SSGCID: Target Page

LedoB.18754.a

Phenylalanyl-tRNA synthetase, putative (PheRS)

CENTER ID:	LedoB.18754.a
ORGANISM:	Leishmania donovani BPK282A1
ASSOCIATED DISEASE:	Leishmaniasis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LedoB.18754.a.B1.GE41816	full length		1	633

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LedoB.18754.a.B1.PW38634	full length		1	633

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LdBPK_191010.1
UniProt:	E9BE94

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPTLAVAKDY ICRLIGKSYT DEEFNDLCFQ FGLELDDITS EKEMFMREQG KSAKASVAPA EAPAHLSEEK LYKIDTPANR HDLLTAEGMA CALKVFMGTM PLPNYRVLNR ANPLYRMTVE KSVKNVRDYV VCAVLHNIRF NERSYNSFID YQEKLHAGLA RRRTLASVGT HDLDKVNTTD FIFACRPRET IRFVPLRQTR ELNCSGNGLA QYYAEDRHIG KYVPLISSFP TYPVVYDGTG QNVLSLPPII NSRYSAISVD TRNIFIECTA PDHYKAQVLV NQLVCAFSAY CEDPFTVEAV QVNYEEPTPD GTRSLVTPTM ETKEMTMSIA RLNSIIGIEM KSGAECAQLL KKMMYTIKET TESEVTVTVP PMRTDVIGVA DVMEDVAIAY GYDNIAYVEC KTHGPVTQTP LSKVAQLLRT EMACAGYTEL LTLSLCSRAD AFESLGRVDN DVAAHIANPQ TMEFQVCRPS LLPGILKTLA TNKSKPLPQR FFECADVVLL DNERNFPPVL ELKADYASCG ARNQRHLAAA HCNGSSSGFE SIHGLTELAL LKLGVPSKAE IAEDYEGDYY TLEKGEDGAF FPGRAMDIIL HRAGQAVRIG HLGVIHPNTL KAYDIPSPCS YMELNIQFLC VPL

NT Sequence

atgccgacgt tggccgtggc gaaggactac atctgccggc tgatcggcaa gtcctacaca gacgaggagt tcaacgacct gtgtttccag tttggcctgg agctcgatga cattacgagt gagaaagaaa tgttcatgag ggagcagggc aagagcgcaa aggcgagcgt ggctccggcg gaggcccctg cgcacctttc ggaggagaag ctctacaaaa ttgacacccc ggcaaaccgt cacgaccttc tcaccgcgga gggcatggcc tgcgctctaa aggtgttcat ggggacaatg ccgctcccca actaccgcgt cctcaaccgt gccaacccgc tctaccgcat gaccgtggag aaaagcgtga agaacgtgcg cgactacgtt gtgtgcgctg tcctgcacaa catccgcttc aatgagcgca gttacaacag cttcatcgac taccaggaga agctccacgc cgggttggcc cgccgccgca cgctggcctc ggttggcacc cacgacctag acaaggtgaa cacgacggac tttatcttcg cctgccgccc gcgcgagacc atccggttcg ttccgctgcg gcagacaagg gagctgaact gttcaggcaa cggcctcgcg cagtactacg ccgaggaccg ccacatcggc aagtatgtac cgctgatctc ctcctttccg acctacccgg ttgtgtacga cggtaccggc cagaacgtgc tctcgttgcc gccgatcatc aactcgcgct actcggccat ctccgtggac accaggaaca tcttcattga gtgcacggcc ccggaccact acaaggcgca ggtgctggtg aaccagctcg tgtgtgcctt ctcggcctac tgcgaggatc ccttcacagt ggaggcggtg caggtgaact atgaggagcc gacgccagac ggaacaaggt cgcttgtgac accaacgatg gagacgaagg aaatgacgat gagcatcgca cgcctcaact cgatcatcgg catcgaaatg aaaagtggtg ctgagtgcgc gcagctgctg aagaagatga tgtacacgat caaggagacg acggagagtg aggtgacggt gacggtgccg ccaatgcgca cggacgtcat cggcgtcgcg gacgtgatgg aggatgtcgc catcgcgtac ggctacgaca acatcgccta cgtggagtgc aagacccatg gccctgtgac gcagacgccg ctgagcaagg tggcgcagct gctgcgcact gagatggcct gcgcaggcta cacggagctg ctgacgctgt cactctgctc gcgcgccgat gcgttcgaga gcctgggccg cgtggacaac gacgtcgcgg cgcacatcgc caacccgcag acgatggagt tccaggtgtg ccgcccatca ctgctccccg gtattctgaa gacgctcgcg acgaacaagt cgaagcctct accgcagcgc ttcttcgagt gcgcggacgt cgtcctgctg gacaacgaac ggaatttccc accggtgctg gagttgaagg ccgactacgc gtcctgtggt gcgcggaacc agagacacct cgccgccgcg cactgcaacg gcagctctag cggatttgag agcattcacg gcctcaccga gctcgcccta ctcaagcttg gcgtcccgtc gaaagcggag atagcagagg actacgaagg cgactactac acactggaga agggcgagga cggcgccttc tttcctggcc gcgctatgga catcattctt caccgcgctg gtcaggcagt gcgcatcggg cacctcggcg tcatccaccc gaacacgctc aaggcgtacg acattccgag cccgtgctct tacatggagc tcaacatcca gttcctgtgc gtccctctgt aa

Details for LedoB.18754.a.B1.GE41816

HARVESTED ON:	8/7/2017
SEQUENCED ON:	9/20/2017
EXPECTED MW:	71kDa
OBSERVED MW:	71kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	86
PERCENT COVERAGE:	49

Validated AA Sequence

Validated NT Sequence

atggctcacc accaccacca ccatatgccg acgttggccg tggcgaagga ctacatctgc cggctgatcg gcaagtccta cacagacgag gagttcaacg acctgtgttt ccagtttggc ctggagctcg atgacattac gagtgagaaa gaaatgttca tgagggagca gggcaagagc gcaaaggcga gcgtggctcc ggcggaggcc cctgcgcacc tttcggagga gaagctctac aaaattgaca ccccggcaaa ccgtcacgac cttctcaccg cggagggcat ggcctgcgct ctaaaggtgt tcatggggac aatgccgctc cccaactacc gcgtcctcaa ccgtgccaac ccgctctacc gcatgaccgt ggagaaaagc gtgaagaacg tgcgcgacta cgttgtgtgc gctgtactgc acaacatccg cttcaacgag cgcagttaca acagcttcat cgactaccag gagaagctcc acgccgggtt ggcccgccgc cgcacgctgg cctcggttgg cacccacgac ctagacaagg tgaacacgac ggactttatc ttcgcctgcc gcccgcgcga gaccatccgg ttcgttccgc tgcggcagac aagggagctg aactgctcag gcaacggcct cacgcagtac tacgccgagg accgccacat cggcaagtat gtaccgctaa tctcctcctt tccgacctac ccggttgtgt acgacggtac cggccagaac gtgctctcgt tgccgccgat catcaactcg cgctactcnn nnatctccgt nnncancagg aanatcntnn ntgagtgcac ggccccgnnn nnntacnagn cgnnnnnnnn tggtnnnnnn nnnnnnnnnn nnnnnnnnnn cntannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnc

Expected Protein Sequence

MAHHHHHHMP TLAVAKDYIC RLIGKSYTDE EFNDLCFQFG LELDDITSEK EMFMREQGKS AKASVAPAEA PAHLSEEKLY KIDTPANRHD LLTAEGMACA LKVFMGTMPL PNYRVLNRAN PLYRMTVEKS VKNVRDYVVC AVLHNIRFNE RSYNSFIDYQ EKLHAGLARR RTLASVGTHD LDKVNTTDFI FACRPRETIR FVPLRQTREL NCSGNGLAQY YAEDRHIGKY VPLISSFPTY PVVYDGTGQN VLSLPPIINS RYSAISVDTR NIFIECTAPD HYKAQVLVNQ LVCAFSAYCE DPFTVEAVQV NYEEPTPDGT RSLVTPTMET KEMTMSIARL NSIIGIEMKS GAECAQLLKK MMYTIKETTE SEVTVTVPPM RTDVIGVADV MEDVAIAYGY DNIAYVECKT HGPVTQTPLS KVAQLLRTEM ACAGYTELLT LSLCSRADAF ESLGRVDNDV AAHIANPQTM EFQVCRPSLL PGILKTLATN KSKPLPQRFF ECADVVLLDN ERNFPPVLEL KADYASCGAR NQRHLAAAHC NGSSSGFESI HGLTELALLK LGVPSKAEIA EDYEGDYYTL EKGEDGAFFP GRAMDIILHR AGQAVRIGHL GVIHPNTLKA YDIPSPCSYM ELNIQFLCVP L

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccga cgttggccgt ggcgaaggac tacatctgcc ggctgatcgg caagtcctac acagacgagg agttcaacga cctgtgtttc cagtttggcc tggagctcga tgacattacg agtgagaaag aaatgttcat gagggagcag ggcaagagcg caaaggcgag cgtggctccg gcggaggccc ctgcgcacct ttcggaggag aagctctaca aaattgacac cccggcaaac cgtcacgacc ttctcaccgc ggagggcatg gcctgcgctc taaaggtgtt catggggaca atgccgctcc ccaactaccg cgtcctcaac cgtgccaacc cgctctaccg catgaccgtg gagaaaagcg tgaagaacgt gcgcgactac gttgtgtgcg ctgtcctgca caacatccgc ttcaatgagc gcagttacaa cagcttcatc gactaccagg agaagctcca cgccgggttg gcccgccgcc gcacgctggc ctcggttggc acccacgacc tagacaaggt gaacacgacg gactttatct tcgcctgccg cccgcgcgag accatccggt tcgttccgct gcggcagaca agggagctga actgttcagg caacggcctc gcgcagtact acgccgagga ccgccacatc ggcaagtatg taccgctgat ctcctccttt ccgacctacc cggttgtgta cgacggtacc ggccagaacg tgctctcgtt gccgccgatc atcaactcgc gctactcggc catctccgtg gacaccagga acatcttcat tgagtgcacg gccccggacc actacaaggc gcaggtgctg gtgaaccagc tcgtgtgtgc cttctcggcc tactgcgagg atcccttcac agtggaggcg gtgcaggtga actatgagga gccgacgcca gacggaacaa ggtcgcttgt gacaccaacg atggagacga aggaaatgac gatgagcatc gcacgcctca actcgatcat cggcatcgaa atgaaaagtg gtgctgagtg cgcgcagctg ctgaagaaga tgatgtacac gatcaaggag acgacggaga gtgaggtgac ggtgacggtg ccgccaatgc gcacggacgt catcggcgtc gcggacgtga tggaggatgt cgccatcgcg tacggctacg acaacatcgc ctacgtggag tgcaagaccc atggccctgt gacgcagacg ccgctgagca aggtggcgca gctgctgcgc actgagatgg cctgcgcagg ctacacggag ctgctgacgc tgtcactctg ctcgcgcgcc gatgcgttcg agagcctggg ccgcgtggac aacgacgtcg cggcgcacat cgccaacccg cagacgatgg agttccaggt gtgccgccca tcactgctcc ccggtattct gaagacgctc gcgacgaaca agtcgaagcc tctaccgcag cgcttcttcg agtgcgcgga cgtcgtcctg ctggacaacg aacggaattt cccaccggtg ctggagttga aggccgacta cgcgtcctgt ggtgcgcgga accagagaca cctcgccgcc gcgcactgca acggcagctc tagcggattt gagagcattc acggcctcac cgagctcgcc ctactcaagc ttggcgtccc gtcgaaagcg gagatagcag aggactacga aggcgactac tacacactgg agaagggcga ggacggcgcc ttctttcctg gccgcgctat ggacatcatt cttcaccgcg ctggtcaggc agtgcgcatc gggcacctcg gcgtcatcca cccgaacacg ctcaaggcgt acgacattcc gagcccgtgc tcttacatgg agctcaacat ccagttcctg tgcgtccctc tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for LedoB.18754.a.B1.PW38634

PURIFICATION DATe:	6/8/2019
CONCENTRATION:	4.4mg/ml
OBSERVED MW:	60kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1 mM ATP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	21
VIAL VOLUME:	110µl
PERCENT IDENTITY:	86
PERCENT COVERAGE:	49

Protocol Notes

cannot concentrate above ~5 mg/mL, Protein unstable -->precipitation

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCGA CGTTGGCCGT GGCGAAGGAC TACATCTGCC GGCTGATCGG CAAGTCCTAC ACAGACGAGG AGTTCAACGA CCTGTGTTTC CAGTTTGGCC TGGAGCTCGA TGACATTACG AGTGAGAAAG AAATGTTCAT GAGGGAGCAG GGCAAGAGCG CAAAGGCGAG CGTGGCTCCG GCGGAGGCCC CTGCGCACCT TTCGGAGGAG AAGCTCTACA AAATTGACAC CCCGGCAAAC CGTCACGACC TTCTCACCGC GGAGGGCATG GCCTGCGCTC TAAAGGTGTT CATGGGGACA ATGCCGCTCC CCAACTACCG CGTCCTCAAC CGTGCCAACC CGCTCTACCG CATGACCGTG GAGAAAAGCG TGAAGAACGT GCGCGACTAC GTTGTGTGCG CTGTCCTGCA CAACATCCGC TTCAATGAGC GCAGTTACAA CAGCTTCATC GACTACCAGG AGAAGCTCCA CGCCGGGTTG GCCCGCCGCC GCACGCTGGC CTCGGTTGGC ACCCACGACC TAGACAAGGT GAACACGACG GACTTTATCT TCGCCTGCCG CCCGCGCGAG ACCATCCGGT TCGTTCCGCT GCGGCAGACA AGGGAGCTGA ACTGTTCAGG CAACGGCCTC GCGCAGTACT ACGCCGAGGA CCGCCACATC GGCAAGTATG TACCGCTGAT CTCCTCCTTT CCGACCTACC CGGTTGTGTA CGACGGTACC GGCCAGAACG TGCTCTCGTT GCCGCCGATC ATCAACTCGC GCTACTCGGC CATCTCCGTG GACACCAGGA ACATCTTCAT TGAGTGCACG GCCCCGGACC ACTACAAGGC GCAGGTGCTG GTGAACCAGC TCGTGTGTGC CTTCTCGGCC TACTGCGAGG ATCCCTTCAC AGTGGAGGCG GTGCAGGTGA ACTATGAGGA GCCGACGCCA GACGGAACAA GGTCGCTTGT GACACCAACG ATGGAGACGA AGGAAATGAC GATGAGCATC GCACGCCTCA ACTCGATCAT CGGCATCGAA ATGAAAAGTG GTGCTGAGTG CGCGCAGCTG CTGAAGAAGA TGATGTACAC GATCAAGGAG ACGACGGAGA GTGAGGTGAC GGTGACGGTG CCGCCAATGC GCACGGACGT CATCGGCGTC GCGGACGTGA TGGAGGATGT CGCCATCGCG TACGGCTACG ACAACATCGC CTACGTGGAG TGCAAGACCC ATGGCCCTGT GACGCAGACG CCGCTGAGCA AGGTGGCGCA GCTGCTGCGC ACTGAGATGG CCTGCGCAGG CTACACGGAG CTGCTGACGC TGTCACTCTG CTCGCGCGCC GATGCGTTCG AGAGCCTGGG CCGCGTGGAC AACGACGTCG CGGCGCACAT CGCCAACCCG CAGACGATGG AGTTCCAGGT GTGCCGCCCA TCACTGCTCC CCGGTATTCT GAAGACGCTC GCGACGAACA AGTCGAAGCC TCTACCGCAG CGCTTCTTCG AGTGCGCGGA CGTCGTCCTG CTGGACAACG AACGGAATTT CCCACCGGTG CTGGAGTTGA AGGCCGACTA CGCGTCCTGT GGTGCGCGGA ACCAGAGACA CCTCGCCGCC GCGCACTGCA ACGGCAGCTC TAGCGGATTT GAGAGCATTC ACGGCCTCAC CGAGCTCGCC CTACTCAAGC TTGGCGTCCC GTCGAAAGCG GAGATAGCAG AGGACTACGA AGGCGACTAC TACACACTGG AGAAGGGCGA GGACGGCGCC TTCTTTCCTG GCCGCGCTAT GGACATCATT CTTCACCGCG CTGGTCAGGC AGTGCGCATC GGGCACCTCG GCGTCATCCA CCCGAACACG CTCAAGGCGT ACGACATTCC GAGCCCGTGC TCTTACATGG AGCTCAACAT CCAGTTCCTG TGCGTCCCTC TGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT