LedoB.18681.a

prolyl-tRNA synthetase, putative (fragment)

CENTER ID: LedoB.18681.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.18681.a.B2.GE42676 soluble domain 24 361
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LdBPK_181220.1
UniProt: E9BDV2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRGGRLLGPP HACFSLLSNS VSCLSSSRAS HRSITRNLGV RHHLPSSSPR TPSYSTSPVT PVTPTRGYSG TKIHTQNKSP PASSLPHMSI EDCRGFHEVK ALLQELGLDI PIVHHDEKAT VEEVLDLLHK MGIHAAGTKT LFLKSKKGEL VMATAFKSTP TDLKFIQKVT NTKDLRFASS EVLQECLAVV QGCVTPFALI NNIEKRPITL LIDRSLEAST LPLAFCLCRN DYTAVITFEE LKKYLEKLGH AYKLVDFGAA SADAAVTGGA AAPAPKPKKA PADAAAAAAA PASAQSGETK LGIAAKREEN FSAWYIDVIT KAEMIEYYDV SGCYIIRPWA YYVWKCVQRF LGGKIEKLGV E
NT Sequence
atgcgtggtg gacggctctt gggcccacct cacgcgtgct tttctctgtt aagtaactct gtgtcttgcc tatcctccag tcgggcttcg catcgatcga tcacgcgcaa cttgggcgta cgacatcacc tgccgtcgtc ttcgcctcga acgccgagtt actcgacaag tcccgtgacc cctgtgacgc cgacgcgcgg ttacagtggt actaagatac acacgcaaaa taaatcgcca ccagcttctt ctctgcctca catgtccatc gaggactgcc gcggctttca tgaggtaaag gcccttctcc aagagctggg actcgacatt ccgatcgtgc accatgacga gaaggccacc gtcgaggagg tgctggacct cctgcacaaa atgggcatcc acgctgcggg caccaagacg ctcttcctca agagcaagaa gggcgaactg gtgatggcga cggcgttcaa gtccaccccg acggacctca agttcattca gaaggtcacc aacaccaagg acctccgctt tgcttccagc gaggtgctgc aggagtgcct cgccgtcgtg cagggctgcg tcacaccgtt tgccctcatc aacaatatcg agaagcgtcc cattacgctc ctcatagacc gctccctgga ggccagcacg ctgccgctcg ccttctgcct gtgccgtaac gattacactg ccgtcataac cttcgaggag ctcaagaagt acctcgagaa gctcgggcat gcgtacaagc tggtggactt tggtgcggct tcggcggacg ctgcggtgac gggcggcgct gctgctcctg ctccgaagcc gaagaaggcg cccgcggacg ctgctgctgc tgctgctgcg cctgcgtctg cacagtctgg cgagacgaag ctgggcatcg cggcgaagcg cgaggagaac ttctctgcgt ggtacattga tgtgatcacg aaggcggaga tgatcgagta ctacgacgtg tccgggtgct acattattcg tccgtgggcg tactacgtgt ggaagtgcgt gcagcgcttc cttggtggga agatcgagaa gcttggcgtg gag
Details for LedoB.18681.a.B2.GE42676
HARVESTED ON: 9/28/2018
SEQUENCED ON: 10/17/2018
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHML SSSRASHRSI TRNLGVRHHL PSSSPRTPSY STSPVTPVTP TRGYSGTKIH TQNKSPPASS LPHMSIEDCR GFHEVKALLQ ELGLDIPIVH HDEKATVEEV LDLLHKMGIH AAGTKTLFLK SKKGELVMAT AFKSTPTDLK FIQKVTNTKD LRFASSEVLQ ECLAVVQGCV TPFALINNIE KRPTTLLIDR SLEASTLPLA FCLCRNDYTA VITFEELKKY LEKLGHAYKL VDFGAASADA AVMGGAAAPA PKPKKAPADA AAAAAAPASA QSGETKLGIA AKREENFSAW YIDVITKAEM IEYYDVSGCY IIRPWAYYVW KCVQRFLGG
Validated NT Sequence
tcccaccaag gaagcgctgc acgcacttcc acacgtagta cgcccacgga cgaataatgt agcacccgga cacgtcgtag tactcgatca tctccgcctt cgtgatcaca tcaatgtacc acgcagagaa gttctcctcg cgcttcgccg cgatgcccag cttcgtctcg ccagactgtg cagacgcagg cgcagcagca gcagcagcag cgtccgcggg cgccttcttc ggcttcggag caggagcagc agcgccgccc atcaccgcag cgtccgccga agccgcacca aagtccacca gcttgtacgc atgcccgagc ttctcgaggt acttcttgag ctcctcgaag gttatgacgg cagtgtaatc gttacggcac aggcagaagg cgagcggcag cgtgctggcc tccagggagc ggtctatgag gagcgtagtg ggacgcttct cgatattgtt gatgagggca aacggtgtga cgcagccctg cacgacggcg aggcactcct gcagcacctc gctggaagca aagcggaggt ccttggtgtt ggtgaccttc tgaatgaact tgaggtccgt cggggtggac ttgaacgccg tcgccatcac cagttcgccc ttcttgctct tgaggaagag cgtcttggtg cccgcagcgt ggatgcccat tttgtgcagg aggtccagca cctcctcgac ggtggccttc tcgtcatggt gcacgatcgg aatgtctagt cccagctctt ggagaagggc ctttacctca tgaaagccgc ggcagtcctc gatggacatg tgaggcagag aagaagctgg tggcgattta ttttgcgtgt gtatcttagt accactgtaa ccgcgcgtcg gcgtcacagg ggtcacggga cttgtcgagt aactcggcgt tcgaggcgaa gacgacggca ggtgatgtcg tacgcccaag ttgcgcgtga tcgatcgatg cgaagcccga ctggaggata gcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHLS SSRASHRSIT RNLGVRHHLP SSSPRTPSYS TSPVTPVTPT RGYSGTKIHT QNKSPPASSL PHMSIEDCRG FHEVKALLQE LGLDIPIVHH DEKATVEEVL DLLHKMGIHA AGTKTLFLKS KKGELVMATA FKSTPTDLKF IQKVTNTKDL RFASSEVLQE CLAVVQGCVT PFALINNIEK RPITLLIDRS LEASTLPLAF CLCRNDYTAV ITFEELKKYL EKLGHAYKLV DFGAASADAA VTGGAAAPAP KPKKAPADAA AAAAAPASAQ SGETKLGIAA KREENFSAWY IDVITKAEMI EYYDVSGCYI IRPWAYYVWK CVQRFLGGKI EKLGVE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctatcct ccagtcgggc ttcgcatcga tcgatcacgc gcaacttggg cgtacgacat cacctgccgt cgtcttcgcc tcgaacgccg agttactcga caagtcccgt gacccctgtg acgccgacgc gcggttacag tggtactaag atacacacgc aaaataaatc gccaccagct tcttctctgc ctcacatgtc catcgaggac tgccgcggct ttcatgaggt aaaggccctt ctccaagagc tgggactcga cattccgatc gtgcaccatg acgagaaggc caccgtcgag gaggtgctgg acctcctgca caaaatgggc atccacgctg cgggcaccaa gacgctcttc ctcaagagca agaagggcga actggtgatg gcgacggcgt tcaagtccac cccgacggac ctcaagttca ttcagaaggt caccaacacc aaggacctcc gctttgcttc cagcgaggtg ctgcaggagt gcctcgccgt cgtgcagggc tgcgtcacac cgtttgccct catcaacaat atcgagaagc gtcccattac gctcctcata gaccgctccc tggaggccag cacgctgccg ctcgccttct gcctgtgccg taacgattac actgccgtca taaccttcga ggagctcaag aagtacctcg agaagctcgg gcatgcgtac aagctggtgg actttggtgc ggcttcggcg gacgctgcgg tgacgggcgg cgctgctgct cctgctccga agccgaagaa ggcgcccgcg gacgctgctg ctgctgctgc tgcgcctgcg tctgcacagt ctggcgagac gaagctgggc atcgcggcga agcgcgagga gaacttctct gcgtggtaca ttgatgtgat cacgaaggcg gagatgatcg agtactacga cgtgtccggg tgctacatta ttcgtccgtg ggcgtactac gtgtggaagt gcgtgcagcg cttccttggt gggaagatcg agaagcttgg cgtggagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at