LedoB.18856.a

selenocysteine tRNA synthase; O-phosphoseryl-tRNA selenium transferase

CENTER ID: LedoB.18856.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.18856.a.B1.GE39094 full length 1 595
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:LdBPK_091000.1
RefSeq: XP_003858779.1
UniProt: E9B9Y6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDDRSLKLAE DFVSARYIEA GRESLRATAR IMRSILAQRC CPDEGLTDAA IELILRQLSL MDTNNLAHHV GGGEREGRVV SALVRMRHFH LTHGIGRSGD LFSEQPKAAG SSLLYKITNV LMLDLIRQAG APSTAAAVVV PMATGMTLAL VLRCVAKTHM KELMKEAEAV QLQRTVTKDS TSATSAAPVQ EPPMSEADRD RHDRTSLPVP ATPRYVIWPR IDQKTALKCI DAAGLVPVPV QLRPAVPLAR SAAPCVSTNR DSLDRGQDSI GSPSTPTSSS SLFLECHVDD VAAAVNAVGG PSQVVCVLST TSCFAPRLPD NTVAIAQYCK KAGIPYVVNN AYGVQSRRIM TRLDAAQRLG RVDFVVQSGD KNFLVPVGGS IICSGDKERC KAVAALYAGR ASMSPIVDLF ITALSLGRRG MQTLWSDRYK CRARLIRQLR VFARERREVL LVDDSDDDKA DEDTVGGSQR TSNAVVPRND ISVAVTMRAY GLPAAEASSS GAQLGSEQAG RVTNWAAARA LGAQLFRSAV TGPRVITPAP STPTTIAGCT FRNYGMHQDR EPPCPLLVIA CGIGMSESEV DALMARLRDL WPVPA
NT Sequence
atggatgatc gctcgctaaa actggcggag gacttcgtct cggcgcggta catcgaagcg ggccgcgagt cgttgcgtgc aacggcccgt atcatgcgct ccatcctggc gcagcgctgc tgcccagatg aaggtctcac cgatgccgcc atcgagctca tccttcgtca gctttcgctc atggacacaa acaacttagc gcaccacgtc ggtggcggcg agcgcgaggg ccgcgtcgtc tctgccctcg ttcgcatgcg acacttccac ctcacgcacg gcattgggcg ctccggcgac ttgttcagcg aacagccgaa agcggccggg tcgtcactgc tgtacaagat aacgaacgtg ctcatgcttg atctgatacg ccaggccggt gccccctcga ccgcggccgc ggtggtggtg ccgatggcga cgggcatgac gctggcgctg gtgcttcggt gtgtggcgaa gacgcacatg aaagaactga tgaaggaggc agaagcggtg cagctgcaac gcactgtgac gaaggactca acgagcgcaa cgtcagctgc cccagtgcaa gagccgccaa tgagcgaggc tgatcgtgat aggcatgacc gcacatctct gccagtcccg gcgacgccgc gctacgtcat atggcctcgc atcgatcaga agacagccct caagtgtatt gacgcggcgg ggctagtgcc ggtgccggta cagctacggc cggctgtgcc gctcgcacgc tctgccgccc cttgcgtcag caccaacagg gatagcctcg accgtggcca agacagcatt ggttcgccgt cgacgccaac gtcctcgtcg tcgctgttcc tcgagtgcca cgtggacgac gtagccgcgg ccgtgaacgc cgttggcggc ccgtcacagg tggtctgtgt gctctccacg accagctgct tcgcgccacg cctgccagat aacacggtcg ccattgcgca gtactgcaag aaggccggca ttccctatgt ggtgaacaac gcgtacgggg tgcagagccg ccgcattatg actcgcctcg acgcggcgca gcgactgggg cgggtggact ttgtggttca gtccggtgac aagaactttc tcgtcccggt gggcggctcc atcatctgct ccggcgacaa ggagcgctgc aaggccgtgg cagccctcta cgccggtcgc gcgagcatga gccccatcgt cgacctcttc atcaccgcct tgagcctcgg ccggcgcggc atgcagacct tgtggagcga tcgctacaag tgccgtgcgc ggctgatccg gcagctgcgc gtgttcgcgc gcgagcgaag ggaagtgctg ctggtcgacg acagtgacga cgacaaggcc gatgaggata ccgtaggtgg gtcgcaaagg acctccaatg ctgtcgtgcc ccgcaatgac atcagtgtcg ccgtcaccat gcgtgcctac ggccttcctg ccgccgaagc gagcagcagt ggcgcccaac ttgggtcgga gcaggcgggg agggtgacaa actgggcggc cgcccgcgct ctcggcgcac aactctttcg cagcgcggtc accggccccc gcgtcatcac tccagcaccg tccaccccaa ccacaattgc cggctgcaca ttcaggaatt acggcatgca tcaagacagg gagccgccgt gcccgctgct ggtgatcgcc tgcggcatcg gcatgtcgga gtctgaagtc gatgctctga tggcgcgcct tcgcgacctg tggcctgttc ccgcatga
Details for LedoB.18856.a.B1.GE39094
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 65kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 90
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMD DRSLKLAEDF VSARYIEAGR ESLRATARIM RSILAQRCCP DEGLTDAAIE LILRQLSLMD TNNLAHHVGG GEREGRVVSA LVRMRHFHLT HGIGRSGDLF SEQPKAAGSS LLYKITNVLM LDLIRQAGAP STAAAVVVPM ATGMTLALVL RCVAKTHMKE LMKEAEAVQL QRTVTKDSTS ATSAAPVQEP PMSEADRDRH DRTSLPVPAT PRYVIWPRID QKTALKCIDA AGLVPVPVQL XXAVPLARSA APCVXTXXDS XDRXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXV TGXXVCSPRP AXXXPRLPDN TXAIAQYCKK AGIPYVVNNA YGVQSRRIMT RLDAAQRLGR VDFVVQSGDK NFLVPVGGSI ICSGDKERCK AVAALYAGRA SMSPIVDLFI TALSLGRRGM QTLWSDRYKC RARLIRQLRV FARERREVLL VDDSDDDKAD EDTVGGSQRT SNAVVPRNDI SVAVTMRAYG LPAAEASSSG AQLGSEQAGR VTNWAAARAL GAQLFRSAVT GPRVITPAPS TLTTIAGCTF RNYGMHQDRE PPCPLLVIAC GIGMSESEVD ALMARL
Validated NT Sequence
gcgaaggcgc gccatcagag catcgacttc agactccgac atgccgatgc cgcaggcgat caccagcagc gggcacggcg gctccctgtc ttgatgcatg ccgtaattcc tgaatgtgca gccggcaatt gtggttaggg tggacggtgc tggagtgatg acgcgggggc cggtgaccgc gctgcgaaag agttgtgcgc cgagagcgcg ggcggccgcc cagtttgtca ccctccccgc ctgctccgac ccaagttggg cgccactgct gctcgcttcg gcggcaggaa ggccgtaggc acgcatggtg acggcgacac tgatgtcatt gcggggcacg acagcattgg aggtcctttg cgacccacct acggtatcct catcggcctt gtcgtcgtca ctgtcgtcga ccagcagcac ttcccttcgc tcgcgcgcga acacgcgcag ctgccggatc agccgcgcac ggcacttgta gcgatcgctc cacaaggtct gcatgccgcg ccggccgagg ctcaaggcgg tgatgaagag gtcgacgatg gggctcatgc tcgcgcgacc ggcgtagagg gctgccacgg ccttgcagcg ctccttgtcg ccggagcaga tgatggagcc gcccaccggg acgagaaagt tcttgtcacc ggactggacc acaaagtcca cccgccccag tcgctgcgcc gcgtcgaggc gagtcataat gcggcggctc tgcaccccgt acgcgttgtt caccacatag ggaatgccgg ccttcttgca gtactgggca atggcgnccg tgttatctgg taggcgtggc gnnnnnnnag ctggtcgtgg agagcacacn nnnnnncctg tgacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctnnangaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacggtcnn ngctatccnn nntggtgcng acgcangggg cggcagagcg tgcgagcggc acagccgnnn ntagctgtac cggcaccggc actagccccg ccgcgtcaat acacttgagg gctgtcttct gatcgatgcg aggccatatg acgtagcgcg gcgtcgccgg gactggcaga gatgtgcggt catgcctatc acgatcagcc tcgctcattg gcggctcttg cactggggca gctgacgttg cgctcgttga gtccttcgtc acagtgcgtt gcagctgcac cgcttctgcc tccttcatca gttctttcat gtgcgtcttc gccacacacc gaagcaccag cgccagcgtc atgcccgtcg ccatcggcac caccaccgcg gccgcggtcg agggggcacc ggcctggcgt atcagatcaa gcatgagcac gttcgttatc ttgtacagca gtgacgaccc ggccgctttc ggctgttcgc tgaacaagtc gccggagcgc ccaatgccgt gcgtgaggtg gaagtgtcgc atgcgaacga gggcagagac gacgcggccc tcgcgctcgc cgccaccgac gtggtgcgct aagttgtttg tgtccatgag cgaaagctga cgaaggatga gctcgatggc ggcatcggtg agaccttcat ctgggcagca gcgctgcgcc aggatggagc gcatgatacg ggccgttgca cgcaacgact cgcggcccgc ttcgatgtac cgcgccgaga cgaagtcctc cgccagtttt agcgagcgat catccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMD DRSLKLAEDF VSARYIEAGR ESLRATARIM RSILAQRCCP DEGLTDAAIE LILRQLSLMD TNNLAHHVGG GEREGRVVSA LVRMRHFHLT HGIGRSGDLF SEQPKAAGSS LLYKITNVLM LDLIRQAGAP STAAAVVVPM ATGMTLALVL RCVAKTHMKE LMKEAEAVQL QRTVTKDSTS ATSAAPVQEP PMSEADRDRH DRTSLPVPAT PRYVIWPRID QKTALKCIDA AGLVPVPVQL RPAVPLARSA APCVSTNRDS LDRGQDSIGS PSTPTSSSSL FLECHVDDVA AAVNAVGGPS QVVCVLSTTS CFAPRLPDNT VAIAQYCKKA GIPYVVNNAY GVQSRRIMTR LDAAQRLGRV DFVVQSGDKN FLVPVGGSII CSGDKERCKA VAALYAGRAS MSPIVDLFIT ALSLGRRGMQ TLWSDRYKCR ARLIRQLRVF ARERREVLLV DDSDDDKADE DTVGGSQRTS NAVVPRNDIS VAVTMRAYGL PAAEASSSGA QLGSEQAGRV TNWAAARALG AQLFRSAVTG PRVITPAPST PTTIAGCTFR NYGMHQDREP PCPLLVIACG IGMSESEVDA LMARLRDLWP VPA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatg atcgctcgct aaaactggcg gaggacttcg tctcggcgcg gtacatcgaa gcgggccgcg agtcgttgcg tgcaacggcc cgtatcatgc gctccatcct ggcgcagcgc tgctgcccag atgaaggtct caccgatgcc gccatcgagc tcatccttcg tcagctttcg ctcatggaca caaacaactt agcgcaccac gtcggtggcg gcgagcgcga gggccgcgtc gtctctgccc tcgttcgcat gcgacacttc cacctcacgc acggcattgg gcgctccggc gacttgttca gcgaacagcc gaaagcggcc gggtcgtcac tgctgtacaa gataacgaac gtgctcatgc ttgatctgat acgccaggcc ggtgccccct cgaccgcggc cgcggtggtg gtgccgatgg cgacgggcat gacgctggcg ctggtgcttc ggtgtgtggc gaagacgcac atgaaagaac tgatgaagga ggcagaagcg gtgcagctgc aacgcactgt gacgaaggac tcaacgagcg caacgtcagc tgccccagtg caagagccgc caatgagcga ggctgatcgt gataggcatg accgcacatc tctgccagtc ccggcgacgc cgcgctacgt catatggcct cgcatcgatc agaagacagc cctcaagtgt attgacgcgg cggggctagt gccggtgccg gtacagctac ggccggctgt gccgctcgca cgctctgccg ccccttgcgt cagcaccaac agggatagcc tcgaccgtgg ccaagacagc attggttcgc cgtcgacgcc aacgtcctcg tcgtcgctgt tcctcgagtg ccacgtggac gacgtagccg cggccgtgaa cgccgttggc ggcccgtcac aggtggtctg tgtgctctcc acgaccagct gcttcgcgcc acgcctgcca gataacacgg tcgccattgc gcagtactgc aagaaggccg gcattcccta tgtggtgaac aacgcgtacg gggtgcagag ccgccgcatt atgactcgcc tcgacgcggc gcagcgactg gggcgggtgg actttgtggt tcagtccggt gacaagaact ttctcgtccc ggtgggcggc tccatcatct gctccggcga caaggagcgc tgcaaggccg tggcagccct ctacgccggt cgcgcgagca tgagccccat cgtcgacctc ttcatcaccg ccttgagcct cggccggcgc ggcatgcaga ccttgtggag cgatcgctac aagtgccgtg cgcggctgat ccggcagctg cgcgtgttcg cgcgcgagcg aagggaagtg ctgctggtcg acgacagtga cgacgacaag gccgatgagg ataccgtagg tgggtcgcaa aggacctcca atgctgtcgt gccccgcaat gacatcagtg tcgccgtcac catgcgtgcc tacggccttc ctgccgccga agcgagcagc agtggcgccc aacttgggtc ggagcaggcg gggagggtga caaactgggc ggccgcccgc gctctcggcg cacaactctt tcgcagcgcg gtcaccggcc cccgcgtcat cactccagca ccgtccaccc caaccacaat tgccggctgc acattcagga attacggcat gcatcaagac agggagccgc cgtgcccgct gctggtgatc gcctgcggca tcggcatgtc ggagtctgaa gtcgatgctc tgatggcgcg ccttcgcgac ctgtggcctg ttcccgcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat