LedoB.18849.a

valyl-tRNA synthetase, putative

CENTER ID: LedoB.18849.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.18849.a.B1.GE42682 full length 1 967
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.18849.a.B1.PW38610 full length 1 967

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LdBPK_303170.1
UniProt: E9BM60

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAATYDPAAV EADWYPWWEK SGFFRPASDH KSETATKPFV IIAPPPNVTG YLHLGHALTG AVQDTLIRFH RMKGDNTLYL PGTDHAGIAT QVVVERRVMK EEGKSRHDLG RDEFMKRLWE FKKNHAGMIT EQFRRIGLSL DWTRERFTMD EQSSAAVVEA FVRLHEDGLV HRDTRLVNWC CALQSAISDL EVEFVEVPKT SKMTIPLYDR KVDMGSLTHV AYKLADSDDE LVIATTRPET LLGDTAVAIH PDDERYKKFH GKFLKCPFRD DIIPIVLDAT LVDMNFGTGA VKITPAHDPN DFESGKRHNL QQLVMMDLKG YVTMEPFKGM HRFDCRREIV KKLEEMGLLR GVEPYEYRVG RCERTGDIVE PMLMPQWFID CSDMARRSVE AVRNGDLRLY PPSHEAVWYH WLENIKPWCV SRQLWWGHRI PAYKVVGSLP KDVDPWVVAR NLQEAHAKAK KKFGLTDEQV AEASFEQDPD VLDTWFSSGL WPFATLGWPT DSDDMKRFFP NSLMETGHDI LFFWVARMVM LSLHFTNKLP YKEVFLHAMV RDKNGEKMSK SKGNVIDPLY VIHGVSLQTL HNTVRSGNLG DKEVEKAIKQ QKEFFPEGIP ECGSDALRFG LLSYTQSGRS VNLDIQRVVA YRQFCNKLWN VVRYVLYHAL GTDYVPSKQQ FSPAEDAAML PLECRWILSR LDAAIAEATQ GMSEGLYDFA LATGAVYRFW LHELCDVFLE LTKPTIQKGG EKQQLVQDVL LHVVEKALRL LHPMMPFLTE ELWHRLPNYS SFGSESIMLA KYPTPSGWLS AASDSAMSII LDVVHSVRST KASYSLTNKH KPEVWVTAHT AELQELFAAE KMMISTLGVV GEVTVVSPAE EAVAVPKGCG FSVVTKEVGV NMMLMGFIDV GKEVAKLEKQ LDGLMKQIEG MKKKMSIPNY ETKVPAEIRV TNTEKLKTLE AQSAQLRDGI EKMSSLK
NT Sequence
atggcggcca cgtacgatcc ggccgcggtg gaggcggact ggtatccgtg gtgggagaag tctggcttct tcagacccgc ctcagaccac aagtccgaga cggcgaccaa gccgttcgtg attatcgccc caccaccgaa cgtcacgggc tacctgcacc tgggccatgc cctcacgggc gctgtgcagg acaccctcat ccgcttccac cgcatgaagg gtgacaacac cctctacttg cccggcaccg accacgccgg catcgccacc caggtcgtcg tggagcgccg cgtcatgaag gaggagggca agagccggca cgacctcggc cgcgacgagt tcatgaagcg cctgtgggaa ttcaagaaga atcacgctgg catgatcacg gagcagttcc gcaggatcgg cttgtcgctg gactggacgc gcgagcgctt tacgatggac gaacagtcgt ctgcggctgt tgtggaggcg tttgtgcgcc tgcacgagga cggcctggtg caccgcgaca cacggctggt aaactggtgc tgcgcgctgc agagcgccat ctctgacctc gaggtggagt ttgtcgaagt gcccaagaca tccaagatga ccattcctct ctatgaccgc aaagtcgaca tgggcagcct cactcacgtt gcctacaagc tggcggacag cgacgacgag ctggtgattg cgacgacgcg tcctgagacg ctgctgggcg atacggccgt cgccattcac cctgacgacg agcgctacaa gaagttccat ggcaagttcc tcaagtgccc gttccgcgac gacatcatcc cgatcgtgct ggacgcgacg ctggtcgaca tgaacttcgg caccggcgcg gtgaaaatca ccccagctca cgacccaaac gactttgagt ccggcaagcg ccacaacctg cagcagcttg tcatgatgga cctgaagggc tacgtgacga tggagccgtt caagggcatg caccgcttcg actgccgccg cgagatcgtg aagaagctgg aggagatggg gctgctgcgc ggcgtggagc cgtacgagta ccgcgtaggc cgctgcgagc gcactggcga cattgtggag ccgatgctga tgccgcagtg gttcatcgac tgctccgata tggcccgccg ctccgtcgag gcggtgcgca acggcgacct gcgcctgtac ccgccttcgc acgaggccgt ctggtaccac tggctggaga acatcaaacc gtggtgcgtg tcgcgtcagc tctggtgggg ccaccgcatt cctgcctaca aagttgtcgg ctcgctgccg aaagacgtgg acccatgggt ggtcgcccgc aatctgcagg aggcgcacgc gaaggctaag aagaagttcg gcctgactga cgagcaggtc gccgaggcct cgtttgagca ggacccggat gtgctggaca cctggttcag ctccggactc tggcccttcg ccacgcttgg atggcccacc gactcggacg acatgaagcg cttcttcccg aactcgctga tggagaccgg ccacgacatc ctgttcttct gggtggctcg catggtaatg ctttcgctgc acttcacaaa caagctgccg tacaaggagg tgtttctgca cgcaatggtg cgcgacaaga atggcgagaa gatgtccaag tcgaagggca acgtcatcga cccactctac gtcatccacg gcgtttcgct gcagaccctc cacaacaccg tgcgcagcgg caacctgggc gacaaggagg tggagaaggc catcaagcag cagaaggagt ttttcccaga aggcattcca gagtgcggta gcgatgccct gcgcttcggc ctcctctcct acacccagtc tggccgcagc gtgaacctcg acatccaacg cgtcgtggcc taccgccagt tctgcaacaa gctgtggaac gtggtgcgct acgtgctgta tcacgccctg ggcactgact acgtgcccag caagcagcag ttcagccccg ccgaggacgc cgccatgctg ccgctcgagt gccgctggat tctctcgcgc ctcgacgccg ccatcgcgga ggccacgcag ggcatgtcgg agggcctcta cgacttcgcg ttggccaccg gtgccgtcta ccgcttctgg ctgcacgagc tgtgcgacgt gttccttgag ttgacgaagc cgaccattca gaagggcgga gagaagcagc agctggtgca agacgtgctg ctgcacgtcg tcgagaaggc gcttcggctg ctacacccga tgatgccgtt tctcacggag gagctttggc accgcctgcc caactacagc agcttcggca gcgagtcgat catgctcgcc aagtacccga caccgagcgg gtggttgagc gcggcgtcgg atagcgccat gtcgatcatt ctcgacgtcg tgcacagcgt acgttccacc aaggcctcgt actcgctaac gaacaagcac aagccagagg tgtgggtgac ggcgcatacg gcggagctgc aggagctgtt cgcggcggaa aagatgatga tctcaaccct cggtgtcgtg ggcgaggtga ctgtcgtgtc cccggccgag gaggcagtgg cggtgccgaa gggctgcggc ttctccgtgg tgacaaagga ggtgggcgtg aacatgatgc tcatgggttt catcgacgtc gggaaggagg tggcgaagct ggagaagcag ctggacggcc tcatgaagca gattgaggga atgaagaaga agatgtctat ccctaattac gaaactaagg tgccggccga gatccgcgtg accaacacgg agaagctcaa gacgctagag gcgcagagcg cgcagctgcg cgatggcatc gagaagatga gcagcctcaa gtga
Details for LedoB.18849.a.B1.GE42682
HARVESTED ON: 9/28/2018
SEQUENCED ON: 10/17/2018
EXPECTED MW: 111kDa
OBSERVED MW: 111kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA ATYDPAAVEA DWYPWWEKSG FFRPASDHKS ETATKPFVII APPPNVTGYL HLGHALTGAV QDTLIRFHRM KGDNTLYLPG TDHAGIATQV VVERRVMKEE GKSRHDLGRD EFMKRLWEFK KNHAGMITEQ FRRIGLSLDW TRERFTMDEQ SSAAVVEAFV RLHEDGLVHR DTRLVNWCCA LQSAISDLEV EFVEVPKTSK MTIPLYDRKV DMGSLTHVAY KLADSDDELV IATTRPETLL GDTAVAIHPD DERYKKFHGK FLKCPFRDDI IPIVLDATLV DMNFGTGAVK ITPAHDPNDF ESGKRHNLQQ LVMMDLKGYV TMEPFKGMHR FDCRREIVKK LEEMGLLRGV EPYEYRVGRC ERTGDIVEPM LMPQWFIDCS DMARRSVEAV RNGDLRLYPP SHEAVWYHWL ENIKPWCVSR QLWWGHRIPA YKVVGSLPKD VDPWVVARNL QEAHAKAKKK FGLTDEQVAE ASFEQDPDVL DTWFSSGLWP FATLGWPTDS DDMKRFFPNS LMETGHDILF FWVARMVMLS LHFTNKLPYK EVFLHAMVRD KNGEKMSKSK GNVIDPLYVI HGVSLQTLHN TVRSGNLGDK EVEKAIKQQK EFFPEGIPEC GSDALRFGLL SYTQSGRSVN LDIQRVVAYR QFCNKLWNVV RYVLYHALGT DYVPSKQQFS PAEDAAMLPL ECRWILSRLD AAIAEATQGM SEGLYDFALA TGAVYRFWLH ELCDVFLELT KPTIQKGGEK QQLVQDVLLH VVEKALRLLH PMMPFLTEEL WHRLPNYSSF GSESIMLAKY PTPSGWLSAA SDSAMSIILD VVHSVRSTKA SYSLTNKHKP EVWVTAHTAE LQELFAAEKM MISTLGVVGE VTVVSPAEEA VAVPKGCGFS VVTKEVGVNM MLMGFIDVGK EVAKLEKQLD GLMKQIEGMK KKMSIPNYET KVPAEIRVTN TEKLKTLEAQ SAQLRDGIEK MSSLK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg ccacgtacga tccggccgcg gtggaggcgg actggtatcc gtggtgggag aagtctggct tcttcagacc cgcctcagac cacaagtccg agacggcgac caagccgttc gtgattatcg ccccaccacc gaacgtcacg ggctacctgc acctgggcca tgccctcacg ggcgctgtgc aggacaccct catccgcttc caccgcatga agggtgacaa caccctctac ttgcccggca ccgaccacgc cggcatcgcc acccaggtcg tcgtggagcg ccgcgtcatg aaggaggagg gcaagagccg gcacgacctc ggccgcgacg agttcatgaa gcgcctgtgg gaattcaaga agaatcacgc tggcatgatc acggagcagt tccgcaggat cggcttgtcg ctggactgga cgcgcgagcg ctttacgatg gacgaacagt cgtctgcggc tgttgtggag gcgtttgtgc gcctgcacga ggacggcctg gtgcaccgcg acacacggct ggtaaactgg tgctgcgcgc tgcagagcgc catctctgac ctcgaggtgg agtttgtcga agtgcccaag acatccaaga tgaccattcc tctctatgac cgcaaagtcg acatgggcag cctcactcac gttgcctaca agctggcgga cagcgacgac gagctggtga ttgcgacgac gcgtcctgag acgctgctgg gcgatacggc cgtcgccatt caccctgacg acgagcgcta caagaagttc catggcaagt tcctcaagtg cccgttccgc gacgacatca tcccgatcgt gctggacgcg acgctggtcg acatgaactt cggcaccggc gcggtgaaaa tcaccccagc tcacgaccca aacgactttg agtccggcaa gcgccacaac ctgcagcagc ttgtcatgat ggacctgaag ggctacgtga cgatggagcc gttcaagggc atgcaccgct tcgactgccg ccgcgagatc gtgaagaagc tggaggagat ggggctgctg cgcggcgtgg agccgtacga gtaccgcgta ggccgctgcg agcgcactgg cgacattgtg gagccgatgc tgatgccgca gtggttcatc gactgctccg atatggcccg ccgctccgtc gaggcggtgc gcaacggcga cctgcgcctg tacccgcctt cgcacgaggc cgtctggtac cactggctgg agaacatcaa accgtggtgc gtgtcgcgtc agctctggtg gggccaccgc attcctgcct acaaagttgt cggctcgctg ccgaaagacg tggacccatg ggtggtcgcc cgcaatctgc aggaggcgca cgcgaaggct aagaagaagt tcggcctgac tgacgagcag gtcgccgagg cctcgtttga gcaggacccg gatgtgctgg acacctggtt cagctccgga ctctggccct tcgccacgct tggatggccc accgactcgg acgacatgaa gcgcttcttc ccgaactcgc tgatggagac cggccacgac atcctgttct tctgggtggc tcgcatggta atgctttcgc tgcacttcac aaacaagctg ccgtacaagg aggtgtttct gcacgcaatg gtgcgcgaca agaatggcga gaagatgtcc aagtcgaagg gcaacgtcat cgacccactc tacgtcatcc acggcgtttc gctgcagacc ctccacaaca ccgtgcgcag cggcaacctg ggcgacaagg aggtggagaa ggccatcaag cagcagaagg agtttttccc agaaggcatt ccagagtgcg gtagcgatgc cctgcgcttc ggcctcctct cctacaccca gtctggccgc agcgtgaacc tcgacatcca acgcgtcgtg gcctaccgcc agttctgcaa caagctgtgg aacgtggtgc gctacgtgct gtatcacgcc ctgggcactg actacgtgcc cagcaagcag cagttcagcc ccgccgagga cgccgccatg ctgccgctcg agtgccgctg gattctctcg cgcctcgacg ccgccatcgc ggaggccacg cagggcatgt cggagggcct ctacgacttc gcgttggcca ccggtgccgt ctaccgcttc tggctgcacg agctgtgcga cgtgttcctt gagttgacga agccgaccat tcagaagggc ggagagaagc agcagctggt gcaagacgtg ctgctgcacg tcgtcgagaa ggcgcttcgg ctgctacacc cgatgatgcc gtttctcacg gaggagcttt ggcaccgcct gcccaactac agcagcttcg gcagcgagtc gatcatgctc gccaagtacc cgacaccgag cgggtggttg agcgcggcgt cggatagcgc catgtcgatc attctcgacg tcgtgcacag cgtacgttcc accaaggcct cgtactcgct aacgaacaag cacaagccag aggtgtgggt gacggcgcat acggcggagc tgcaggagct gttcgcggcg gaaaagatga tgatctcaac cctcggtgtc gtgggcgagg tgactgtcgt gtccccggcc gaggaggcag tggcggtgcc gaagggctgc ggcttctccg tggtgacaaa ggaggtgggc gtgaacatga tgctcatggg tttcatcgac gtcgggaagg aggtggcgaa gctggagaag cagctggacg gcctcatgaa gcagattgag ggaatgaaga agaagatgtc tatccctaat tacgaaacta aggtgccggc cgagatccgc gtgaccaaca cggagaagct caagacgcta gaggcgcaga gcgcgcagct gcgcgatggc atcgagaaga tgagcagcct caagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for LedoB.18849.a.B1.PW38610
PURIFICATION DATe: 5/1/2019
CONCENTRATION: 12.8mg/ml
OBSERVED MW: 120kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1 mM Valine, 1 mM ATP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 55µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMA ATYDPAAVEA DWYPWWEKSG FFRPASDHKS ETATKPFVII APPPNVTGYL HLGHALTGAV QDTLIRFHRM KGDNTLYLPG TDHAGIATQV VVERRVMKEE GKSRHDLGRD EFMKRLWEFK KNHAGMITEQ FRRIGLSLDW TRERFTMDEQ SSAAVVEAFV RLHEDGLVHR DTRLVNWCCA LQSAISDLEV EFVEVPKTSK MTIPLYDRKV DMGSLTHVAY KLADSDDELV IATTRPETLL GDTAVAIHPD DERYKKFHGK FLKCPFRDDI IPIVLDATLV DMNFGTGAVK ITPAHDPNDF ESGKRHNLQQ LVMMDLKGYV TMEPFKGMHR FDCRREIVKK LEEMGLLRGV EPYEYRVGRC ERTGDIVEPM LMPQWFIDCS DMARRSVEAV RNGDLRLYPP SHEAVWYHWL ENIKPWCVSR QLWWGHRIPA YKVVGSLPKD VDPWVVARNL QEAHAKAKKK FGLTDEQVAE ASFEQDPDVL DTWFSSGLWP FATLGWPTDS DDMKRFFPNS LMETGHDILF FWVARMVMLS LHFTNKLPYK EVFLHAMVRD KNGEKMSKSK GNVIDPLYVI HGVSLQTLHN TVRSGNLGDK EVEKAIKQQK EFFPEGIPEC GSDALRFGLL SYTQSGRSVN LDIQRVVAYR QFCNKLWNVV RYVLYHALGT DYVPSKQQFS PAEDAAMLPL ECRWILSRLD AAIAEATQGM SEGLYDFALA TGAVYRFWLH ELCDVFLELT KPTIQKGGEK QQLVQDVLLH VVEKALRLLH PMMPFLTEEL WHRLPNYSSF GSESIMLAKY PTPSGWLSAA SDSAMSIILD VVHSVRSTKA SYSLTNKHKP EVWVTAHTAE LQELFAAEKM MISTLGVVGE VTVVSPAEEA VAVPKGCGFS VVTKEVGVNM MLMGFIDVGK EVAKLEKQLD GLMKQIEGMK KKMSIPNYET KVPAEIRVTN TEKLKTLEAQ SAQLRDGIEK MSSLK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGG CCACGTACGA TCCGGCCGCG GTGGAGGCGG ACTGGTATCC GTGGTGGGAG AAGTCTGGCT TCTTCAGACC CGCCTCAGAC CACAAGTCCG AGACGGCGAC CAAGCCGTTC GTGATTATCG CCCCACCACC GAACGTCACG GGCTACCTGC ACCTGGGCCA TGCCCTCACG GGCGCTGTGC AGGACACCCT CATCCGCTTC CACCGCATGA AGGGTGACAA CACCCTCTAC TTGCCCGGCA CCGACCACGC CGGCATCGCC ACCCAGGTCG TCGTGGAGCG CCGCGTCATG AAGGAGGAGG GCAAGAGCCG GCACGACCTC GGCCGCGACG AGTTCATGAA GCGCCTGTGG GAATTCAAGA AGAATCACGC TGGCATGATC ACGGAGCAGT TCCGCAGGAT CGGCTTGTCG CTGGACTGGA CGCGCGAGCG CTTTACGATG GACGAACAGT CGTCTGCGGC TGTTGTGGAG GCGTTTGTGC GCCTGCACGA GGACGGCCTG GTGCACCGCG ACACACGGCT GGTAAACTGG TGCTGCGCGC TGCAGAGCGC CATCTCTGAC CTCGAGGTGG AGTTTGTCGA AGTGCCCAAG ACATCCAAGA TGACCATTCC TCTCTATGAC CGCAAAGTCG ACATGGGCAG CCTCACTCAC GTTGCCTACA AGCTGGCGGA CAGCGACGAC GAGCTGGTGA TTGCGACGAC GCGTCCTGAG ACGCTGCTGG GCGATACGGC CGTCGCCATT CACCCTGACG ACGAGCGCTA CAAGAAGTTC CATGGCAAGT TCCTCAAGTG CCCGTTCCGC GACGACATCA TCCCGATCGT GCTGGACGCG ACGCTGGTCG ACATGAACTT CGGCACCGGC GCGGTGAAAA TCACCCCAGC TCACGACCCA AACGACTTTG AGTCCGGCAA GCGCCACAAC CTGCAGCAGC TTGTCATGAT GGACCTGAAG GGCTACGTGA CGATGGAGCC GTTCAAGGGC ATGCACCGCT TCGACTGCCG CCGCGAGATC GTGAAGAAGC TGGAGGAGAT GGGGCTGCTG CGCGGCGTGG AGCCGTACGA GTACCGCGTA GGCCGCTGCG AGCGCACTGG CGACATTGTG GAGCCGATGC TGATGCCGCA GTGGTTCATC GACTGCTCCG ATATGGCCCG CCGCTCCGTC GAGGCGGTGC GCAACGGCGA CCTGCGCCTG TACCCGCCTT CGCACGAGGC CGTCTGGTAC CACTGGCTGG AGAACATCAA ACCGTGGTGC GTGTCGCGTC AGCTCTGGTG GGGCCACCGC ATTCCTGCCT ACAAAGTTGT CGGCTCGCTG CCGAAAGACG TGGACCCATG GGTGGTCGCC CGCAATCTGC AGGAGGCGCA CGCGAAGGCT AAGAAGAAGT TCGGCCTGAC TGACGAGCAG GTCGCCGAGG CCTCGTTTGA GCAGGACCCG GATGTGCTGG ACACCTGGTT CAGCTCCGGA CTCTGGCCCT TCGCCACGCT TGGATGGCCC ACCGACTCGG ACGACATGAA GCGCTTCTTC CCGAACTCGC TGATGGAGAC CGGCCACGAC ATCCTGTTCT TCTGGGTGGC TCGCATGGTA ATGCTTTCGC TGCACTTCAC AAACAAGCTG CCGTACAAGG AGGTGTTTCT GCACGCAATG GTGCGCGACA AGAATGGCGA GAAGATGTCC AAGTCGAAGG GCAACGTCAT CGACCCACTC TACGTCATCC ACGGCGTTTC GCTGCAGACC CTCCACAACA CCGTGCGCAG CGGCAACCTG GGCGACAAGG AGGTGGAGAA GGCCATCAAG CAGCAGAAGG AGTTTTTCCC AGAAGGCATT CCAGAGTGCG GTAGCGATGC CCTGCGCTTC GGCCTCCTCT CCTACACCCA GTCTGGCCGC AGCGTGAACC TCGACATCCA ACGCGTCGTG GCCTACCGCC AGTTCTGCAA CAAGCTGTGG AACGTGGTGC GCTACGTGCT GTATCACGCC CTGGGCACTG ACTACGTGCC CAGCAAGCAG CAGTTCAGCC CCGCCGAGGA CGCCGCCATG CTGCCGCTCG AGTGCCGCTG GATTCTCTCG CGCCTCGACG CCGCCATCGC GGAGGCCACG CAGGGCATGT CGGAGGGCCT CTACGACTTC GCGTTGGCCA CCGGTGCCGT CTACCGCTTC TGGCTGCACG AGCTGTGCGA CGTGTTCCTT GAGTTGACGA AGCCGACCAT TCAGAAGGGC GGAGAGAAGC AGCAGCTGGT GCAAGACGTG CTGCTGCACG TCGTCGAGAA GGCGCTTCGG CTGCTACACC CGATGATGCC GTTTCTCACG GAGGAGCTTT GGCACCGCCT GCCCAACTAC AGCAGCTTCG GCAGCGAGTC GATCATGCTC GCCAAGTACC CGACACCGAG CGGGTGGTTG AGCGCGGCGT CGGATAGCGC CATGTCGATC ATTCTCGACG TCGTGCACAG CGTACGTTCC ACCAAGGCCT CGTACTCGCT AACGAACAAG CACAAGCCAG AGGTGTGGGT GACGGCGCAT ACGGCGGAGC TGCAGGAGCT GTTCGCGGCG GAAAAGATGA TGATCTCAAC CCTCGGTGTC GTGGGCGAGG TGACTGTCGT GTCCCCGGCC GAGGAGGCAG TGGCGGTGCC GAAGGGCTGC GGCTTCTCCG TGGTGACAAA GGAGGTGGGC GTGAACATGA TGCTCATGGG TTTCATCGAC GTCGGGAAGG AGGTGGCGAA GCTGGAGAAG CAGCTGGACG GCCTCATGAA GCAGATTGAG GGAATGAAGA AGAAGATGTC TATCCCTAAT TACGAAACTA AGGTGCCGGC CGAGATCCGC GTGACCAACA CGGAGAAGCT CAAGACGCTA GAGGCGCAGA GCGCGCAGCT GCGCGATGGC ATCGAGAAGA TGAGCAGCCT CAAGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT