SSGCID: Target Page

LedoB.18708.a

isoleucyl-tRNA synthetase, putative

CENTER ID:	LedoB.18708.a
ORGANISM:	Leishmania donovani BPK282A1
ASSOCIATED DISEASE:	Leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LedoB.18708.a.B2.GE42678	soluble domain #1		1	607
LedoB.18708.a.B3.GE42679	soluble domain #2		814	1100

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LdBPK_365870.1
UniProt:	E9BUU4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAGEGQQQQQ QDPPAGQQGV PVTNYPDVLD FSKMEEEVLA MWREKDCFRT SMKLSEGRQP FSFYDGPPFA TGLPHYGHIL AGTIKDMVTR FAYQTNHHVI RRFGWDCHGL PVEYEIDKML GIKTSHDVAK LGIDKYNDEC KKIVTRYVDE WRKTVERVGR WIDFDNDYKT MHLTYMESIW WVFSQLWEKK MVYRGFRVMP YSTACTTPLS NFEANSNYKE VSDLAVTVAF QARADPNTYF LAWTTTPWTL PSNLSLCVHP ELDYVKVLDN KSKRHYWLAE ARLAEVYPKK DSKKASKAKD VDNAAEADAA PYTVVEKVKG AQLVGEKYVP LFPYFESSMS ATAFRVISGT YVTTDAGTGI VHQAPAFGED DYQACLDAGI FEKGGKFVCP VDENGMFTSE VTDFAGKYVK AADPEIIKAL ETKGHLFHKA SIVHSYPFCW RSDTPLIYKA VESWFVNVEA FRDRLIECND KTYWVPDFVK TRRFSNWLAE ARDWNVSRNR YWGTPMPVWH SEDWEEVVCI SSIKQLEELS GVTGITDIHR QFVDDITIPS KRPGMPPLKR VPMVFDCWFE SGSMPYAQEH FPFENQEKFK SLFPADFVSE GLDQTRGWFY TLLVLGVALF DVSPFRNVAV SGLVLAEDGK KMSKRLKNYP EPKVVIDTYG ADALRMYMIS SPVVRAEPLR FREAGVKAVV KDILLPLFNA AKFFISNTNY CTAAGGQVSL QVRSTNEMDR WILASCQSLL RYVKAEMRLY HLYNVVPGIL RFVGDLSNWY VRMNRRRMKN ATDSEDRSQA LSTMLYLLFS VSRIVGHIAP FVAEMLYQQI KPLLPANEQV DSVHYLMIPD EDTSLDDPEL ERAMSRMMNI VDLVRVLRDQ MVIPIKRPVR QVVIVHPDQQ YIDDVRKVAG YIKDEVNAFE IVMSSGENYL ETKLDANFEV LGKKYRKEMP AIRKGIQAMT QNEVAKFLHD KKGVVVGKEL TIEDVKVLRQ VKEGIADFQS NTDNDVVVLV DKRQDQELID SWRAREFVNR VQQLRKKARL VVTDEVDVYF EAEEADLTAS ILHSKDQINQ TLRGVWTTMD QKPSDAELVA EEDNNISGVA VRLVFTKPKA

NT Sequence

atggcgggcg aaggtcagca gcagcagcag caagaccccc cagcggggca gcagggcgta ccggtgacga actatcccga tgtgctcgac ttctcgaaga tggaggagga ggtgctggcg atgtggcggg agaaggattg cttccgcact tccatgaagc tctccgaggg ccgccagccg ttcagcttct acgacggccc accgttcgcg actggcctgc cgcactacgg ccacatcttg gctggcacca tcaaggacat ggtcactcgc ttcgcctacc agacgaacca ccacgtcatc cgtcgcttcg gctgggactg ccacggtctg ccggtggagt acgagatcga caagatgctc ggcatcaaga cgtctcacga cgtggcgaag ctcggcattg acaagtataa cgacgagtgc aagaagatcg tcacccgcta cgtggacgaa tggaggaaga ccgtcgagcg cgttggccgc tggatcgact ttgacaacga ctacaagacg atgcacctca catacatgga gagtatctgg tgggttttct cgcagctgtg ggagaagaag atggtgtacc gcggctttcg cgtgatgccg tactcgaccg cctgcacgac gccgctgagc aacttcgagg ctaactccaa ctacaaggaa gtcagcgacc tcgccgtgac ggtcgccttc caggcccgcg ccgaccccaa cacatacttc ctggcctgga ccaccacgcc gtggacgttg ccgagcaacc tcagtttgtg cgtgcacccc gagctggact acgtgaaggt gctggacaac aagtcgaagc gacactactg gctcgcagag gcccgcctgg cggaggtgta cccgaagaag gacagcaaga aggcctccaa ggcgaaggac gtggacaacg ccgcggaggc cgatgccgcg ccgtacacgg tcgtcgagaa agtcaagggc gcgcagctag tgggcgagaa gtacgtgcca cttttcccgt actttgagtc gtccatgagc gccacggcct tccgcgtcat ctccggtacc tacgtgacga cagacgctgg tacaggtatc gtgcaccagg cccccgcttt cggtgaggat gattaccagg cgtgcctcga cgccggcatc ttcgagaagg gcggcaagtt cgtctgcccg gtcgacgaga acggcatgtt cacgagcgag gtgacggatt tcgccggcaa gtacgtcaag gcggcggatc cggagattat caaagcgctc gagacgaaag ggcacctctt tcacaaggcg tcgattgtgc acagctaccc cttctgctgg cgcagcgaca cccccctcat ctacaaggct gtcgagtcgt ggtttgtcaa cgtcgaggcc ttccgcgacc gcctcatcga gtgcaacgac aagacgtact gggtacctga ttttgtcaag acccgccgct tctccaactg gctcgccgag gcgcgtgact ggaacgtgtc acgcaaccgt tactggggca cgccgatgcc ggtctggcac agcgaggact gggaggaggt tgtgtgcatc agcagcatca agcagctcga ggagctctcc ggcgtcaccg gcatcaccga catccaccgc cagttcgtcg acgatatcac gatcccaagt aagcggcctg gcatgccgcc gctgaagcgt gtgccgatgg tgtttgactg ctggttcgag tccggctcga tgccgtacgc ccaggagcac ttccctttcg agaaccagga gaagttcaag agcctgtttc cggccgactt tgtgtcggag ggcttggacc agacgcgcgg ctggttctac acccttctcg tgctcggcgt tgccctcttc gacgtctccc ctttccgcaa cgtcgccgtc agcgggctcg tgctcgccga ggacggtaag aagatgagca agcgcctcaa gaactaccct gagccgaagg tggtgatcga cacgtacggc gcggatgccc tgcgcatgta catgattagc tccccggttg tgcgcgccga gccgctgcgc ttccgcgaag caggtgtgaa ggccgtggtc aaggacatcc ttctgcctct gttcaacgcc gcgaagttct tcatctcgaa cacaaactac tgcactgcgg ccggtggtca ggtgtcgctg caggttcgca gcacaaacga gatggaccgc tggattctcg cctcgtgcca gagtctgctg cgctacgtga aggcggagat gcggctgtac cacctgtaca acgtcgtccc cggcattctg cgtttcgtcg gtgacctgtc caactggtac gtgcgcatga accgccgccg catgaagaac gccacggaca gcgaggaccg ctcgcaggcg ctgtcgacga tgctgtacct cctcttctcg gtgtcccgca ttgtgggcca catcgccccc tttgtggcag aaatgcttta ccagcagatc aagcccctcc tcccggcgaa cgagcaagtg gactccgtgc actacctcat gatccccgac gaggatacct ccttggacga cccggagctg gagcgcgcca tgtcccgcat gatgaacatt gtggatttgg tgcgtgtgtt gcgcgatcag atggtcattc cgatcaagcg gccggtgcgt caggttgtca ttgttcaccc ggaccagcag tacatcgacg acgtgcgcaa ggtggcgggc tacatcaagg atgaggtgaa cgcgttcgag attgtgatgt cgagtggcga gaattacctg gagacgaagc tggacgcaaa cttcgaggtg cttggcaaga agtaccgcaa ggagatgccc gcgatccgca agggtatcca ggccatgacg cagaatgagg tggccaagtt cctgcacgac aagaagggcg tcgtcgtcgg caaggagctg accatcgagg acgtgaaggt gctgcgccag gtaaaggagg gcattgccga cttccagagc aacactgaca acgacgtcgt tgtgctggtt gacaagcgcc aggatcagga actgattgac tcgtggcgcg cgcgtgagtt cgtgaaccgc gtacagcagc tgcgtaagaa ggcgaggcta gtggtgacgg acgaggtgga tgtgtacttt gaggcggagg aggcggatct gacggcgtcc atcctccact ctaaggacca gattaatcag acactgaggg gcgtgtggac gacgatggac cagaagccta gcgacgccga gctcgtcgcg gaggaggaca ataacatctc gggggtggcg gtgcgcctcg tcttcaccaa gcccaaggct taa

Details for LedoB.18708.a.B2.GE42678

HARVESTED ON:	9/28/2018
SEQUENCED ON:	10/17/2018
EXPECTED MW:	71kDa
OBSERVED MW:	71kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMA GEGQQQQQQD PPAGQQGVPV TNYPDVLDFS KMEEEVLAMW REKDCFRTSM KLSEGRQPFS FYDGPPFATG LPHYGHILAG TIKDMVTRFA YQTNHHVIRR FGWDCHGLPV EYEIDKMLGI KTSHDVAKLG IDKYNDECKK IVTRYVDEWR KTVERVGRWI DFDNDYKTMH LTYMESIWWV FSQLWEKKMV YRGFRVMPYS TACTTPLSNF EANSNYKEVS DLAVTVAFQA RADPNTYFLA WTTTPWTLPS NLSLCVHPEL DYVKVLDNKS KRHYWLAEAR LAEVYPKKDS KKASKAKDVD NAAEADAAPY TVVEKVKGAQ LVGEKYVPLF PYFESSMSAT AFRVISGTYV TTDAGTGIVH QAPAFGEDDY QACLDAGIFE KGGKFVCPVD ENGMFTSEVT DFAGKYVKAA DPEIIKALET KGHLFHKASI VHSYPFCWRS DTPLIYKAVE SWFVNVEAFR DRLIECNDKT YWVPDFVKTR RFSNWLAEAR DWNVSRNRYW GTPMPVWHSE DWEEVVCISS IKQLEELSGV TGITDIHRQF VDDITIPSKR PGMPPLKRVP MVFDCWFESG SMPYAQEHFP FENQEKFKSL FPADFVSEGL DQTRG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg gcgaaggtca gcagcagcag cagcaagacc ccccagcggg gcagcagggc gtaccggtga cgaactatcc cgatgtgctc gacttctcga agatggagga ggaggtgctg gcgatgtggc gggagaagga ttgcttccgc acttccatga agctctccga gggccgccag ccgttcagct tctacgacgg cccaccgttc gcgactggcc tgccgcacta cggccacatc ttggctggca ccatcaagga catggtcact cgcttcgcct accagacgaa ccaccacgtc atccgtcgct tcggctggga ctgccacggt ctgccggtgg agtacgagat cgacaagatg ctcggcatca agacgtctca cgacgtggcg aagctcggca ttgacaagta taacgacgag tgcaagaaga tcgtcacccg ctacgtggac gaatggagga agaccgtcga gcgcgttggc cgctggatcg actttgacaa cgactacaag acgatgcacc tcacatacat ggagagtatc tggtgggttt tctcgcagct gtgggagaag aagatggtgt accgcggctt tcgcgtgatg ccgtactcga ccgcctgcac gacgccgctg agcaacttcg aggctaactc caactacaag gaagtcagcg acctcgccgt gacggtcgcc ttccaggccc gcgccgaccc caacacatac ttcctggcct ggaccaccac gccgtggacg ttgccgagca acctcagttt gtgcgtgcac cccgagctgg actacgtgaa ggtgctggac aacaagtcga agcgacacta ctggctcgca gaggcccgcc tggcggaggt gtacccgaag aaggacagca agaaggcctc caaggcgaag gacgtggaca acgccgcgga ggccgatgcc gcgccgtaca cggtcgtcga gaaagtcaag ggcgcgcagc tagtgggcga gaagtacgtg ccacttttcc cgtactttga gtcgtccatg agcgccacgg ccttccgcgt catctccggt acctacgtga cgacagacgc tggtacaggt atcgtgcacc aggcccccgc tttcggtgag gatgattacc aggcgtgcct cgacgccggc atcttcgaga agggcggcaa gttcgtctgc ccggtcgacg agaacggcat gttcacgagc gaggtgacgg atttcgccgg caagtacgtc aaggcggcgg atccggagat tatcaaagcg ctcgagacga aagggcacct ctttcacaag gcgtcgattg tgcacagcta ccccttctgc tggcgcagcg acacccccct catctacaag gctgtcgagt cgtggtttgt caacgtcgag gccttccgcg accgcctcat cgagtgcaac gacaagacgt actgggtacc tgattttgtc aagacccgcc gcttctccaa ctggctcgcc gaggcgcgtg actggaacgt gtcacgcaac cgttactggg gcacgccgat gccggtctgg cacagcgagg actgggagga ggttgtgtgc atcagcagca tcaagcagct cgaggagctc tccggcgtca ccggcatcac cgacatccac cgccagttcg tcgacgatat cacgatccca agtaagcggc ctggcatgcc gccgctgaag cgtgtgccga tggtgtttga ctgctggttc gagtccggct cgatgccgta cgcccaggag cacttccctt tcgagaacca ggagaagttc aagagcctgt ttccggccga ctttgtgtcg gagggcttgg accagacgcg cggctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for LedoB.18708.a.B3.GE42679

HARVESTED ON:	data unavailable
SEQUENCED ON:	10/17/2018
EXPECTED MW:	34kDa
OBSERVED MW:	71kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHEM LYQQIKPLLP ANEQVDSVHY LMIPDEDTSL DDPELERAMS RMMNIVDLVR VLRDQMVIPI KRPVRQVVIV HPDQQYIDDV RKVAGYIKDE VNAFEIVMSS GENYLETKLD ANFEVLGKKY RKEMPAIRKG IQAMTQNEVA KFLHDKKGVV VGKELTIEDV KVLRQVKEGI ADFQSNTDND VVVLVDKRQD QELIDSWRAR EFVNRVQQLR KKARLVVTDE VDVYFEAEEA DLTASILHSK DQINQTLRGV WTTMDQKPSD AELVAEEDNN ISGVAVRLVF TKPKA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaaatgc tttaccagca gatcaagccc ctcctcccgg cgaacgagca agtggactcc gtgcactacc tcatgatccc cgacgaggat acctccttgg acgacccgga gctggagcgc gccatgtccc gcatgatgaa cattgtggat ttggtgcgtg tgttgcgcga tcagatggtc attccgatca agcggccggt gcgtcaggtt gtcattgttc acccggacca gcagtacatc gacgacgtgc gcaaggtggc gggctacatc aaggatgagg tgaacgcgtt cgagattgtg atgtcgagtg gcgagaatta cctggagacg aagctggacg caaacttcga ggtgcttggc aagaagtacc gcaaggagat gcccgcgatc cgcaagggta tccaggccat gacgcagaat gaggtggcca agttcctgca cgacaagaag ggcgtcgtcg tcggcaagga gctgaccatc gaggacgtga aggtgctgcg ccaggtaaag gagggcattg ccgacttcca gagcaacact gacaacgacg tcgttgtgct ggttgacaag cgccaggatc aggaactgat tgactcgtgg cgcgcgcgtg agttcgtgaa ccgcgtacag cagctgcgta agaaggcgag gctagtggtg acggacgagg tggatgtgta ctttgaggcg gaggaggcgg atctgacggc gtccatcctc cactctaagg accagattaa tcagacactg aggggcgtgt ggacgacgat ggaccagaag cctagcgacg ccgagctcgt cgcggaggag gacaataaca tctcgggggt ggcggtgcgc ctcgtcttca ccaagcccaa ggcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat