SSGCID: Target Page

LedoB.18771.a

leucyl-tRNA synthetase, putative

CENTER ID:	LedoB.18771.a
ORGANISM:	Leishmania donovani BPK282A1
ASSOCIATED DISEASE:	Leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LedoB.18771.a.B1.GE42681	full length		1	1075
LedoB.18771.a.B2.GE43053	XtalPred domain		96	1045

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LdBPK_131000.1
UniProt:	E9BBH4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTARRDALV AIEQEKQAYW AAEKLHEFDA PAPGEARPAK YLTTFPFPYM NGKLHLGHGF SLTKAEFASR FQRMMGRRSL WPFGFHVTGT PIAACAQKIA KEMQQYGNPP QFPAELLEDK PKAMVVKEPT EALGQHKSKR GKSGPAKPQW LIMQSMGIPD SEIAKFADPQ YWLDYFPPIA MEDLKSFGCH IDWRRSFMTT ERSPYFDRFV RWQFNILRRE NLLNYGKRYC VYSPRDGQPC ADHDRASGEG VLPQEYALVK LIVQNPLAQP CFAEHKDIIG DRNVVLPGAT LRPETVIGQT NCWVSNKLNY KGYYVRNAKG EDEVYIMTAR AARNIAYQNF YVNGQVGTDP EPLFEVEGAS MVGIPLSAPL APYTTIYTLP MATITEAKGT GVVMSVPSDS PDDYINFTQL LNKPEYRVKL GIKDEWVVPF AMIPIIDIPE LGTEGAKFMC EKLKINGPNA TELLEEAKKV CYQQGFYHGT MISGPYAGVK VSEAKVKTHR DMEAADQCIR YYEPVRQVIS RSGDECVVAL CDQWYLEYGK DDWKKLVQDH IKKMDMFFPG VRNGFEETLN WLADWPCSRT FGLGTYLPCD ASHTMIIDSL SDSTIYMAYY TIDRFFNVGA DGSTDLCGKA DNPYGLAPEM FTDEVFEYIY HGVGDAATVA GAVRMPVESL KLMRNEFEYW YPVDLRCSGK DLIQNHLTMF LYNHAAIWPT DESKWPRAIF CNGHIQVDNE KMAKSRGNFI SLREAMDMYG ADATRLTCAD AGDSMDDANF VRETAAGFVL KMTTLLEQAK ETVERTDLRS GGYNEFDKIF NNTMNTIITR TEGFYHRMQF RMVLNTAFHE LSNEYSQYKM YCDDLNVHAQ LGRRYYEVVA LMMMPLAPHT MEHLWQNILQ HEGSVVTQPF PKPTAKLDYS LIVASRVMTI ALKEIRSQVI KNAKKRGPVE EVIVYTRREY TDWQRQALEI LHELYEANGN TFPEDMAKQV VARDTKIMSK ALMAFMSFVK GNVEKYGEQA MSTQPVIDDA VMLSNVTHNL SRLAGVPVVR ILSAEDETYK EHDAARRKCL PGEPSVALPP VVKRE

NT Sequence

atgtccacgg cacgtcgcga tgcgctcgtg gcgattgagc aggagaagca agcctactgg gccgcggaga agttgcatga gttcgacgcc ccagcgcctg gggaggcgcg accggcgaag tatctgacca cgttcccctt cccgtacatg aacggcaagc ttcacctcgg tcatggcttc tcgctcacga aggccgagtt cgcctcgcgc ttccagcgca tgatgggccg ccgttcgctc tggcccttcg gcttccacgt caccggcacc ccgatcgctg cgtgcgcgca gaaaatcgcc aaggagatgc agcagtacgg caacccgccg cagttcccgg cggagctgct cgaggacaag cccaaggcga tggtggtcaa ggagccgacg gaggcgctag ggcagcacaa gagcaagcga ggcaagagcg ggccggcgaa accgcagtgg ctcatcatgc agagcatggg catccccgac tcggagatcg cgaagttcgc cgacccgcag tactggctgg actacttccc gcccatcgcg atggaggact tgaagagttt tggctgccac atcgactggc gccgctcctt catgacgacg gagcgtagcc catactttga ccgcttcgtg cggtggcagt tcaacatcct gcgccgcgag aacctgctca actacggcaa gcgttactgt gtgtactcgc cgcgcgacgg ccagccgtgc gccgatcacg atcgcgccag cggtgagggt gtgctgccgc aggagtacgc attggtgaag ctcatcgtcc agaacccact cgcgcagccg tgcttcgccg agcacaagga cattatcggc gaccgcaacg tcgtgctgcc cggcgccaca ctccgtcctg agacggtcat cggtcagacg aactgctggg tcagcaacaa gctcaactac aaggggtact acgtgcgcaa cgcgaaggga gaagacgaag tctacatcat gacggcccgc gccgcgcgca atatagcgta ccagaacttc tacgtaaacg gccaggtcgg caccgatccg gagccgctct tcgaggtgga gggagcaagc atggtcggca taccgctttc ggccccgctg gcgccttaca cgacaatcta cacgctgcct atggccacca tcacggaggc gaagggtacc ggcgtcgtca tgtctgtgcc gtccgactcg ccggacgact acatcaactt tacccagctg ctgaacaagc cggaataccg cgtgaagctc ggcatcaaag acgagtgggt agtgccgttc gcgatgattc cgatcataga cattcccgag ctgggcaccg agggcgccaa gttcatgtgc gagaagctca agatcaacgg cccgaacgcg acggagctgc tagaggaggc gaagaaagtg tgctaccagc agggcttcta ccacggcacc atgatcagtg gcccctacgc cggcgtcaag gtgagcgagg ccaaggtgaa gacgcatcgt gacatggagg cggcggacca gtgcatccgc tactacgagc cggtgcgcca ggtgatcagc cgcagcggtg acgagtgcgt cgtcgcgctg tgcgaccagt ggtacctcga gtatggcaag gacgattgga agaagctggt gcaggaccac atcaagaaga tggacatgtt cttccccggt gtgcgcaacg gcttcgagga gacgctgaac tggctggcag actggccgtg cagccgcacc ttcggtctcg gcacctacct gccgtgcgac gcgtctcaca cgatgatcat tgacagcctg tcggactcca ccatctacat ggcctactac acgatcgacc gcttcttcaa cgtcggcgcc gacggttcga ccgacctgtg cggcaaggcg gacaacccgt acggtctggc gccagagatg ttcacggacg aggtgttcga gtacatctac cacggcgtcg gcgacgcggc caccgtcgcg ggtgcggtga ggatgccggt cgagtcgctg aagctgatgc gcaacgagtt cgagtactgg taccctgtcg acctgcgctg ctcgggcaag gacctcatcc agaaccacct gacgatgttc ctctacaacc acgccgccat ctggccgacg gacgagagca agtggccgcg ggcaatcttc tgcaacggcc acatccaggt cgataacgaa aagatggcca aatcgagggg gaactttatc tccttgcgtg aggcgatgga catgtacggc gctgacgcca cgcgtcttac ctgcgccgat gccggtgaca gcatggacga cgccaacttc gtgcgcgaga cggcggctgg gtttgtgctg aagatgacga cgctgctgga gcaggcgaag gagacggttg agcgcaccga cctgcgcagc ggcggctaca acgagttcga caagatcttc aataacacaa tgaacacgat catcacccgc accgagggct tctaccaccg catgcagttc cgcatggtgc tcaacacagc cttccacgag ctgtccaacg agtacagtca gtataagatg tactgcgacg acctcaacgt acacgcgcag ctcggccgcc gctactacga ggtggtggcg ctgatgatga tgccactggc gccacacacg atggagcatc tgtggcagaa catcctccag cacgagggct ctgtcgtcac gcagccgttc ccgaagccga cggcgaagct ggactactcg ctgatcgtcg caagccgggt catgacgatc gctctcaagg agattcggtc gcaggtgatc aagaacgcga agaagcgcgg tccggtcgag gaggtcatcg tatacacgcg tcgcgagtac acggactggc agcggcaggc actcgagata ctgcacgagc tctacgaggc gaacggcaac acgttcccgg aggatatggc gaagcaagtt gtcgcccgcg acaccaagat catgagcaag gccctgatgg ccttcatgtc cttcgtcaaa ggcaacgtag agaagtacgg ggagcaggcc atgtcgacgc agcccgtcat cgacgatgcc gtgatgttgt cgaacgtcac gcacaacctc agccgcctcg ccggcgtacc ggtggtgcgc atcctatccg ccgaagacga gacttacaag gagcacgacg cggcacgccg caagtgcctg cctggtgagc cgtccgttgc cttgccgcca gtagtgaagc gcgagtag

Details for LedoB.18771.a.B1.GE42681

HARVESTED ON:	9/28/2018
SEQUENCED ON:	10/17/2018
EXPECTED MW:	123kDa
OBSERVED MW:	122kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS TARRDALVAI EQEKQAYWAA EKLHEFDAPA PGEARPAKYL TTFPFPYMNG KLHLGHGFSL TKAEFASRFQ RMMGRRSLWP FGFHVTGTPI AACAQKIAKE MQQYGNPPQF PAELLEDKPK AMVVKEPTEA LGQHKSKRGK SGPAKPQWLI MQSMGIPDSE IAKFADPQYW LDYFPPIAME DLKSFGCHID WRRSFMTTER SPYFDRFVRW QFNILRRENL LNYGKRYCVY SPRDGQPCAD HDRASGEGVL PQEYALVKLI VQNPLAQPCF AEHKDIIGDR NVVLPGATLR PETVIGQTNC WVSNKLNYKG YYVRNAKGED EVYIMTARAA RNIAYQNFYV NGQVGTDPEP LFEVEGASMV GIPLSAPLAP YTTIYTLPMA TITEAKGTGV VMSVPSDSPD DYINFTQLLN KPEYRVKLGI KDEWVVPFAM IPIIDIPELG TEGAKFMCEK LKINGPNATE LLEEAKKVCY QQGFYHGTMI SGPYAGVKVS EAKVKTHRDM EAADQCIRYY EPVRQVISRS GDECVVALCD QWYLEYGKDD WKKLVQDHIK KMDMFFPGVR NGFEETLNWL ADWPCSRTFG LGTYLPCDAS HTMIIDSLSD STIYMAYYTI DRFFNVGADG STDLCGKADN PYGLAPEMFT DEVFEYIYHG VGDAATVAGA VRMPVESLKL MRNEFEYWYP VDLRCSGKDL IQNHLTMFLY NHAAIWPTDE SKWPRAIFCN GHIQVDNEKM AKSRGNFISL REAMDMYGAD ATRLTCADAG DSMDDANFVR ETAAGFVLKM TTLLEQAKET VERTDLRSGG YNEFDKIFNN TMNTIITRTE GFYHRMQFRM VLNTAFHELS NEYSQYKMYC DDLNVHAQLG RRYYEVVALM MMPLAPHTME HLWQNILQHE GSVVTQPFPK PTAKLDYSLI VASRVMTIAL KEIRSQVIKN AKKRGPVEEV IVYTRREYTD WQRQALEILH ELYEANGNTF PEDMAKQVVA RDTKIMSKAL MAFMSFVKGN VEKYGEQAMS TQPVIDDAVM LSNVTHNLSR LAGVPVVRIL SAEDETYKEH DAARRKCLPG EPSVALPPVV KRE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca cggcacgtcg cgatgcgctc gtggcgattg agcaggagaa gcaagcctac tgggccgcgg agaagttgca tgagttcgac gccccagcgc ctggggaggc gcgaccggcg aagtatctga ccacgttccc cttcccgtac atgaacggca agcttcacct cggtcatggc ttctcgctca cgaaggccga gttcgcctcg cgcttccagc gcatgatggg ccgccgttcg ctctggccct tcggcttcca cgtcaccggc accccgatcg ctgcgtgcgc gcagaaaatc gccaaggaga tgcagcagta cggcaacccg ccgcagttcc cggcggagct gctcgaggac aagcccaagg cgatggtggt caaggagccg acggaggcgc tagggcagca caagagcaag cgaggcaaga gcgggccggc gaaaccgcag tggctcatca tgcagagcat gggcatcccc gactcggaga tcgcgaagtt cgccgacccg cagtactggc tggactactt cccgcccatc gcgatggagg acttgaagag ttttggctgc cacatcgact ggcgccgctc cttcatgacg acggagcgta gcccatactt tgaccgcttc gtgcggtggc agttcaacat cctgcgccgc gagaacctgc tcaactacgg caagcgttac tgtgtgtact cgccgcgcga cggccagccg tgcgccgatc acgatcgcgc cagcggtgag ggtgtgctgc cgcaggagta cgcattggtg aagctcatcg tccagaaccc actcgcgcag ccgtgcttcg ccgagcacaa ggacattatc ggcgaccgca acgtcgtgct gcccggcgcc acactccgtc ctgagacggt catcggtcag acgaactgct gggtcagcaa caagctcaac tacaaggggt actacgtgcg caacgcgaag ggagaagacg aagtctacat catgacggcc cgcgccgcgc gcaatatagc gtaccagaac ttctacgtaa acggccaggt cggcaccgat ccggagccgc tcttcgaggt ggagggagca agcatggtcg gcataccgct ttcggccccg ctggcgcctt acacgacaat ctacacgctg cctatggcca ccatcacgga ggcgaagggt accggcgtcg tcatgtctgt gccgtccgac tcgccggacg actacatcaa ctttacccag ctgctgaaca agccggaata ccgcgtgaag ctcggcatca aagacgagtg ggtagtgccg ttcgcgatga ttccgatcat agacattccc gagctgggca ccgagggcgc caagttcatg tgcgagaagc tcaagatcaa cggcccgaac gcgacggagc tgctagagga ggcgaagaaa gtgtgctacc agcagggctt ctaccacggc accatgatca gtggccccta cgccggcgtc aaggtgagcg aggccaaggt gaagacgcat cgtgacatgg aggcggcgga ccagtgcatc cgctactacg agccggtgcg ccaggtgatc agccgcagcg gtgacgagtg cgtcgtcgcg ctgtgcgacc agtggtacct cgagtatggc aaggacgatt ggaagaagct ggtgcaggac cacatcaaga agatggacat gttcttcccc ggtgtgcgca acggcttcga ggagacgctg aactggctgg cagactggcc gtgcagccgc accttcggtc tcggcaccta cctgccgtgc gacgcgtctc acacgatgat cattgacagc ctgtcggact ccaccatcta catggcctac tacacgatcg accgcttctt caacgtcggc gccgacggtt cgaccgacct gtgcggcaag gcggacaacc cgtacggtct ggcgccagag atgttcacgg acgaggtgtt cgagtacatc taccacggcg tcggcgacgc ggccaccgtc gcgggtgcgg tgaggatgcc ggtcgagtcg ctgaagctga tgcgcaacga gttcgagtac tggtaccctg tcgacctgcg ctgctcgggc aaggacctca tccagaacca cctgacgatg ttcctctaca accacgccgc catctggccg acggacgaga gcaagtggcc gcgggcaatc ttctgcaacg gccacatcca ggtcgataac gaaaagatgg ccaaatcgag ggggaacttt atctccttgc gtgaggcgat ggacatgtac ggcgctgacg ccacgcgtct tacctgcgcc gatgccggtg acagcatgga cgacgccaac ttcgtgcgcg agacggcggc tgggtttgtg ctgaagatga cgacgctgct ggagcaggcg aaggagacgg ttgagcgcac cgacctgcgc agcggcggct acaacgagtt cgacaagatc ttcaataaca caatgaacac gatcatcacc cgcaccgagg gcttctacca ccgcatgcag ttccgcatgg tgctcaacac agccttccac gagctgtcca acgagtacag tcagtataag atgtactgcg acgacctcaa cgtacacgcg cagctcggcc gccgctacta cgaggtggtg gcgctgatga tgatgccact ggcgccacac acgatggagc atctgtggca gaacatcctc cagcacgagg gctctgtcgt cacgcagccg ttcccgaagc cgacggcgaa gctggactac tcgctgatcg tcgcaagccg ggtcatgacg atcgctctca aggagattcg gtcgcaggtg atcaagaacg cgaagaagcg cggtccggtc gaggaggtca tcgtatacac gcgtcgcgag tacacggact ggcagcggca ggcactcgag atactgcacg agctctacga ggcgaacggc aacacgttcc cggaggatat ggcgaagcaa gttgtcgccc gcgacaccaa gatcatgagc aaggccctga tggccttcat gtccttcgtc aaaggcaacg tagagaagta cggggagcag gccatgtcga cgcagcccgt catcgacgat gccgtgatgt tgtcgaacgt cacgcacaac ctcagccgcc tcgccggcgt accggtggtg cgcatcctat ccgccgaaga cgagacttac aaggagcacg acgcggcacg ccgcaagtgc ctgcctggtg agccgtccgt tgccttgccg ccagtagtga agcgcgagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for LedoB.18771.a.B2.GE43053

HARVESTED ON:	4/19/2019
SEQUENCED ON:	4/23/2019
EXPECTED MW:	109kDa
OBSERVED MW:	109kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHAQ KIAKEMQQYG NPPQFPAELL EDKPKAMVVK EPTEALGQHK SKRGKSGPAK PQWLIMQSMG IPDSEIAKFA DPQYWLDYFP PIAMEDLKSF GCHIDWRRSF MTTERSPYFD RFVRWQFNIL RRENLLNYGK RYCVYSPRDG QPCADHDRAS GEGVLPQEYA LVKLIVQNPL AQPCFAEHKD IIGDRNVVLP GATLRPETVI GQTNCWVSNK LNYKGYYVRN AKGEDEVYIM TARAARNIAY QNFYVNGQVG TDPEPLFEVE GASMVGIPLS APLAPYTTIY TLPMATITEA KGTGVVMSVP SDSPDDYINF TQLLNKPEYR VKLGIKDEWV VPFAMIPIID IPELGTEGAK FMCEKLKING PNATELLEEA KKVCYQQGFY HGTMISGPYA GVKVSEAKVK THRDMEAADQ CIRYYEPVRQ VISRSGDECV VALCDQWYLE YGKDDWKKLV QDHIKKMDMF FPGVRNGFEE TLNWLADWPC SRTFGLGTYL PCDASHTMII DSLSDSTIYM AYYTIDRFFN VGADGSTDLC GKADNPYGLA PEMFTDEVFE YIYHGVGDAA TVAGAVRMPV ESLKLMRNEF EYWYPVDLRC SGKDLIQNHL TMFLYNHAAI WPTDESKWPR AIFCNGHIQV DNEKMAKSRG NFISLREAMD MYGADATRLT CADAGDSMDD ANFVRETAAG FVLKMTTLLE QAKETVERTD LRSGGYNEFD KIFNNTMNTI ITRTEGFYHR MQFRMVLNTA FHELSNEYSQ YKMYCDDLNV HAQLGRRYYE VVALMMMPLA PHTMEHLWQN ILQHEGSVVT QPFPKPTAKL DYSLIVASRV MTIALKEIRS QVIKNAKKRG PVEEVIVYTR REYTDWQRQA LEILHELYEA NGNTFPEDMA KQVVARDTKI MSKALMAFMS FVKGNVEKYG EQAMSTQPVI DDAVMLSNVT HNLSRLAGVP VVRILSAE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgcaga aaatcgccaa ggagatgcag cagtacggca acccgccgca gttcccggcg gagctgctcg aggacaagcc caaggcgatg gtggtcaagg agccgacgga ggcgctaggg cagcacaaga gcaagcgagg caagagcggg ccggcgaaac cgcagtggct catcatgcag agcatgggca tccccgactc ggagatcgcg aagttcgccg acccgcagta ctggctggac tacttcccgc ccatcgcgat ggaggacttg aagagttttg gctgccacat cgactggcgc cgctccttca tgacgacgga gcgtagccca tactttgacc gcttcgtgcg gtggcagttc aacatcctgc gccgcgagaa cctgctcaac tacggcaagc gttactgtgt gtactcgccg cgcgacggcc agccgtgcgc cgatcacgat cgcgccagcg gtgagggtgt gctgccgcag gagtacgcat tggtgaagct catcgtccag aacccactcg cgcagccgtg cttcgccgag cacaaggaca ttatcggcga ccgcaacgtc gtgctgcccg gcgccacact ccgtcctgag acggtcatcg gtcagacgaa ctgctgggtc agcaacaagc tcaactacaa ggggtactac gtgcgcaacg cgaagggaga agacgaagtc tacatcatga cggcccgcgc cgcgcgcaat atagcgtacc agaacttcta cgtaaacggc caggtcggca ccgatccgga gccgctcttc gaggtggagg gagcaagcat ggtcggcata ccgctttcgg ccccgctggc gccttacacg acaatctaca cgctgcctat ggccaccatc acggaggcga agggtaccgg cgtcgtcatg tctgtgccgt ccgactcgcc ggacgactac atcaacttta cccagctgct gaacaagccg gaataccgcg tgaagctcgg catcaaagac gagtgggtag tgccgttcgc gatgattccg atcatagaca ttcccgagct gggcaccgag ggcgccaagt tcatgtgcga gaagctcaag atcaacggcc cgaacgcgac ggagctgcta gaggaggcga agaaagtgtg ctaccagcag ggcttctacc acggcaccat gatcagtggc ccctacgccg gcgtcaaggt gagcgaggcc aaggtgaaga cgcatcgtga catggaggcg gcggaccagt gcatccgcta ctacgagccg gtgcgccagg tgatcagccg cagcggtgac gagtgcgtcg tcgcgctgtg cgaccagtgg tacctcgagt atggcaagga cgattggaag aagctggtgc aggaccacat caagaagatg gacatgttct tccccggtgt gcgcaacggc ttcgaggaga cgctgaactg gctggcagac tggccgtgca gccgcacctt cggtctcggc acctacctgc cgtgcgacgc gtctcacacg atgatcattg acagcctgtc ggactccacc atctacatgg cctactacac gatcgaccgc ttcttcaacg tcggcgccga cggttcgacc gacctgtgcg gcaaggcgga caacccgtac ggtctggcgc cagagatgtt cacggacgag gtgttcgagt acatctacca cggcgtcggc gacgcggcca ccgtcgcggg tgcggtgagg atgccggtcg agtcgctgaa gctgatgcgc aacgagttcg agtactggta ccctgtcgac ctgcgctgct cgggcaagga cctcatccag aaccacctga cgatgttcct ctacaaccac gccgccatct ggccgacgga cgagagcaag tggccgcggg caatcttctg caacggccac atccaggtcg ataacgaaaa gatggccaaa tcgaggggga actttatctc cttgcgtgag gcgatggaca tgtacggcgc tgacgccacg cgtcttacct gcgccgatgc cggtgacagc atggacgacg ccaacttcgt gcgcgagacg gcggctgggt ttgtgctgaa gatgacgacg ctgctggagc aggcgaagga gacggttgag cgcaccgacc tgcgcagcgg cggctacaac gagttcgaca agatcttcaa taacacaatg aacacgatca tcacccgcac cgagggcttc taccaccgca tgcagttccg catggtgctc aacacagcct tccacgagct gtccaacgag tacagtcagt ataagatgta ctgcgacgac ctcaacgtac acgcgcagct cggccgccgc tactacgagg tggtggcgct gatgatgatg ccactggcgc cacacacgat ggagcatctg tggcagaaca tcctccagca cgagggctct gtcgtcacgc agccgttccc gaagccgacg gcgaagctgg actactcgct gatcgtcgca agccgggtca tgacgatcgc tctcaaggag attcggtcgc aggtgatcaa gaacgcgaag aagcgcggtc cggtcgagga ggtcatcgta tacacgcgtc gcgagtacac ggactggcag cggcaggcac tcgagatact gcacgagctc tacgaggcga acggcaacac gttcccggag gatatggcga agcaagttgt cgcccgcgac accaagatca tgagcaaggc cctgatggcc ttcatgtcct tcgtcaaagg caacgtagag aagtacgggg agcaggccat gtcgacgcag cccgtcatcg acgatgccgt gatgttgtcg aacgtcacgc acaacctcag ccgcctcgcc ggcgtaccgg tggtgcgcat cctatccgcc gaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat