SSGCID: Target Page

LedoB.18445.a

tyrosyl or methionyl-tRNA synthetase-like protein

CENTER ID:	LedoB.18445.a
ORGANISM:	Leishmania donovani BPK282A1
ASSOCIATED DISEASE:	Leishmaniasis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LedoB.18445.a.B1.GE42675	full length		1	237

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LdBPK_161170.1
UniProt:	E9BCX1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MASHAEGRLL RRSVPYVESW IAELTPKPVA SAAGAAPAPK GKAKGGAAST GTENATAMSR CCFAVGKVLE VSRHPESEKL YIEKIDLGAE LNMLSNNEPR TILSGLQEFV KEEDFVNRLV LVIANLEPRK IGGIPSAGMV LCASTGEDPH DPALAGQGER KVVLLDIPEG TAVGERVVFE GHDMPYEPVL KKKLAKNFEE VMKDVCSSAD GVVCWQGKPF QTSAGVIKAS LCNARIS

NT Sequence

atggcctcgc atgctgaggg acgtctgctg cgccgctcgg tgccctacgt ggagtcgtgg atcgcggagc tgacccccaa gcctgtagcc tctgccgctg gtgccgctcc tgctccgaag ggcaaggcga agggtggcgc tgcgtcgact ggcactgaga acgccacggc catgtcgcgg tgctgcttcg cggtcggcaa ggtgctggag gtgtcacgcc acccggagag cgagaagctg tacatcgaga agattgacct cggcgcggag ttgaacatgc tgagcaacaa cgagccgcgc accatcctca gtggattgca ggagttcgta aaggaggaag acttcgtgaa ccgcctcgtg ctcgtgattg cgaacctgga accgcgcaag attggcggca ttccgtccgc tggcatggtg ctgtgcgcct ccaccggcga ggacccgcac gaccccgcgt tggctggcca gggggagcgc aaggtggtgc tgctggacat cccggagggg acggccgtcg gggagcgcgt agtgttcgag gggcacgaca tgccgtacga gccggttctg aagaaaaagc ttgccaagaa ctttgaggag gtcatgaagg acgtgtgcag cagcgccgac ggcgtggtgt gctggcaggg gaagcctttc cagacctcgg ccggcgtcat caaggcatcc ctgtgcaacg ctcgtatctc gtaa

Details for LedoB.18445.a.B1.GE42675

HARVESTED ON:	9/28/2018
SEQUENCED ON:	10/17/2018
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA SHAEGRLLRR SVPYVESWIA ELTPKPVASA AGAAPAPKGK AKGGAASTGT ENATAMSRCC FAVGKVLEVS RHPESEKLYI EKIDLGAELN MLSNNEPRTI LSGLQEFVKE EDFVNRLVLV IANLEPRKIG GIPSAGMVLC ASTGEDPHDP ALAGQGERKV VLLDIPEGTA VGERVVFEGH DMPYEPVLKK KLAKNFEEVM KDVCSSADGV VCWQGKPFQT SAGVIKASLC NARIS

Validated NT Sequence

atggctcacc accaccacca ccatatggcc tcgcatgctg agggacgtct gctgcgccgc tcggtgccct acgtggagtc gtggatcgcg gagctgaccc ccaagcctgt agcctctgcc gctggtgccg ctcctgctcc gaagggcaag gcgaagggtg gcgctgcgtc gactggcact gagaacgcca cggccatgtc gcggtgctgc ttcgcggtcg gcaaggtgct ggaggtgtca cgccacccgg agagcgagaa gctgtacatc gagaagattg acctcggcgc ggagttgaac atgctgagca acaacgagcc gcgcaccatc ctcagtggat tgcaggagtt cgtaaaggag gaagacttcg tgaaccgcct cgtgctcgtg attgcgaacc tggaaccgcg caagattggc ggcattccgt ccgctggcat ggtgctgtgc gcctccaccg gcgaggaccc gcacgacccc gcgttggctg gccaggggga gcgcaaggtg gtgctgctgg acatcccgga ggggacggcc gtcggggagc gcgtagtgtt cgaggggcac gacatgccgt acgagccggt tctgaagaaa aagcttgcca agaactttga ggaggtcatg aaggacgtgt gcagcagcgc cgacggcgtg gtgtgctggc aggggaagcc tttccagacc tcggccggcg tcatcaaggc atccctgtgc aacgctcgta tctcgtaagt gagtaagat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcct cgcatgctga gggacgtctg ctgcgccgct cggtgcccta cgtggagtcg tggatcgcgg agctgacccc caagcctgta gcctctgccg ctggtgccgc tcctgctccg aagggcaagg cgaagggtgg cgctgcgtcg actggcactg agaacgccac ggccatgtcg cggtgctgct tcgcggtcgg caaggtgctg gaggtgtcac gccacccgga gagcgagaag ctgtacatcg agaagattga cctcggcgcg gagttgaaca tgctgagcaa caacgagccg cgcaccatcc tcagtggatt gcaggagttc gtaaaggagg aagacttcgt gaaccgcctc gtgctcgtga ttgcgaacct ggaaccgcgc aagattggcg gcattccgtc cgctggcatg gtgctgtgcg cctccaccgg cgaggacccg cacgaccccg cgttggctgg ccagggggag cgcaaggtgg tgctgctgga catcccggag gggacggccg tcggggagcg cgtagtgttc gaggggcacg acatgccgta cgagccggtt ctgaagaaaa agcttgccaa gaactttgag gaggtcatga aggacgtgtg cagcagcgcc gacggcgtgg tgtgctggca ggggaagcct ttccagacct cggccggcgt catcaaggca tccctgtgca acgctcgtat ctcgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat