SSGCID: Target Page

LedoB.18236.a

KREL2

CENTER ID:	LedoB.18236.a
ORGANISM:	Leishmania donovani BPK282A1
ASSOCIATED DISEASE:	Leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LedoB.18236.a.B1.GE41118	full length		1	418

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	LdBPK_201700.1
RefSeq:	XP_003860510.1
UniProt:	E9BEW8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFRRCLLTRL VRRSLVLFSD EKEALFERYS EIENAHSRRI EALKDAGLFN DEWIATEKVH GANFGIYSTE HGKTIQYAKR SGIMPPYEHF FGYHILIPDL VRYVKQGREL LTEQLGMSPS TVIINGELFG GKYDHPSVPK RRQNVMVAGR PRTITAVQNN AFPQYCPDLH FYAFDIKYRR TEEEEYCTMT YDQALAIFEK ISGLLYAKAI IRGPLSKVAA FDVENFVTTI PPLVGMGDYP LKGNWAEGLV VKHCRRGEPG FDAKGLTIMK FKCTAFQEIS TDRRQGPRVD ALEEVRRQSI KVSGVQLPDM ESVIRDPEVR QATHHLLDHV CENRLQSVLS KIGTGPFETQ SMTPTELSTL LAKDALKDFL KEADAKIVNT PLLVRREMAR YVLFEARRYV ASRWKDVVGQ QREADGES

NT Sequence

atgtttcggc gatgcctgct tacacggctc gtgcgtcgct cgctggtcct cttttcggat gaaaaagaag cacttttcga gcggtatagc gagattgaaa acgcccactc gcgacgcatt gaagcactga aggacgcggg tctcttcaat gatgagtgga ttgccacgga gaaggtgcat ggcgccaact tcggcatcta ctcaacagag catgggaaga ctattcagta cgcgaagcgg agcggcatca tgccaccgta cgagcacttt ttcggctacc acatcctcat acctgatctc gtgaggtatg tcaagcaagg gcgagaactg cttacggaac agctcggcat gtcgcccagc accgtaatca tcaacggtga gctctttggt ggcaagtacg accatccgag cgtgccgaag cggcggcaga acgtgatggt ggccggcagg ccacgcacca tcacggctgt gcagaacaat gcctttcctc agtactgccc agacctgcat ttctacgcct ttgacatcaa gtaccggagg accgaagagg aggagtactg caccatgaca tacgaccaag ccttggccat cttcgagaaa atttcgggcc ttctctacgc gaaggctatc attcgagggc cgctgagcaa ggtggccgcg ttcgacgtgg aaaacttcgt cacgacaatt ccgccgctgg tgggcatggg cgactacccg ctcaagggga actgggcgga gggcctcgtt gtaaaacact gccgtcgcgg cgagcccggc ttcgacgcaa agggcctcac catcatgaag ttcaagtgca ccgccttcca ggaaatctcg accgatcgcc gccagggacc gcgcgtagac gcgctggagg aggttcggcg gcagtccata aaagtctccg gcgtacagct gccggacatg gagagcgtca ttcgcgaccc cgaggtgcgc caagcgacac atcacctgct cgaccacgtg tgtgagaatc gcctccagag tgtcttgtcc aagatcggta caggcccgtt cgagacgcag tccatgaccc ccacagagct ctccacgctg ctagcaaagg atgcgctgaa ggacttcttg aaggaggcgg atgcaaagat cgtcaacacg ccgctactcg tgcgccgcga gatggcccgc tacgttcttt ttgaggcgcg caggtacgta gccagtagat ggaaggacgt cgttgggcag caaagggagg cagacgggga gtcgtga

Details for LedoB.18236.a.B1.GE41118

HARVESTED ON:	2/8/2017
SEQUENCED ON:	2/13/2017
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMF RRCLLTRLVR RSLVLFSDEK EALFERYSEI ENAHSRRIEA LKDAGLFNDE WIATEKVHGA NFGIYSTEHG KTIQYAKRSG IMPPYEHFFG YHILIPDLVR YAKQGRELLT EQLGMSPSTV IINGELFGGK YDHPSVPKRR QNVMVAGRPR TITAVQNNAF PQYCPDLHFY AFDIKYRRTE EEEYCTMTYD QALAIFEKIS GLLYAKAIIR GPLSKVAAFD VENFVTTIPP LVGMGDYPLK GNWAEGLVVK HCRRGEPGFD AKGLTIMKFK CTAFQEISTD RRQGPRVDAL EEVRRQSIKV SGVQLPDMES VIRDPEVRQA THHLLDHVCE NRLQSVLSKI GTGPFETQSM TPTELSTLXA KDAXKDFLKE ADAKIVXTPL LVRREMARYV XXXARRYVAS RWKDVVGQQ

Validated NT Sequence

tttgctgccc aacgacgtcc ttccatctac tggctacgta cctgcgcgcc tnnnnnngaa cgtagcgggc catctcgcgg cgcacgagta gcggcgtgtn gacgatcttt gcatccgcct ccttcaagaa gtccttcnnc gcatcctttg ctngcagcgt ggagagctct gtgggggtca tggactgcgt ctcgaacggg cctgtaccga tcttggacaa gacactctgg aggcgattct cacacacgtg gtcgagcagg tgatgtgtcg cttggcgcac ctcggggtcg cgaatgacgc tctccatgtc cggcagctgt acgccggaga cttttatgga ctgccgccga acctcctcca gcgcgtctac gcgcggtccc tggcggcgat cggtcgagat ttcctggaag gcagtgcact tgaacttcat gatggtgagg ccctttgcgt cgaagccggg ctcgccgcga cggcagtgtt ttacaacgag gccctccgcc cagttcccct tgagcgggta gtcgcccatg cccaccagcg gcggaattgt cgtgacgaag ttttccacgt cgaacgcggc caccttgctc agcggccctc gaatgatagc cttcgcgtag agaaggcccg aaattttctc gaagatggcc aaggcttggt cgtatgtcat ggtgcagtac tcctcctctt cggtcctccg gtacttgatg tcaaaggcgt agaaatgcag gtctgggcag tactgaggaa aggcattgtt ctgcacagcc gtgatggtgc gtggcctgcc ggccaccatc acgttctgcc gccgcttcgg cacgctcgga tggtcgtact tgccaccaaa gagctcaccg ttgatgatta cggtgctggg cgacatgccg agctgttccg taagcagttc tcgcccttgc ttggcatacc tcacgagatc aggtatgagg atgtggtagc cgaaaaagtg ctcgtacggt ggcatgatgc cgctccgctt cgcgtactga atagtcttcc catgctctgt tgagtagatg ccgaagttgg cgccatgcac cttctccgtg gcaatccact catcattgaa gagacccgcg tccttcagtg cttcaatgcg tcgcgagtgg gcgttttcaa tctcgctata ccgctcgaaa agtgcttctt tttcatccga aaagaggacc agcgagcgac gcacgagccg tgtaagcagg catcgccgaa acatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHMF RRCLLTRLVR RSLVLFSDEK EALFERYSEI ENAHSRRIEA LKDAGLFNDE WIATEKVHGA NFGIYSTEHG KTIQYAKRSG IMPPYEHFFG YHILIPDLVR YVKQGRELLT EQLGMSPSTV IINGELFGGK YDHPSVPKRR QNVMVAGRPR TITAVQNNAF PQYCPDLHFY AFDIKYRRTE EEEYCTMTYD QALAIFEKIS GLLYAKAIIR GPLSKVAAFD VENFVTTIPP LVGMGDYPLK GNWAEGLVVK HCRRGEPGFD AKGLTIMKFK CTAFQEISTD RRQGPRVDAL EEVRRQSIKV SGVQLPDMES VIRDPEVRQA THHLLDHVCE NRLQSVLSKI GTGPFETQSM TPTELSTLLA KDALKDFLKE ADAKIVNTPL LVRREMARYV LFEARRYVAS RWKDVVGQQR EADGES

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttc ggcgatgcct gcttacacgg ctcgtgcgtc gctcgctggt cctcttttcg gatgaaaaag aagcactttt cgagcggtat agcgagattg aaaacgccca ctcgcgacgc attgaagcac tgaaggacgc gggtctcttc aatgatgagt ggattgccac ggagaaggtg catggcgcca acttcggcat ctactcaaca gagcatggga agactattca gtacgcgaag cggagcggca tcatgccacc gtacgagcac tttttcggct accacatcct catacctgat ctcgtgaggt atgtcaagca agggcgagaa ctgcttacgg aacagctcgg catgtcgccc agcaccgtaa tcatcaacgg tgagctcttt ggtggcaagt acgaccatcc gagcgtgccg aagcggcggc agaacgtgat ggtggccggc aggccacgca ccatcacggc tgtgcagaac aatgcctttc ctcagtactg cccagacctg catttctacg cctttgacat caagtaccgg aggaccgaag aggaggagta ctgcaccatg acatacgacc aagccttggc catcttcgag aaaatttcgg gccttctcta cgcgaaggct atcattcgag ggccgctgag caaggtggcc gcgttcgacg tggaaaactt cgtcacgaca attccgccgc tggtgggcat gggcgactac ccgctcaagg ggaactgggc ggagggcctc gttgtaaaac actgccgtcg cggcgagccc ggcttcgacg caaagggcct caccatcatg aagttcaagt gcaccgcctt ccaggaaatc tcgaccgatc gccgccaggg accgcgcgta gacgcgctgg aggaggttcg gcggcagtcc ataaaagtct ccggcgtaca gctgccggac atggagagcg tcattcgcga ccccgaggtg cgccaagcga cacatcacct gctcgaccac gtgtgtgaga atcgcctcca gagtgtcttg tccaagatcg gtacaggccc gttcgagacg cagtccatga cccccacaga gctctccacg ctgctagcaa aggatgcgct gaaggacttc ttgaaggagg cggatgcaaa gatcgtcaac acgccgctac tcgtgcgccg cgagatggcc cgctacgttc tttttgaggc gcgcaggtac gtagccagta gatggaagga cgtcgttggg cagcaaaggg aggcagacgg ggagtcgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at