LedoB.18809.a

3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) (EC 1.1.1.34)

CENTER ID: LedoB.18809.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.18809.a.B2.GE41119 mature protein; soluble domain 19 398
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LDBPK_303230
UniProt: E9BM66

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRSLLLACS AAKGGSWASM PDAEIMKQVE NKKIAFYGLE QALAPDYDRA IAIRREIVKK KMCPSTAATH PLERVPYKNY DWSSVVGQSC ENILGYVPVP VGLAGPLLLD GKEVALPMAT TEGALVASAH RGARAINLSG GCRTAVLKEG MTRAPVVEVN SFDEAITVIK FCEERFDVLK EAFESTTRFG KLLSIKCAMA GRQVHMRFSA FTGDAMGMNM ITKGCDKALQ VLQQHIPSVR VLTLSGNFCT DKKPSALNWV EGRGKSVVAE AVIKRDVVEG VLKCTVDSVV SLNVTKNLRG SALAGSIGGF NAHAANIVAA LYIATGQDPA QIVESATCMT TVDKAGEDLV ISLTMPSIEV GAVGGGTGLS SQRAMLELMG CAGSNKEDPG ANSRQIARVV AGAVICGELS LLSGLAAGHL LSAHMKLNRK PPTQ
NT Sequence
atgcgtcgct ctctgctgct tgcctgctcc gccgccaagg gtgggagctg ggcatccatg cccgatgcag agatcatgaa gcaggtggag aataagaaga ttgcatttta cggccttgag caggctcttg cacccgacta tgaccgcgcc atcgccattc gtcgtgagat tgtgaagaaa aagatgtgtc catcaaccgc tgccacacat ccgctggaga gggttcccta caagaactac gactggagca gtgttgtcgg ccagagctgc gagaacatcc tcggctacgt tccggtaccg gtagggttgg ctggcccgct gctgttggat ggaaaggaag tggcactgcc gatggcgacg acggagggtg cgctggtcgc gagtgcgcac cgcggtgctc gcgccatcaa cctgagcggc ggatgccgga cagcggtgct gaaggaaggc atgacgcgcg cccctgtagt ggaggtgaac tctttcgacg aggccatcac cgtgatcaag ttctgtgagg agcggttcga cgttctgaag gaggccttcg agtcgacgac ccgcttcggg aagttgctga gcatcaagtg cgccatggcg ggtcgccagg tgcatatgcg cttctctgcc tttactggag atgcgatggg catgaacatg atcacgaagg gctgtgacaa ggcgctgcag gtgctgcagc agcacattcc ctctgtgcga gtgctgacct tgtcgggcaa cttctgcact gacaagaagc cgtccgcact gaactgggtg gagggacgcg ggaagtcggt cgtggcggag gccgtcatca agcgtgatgt ggtcgagggt gttctcaaat gcacggtgga cagtgttgtg tcgctgaatg tgactaagaa cctgcgcggt tctgcgcttg ccggttccat cggcggcttc aacgcacacg ctgccaacat tgtggccgcg ctctacatcg ccactggcca ggaccccgca cagatagtgg agtccgcaac ctgcatgacg acggttgaca aggctggtga ggatctcgtg atcagcctca cgatgccgag catcgaggtc ggcgccgtag gcggtggcac aggtctgtcg tcgcagcggg ccatgctgga gctgatgggg tgcgcgggct ctaacaagga ggacccaggt gccaactccc gccagatcgc gcgcgtcgtc gctggcgcag tcatttgcgg ggagctgtcc ctcttgtccg gccttgcagc tggccacttg ctgagcgccc acatgaagct gaaccgcaag cctccgactc agtaa
Details for LedoB.18809.a.B2.GE41119
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS MPDAEIMKQV ENKKIAFYGL EQALAPDYDR AIAIRREIVK KKMCPSTAAT HPLERVPYKN YDWSSVVGQS CENILGYVPV PVGLAGPLLL DGKEVALPMA TTEGALVASA HRGARAINLS GGCRTAVLKE GMTRAPVVEV NSFDEAITVI KFCEERFDVL KEAFESTTRF GKLLSIKCAM AGRQVHMRFS AFTGDAMGMN MITKGCDKAL QVLQQHIPSV RVLTLSGNFC TDKKPSALNW VEGRGKSVVA EAVIKRDVVE GVLKCTVDSV VSLNVTKNLR GSALAGSIGG FNAHAANIVA ALYIATGQDP AQIVESATCM TTVDKAGEDL VISLTMPSIE VGAVGGGTGL SSQRAMLELM GCAGSNKEDP G
Validated NT Sequence
ggcacctggg tcctccttgt tagagcccgc gcaccccatc agctccagca tggcccgctg cgacgacaga cctgtgccac cgcctacggc gccgacctcg atgctcggca tcgtgaggct gatcacgaga tcctcaccag ccttgtcaac cgtcgtcatg caggttgcgg actccactat ctgtgcgggg tcctggccag tggcgatgta gagcgcggcc acgatgttgg cagcgtgtgc gttgaagccg ccgatggaac cggcaagcgc agaaccgcgc aggttcttag tcacattcag cgacacaaca ctgtccaccg tgcatttgag aacaccctcg accacatcac gcttgatgac ggcctccgcc acgaccgact tcccgcgtcc ctccacccag ttcagtgcgg acggcttctt gtcagtgcag aagttgcccg acaaggtcag cactcgcaca gagggaatgt gctgctgcag cacctgcagc gccttgtcac agcccttcgt gatcatgttc atgcccatgg catctccagt aaaggcagag aagcgcatat gcacctggcg acccgccatg gcgcacttga tgctcagcaa cttcccgaag cgggtcgtcg actcgaaggc ctccttcaga acgtcgaacc gctcctcaca gaacttgatc acggtgatgg cctcgtcgaa agagttcacc tccactacag gggcgcgcgt catgccttcc ttcagcaccg ctgtccggca tccgccgctc aggttgatgg cgcgagcacc gcggtgcgca ctcgcgacca gcgcgccctc cgtcgtcgcc atcggcagtg ccacttcctt tccatccaac agcagcgggc cagccaaccc taccggtacc ggaacgtagc cgaggatgtt ctcgcagctc tggccgacaa cactgctcca gtcgtagttc ttgtagggaa ccctctccag cggatgtgtg gcagcggttg atggacacat ctttttcttc acaatctcac gacgaatggc gatggcgcgg tcatagtcgg gtgcaagagc ctgctcaagg ccgtaaaatg caatcttctt attctccacc tgcttcatga tctctgcatc gggcatggac atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHSM PDAEIMKQVE NKKIAFYGLE QALAPDYDRA IAIRREIVKK KMCPSTAATH PLERVPYKNY DWSSVVGQSC ENILGYVPVP VGLAGPLLLD GKEVALPMAT TEGALVASAH RGARAINLSG GCRTAVLKEG MTRAPVVEVN SFDEAITVIK FCEERFDVLK EAFESTTRFG KLLSIKCAMA GRQVHMRFSA FTGDAMGMNM ITKGCDKALQ VLQQHIPSVR VLTLSGNFCT DKKPSALNWV EGRGKSVVAE AVIKRDVVEG VLKCTVDSVV SLNVTKNLRG SALAGSIGGF NAHAANIVAA LYIATGQDPA QIVESATCMT TVDKAGEDLV ISLTMPSIEV GAVGGGTGLS SQRAMLELMG CAGSNKEDPG ANSRQIAR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccatgc ccgatgcaga gatcatgaag caggtggaga ataagaagat tgcattttac ggccttgagc aggctcttgc acccgactat gaccgcgcca tcgccattcg tcgtgagatt gtgaagaaaa agatgtgtcc atcaaccgct gccacacatc cgctggagag ggttccctac aagaactacg actggagcag tgttgtcggc cagagctgcg agaacatcct cggctacgtt ccggtaccgg tagggttggc tggcccgctg ctgttggatg gaaaggaagt ggcactgccg atggcgacga cggagggtgc gctggtcgcg agtgcgcacc gcggtgctcg cgccatcaac ctgagcggcg gatgccggac agcggtgctg aaggaaggca tgacgcgcgc ccctgtagtg gaggtgaact ctttcgacga ggccatcacc gtgatcaagt tctgtgagga gcggttcgac gttctgaagg aggccttcga gtcgacgacc cgcttcggga agttgctgag catcaagtgc gccatggcgg gtcgccaggt gcatatgcgc ttctctgcct ttactggaga tgcgatgggc atgaacatga tcacgaaggg ctgtgacaag gcgctgcagg tgctgcagca gcacattccc tctgtgcgag tgctgacctt gtcgggcaac ttctgcactg acaagaagcc gtccgcactg aactgggtgg agggacgcgg gaagtcggtc gtggcggagg ccgtcatcaa gcgtgatgtg gtcgagggtg ttctcaaatg cacggtggac agtgttgtgt cgctgaatgt gactaagaac ctgcgcggtt ctgcgcttgc cggttccatc ggcggcttca acgcacacgc tgccaacatt gtggccgcgc tctacatcgc cactggccag gaccccgcac agatagtgga gtccgcaacc tgcatgacga cggttgacaa ggctggtgag gatctcgtga tcagcctcac gatgccgagc atcgaggtcg gcgccgtagg cggtggcaca ggtctgtcgt cgcagcgggc catgctggag ctgatggggt gcgcgggctc taacaaggag gacccaggtg ccaactcccg ccagatcgcg cgctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat