SSGCID: Target Page

LedoB.18889.a

alanyl-tRNA synthetase, putative

CENTER ID:	LedoB.18889.a
ORGANISM:	Leishmania donovani BPK282A1
ASSOCIATED DISEASE:	Leishmaniasis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LedoB.18889.a.B1.GE42683	full length		1	962

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LdBPK_221390.1
UniProt:	E9BG04

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSFTEWPVSR VRQEFVDYFK KQGHTFVPSS PVCPHDDPTL LFINAGMNQF KALFLGTADP NTDFGRLKRA ANSQMCIRAG GKHNDLEDVG RDTYHHTFFE MLGSWSFGDY FKKEAIQWAW ELLTEVYKLP KDQLYVTYFE GDENNGLDPD LEAKELWGQF LPESQIVPGN AKDNFWEMGD TGPCGPCSEV HFDRIGGRNA ASLVNKDDPM VVEIWNLVFM QFERRAGGSL TPLPQKHIDT GMGLERLTSI LQGVKSNYDT DAWIPLFEAI QTATGYPKSY AEIRNDPDSD ALVAYRVVAD HIRCLTSAVG DGVMPDSVGR GFVLRRIIRR AVRYGVQFLG AKPGFFTQLV DSVCTSLGPF FPHLKEPRNI QRIKTVLADE EQSFAKTWEM GLKHFNHAVN ECRANNSKVI SGSEAFVLHD RYGFPVDLTC LLAEKDGMTV DLEGFNAEMK ESKVSAGRVA ATKTFIDAYQ LEELKTRGIP QTDDAAKYVW EDSTGDLLAI FDKKNSKFIS LLEPGNDLGA EGVGIILSTT NFYAESGGQV YDTGRLVAAS DSVFEVKKVY NVAGYVVHVG NLSKDSASSI PSSAVVQLQV DYERRLPIAA NHTTTHQLNW VLRRVLGEKP DNFTEVQQKG SLVTDEMLRF DFSYNTKLSN EDLVRVERLL NEKIQAGLPV YREEVPLEAA SKINGLRHMF GEKYPDPVSV ISIGTPINEM LANPEKEEWR DYAVEFCGGT HLKNLKEAEK AVIISEEALM KGVRRVVVAT KSEADKVIKA GAVLKGQYDE LMKKEATATS VKALSVLNKK VGDSCIPLVL KNEMRDNIDA AIKRMNATLK SQAAEARDKA AEAGKALGAS YDAAAAPFLV HQMTEFGAER EALQAFSDGF TSTVSGPVGV FLIGSDDEKA LAIVSMPAAF VERKMSAVAW AKSSIGKGGG KPNAAQSGLP AKDVAAAAAK AMEEAERMKA SL

NT Sequence

atgtcgttca ccgagtggcc tgtgagtagg gttcgccagg agttcgtcga ctacttcaag aagcagggcc acacatttgt gcccagcagc cctgtctgcc cgcacgatga cccgaccctg ctgttcatca atgccggcat gaatcagttc aaggcgctct ttttggggac cgctgacccc aacactgact ttggccgtct gaagcgcgct gccaactcgc agatgtgcat tcgcgctggt ggcaagcaca acgacttgga ggatgtcggt cgcgacacgt accaccatac ctttttcgag atgctaggat catggtcctt tggcgactac ttcaagaaag aggccatcca gtgggcctgg gagctcctga cagaggtgta caagctgcct aaggatcagc tctacgtgac ctactttgag ggggacgaaa ataacggact ggacccggat ctggaggcga aggagctgtg gggccagttt ctcccagaga gccagatcgt ccccggcaac gccaaggaca acttttggga gatgggtgac actggcccgt gcggtccgtg ctcggaggtt cactttgacc gtatcggtgg acgcaacgct gctagcctcg tcaacaagga cgatccgatg gtggtggaga tctggaatct ggtgtttatg cagttcgagc gtcgcgctgg tggctccctc accccgctgc cgcagaagca catagacacc ggcatgggtc tggagcgcct cacttctatt ctgcagggtg tcaagtccaa ctatgacaca gatgcgtgga ttcctctctt tgaggccatc cagactgcga ccggctaccc caaatcgtac gcggagatcc gcaacgaccc cgacagcgat gccttggtgg cctaccgcgt tgtcgccgac cacattcgct gcctgacgtc tgccgtgggc gatggcgtca tgcccgactc cgtcggccgc ggcttcgtgc tgcgtcgaat tatccgccgc gctgtgcgct acggtgtgca gttcctcggt gccaagcctg gcttcttcac gcagctggtc gacagtgtgt gcacctcctt gggcccattc ttcccgcacc tgaaggaacc tcgcaatatc cagcgcatca agacggtgct ggcagacgag gagcagtcgt tcgccaagac atgggagatg gggctgaagc atttcaacca cgccgtgaat gagtgtcgag caaacaactc taaggtgatt agcggctccg aggcttttgt gcttcacgac cggtacgggt tcccggtgga tctgacgtgc ctgctggctg agaaggacgg catgacggtg gacctggagg gcttcaacgc ggagatgaag gagagcaagg ttagcgcagg tcgtgtggcg gccaccaaga ccttcattga cgcgtaccag ctcgaggagc tcaagacacg cggtattccg cagaccgatg acgccgcgaa gtatgtctgg gaggactcca ccggcgactt actcgctatc tttgacaaga agaacagcaa gttcatctct cttctggagc ctggcaacga ccttggagct gagggggtcg gtatcatcct gagcaccacg aacttttacg ccgagtctgg cgggcaggtg tacgacaccg gccgcctggt cgcggctagc gacagtgttt tcgaggtgaa gaaagtgtac aacgtggccg gctacgtggt gcatgtgggc aaccttagca aggactctgc ctcctcgatc ccctcgtccg cggtggtgca gctgcaggtg gactacgagc gccgcctgcc gattgcggca aaccatacca cgacgcacca gctgaactgg gtactgcgcc gtgtgctggg ggagaaaccg gacaacttca cagaggtgca gcagaagggc tccctcgtga cggacgagat gctccgcttc gatttcagct ataacacaaa gctgtcgaac gaggaccttg tgcgcgtgga gcggctactg aacgagaaga tccaggcggg cctgcccgtc taccgcgagg aggtgccgct ggaggctgct agcaagatca acggtcttcg ccatatgttt ggcgagaagt accccgaccc cgtcagcgtc atctccatcg gcaccccaat caacgaaatg ctggcgaacc ccgagaaaga ggagtggcgt gattacgccg tcgagttctg cggcggcacg cacctgaaaa acctcaagga agccgaaaag gcggtgataa tctcagagga ggccctcatg aagggcgtgc gccgcgtggt cgtggccacc aagtcggagg ctgacaaggt catcaaggct ggagctgttc tgaagggaca atacgatgag ctgatgaaaa aggaggcgac ggcgaccagc gtcaaggcgc tttcagtgct gaacaagaag gtaggtgaca gctgtattcc gctggtgctc aagaacgaga tgcgcgacaa catcgacgcg gccatcaagc ggatgaacgc gaccctcaag tcgcaggctg cagaggcaag ggataaagct gcagaagcag gcaaggccct cggtgcgtcg tatgacgctg ccgccgcgcc attcctcgta caccagatga ccgaattcgg cgcggagcgt gaggcactgc aggcgttttc cgacggcttc accagcaccg tgagcggacc ggtcggcgtc ttcctcatcg gcagcgacga cgagaaggcg ctcgcgattg tgtccatgcc ggccgctttt gtggagagga agatgagcgc tgtcgcgtgg gccaagtcct ccattggcaa gggtggcggt aagcccaacg ccgctcagtc cggcctcccc gccaaggacg tcgctgcggc tgcggcgaag gccatggaag aagcagagag gatgaaggcg tctctgtag

Details for LedoB.18889.a.B1.GE42683

HARVESTED ON:	9/28/2018
SEQUENCED ON:	10/17/2018
EXPECTED MW:	107kDa
OBSERVED MW:	107kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS FTEWPVSRVR QEFVDYFKKQ GHTFVPSSPV CPHDDPTLLF INAGMNQFKA LFLGTADPNT DFGRLKRAAN SQMCIRAGGK HNDLEDVGRD TYHHTFFEML GSWSFGDYFK KEAIQWAWEL LTEVYKLPKD QLYVTYFEGD ENNGLDPDLE AKELWGQFLP ESQIVPGNAK DNFWEMGDTG PCGPCSEVHF DRIGGRNAAS LVNKDDPMVV EIWNLVFMQF ERRAGGSLTP LPQKHIDTGM GLERLTSILQ GVKSNYDTDA WIPLFEAIQT ATGYPKSYAE IRNDPDSDAL VAYRVVADHI RCLTSAVGDG VMPDSVGRGF VLRRIIRRAV RYGVQFLGAK PGFFTQLVDS VCTSLGPFFP HLKEPRNIQR IKTVLADEEQ SFAKTWEMGL KHFNHAVNEC RANNSKVISG SEAFVLHDRY GFPVDLTCLL AEKDGMTVDL EGFNAEMKES KVSAGRVAAT KTFIDAYQLE ELKTRGIPQT DDAAKYVWED STGDLLAIFD KKNSKFISLL EPGNDLGAEG VGIILSTTNF YAESGGQVYD TGRLVAASDS VFEVKKVYNV AGYVVHVGNL SKDSASSIPS SAVVQLQVDY ERRLPIAANH TTTHQLNWVL RRVLGEKPDN FTEVQQKGSL VTDEMLRFDF SYNTKLSNED LVRVERLLNE KIQAGLPVYR EEVPLEAASK INGLRHMFGE KYPDPVSVIS IGTPINEMLA NPEKEEWRDY AVEFCGGTHL KNLKEAEKAV IISEEALMKG VRRVVVATKS EADKVIKAGA VLKGQYDELM KKEATATSVK ALSVLNKKVG DSCIPLVLKN EMRDNIDAAI KRMNATLKSQ AAEARDKAAE AGKALGASYD AAAAPFLVHQ MTEFGAEREA LQAFSDGFTS TVSGPVGVFL IGSDDEKALA IVSMPAAFVE RKMSAVAWAK SSIGKGGGKP NAAQSGLPAK DVAAAAAKAM EEAERMKASL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgt tcaccgagtg gcctgtgagt agggttcgcc aggagttcgt cgactacttc aagaagcagg gccacacatt tgtgcccagc agccctgtct gcccgcacga tgacccgacc ctgctgttca tcaatgccgg catgaatcag ttcaaggcgc tctttttggg gaccgctgac cccaacactg actttggccg tctgaagcgc gctgccaact cgcagatgtg cattcgcgct ggtggcaagc acaacgactt ggaggatgtc ggtcgcgaca cgtaccacca tacctttttc gagatgctag gatcatggtc ctttggcgac tacttcaaga aagaggccat ccagtgggcc tgggagctcc tgacagaggt gtacaagctg cctaaggatc agctctacgt gacctacttt gagggggacg aaaataacgg actggacccg gatctggagg cgaaggagct gtggggccag tttctcccag agagccagat cgtccccggc aacgccaagg acaacttttg ggagatgggt gacactggcc cgtgcggtcc gtgctcggag gttcactttg accgtatcgg tggacgcaac gctgctagcc tcgtcaacaa ggacgatccg atggtggtgg agatctggaa tctggtgttt atgcagttcg agcgtcgcgc tggtggctcc ctcaccccgc tgccgcagaa gcacatagac accggcatgg gtctggagcg cctcacttct attctgcagg gtgtcaagtc caactatgac acagatgcgt ggattcctct ctttgaggcc atccagactg cgaccggcta ccccaaatcg tacgcggaga tccgcaacga ccccgacagc gatgccttgg tggcctaccg cgttgtcgcc gaccacattc gctgcctgac gtctgccgtg ggcgatggcg tcatgcccga ctccgtcggc cgcggcttcg tgctgcgtcg aattatccgc cgcgctgtgc gctacggtgt gcagttcctc ggtgccaagc ctggcttctt cacgcagctg gtcgacagtg tgtgcacctc cttgggccca ttcttcccgc acctgaagga acctcgcaat atccagcgca tcaagacggt gctggcagac gaggagcagt cgttcgccaa gacatgggag atggggctga agcatttcaa ccacgccgtg aatgagtgtc gagcaaacaa ctctaaggtg attagcggct ccgaggcttt tgtgcttcac gaccggtacg ggttcccggt ggatctgacg tgcctgctgg ctgagaagga cggcatgacg gtggacctgg agggcttcaa cgcggagatg aaggagagca aggttagcgc aggtcgtgtg gcggccacca agaccttcat tgacgcgtac cagctcgagg agctcaagac acgcggtatt ccgcagaccg atgacgccgc gaagtatgtc tgggaggact ccaccggcga cttactcgct atctttgaca agaagaacag caagttcatc tctcttctgg agcctggcaa cgaccttgga gctgaggggg tcggtatcat cctgagcacc acgaactttt acgccgagtc tggcgggcag gtgtacgaca ccggccgcct ggtcgcggct agcgacagtg ttttcgaggt gaagaaagtg tacaacgtgg ccggctacgt ggtgcatgtg ggcaacctta gcaaggactc tgcctcctcg atcccctcgt ccgcggtggt gcagctgcag gtggactacg agcgccgcct gccgattgcg gcaaaccata ccacgacgca ccagctgaac tgggtactgc gccgtgtgct gggggagaaa ccggacaact tcacagaggt gcagcagaag ggctccctcg tgacggacga gatgctccgc ttcgatttca gctataacac aaagctgtcg aacgaggacc ttgtgcgcgt ggagcggcta ctgaacgaga agatccaggc gggcctgccc gtctaccgcg aggaggtgcc gctggaggct gctagcaaga tcaacggtct tcgccatatg tttggcgaga agtaccccga ccccgtcagc gtcatctcca tcggcacccc aatcaacgaa atgctggcga accccgagaa agaggagtgg cgtgattacg ccgtcgagtt ctgcggcggc acgcacctga aaaacctcaa ggaagccgaa aaggcggtga taatctcaga ggaggccctc atgaagggcg tgcgccgcgt ggtcgtggcc accaagtcgg aggctgacaa ggtcatcaag gctggagctg ttctgaaggg acaatacgat gagctgatga aaaaggaggc gacggcgacc agcgtcaagg cgctttcagt gctgaacaag aaggtaggtg acagctgtat tccgctggtg ctcaagaacg agatgcgcga caacatcgac gcggccatca agcggatgaa cgcgaccctc aagtcgcagg ctgcagaggc aagggataaa gctgcagaag caggcaaggc cctcggtgcg tcgtatgacg ctgccgccgc gccattcctc gtacaccaga tgaccgaatt cggcgcggag cgtgaggcac tgcaggcgtt ttccgacggc ttcaccagca ccgtgagcgg accggtcggc gtcttcctca tcggcagcga cgacgagaag gcgctcgcga ttgtgtccat gccggccgct tttgtggaga ggaagatgag cgctgtcgcg tgggccaagt cctccattgg caagggtggc ggtaagccca acgccgctca gtccggcctc cccgccaagg acgtcgctgc ggctgcggcg aaggccatgg aagaagcaga gaggatgaag gcgtctctgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat