag gacggtgttg ccgaaagcga ggaggtggcg gatatgcttg acgagaacgc cgaggaagtg ctggaagaag gcgaggagat ggtgccgata atggaagagg aaaggccggc aaggcgctcc gcggcatcac gcagcccgca gccgtcccct gccgcagcag ctacgtcagc aggtcggcac ggcagcaaga gagcacctgc ggccgctccc acggcacctc ggcagcagca gcgccagccg ccaccttcac aagacgtcga agcagaggtg gaagctgcgg acgtggacga ggacgatgag gaactcgtcg aggaggtcga ggaagaagtc gaagaggcgg aggaagagga agccgcagcg acgacggcgg cgccctcgtc caccgcaccg gcgcaacctt catcaccact agcccccgct acagcaccag cgccgccggc cgcagcagcg acagcagcag tcggggcgac cgcggcagag aggcgggaaa ggctggtccg acttgcagag ctgatgatga agcagttcaa caccgctgac ggctaccttc acccgaccaa caaggcggag cgggaggcgc aggagcagca gggcgacatt ctggtgctcg acgaggacac cgcgcaggtc gacccactcg ccatgttcag cacctaccac gctgccacga tcacagacgc cgacccggtc ggcgtacggg cactcaagac gatctggacg tcatacaaca agcaccagat ggcactggag gaggcaacag atgacgcagc tgtcgagttc tacaagcgcg agagcgtgaa cgcgctcctg tacggctccg tcctgatgcg ggcactggcg cgcgaggagc aggtcgtcgt gctcgagggc acgccgttgc cgccttacgg gttcccgcta gtatcgtcgg acacacggcc ctttgccgtc tttgccgcgg ccggggcgtc atcgtcgtcc tcggcaacgg ccgtcactga catgacacct ggaaaggtgc tcaccgccgt ccaggcgtac aagatgttct tcaactcgaa ggagaagcgc tactccccgt tccgcccagc gatcgatcag agcgccggct gcagcgtcgt tcgcctttcc ggaaccacgc tgcacctcta ctacacgtct acgaaagacc acatcatcga ggaagacgtg cggctgccct ttgcggaggt gatgctcggt gtcctgcacc tgttacgcca caaggtgctt ccccgcgtga acgtggtgca ctaccacctc atcgcaacca agatggcaga cccgtcagac agcacggact tcatgctgcg ctcgacagcg acgatcgact tgacgaaccc gtttacaaag gaggaactcg ctcgactgcg ggcgctcgcg accccgctcg ggatggcgga tgagatggac gagtaccgct gcatcgatga cctcgtgatg gacatcgagg attcctccgg ggcctcggtg gtgcacagtc tgcttcacaa tcgcgaggac ttggaggcca cactgaccgc gacgctggcg caactgcttc cagaggatgg cgaccgcgcc gcgggtgcgg cagaggcggc cgaagacgcg gaggagaaag acgaagagga cgtggaagta gctgcgccgc caccgccctc acgcaaggcg agaggtgctg cagcggctcc tgcctcatcg ccgagccacg gccacactag tcgccgtcgc gctgtgccag aggacgacga ggaagacacg gtgcgtgtgg cggacgaaca ggccgctgag gcggtgccgg cacgcacgtc gcgagggaag caccgccagt cggcagggac acccttgccc acggcggcgg cagcagcaca cgcttcgcgg aacagagagc aagaggaggc ggtgctcgaa gaggccatct acaaccagtg gcagcaacaa cgcggaacga gcaagaccgg tgcagcggcg gcaacgacgc ccaagtcaat ggccgtcgcg cagcacagtg acgacgaccg cgcttcggca gaggaagcgg aagaggactt cgacgaagaa tacgaggagg tcgaagagct gcaagagctt gaagaggcgg agaaagaact tccacctgca cgtgcctctg cccgttcttc cctcccttcc gcctccgcag cagccggcgc cggtgcgcgt gcacgtggcc ccggcgccgc ctcacccgcg ccgccggctt cacaagcaca aggaagcagc agggccgtag cagcagtaca tgccgctgct gcgtacgagg aggagtacga agaagacggt gtcgagaaag aagaggagga agacgaggag atggaagtgg tagtagcgcc aacgatcccg cgggcccctg ctgggcgtcg cgctgtccct gtcgcagctg ccccggcgcg gccgtcaccg ccgcctacgc caccgtcacc ggaggtccga cgcgcaccgc agaagctgga cgacgacgag aaggctctgc aggtgcgcac gcgtcgtggt gcccgccgat ccaccgcacg cgcacgcgca cttccaccgg cggcccatga ggacgttgag gagaccgcgg caacagcggt tgaagattcg ccggaggtgt cggcagagga cgcggacgag gatcagtggt ttaagaagcg tccgaagcgt cgctacttcc agtaa
Details for LedoB.19040.a.B2.GE39095
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMI TVCCGTSTAF LSHPSGGRAM IRVPLELAQG MILPGGTNTR AASTGGCGSY HPSALHASRV LLALRSTPPH SSSSAVLSSA LASAGATHHI VNSHTAEDER GPFEVTIDEV LLHSHDHHRN GAAAAVDTVA DLVRLLVDPD LEVGGGAVEA VSLRDFAAMT RKRKLRAVGG DAECLEVLLN SERYQSEKDA RELRLRGVAT LGQWKEQPDR VQLSAYTRGV LETAYSRLAR HRHRSQTDEV MAWLTRAIQR AVAD
Validated NT Sequence
atcttactca cttaatcggc gacggcgcgc tggatcgcgc gggtcagcca cgccatcacc tcatccgttt gcgaccggtg ccggtggcga gcgaggcggc tgtaggccgt ttcgaggaca ccccgtgtgt acgcgctgag ttgcactcgg tctggctgct ccttccactg cccgagcgtc gccacaccgc gcaggcgcag ctcacgcgcg tctttttcgc tttggtatcg ctcgctgttg agcagcacct ccaggcactc cgcgtcgccg ccgacggccc tgagcttccg cttccgtgtc atggcagcaa agtcgcggag actgaccgcc tccacggcac caccgccgac ctccaagtcg gggtccacaa ggagccggac gaggtcggca acagtgtcga ctgcagcagc agcgccattc ctgtgatgat catgcgaatg cagcaggact tcgtcgatgg tcacctcaaa cggcccgcgc tcgtcctccg cggtgtgcga gttgacgatg tggtgggtgg caccagcgga ggcgagtgcg ctggacagga cggcgctgct gctgctgtgg ggcggggtgg agcggagcgc gagaaggacg cgagacgcgt gaagcgcact cgggtgatat gagccgcagc cgccggtact ggccgccctg gtgtttgtgc cgcctggtaa aatcattcct tgtgcgagct ccagcggaac acggatcatt gccctgccac cggaggggtg tgacaaaaaa gcagtcgacg ttccacaaca gactgtaatc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHIT VCCGTSTAFL SHPSGGRAMI RVPLELAQGM ILPGGTNTRA ASTGGCGSYH PSALHASRVL LALRSTPPHS SSSAVLSSAL ASAGATHHIV NSHTAEDERG PFEVTIDEVL LHSHDHHRNG AAAAVDTVAD LVRLLVDPDL EVGGGAVEAV SLRDFAAMTR KRKLRAVGGD AECLEVLLNS ERYQSEKDAR ELRLRGVATL GQWKEQPDRV QLSAYTRGVL ETAYSRLARH RHRSQTDEVM AWLTRAIQRA VAD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catattacag tctgttgtgg aacgtcgact gcttttttgt cacacccctc cggtggcagg gcaatgatcc gtgttccgct ggagctcgca caaggaatga ttttaccagg cggcacaaac accagggcgg ccagtaccgg cggctgcggc tcatatcacc cgagtgcgct tcacgcgtct cgcgtccttc tcgcgctccg ctccaccccg ccccacagca gcagcagcgc cgtcctgtcc agcgcactcg cctccgctgg tgccacccac cacatcgtca actcgcacac cgcggaggac gagcgcgggc cgtttgaggt gaccatcgac gaagtcctgc tgcattcgca tgatcatcac aggaatggcg ctgctgctgc agtcgacact gttgccgacc tcgtccggct ccttgtggac cccgacttgg aggtcggcgg tggtgccgtg gaggcggtca gtctccgcga ctttgctgcc atgacacgga agcggaagct cagggccgtc ggcggcgacg cggagtgcct ggaggtgctg ctcaacagcg agcgatacca aagcgaaaaa gacgcgcgtg agctgcgcct gcgcggtgtg gcgacgctcg ggcagtggaa ggagcagcca gaccgagtgc aactcagcgc gtacacacgg ggtgtcctcg aaacggccta cagccgcctc gctcgccacc ggcaccggtc gcaaacggat gaggtgatgg cgtggctgac ccgcgcgatc cagcgcgccg tcgccgattg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for LedoB.19040.a.B3.GE39096
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 19kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT PGKVLTAVQA YKMFFNSKEK RYSPFRPAID QSAGCSVVRL SGTTLHLYYT STKDHIIEED VRLPFAEVML GVLHLLRHKV LPRVNVVHYH LIATKMADPS DSTDFMLRST ATIDLTNPFT KEELARLRAL ATPLGMADEM DEYRCIDDLV MDIED
Validated NT Sequence
atcttactca cttaatcctc gatgtccatc acgaggtcat cgatgcagcg gtactcgtcc atctcatccg ccatcccgag cggggtcgcg agcgcccgca gtcgagcgag ttcctccttt gtaaacgggt tcgtcaagtc gatcgtcgct gtcgagcgca gcatgaagtc cgtgctgtct gacgggtctg ccatcttggt tgcgatgagg tggtagtgca ccacgttcac gcggggaagc accttgtggc gtaacaggtg caggacaccg agcatcacct ccgcaaaggg cagccgcacg tcttcctcga tgatgtggtc tttcgtagac gtgtagtaga ggtgcagcgt ggttccggaa aggcgaacga cgctgcagcc ggcgctctga tcgatcgctg ggcggaacgg ggagtagcgc ttctccttcg agttgaagaa catcttgtac gcctggacgg cggtgagcac ctttccaggt gtcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMT PGKVLTAVQA YKMFFNSKEK RYSPFRPAID QSAGCSVVRL SGTTLHLYYT STKDHIIEED VRLPFAEVML GVLHLLRHKV LPRVNVVHYH LIATKMADPS DSTDFMLRST ATIDLTNPFT KEELARLRAL ATPLGMADEM DEYRCIDDLV MDIED
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacac ctggaaaggt gctcaccgcc gtccaggcgt acaagatgtt cttcaactcg aaggagaagc gctactcccc gttccgccca gcgatcgatc agagcgccgg ctgcagcgtc gttcgccttt ccggaaccac gctgcacctc tactacacgt ctacgaaaga ccacatcatc gaggaagacg tgcggctgcc ctttgcggag gtgatgctcg gtgtcctgca cctgttacgc cacaaggtgc ttccccgcgt gaacgtggtg cactaccacc tcatcgcaac caagatggca gacccgtcag acagcacgga cttcatgctg cgctcgacag cgacgatcga cttgacgaac ccgtttacaa aggaggaact cgctcgactg cgggcgctcg cgaccccgct cgggatggcg gatgagatgg acgagtaccg ctgcatcgat gacctcgtga tggacatcga ggattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat