LedoB.10252.a

threonyl-tRNA synthetase, putative

CENTER ID: LedoB.10252.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
LedoB.10252.a.A1.GE44467 full length 1 788
LedoB.10252.a.A2.GE44468 XtalPred domain #1 21 783
LedoB.10252.a.B1.GE42673 full length 1 788
LedoB.10252.a.B2.GE43051 XtalPred domain #1 21 783
LedoB.10252.a.B3.GE43052 XtalPred domain #2 49 783
LedoB.10252.a.B4.GE43713 TEdwards Stma structure domain 285 788
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
LedoB.10252.a.B4.PW38875 TEdwards Stma structure domain 285 788
LedoB.10252.a.A1.PW39085 full length 1 788

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LdBPK_351420.1
UniProt: E9BS18

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGKKKAAEA TVDLATLAEP GFWRQRVEIF EQLWQQQQNR YESMKAPIKV SLPDGKVMDA ESWVTCPLDI AKRLSNSLPD KVIVARVNEA LWDLTRPFEA DCQLELLDWD DKDAREVFWH SSSHVLGYAL ERIFDTKLSV GPALEEGGFF YEGLTNRPVS ESDYKAIESA MQELVKQKMP YQRLEVSKED ALRLFGYTEF KSKILASKVP DGGSCTVYRC GMLIDPCRGP HLPDTGRVKA FAVTKNSSSY FEGKAENEVL QRVYGISFPK NPMLTEWKTI QEEAAKRDHR VIGRQQNLFN FHEVSPGSAF WLPHGTRIYN TLVEFLRKKY RRCGFQEVIS PNMYSSKLWM VSGHWEKYAD NMFCTKCEKE DFGLKPMNCP GHCIMFASQP HSYKELPIRY ADFGVLHRNE LSGALTGLTR VRRFQQDDAH IFCRMDQITD EMEGQMQFLS DVYGVLGFKF YFYHSTRPAN KLGSDALWDQ SESYLRTALN RFCGIPEELP DPLHPDQRFH YDGKPDSVKK MKALMKKAER CEDPNAWKGP TNGGDGWIEN AGDGAFYGPK IDIVVEDALR RRHQCATIQL DFNLPSRFGL KYTLPAAEKD ETTVSPTEKQ KHADPALTAS SPAVEDCVSK PKPGTYEAAV RDLGIDLELD ANQARPVMIH RAILGSLERS IAILCEHFGG DWPFWLSPRQ VMVVPVSASN YEYSQQVRDT MHDAGFHADV DNGAATLDKK IRNAEKARYN FILVVGQKEQ DATAVNIRAR GEKRLGNKSL VEAVQWLKEL ADTHNREY
NT Sequence
atgtctggca agaagaaggc ggcggaggca accgtggacc tggccaccct ggcggagcca ggcttctgga ggcaacgcgt cgaaatcttc gagcagctat ggcagcagca gcagaaccgc tatgagtcga tgaaggcccc gatcaaggtg agcctcccgg atggaaaggt gatggacgcc gagagctggg ttacgtgccc gctggacatc gcaaagcgcc tgtccaactc gctgccagac aaggtcattg tggcccgcgt gaacgaggcg ctgtgggacc tgacgcggcc gttcgaggct gactgtcaac tggagctgct ggactgggac gacaaggatg cgcgtgaggt gttctggcac agctcctcgc acgtgctcgg ctacgcgctc gagcgcatct tcgacacgaa gctctccgtc ggcccggcgc tggaggaggg cggtttcttt tacgagggtc tcaccaatcg cccggtgtcg gagtccgact acaaggcgat cgagtcggcc atgcaggagc tggtgaagca gaagatgcca taccagcgtc tcgaagtgtc gaaggaggac gccctgagac tcttcggcta cacggagttc aagagcaaga tcctggcaag caaggtgccg gacggaggct cgtgcaccgt gtaccggtgc ggtatgctga ttgacccgtg ccgtggtccc cacctcccgg acacgggccg cgtcaaggcc tttgctgtga ccaagaactc ctcctcttac tttgagggca aggccgagaa cgaggtgctg cagcgcgtgt acggcatttc gtttccgaag aaccccatgc tgacggagtg gaagacgatc caggaggagg ccgcgaagcg cgaccaccgc gtaattggcc gccagcagaa cctcttcaac ttccacgagg tctcacctgg cagcgccttc tggctgccgc acggcacccg gatctacaac actctcgtag aattcctgcg caagaagtat cgccgctgcg gctttcagga ggtcatctcg ccgaacatgt actcctccaa actttggatg gtgtcgggcc actgggagaa gtacgccgat aacatgttct gcaccaagtg cgagaaggag gactttggtc tgaagccgat gaactgcccg ggccactgca tcatgttcgc gtcgcagccg cactcgtaca aggagctgcc gattcgctac gccgactttg gcgtgctgca ccggaacgag ctgagcggtg ccttgacggg gctgacccgc gtgcgccgct tccagcagga cgatgcacac atcttctgcc gcatggacca gatcacagat gagatggagg gccagatgca gttcctcagc gacgtttacg gcgtgctggg cttcaagttc tacttttacc actccacccg ccccgcgaac aagctcggct ctgacgcgct ctgggaccag tccgaatcgt atctgcgcac cgcgctcaac cggttctgcg gcattccgga ggagctgccc gatccgttgc acccggacca gcgctttcac tacgacggca agccggactc ggtcaagaag atgaaggcgc tcatgaagaa ggcggagcgc tgcgaggacc cgaacgcgtg gaagggcccg accaacggcg gcgatggctg gatcgagaac gctggggacg gcgccttcta cggccccaag atcgacatcg tcgtcgagga cgcgctgcgc cgccgccacc agtgcgccac gatccagttg gacttcaacc tgccgagccg cttcggcctc aagtacaccc ttcccgccgc agagaaggac gagacgacgg tgagcccaac ggagaagcag aagcatgccg accctgccct gaccgcgtcg tcgccggccg tggaggattg cgtgagcaaa ccgaagcccg gcacgtacga ggctgctgtg cgtgaccttg gcatcgacct cgagctggac gccaaccagg cccgccccgt catgatccac cgcgccattc tcggttcctt ggagcgctcg atcgccattc tgtgcgagca ctttggcggc gactggccct tctggctgag cccccgccaa gtgatggtgg tgcctgtttc ggcctccaac tacgagtatt cacagcaggt gcgcgacacg atgcacgatg ccggcttcca cgcggacgtc gacaacggtg ccgccacgct ggacaagaag atccgcaatg cggaaaaggc gcgctacaac tttatccttg tcgtgggcca gaaggagcag gacgcgaccg ccgtcaacat ccgcgcgcgc ggggagaaga gactgggcaa caagtcactc gtggaggcgg tgcagtggct caaggagctg gcggacacac ataaccggga gtactag
Details for LedoB.10252.a.A1.GE44467
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 92kDa
OBSERVED MW: 92kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGKKKAAE ATVDLATLAE PGFWRQRVEI FEQLWQQQQN RYESMKAPIK VSLPDGKVMD AESWVTCPLD IAKRLSNSLP DKVIVARVNE ALWDLTRPFE ADCQLELLDW DDKDAREVFW HSSSHVLGYA LERIFDTKLS VGPALEEGGF FYEGLTNRPV SESDYKAIES AMQELVKQKM PYQRLEVSKE DALRLFGYTE FKSKILASKV PDGGSCTVYR CGMLIDPCRG PHLPDTGRVK AFAVTKNSSS YFEGKAENEV LQRVYGISFP KNPMLTEWKT IQEEAAKRDH RVIGRQQNLF NFHEVSPGSA FWLPHGTRIY NTLVEFLRKK YRRCGFQEVI SPNMYSSKLW MVSGHWEKYA DNMFCTKCEK EDFGLKPMNC PGHCIMFASQ PHSYKELPIR YADFGVLHRN ELSGALTGLT RVRRFQQDDA HIFCRMDQIT DEMEGQMQFL SDVYGVLGFK FYFYHSTRPA NKLGSDALWD QSESYLRTAL NRFCGIPEEL PDPLHPDQRF HYDGKPDSVK KMKALMKKAE RCEDPNAWKG PTNGGDGWIE NAGDGAFYGP KIDIVVEDAL RRRHQCATIQ LDFNLPSRFG LKYTLPAAEK DETTVSPTEK QKHADPALTA SSPAVEDCVS KPKPGTYEAA VRDLGIDLEL DANQARPVMI HRAILGSLER SIAILCEHFG GDWPFWLSPR QVMVVPVSAS NYEYSQQVRD TMHDAGFHAD VDNGAATLDK KIRNAEKARY NFILVVGQKE QDATAVNIRA RGEKRLGNKS LVEAVQWLKE LADTHNREY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctggcaag aagaaggcgg cggaggcaac cgtggacctg gccaccctgg cggagccagg cttctggagg caacgcgtcg aaatcttcga gcagctatgg cagcagcagc agaaccgcta tgagtcgatg aaggccccga tcaaggtgag cctcccggat ggaaaggtga tggacgccga gagctgggtt acgtgcccgc tggacatcgc aaagcgcctg tccaactcgc tgccagacaa ggtcattgtg gcccgcgtga acgaggcgct gtgggacctg acgcggccgt tcgaggctga ctgtcaactg gagctgctgg actgggacga caaggatgcg cgtgaggtgt tctggcacag ctcctcgcac gtgctcggct acgcgctcga gcgcatcttc gacacgaagc tctccgtcgg cccggcgctg gaggagggcg gtttctttta cgagggtctc accaatcgcc cggtgtcgga gtccgactac aaggcgatcg agtcggccat gcaggagctg gtgaagcaga agatgccata ccagcgtctc gaagtgtcga aggaggacgc cctgagactc ttcggctaca cggagttcaa gagcaagatc ctggcaagca aggtgccgga cggaggctcg tgcaccgtgt accggtgcgg tatgctgatt gacccgtgcc gtggtcccca cctcccggac acgggccgcg tcaaggcctt tgctgtgacc aagaactcct cctcttactt tgagggcaag gccgagaacg aggtgctgca gcgcgtgtac ggcatttcgt ttccgaagaa ccccatgctg acggagtgga agacgatcca ggaggaggcc gcgaagcgcg accaccgcgt aattggccgc cagcagaacc tcttcaactt ccacgaggtc tcacctggca gcgccttctg gctgccgcac ggcacccgga tctacaacac tctcgtagaa ttcctgcgca agaagtatcg ccgctgcggc tttcaggagg tcatctcgcc gaacatgtac tcctccaaac tttggatggt gtcgggccac tgggagaagt acgccgataa catgttctgc accaagtgcg agaaggagga ctttggtctg aagccgatga actgcccggg ccactgcatc atgttcgcgt cgcagccgca ctcgtacaag gagctgccga ttcgctacgc cgactttggc gtgctgcacc ggaacgagct gagcggtgcc ttgacggggc tgacccgcgt gcgccgcttc cagcaggacg atgcacacat cttctgccgc atggaccaga tcacagatga gatggagggc cagatgcagt tcctcagcga cgtttacggc gtgctgggct tcaagttcta cttttaccac tccacccgcc ccgcgaacaa gctcggctct gacgcgctct gggaccagtc cgaatcgtat ctgcgcaccg cgctcaaccg gttctgcggc attccggagg agctgcccga tccgttgcac ccggaccagc gctttcacta cgacggcaag ccggactcgg tcaagaagat gaaggcgctc atgaagaagg cggagcgctg cgaggacccg aacgcgtgga agggcccgac caacggcggc gatggctgga tcgagaacgc tggggacggc gccttctacg gccccaagat cgacatcgtc gtcgaggacg cgctgcgccg ccgccaccag tgcgccacga tccagttgga cttcaacctg ccgagccgct tcggcctcaa gtacaccctt cccgccgcag agaaggacga gacgacggtg agcccaacgg agaagcagaa gcatgccgac cctgccctga ccgcgtcgtc gccggccgtg gaggattgcg tgagcaaacc gaagcccggc acgtacgagg ctgctgtgcg tgaccttggc atcgacctcg agctggacgc caaccaggcc cgccccgtca tgatccaccg cgccattctc ggttccttgg agcgctcgat cgccattctg tgcgagcact ttggcggcga ctggcccttc tggctgagcc cccgccaagt gatggtggtg cctgtttcgg cctccaacta cgagtattca cagcaggtgc gcgacacgat gcacgatgcc ggcttccacg cggacgtcga caacggtgcc gccacgctgg acaagaagat ccgcaatgcg gaaaaggcgc gctacaactt tatccttgtc gtgggccaga aggagcagga cgcgaccgcc gtcaacatcc gcgcgcgcgg ggagaagaga ctgggcaaca agtcactcgt ggaggcggtg cagtggctca aggagctggc ggacacacat aaccgggagt actaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for LedoB.10252.a.A2.GE44468
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 89kDa
OBSERVED MW: 89kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SGFWRQRVEI FEQLWQQQQN RYESMKAPIK VSLPDGKVMD AESWVTCPLD IAKRLSNSLP DKVIVARVNE ALWDLTRPFE ADCQLELLDW DDKDAREVFW HSSSHVLGYA LERIFDTKLS VGPALEEGGF FYEGLTNRPV SESDYKAIES AMQELVKQKM PYQRLEVSKE DALRLFGYTE FKSKILASKV PDGGSCTVYR CGMLIDPCRG PHLPDTGRVK AFAVTKNSSS YFEGKAENEV LQRVYGISFP KNPMLTEWKT IQEEAAKRDH RVIGRQQNLF NFHEVSPGSA FWLPHGTRIY NTLVEFLRKK YRRCGFQEVI SPNMYSSKLW MVSGHWEKYA DNMFCTKCEK EDFGLKPMNC PGHCIMFASQ PHSYKELPIR YADFGVLHRN ELSGALTGLT RVRRFQQDDA HIFCRMDQIT DEMEGQMQFL SDVYGVLGFK FYFYHSTRPA NKLGSDALWD QSESYLRTAL NRFCGIPEEL PDPLHPDQRF HYDGKPDSVK KMKALMKKAE RCEDPNAWKG PTNGGDGWIE NAGDGAFYGP KIDIVVEDAL RRRHQCATIQ LDFNLPSRFG LKYTLPAAEK DETTVSPTEK QKHADPALTA SSPAVEDCVS KPKPGTYEAA VRDLGIDLEL DANQARPVMI HRAILGSLER SIAILCEHFG GDWPFWLSPR QVMVVPVSAS NYEYSQQVRD TMHDAGFHAD VDNGAATLDK KIRNAEKARY NFILVVGQKE QDATAVNIRA RGEKRLGNKS LVEAVQWLKE LADT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcggg cttctggagg caacgcgtcg aaatcttcga gcagctatgg cagcagcagc agaaccgcta tgagtcgatg aaggccccga tcaaggtgag cctcccggat ggaaaggtga tggacgccga gagctgggtt acgtgcccgc tggacatcgc aaagcgcctg tccaactcgc tgccagacaa ggtcattgtg gcccgcgtga acgaggcgct gtgggacctg acgcggccgt tcgaggctga ctgtcaactg gagctgctgg actgggacga caaggatgcg cgtgaggtgt tctggcacag ctcctcgcac gtgctcggct acgcgctcga gcgcatcttc gacacgaagc tctccgtcgg cccggcgctg gaggagggcg gtttctttta cgagggtctc accaatcgcc cggtgtcgga gtccgactac aaggcgatcg agtcggccat gcaggagctg gtgaagcaga agatgccata ccagcgtctc gaagtgtcga aggaggacgc cctgagactc ttcggctaca cggagttcaa gagcaagatc ctggcaagca aggtgccgga cggaggctcg tgcaccgtgt accggtgcgg tatgctgatt gacccgtgcc gtggtcccca cctcccggac acgggccgcg tcaaggcctt tgctgtgacc aagaactcct cctcttactt tgagggcaag gccgagaacg aggtgctgca gcgcgtgtac ggcatttcgt ttccgaagaa ccccatgctg acggagtgga agacgatcca ggaggaggcc gcgaagcgcg accaccgcgt aattggccgc cagcagaacc tcttcaactt ccacgaggtc tcacctggca gcgccttctg gctgccgcac ggcacccgga tctacaacac tctcgtagaa ttcctgcgca agaagtatcg ccgctgcggc tttcaggagg tcatctcgcc gaacatgtac tcctccaaac tttggatggt gtcgggccac tgggagaagt acgccgataa catgttctgc accaagtgcg agaaggagga ctttggtctg aagccgatga actgcccggg ccactgcatc atgttcgcgt cgcagccgca ctcgtacaag gagctgccga ttcgctacgc cgactttggc gtgctgcacc ggaacgagct gagcggtgcc ttgacggggc tgacccgcgt gcgccgcttc cagcaggacg atgcacacat cttctgccgc atggaccaga tcacagatga gatggagggc cagatgcagt tcctcagcga cgtttacggc gtgctgggct tcaagttcta cttttaccac tccacccgcc ccgcgaacaa gctcggctct gacgcgctct gggaccagtc cgaatcgtat ctgcgcaccg cgctcaaccg gttctgcggc attccggagg agctgcccga tccgttgcac ccggaccagc gctttcacta cgacggcaag ccggactcgg tcaagaagat gaaggcgctc atgaagaagg cggagcgctg cgaggacccg aacgcgtgga agggcccgac caacggcggc gatggctgga tcgagaacgc tggggacggc gccttctacg gccccaagat cgacatcgtc gtcgaggacg cgctgcgccg ccgccaccag tgcgccacga tccagttgga cttcaacctg ccgagccgct tcggcctcaa gtacaccctt cccgccgcag agaaggacga gacgacggtg agcccaacgg agaagcagaa gcatgccgac cctgccctga ccgcgtcgtc gccggccgtg gaggattgcg tgagcaaacc gaagcccggc acgtacgagg ctgctgtgcg tgaccttggc atcgacctcg agctggacgc caaccaggcc cgccccgtca tgatccaccg cgccattctc ggttccttgg agcgctcgat cgccattctg tgcgagcact ttggcggcga ctggcccttc tggctgagcc cccgccaagt gatggtggtg cctgtttcgg cctccaacta cgagtattca cagcaggtgc gcgacacgat gcacgatgcc ggcttccacg cggacgtcga caacggtgcc gccacgctgg acaagaagat ccgcaatgcg gaaaaggcgc gctacaactt tatccttgtc gtgggccaga aggagcagga cgcgaccgcc gtcaacatcc gcgcgcgcgg ggagaagaga ctgggcaaca agtcactcgt ggaggcggtg cagtggctca aggagctggc ggacacataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for LedoB.10252.a.B1.GE42673
HARVESTED ON: 9/28/2018
SEQUENCED ON: 10/17/2018
EXPECTED MW: 91kDa
OBSERVED MW: 92kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS GKKKAAEATV DLATLAEPGF WRQRVEIFEQ LWQQQQNRYE SMKAPIKVSL PDGKVMDAES WVTCPLDIAK RLSNSLPDKV IVARVNEALW DLTRPFEADC QLELLDWDDK DAREVFWHSS SHVLGYALER IFDTKLSVGP ALEEGGFFYE GLTNRPVSES DYKAIESAMQ ELVKQKMPYQ RLEVSKEDAL RLFGYTEFKS KILASKVPDG GSCTVYRCGM LIDPCRGPHL PDTGRVKAFA VTKNSSSYFE GKAENEVLQR VYGISFPKNP MLTEWKTIQE EAAKRDHRVI GRQQNLFNFH EVSPGSAFWL PHGTRIYNTL VEFLRKKYRR CGFQEVISPN MYSSKLWMVS GHWEKYADNM FCTKCEKEDF GLKPMNCPGH CIMFASQPHS YKELPIRYAD FGVLHRNELS GALTGLTRVR RFQQDDAHIF CRMDQITDEM EGQMQFLSDV YGVLGFKFYF YHSTRPANKL GSDALWDQSE SYLRTALNRF CGIPEELPDP LHPDQRFHYD GKPDSVKKMK ALMKKAERCE DPNAWKGPTN GGDGWIENAG DGAFYGPKID IVVEDALRRR HQCATIQLDF NLPSRFGLKY TLPAAEKDET TVSPTEKQKH ADPALTASSP AVEDCVSKPK PGTYEAAVRD LGIDLELDAN QARPVMIHRA ILGSLERSIA ILCEHFGGDW PFWLSPRQVM VVPVSASNYE YSQQVRDTMH DAGFHADVDN GAATLDKKIR NAEKARYNFI LVVGQKEQDA TAVNIRARGE KRLGNKSLVE AVQWLKELAD THNREY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg gcaagaagaa ggcggcggag gcaaccgtgg acctggccac cctggcggag ccaggcttct ggaggcaacg cgtcgaaatc ttcgagcagc tatggcagca gcagcagaac cgctatgagt cgatgaaggc cccgatcaag gtgagcctcc cggatggaaa ggtgatggac gccgagagct gggttacgtg cccgctggac atcgcaaagc gcctgtccaa ctcgctgcca gacaaggtca ttgtggcccg cgtgaacgag gcgctgtggg acctgacgcg gccgttcgag gctgactgtc aactggagct gctggactgg gacgacaagg atgcgcgtga ggtgttctgg cacagctcct cgcacgtgct cggctacgcg ctcgagcgca tcttcgacac gaagctctcc gtcggcccgg cgctggagga gggcggtttc ttttacgagg gtctcaccaa tcgcccggtg tcggagtccg actacaaggc gatcgagtcg gccatgcagg agctggtgaa gcagaagatg ccataccagc gtctcgaagt gtcgaaggag gacgccctga gactcttcgg ctacacggag ttcaagagca agatcctggc aagcaaggtg ccggacggag gctcgtgcac cgtgtaccgg tgcggtatgc tgattgaccc gtgccgtggt ccccacctcc cggacacggg ccgcgtcaag gcctttgctg tgaccaagaa ctcctcctct tactttgagg gcaaggccga gaacgaggtg ctgcagcgcg tgtacggcat ttcgtttccg aagaacccca tgctgacgga gtggaagacg atccaggagg aggccgcgaa gcgcgaccac cgcgtaattg gccgccagca gaacctcttc aacttccacg aggtctcacc tggcagcgcc ttctggctgc cgcacggcac ccggatctac aacactctcg tagaattcct gcgcaagaag tatcgccgct gcggctttca ggaggtcatc tcgccgaaca tgtactcctc caaactttgg atggtgtcgg gccactggga gaagtacgcc gataacatgt tctgcaccaa gtgcgagaag gaggactttg gtctgaagcc gatgaactgc ccgggccact gcatcatgtt cgcgtcgcag ccgcactcgt acaaggagct gccgattcgc tacgccgact ttggcgtgct gcaccggaac gagctgagcg gtgccttgac ggggctgacc cgcgtgcgcc gcttccagca ggacgatgca cacatcttct gccgcatgga ccagatcaca gatgagatgg agggccagat gcagttcctc agcgacgttt acggcgtgct gggcttcaag ttctactttt accactccac ccgccccgcg aacaagctcg gctctgacgc gctctgggac cagtccgaat cgtatctgcg caccgcgctc aaccggttct gcggcattcc ggaggagctg cccgatccgt tgcacccgga ccagcgcttt cactacgacg gcaagccgga ctcggtcaag aagatgaagg cgctcatgaa gaaggcggag cgctgcgagg acccgaacgc gtggaagggc ccgaccaacg gcggcgatgg ctggatcgag aacgctgggg acggcgcctt ctacggcccc aagatcgaca tcgtcgtcga ggacgcgctg cgccgccgcc accagtgcgc cacgatccag ttggacttca acctgccgag ccgcttcggc ctcaagtaca cccttcccgc cgcagagaag gacgagacga cggtgagccc aacggagaag cagaagcatg ccgaccctgc cctgaccgcg tcgtcgccgg ccgtggagga ttgcgtgagc aaaccgaagc ccggcacgta cgaggctgct gtgcgtgacc ttggcatcga cctcgagctg gacgccaacc aggcccgccc cgtcatgatc caccgcgcca ttctcggttc cttggagcgc tcgatcgcca ttctgtgcga gcactttggc ggcgactggc ccttctggct gagcccccgc caagtgatgg tggtgcctgt ttcggcctcc aactacgagt attcacagca ggtgcgcgac acgatgcacg atgccggctt ccacgcggac gtcgacaacg gtgccgccac gctggacaag aagatccgca atgcggaaaa ggcgcgctac aactttatcc ttgtcgtggg ccagaaggag caggacgcga ccgccgtcaa catccgcgcg cgcggggaga agagactggg caacaagtca ctcgtggagg cggtgcagtg gctcaaggag ctggcggaca cacataaccg ggagtactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for LedoB.10252.a.B2.GE43051
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/25/2019
EXPECTED MW: 88kDa
OBSERVED MW: 88kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHGF WRQRVEIFEQ LWQQQQNRYE SMKAPIKVSL PDGKVMDAES WVTCPLDIAK RLSNSLPDKV IVARVNEALW DLTRPFEADC QLELLDWDDK DAREVFWHSS SHVLGYALER IFDTKLSVGP ALEEGGFFYE GLTNRPVSES DYKAIESAMQ ELVKQKMPYQ RLEVSKEDAL RLFGYTEFKS KILASKVPDG GSCTVYRCGM LIDPCRGPHL PDTGRVKAFA VTKNSSSYFE GKAENEVLQR VYGISFPKNP MLTEWKTIQE EAAKRDHRVI GRQQNLFNFH EVSPGSAFWL PHGTRIYNTL VEFLRKKYRR CGFQEVISPN MYSSKLWMVS GHWEKYADNM FCTKCEKEDF GLKPMNCPGH CIMFASQPHS YKELPIRYAD FGVLHRNELS GALTGLTRVR RFQQDDAHIF CRMDQITDEM EGQMQFLSDV YGVLGFKFYF YHSTRPANKL GSDALWDQSE SYLRTALNRF CGIPEELPDP LHPDQRFHYD GKPDSVKKMK ALMKKAERCE DPNAWKGPTN GGDGWIENAG DGAFYGPKID IVVEDALRRR HQCATIQLDF NLPSRFGLKY TLPAAEKDET TVSPTEKQKH ADPALTASSP AVEDCVSKPK PGTYEAAVRD LGIDLELDAN QARPVMIHRA ILGSLERSIA ILCEHFGGDW PFWLSPRQVM VVPVSASNYE YSQQVRDTMH DAGFHADVDN GAATLDKKIR NAEKARYNFI LVVGQKEQDA TAVNIRARGE KRLGNKSLVE AVQWLKELAD T
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggcttct ggaggcaacg cgtcgaaatc ttcgagcagc tatggcagca gcagcagaac cgctatgagt cgatgaaggc cccgatcaag gtgagcctcc cggatggaaa ggtgatggac gccgagagct gggttacgtg cccgctggac atcgcaaagc gcctgtccaa ctcgctgcca gacaaggtca ttgtggcccg cgtgaacgag gcgctgtggg acctgacgcg gccgttcgag gctgactgtc aactggagct gctggactgg gacgacaagg atgcgcgtga ggtgttctgg cacagctcct cgcacgtgct cggctacgcg ctcgagcgca tcttcgacac gaagctctcc gtcggcccgg cgctggagga gggcggtttc ttttacgagg gtctcaccaa tcgcccggtg tcggagtccg actacaaggc gatcgagtcg gccatgcagg agctggtgaa gcagaagatg ccataccagc gtctcgaagt gtcgaaggag gacgccctga gactcttcgg ctacacggag ttcaagagca agatcctggc aagcaaggtg ccggacggag gctcgtgcac cgtgtaccgg tgcggtatgc tgattgaccc gtgccgtggt ccccacctcc cggacacggg ccgcgtcaag gcctttgctg tgaccaagaa ctcctcctct tactttgagg gcaaggccga gaacgaggtg ctgcagcgcg tgtacggcat ttcgtttccg aagaacccca tgctgacgga gtggaagacg atccaggagg aggccgcgaa gcgcgaccac cgcgtaattg gccgccagca gaacctcttc aacttccacg aggtctcacc tggcagcgcc ttctggctgc cgcacggcac ccggatctac aacactctcg tagaattcct gcgcaagaag tatcgccgct gcggctttca ggaggtcatc tcgccgaaca tgtactcctc caaactttgg atggtgtcgg gccactggga gaagtacgcc gataacatgt tctgcaccaa gtgcgagaag gaggactttg gtctgaagcc gatgaactgc ccgggccact gcatcatgtt cgcgtcgcag ccgcactcgt acaaggagct gccgattcgc tacgccgact ttggcgtgct gcaccggaac gagctgagcg gtgccttgac ggggctgacc cgcgtgcgcc gcttccagca ggacgatgca cacatcttct gccgcatgga ccagatcaca gatgagatgg agggccagat gcagttcctc agcgacgttt acggcgtgct gggcttcaag ttctactttt accactccac ccgccccgcg aacaagctcg gctctgacgc gctctgggac cagtccgaat cgtatctgcg caccgcgctc aaccggttct gcggcattcc ggaggagctg cccgatccgt tgcacccgga ccagcgcttt cactacgacg gcaagccgga ctcggtcaag aagatgaagg cgctcatgaa gaaggcggag cgctgcgagg acccgaacgc gtggaagggc ccgaccaacg gcggcgatgg ctggatcgag aacgctgggg acggcgcctt ctacggcccc aagatcgaca tcgtcgtcga ggacgcgctg cgccgccgcc accagtgcgc cacgatccag ttggacttca acctgccgag ccgcttcggc ctcaagtaca cccttcccgc cgcagagaag gacgagacga cggtgagccc aacggagaag cagaagcatg ccgaccctgc cctgaccgcg tcgtcgccgg ccgtggagga ttgcgtgagc aaaccgaagc ccggcacgta cgaggctgct gtgcgtgacc ttggcatcga cctcgagctg gacgccaacc aggcccgccc cgtcatgatc caccgcgcca ttctcggttc cttggagcgc tcgatcgcca ttctgtgcga gcactttggc ggcgactggc ccttctggct gagcccccgc caagtgatgg tggtgcctgt ttcggcctcc aactacgagt attcacagca ggtgcgcgac acgatgcacg atgccggctt ccacgcggac gtcgacaacg gtgccgccac gctggacaag aagatccgca atgcggaaaa ggcgcgctac aactttatcc ttgtcgtggg ccagaaggag caggacgcga ccgccgtcaa catccgcgcg cgcggggaga agagactggg caacaagtca ctcgtggagg cggtgcagtg gctcaaggag ctggcggaca catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for LedoB.10252.a.B3.GE43052
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/25/2019
EXPECTED MW: 84kDa
OBSERVED MW: 84kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 16
Validated AA Sequence
MIHRAILGSL ERSIAILCEH FGGDWPFWLS PRQVMVVPVS ASNYEYSQQV RDTMHDAGFH ADVDNGAATL DKKIRNAEKA RYNFILVVGQ KEQDATAVNI RARGEKRLGN KSLVEAVXXX XELADT
Validated NT Sequence
atcttactca cttatgtgtc cgccagctcn nnnnnnnnnn nnaccgcctc cacgagtgac ttgttgccca gtctcttctc cccgcgcgcg cggatgttga cggcggtcgc gtcctgctcc ttctggccca cgacaaggat aaagttgtag cgcgcctttt ccgcattgcg gatcttcttg tccagcgtgg cggcaccgtt gtcgacgtcc gcgtggaagc cggcatcgtg catcgtgtcg cgcacctgct gtgaatactc gtagttggag gccgaaacag gcaccaccat cacttggcgg gggctcagcc agaagggcca gtcgccgcca aagtgctcgc acagaatggc gatcgagcgc tccaaggaac cgagaatggc gcggtggatc atgacggggc gggcctggtt ggcgtccagc tcgaggtcga tgccaaggtc acgcacagca gcctcgtacg tgccgggctt cggtttgctc acgcaatcct ccacggccgg cgacgacgcg gtcagggcag ggtcggcatg cttctgcttc tccgttgggc tcaccgtcgt ctcgtccttc tctgcggcgg gaagggtgta cttgaggccg aagcggctcg gcaggttgaa gtccaactgg atcgtggcgc actggtggcg gcggcgcagc gcgtcctcga cgacgatgtc gatcttgggg ccgtagaagg cgccgtcccc agcgttctcg atccnnncat cgccgcnnnt ggtcgggccc ttccacgcgt tcnggnncct cgcagcgctc cgccttcttc atgagcgncn nnnnntttct tgaccnnnnn nnnnnnnnnn ncgnnnngaa nnnnntgnnn nnnnnncnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnt
Expected Protein Sequence
MAHHHHHHKV SLPDGKVMDA ESWVTCPLDI AKRLSNSLPD KVIVARVNEA LWDLTRPFEA DCQLELLDWD DKDAREVFWH SSSHVLGYAL ERIFDTKLSV GPALEEGGFF YEGLTNRPVS ESDYKAIESA MQELVKQKMP YQRLEVSKED ALRLFGYTEF KSKILASKVP DGGSCTVYRC GMLIDPCRGP HLPDTGRVKA FAVTKNSSSY FEGKAENEVL QRVYGISFPK NPMLTEWKTI QEEAAKRDHR VIGRQQNLFN FHEVSPGSAF WLPHGTRIYN TLVEFLRKKY RRCGFQEVIS PNMYSSKLWM VSGHWEKYAD NMFCTKCEKE DFGLKPMNCP GHCIMFASQP HSYKELPIRY ADFGVLHRNE LSGALTGLTR VRRFQQDDAH IFCRMDQITD EMEGQMQFLS DVYGVLGFKF YFYHSTRPAN KLGSDALWDQ SESYLRTALN RFCGIPEELP DPLHPDQRFH YDGKPDSVKK MKALMKKAER CEDPNAWKGP TNGGDGWIEN AGDGAFYGPK IDIVVEDALR RRHQCATIQL DFNLPSRFGL KYTLPAAEKD ETTVSPTEKQ KHADPALTAS SPAVEDCVSK PKPGTYEAAV RDLGIDLELD ANQARPVMIH RAILGSLERS IAILCEHFGG DWPFWLSPRQ VMVVPVSASN YEYSQQVRDT MHDAGFHADV DNGAATLDKK IRNAEKARYN FILVVGQKEQ DATAVNIRAR GEKRLGNKSL VEAVQWLKEL ADT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaggtga gcctcccgga tggaaaggtg atggacgccg agagctgggt tacgtgcccg ctggacatcg caaagcgcct gtccaactcg ctgccagaca aggtcattgt ggcccgcgtg aacgaggcgc tgtgggacct gacgcggccg ttcgaggctg actgtcaact ggagctgctg gactgggacg acaaggatgc gcgtgaggtg ttctggcaca gctcctcgca cgtgctcggc tacgcgctcg agcgcatctt cgacacgaag ctctccgtcg gcccggcgct ggaggagggc ggtttctttt acgagggtct caccaatcgc ccggtgtcgg agtccgacta caaggcgatc gagtcggcca tgcaggagct ggtgaagcag aagatgccat accagcgtct cgaagtgtcg aaggaggacg ccctgagact cttcggctac acggagttca agagcaagat cctggcaagc aaggtgccgg acggaggctc gtgcaccgtg taccggtgcg gtatgctgat tgacccgtgc cgtggtcccc acctcccgga cacgggccgc gtcaaggcct ttgctgtgac caagaactcc tcctcttact ttgagggcaa ggccgagaac gaggtgctgc agcgcgtgta cggcatttcg tttccgaaga accccatgct gacggagtgg aagacgatcc aggaggaggc cgcgaagcgc gaccaccgcg taattggccg ccagcagaac ctcttcaact tccacgaggt ctcacctggc agcgccttct ggctgccgca cggcacccgg atctacaaca ctctcgtaga attcctgcgc aagaagtatc gccgctgcgg ctttcaggag gtcatctcgc cgaacatgta ctcctccaaa ctttggatgg tgtcgggcca ctgggagaag tacgccgata acatgttctg caccaagtgc gagaaggagg actttggtct gaagccgatg aactgcccgg gccactgcat catgttcgcg tcgcagccgc actcgtacaa ggagctgccg attcgctacg ccgactttgg cgtgctgcac cggaacgagc tgagcggtgc cttgacgggg ctgacccgcg tgcgccgctt ccagcaggac gatgcacaca tcttctgccg catggaccag atcacagatg agatggaggg ccagatgcag ttcctcagcg acgtttacgg cgtgctgggc ttcaagttct acttttacca ctccacccgc cccgcgaaca agctcggctc tgacgcgctc tgggaccagt ccgaatcgta tctgcgcacc gcgctcaacc ggttctgcgg cattccggag gagctgcccg atccgttgca cccggaccag cgctttcact acgacggcaa gccggactcg gtcaagaaga tgaaggcgct catgaagaag gcggagcgct gcgaggaccc gaacgcgtgg aagggcccga ccaacggcgg cgatggctgg atcgagaacg ctggggacgg cgccttctac ggccccaaga tcgacatcgt cgtcgaggac gcgctgcgcc gccgccacca gtgcgccacg atccagttgg acttcaacct gccgagccgc ttcggcctca agtacaccct tcccgccgca gagaaggacg agacgacggt gagcccaacg gagaagcaga agcatgccga ccctgccctg accgcgtcgt cgccggccgt ggaggattgc gtgagcaaac cgaagcccgg cacgtacgag gctgctgtgc gtgaccttgg catcgacctc gagctggacg ccaaccaggc ccgccccgtc atgatccacc gcgccattct cggttccttg gagcgctcga tcgccattct gtgcgagcac tttggcggcg actggccctt ctggctgagc ccccgccaag tgatggtggt gcctgtttcg gcctccaact acgagtattc acagcaggtg cgcgacacga tgcacgatgc cggcttccac gcggacgtcg acaacggtgc cgccacgctg gacaagaaga tccgcaatgc ggaaaaggcg cgctacaact ttatccttgt cgtgggccag aaggagcagg acgcgaccgc cgtcaacatc cgcgcgcgcg gggagaagag actgggcaac aagtcactcg tggaggcggt gcagtggctc aaggagctgg cggacacatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for LedoB.10252.a.B4.GE43713
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 52
Validated AA Sequence
MKALMKKAER CEDPNAWKGP TNGGDGWIEN AGDGAFYGPK IDIVVEDALR RRHQCATIQL DFNLPSRFGL KYTLPAAEKD ETTVSPTEKQ KHADPALTAS SPAVEDCVSK PKPGTYEAAV RDLGIDLELD ANQARPVMIH RAILGSLERS IAILCEHFGG DWPFWLSPRQ VMVVPVSASN YEYSQQVRDT MHDAGFHADV DNGAATLDKK IRNAEKARYN FILVVGQKEQ DATAVNIRAR GEKRLGNKSL VEAVQWLKEL XDXHNREY
Validated NT Sequence
ggctnnngtt agcagccgga tcctatctta ctcacttagt actcccggtt atgtgngtcn nnnagctcct tgagccactg caccgcctcc acgagtgact tgttgcccag tctcttctcc ccgcgcgcgc ggatgttgac ggcggtcgcg tcctgctcct tctggcccac gacaaggata aagttgtagc gcgccttttc cgcattgcgg atcttcttgt ccagcgtggc ggcaccgttg tcgacgtccg cgtggaagcc ggcatcgtgc atcgtgtcgc gcacctgctg tgaatactcg tagttggagg ccgaaacagg caccaccatc acttggcggg ggctcagcca gaagggccag tcgccgccaa agtgctcgca cagaatggcg atcgagcgct ccaaggaacc gagaatggcg cggtggatca tgacggggcg ggcctggttg gcgtccagct cgaggtcgat gccaaggtca cgcacagcag cctcgtacgt gccgggcttc ggtttgctca cgcaatcctc cacggccggc gacgacgcgg tcagggcagg gtcggcatgc ttctgcttct ccgttgggct caccgtcgtc tcgtccttct ctgcggcggg aagggtgtac ttgaggccga agcggctcgg caggttgaag tccaactgga tcgtggcgca ctggtggcgg cggcgcagcg cgtcctcgac gacgatgtcg atcttggggc cgtagaaggc gccgtcccca gcgttctcga tccanccatc gccgccgttg gtcgggccct tccacgcgtt cgggtcctcg cagcgctccg ccttcttcat gagcgccttc atcttcttga ccgantccgg cttgccgtcg tantgaaagc gctggtccng ntnnnacgga tncgggcagn nncntccgga atgncgcagn anccggntga gcgcggtgcg cnnatacgat tcngactggt cncanagcnc gtcananccg annttgttcg cnnnnnnnnn tgnnaaaagt agaacttgaa
Expected Protein Sequence
MAHHHHHHAK RDHRVIGRQQ NLFNFHEVSP GSAFWLPHGT RIYNTLVEFL RKKYRRCGFQ EVISPNMYSS KLWMVSGHWE KYADNMFCTK CEKEDFGLKP MNCPGHCIMF ASQPHSYKEL PIRYADFGVL HRNELSGALT GLTRVRRFQQ DDAHIFCRMD QITDEMEGQM QFLSDVYGVL GFKFYFYHST RPANKLGSDA LWDQSESYLR TALNRFCGIP EELPDPLHPD QRFHYDGKPD SVKKMKALMK KAERCEDPNA WKGPTNGGDG WIENAGDGAF YGPKIDIVVE DALRRRHQCA TIQLDFNLPS RFGLKYTLPA AEKDETTVSP TEKQKHADPA LTASSPAVED CVSKPKPGTY EAAVRDLGID LELDANQARP VMIHRAILGS LERSIAILCE HFGGDWPFWL SPRQVMVVPV SASNYEYSQQ VRDTMHDAGF HADVDNGAAT LDKKIRNAEK ARYNFILVVG QKEQDATAVN IRARGEKRLG NKSLVEAVQW LKELADTHNR EY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgaagc gcgaccaccg cgtaattggc cgccagcaga acctcttcaa cttccacgag gtctcacctg gcagcgcctt ctggctgccg cacggcaccc ggatctacaa cactctcgta gaattcctgc gcaagaagta tcgccgctgc ggctttcagg aggtcatctc gccgaacatg tactcctcca aactttggat ggtgtcgggc cactgggaga agtacgccga taacatgttc tgcaccaagt gcgagaagga ggactttggt ctgaagccga tgaactgccc gggccactgc atcatgttcg cgtcgcagcc gcactcgtac aaggagctgc cgattcgcta cgccgacttt ggcgtgctgc accggaacga gctgagcggt gccttgacgg ggctgacccg cgtgcgccgc ttccagcagg acgatgcaca catcttctgc cgcatggacc agatcacaga tgagatggag ggccagatgc agttcctcag cgacgtttac ggcgtgctgg gcttcaagtt ctacttttac cactccaccc gccccgcgaa caagctcggc tctgacgcgc tctgggacca gtccgaatcg tatctgcgca ccgcgctcaa ccggttctgc ggcattccgg aggagctgcc cgatccgttg cacccggacc agcgctttca ctacgacggc aagccggact cggtcaagaa gatgaaggcg ctcatgaaga aggcggagcg ctgcgaggac ccgaacgcgt ggaagggccc gaccaacggc ggcgatggct ggatcgagaa cgctggggac ggcgccttct acggccccaa gatcgacatc gtcgtcgagg acgcgctgcg ccgccgccac cagtgcgcca cgatccagtt ggacttcaac ctgccgagcc gcttcggcct caagtacacc cttcccgccg cagagaagga cgagacgacg gtgagcccaa cggagaagca gaagcatgcc gaccctgccc tgaccgcgtc gtcgccggcc gtggaggatt gcgtgagcaa accgaagccc ggcacgtacg aggctgctgt gcgtgacctt ggcatcgacc tcgagctgga cgccaaccag gcccgccccg tcatgatcca ccgcgccatt ctcggttcct tggagcgctc gatcgccatt ctgtgcgagc actttggcgg cgactggccc ttctggctga gcccccgcca agtgatggtg gtgcctgttt cggcctccaa ctacgagtat tcacagcagg tgcgcgacac gatgcacgat gccggcttcc acgcggacgt cgacaacggt gccgccacgc tggacaagaa gatccgcaat gcggaaaagg cgcgctacaa ctttatcctt gtcgtgggcc agaaggagca ggacgcgacc gccgtcaaca tccgcgcgcg cggggagaag agactgggca acaagtcact cgtggaggcg gtgcagtggc tcaaggagct ggcggacaca cataaccggg agtactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for LedoB.10252.a.B4.PW38875
PURIFICATION DATe: 8/11/2020
CONCENTRATION: 11mg/ml
OBSERVED MW: 58kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 52
Protocol Notes
notes unavailable
Validated AA Sequence
MKALMKKAER CEDPNAWKGP TNGGDGWIEN AGDGAFYGPK IDIVVEDALR RRHQCATIQL DFNLPSRFGL KYTLPAAEKD ETTVSPTEKQ KHADPALTAS SPAVEDCVSK PKPGTYEAAV RDLGIDLELD ANQARPVMIH RAILGSLERS IAILCEHFGG DWPFWLSPRQ VMVVPVSASN YEYSQQVRDT MHDAGFHADV DNGAATLDKK IRNAEKARYN FILVVGQKEQ DATAVNIRAR GEKRLGNKSL VEAVQWLKEL XDXHNREY
Validated NT Sequence
ggctnnngtt agcagccgga tcctatctta ctcacttagt actcccggtt atgtgngtcn nnnagctcct tgagccactg caccgcctcc acgagtgact tgttgcccag tctcttctcc ccgcgcgcgc ggatgttgac ggcggtcgcg tcctgctcct tctggcccac gacaaggata aagttgtagc gcgccttttc cgcattgcgg atcttcttgt ccagcgtggc ggcaccgttg tcgacgtccg cgtggaagcc ggcatcgtgc atcgtgtcgc gcacctgctg tgaatactcg tagttggagg ccgaaacagg caccaccatc acttggcggg ggctcagcca gaagggccag tcgccgccaa agtgctcgca cagaatggcg atcgagcgct ccaaggaacc gagaatggcg cggtggatca tgacggggcg ggcctggttg gcgtccagct cgaggtcgat gccaaggtca cgcacagcag cctcgtacgt gccgggcttc ggtttgctca cgcaatcctc cacggccggc gacgacgcgg tcagggcagg gtcggcatgc ttctgcttct ccgttgggct caccgtcgtc tcgtccttct ctgcggcggg aagggtgtac ttgaggccga agcggctcgg caggttgaag tccaactgga tcgtggcgca ctggtggcgg cggcgcagcg cgtcctcgac gacgatgtcg atcttggggc cgtagaaggc gccgtcccca gcgttctcga tccanccatc gccgccgttg gtcgggccct tccacgcgtt cgggtcctcg cagcgctccg ccttcttcat gagcgccttc atcttcttga ccgantccgg cttgccgtcg tantgaaagc gctggtccng ntnnnacgga tncgggcagn nncntccgga atgncgcagn anccggntga gcgcggtgcg cnnatacgat tcngactggt cncanagcnc gtcananccg annttgttcg cnnnnnnnnn tgnnaaaagt agaacttgaa
Expressed Protein Sequence
MAHHHHHHAK RDHRVIGRQQ NLFNFHEVSP GSAFWLPHGT RIYNTLVEFL RKKYRRCGFQ EVISPNMYSS KLWMVSGHWE KYADNMFCTK CEKEDFGLKP MNCPGHCIMF ASQPHSYKEL PIRYADFGVL HRNELSGALT GLTRVRRFQQ DDAHIFCRMD QITDEMEGQM QFLSDVYGVL GFKFYFYHST RPANKLGSDA LWDQSESYLR TALNRFCGIP EELPDPLHPD QRFHYDGKPD SVKKMKALMK KAERCEDPNA WKGPTNGGDG WIENAGDGAF YGPKIDIVVE DALRRRHQCA TIQLDFNLPS RFGLKYTLPA AEKDETTVSP TEKQKHADPA LTASSPAVED CVSKPKPGTY EAAVRDLGID LELDANQARP VMIHRAILGS LERSIAILCE HFGGDWPFWL SPRQVMVVPV SASNYEYSQQ VRDTMHDAGF HADVDNGAAT LDKKIRNAEK ARYNFILVVG QKEQDATAVN IRARGEKRLG NKSLVEAVQW LKELADTHNR EY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCGAAGC GCGACCACCG CGTAATTGGC CGCCAGCAGA ACCTCTTCAA CTTCCACGAG GTCTCACCTG GCAGCGCCTT CTGGCTGCCG CACGGCACCC GGATCTACAA CACTCTCGTA GAATTCCTGC GCAAGAAGTA TCGCCGCTGC GGCTTTCAGG AGGTCATCTC GCCGAACATG TACTCCTCCA AACTTTGGAT GGTGTCGGGC CACTGGGAGA AGTACGCCGA TAACATGTTC TGCACCAAGT GCGAGAAGGA GGACTTTGGT CTGAAGCCGA TGAACTGCCC GGGCCACTGC ATCATGTTCG CGTCGCAGCC GCACTCGTAC AAGGAGCTGC CGATTCGCTA CGCCGACTTT GGCGTGCTGC ACCGGAACGA GCTGAGCGGT GCCTTGACGG GGCTGACCCG CGTGCGCCGC TTCCAGCAGG ACGATGCACA CATCTTCTGC CGCATGGACC AGATCACAGA TGAGATGGAG GGCCAGATGC AGTTCCTCAG CGACGTTTAC GGCGTGCTGG GCTTCAAGTT CTACTTTTAC CACTCCACCC GCCCCGCGAA CAAGCTCGGC TCTGACGCGC TCTGGGACCA GTCCGAATCG TATCTGCGCA CCGCGCTCAA CCGGTTCTGC GGCATTCCGG AGGAGCTGCC CGATCCGTTG CACCCGGACC AGCGCTTTCA CTACGACGGC AAGCCGGACT CGGTCAAGAA GATGAAGGCG CTCATGAAGA AGGCGGAGCG CTGCGAGGAC CCGAACGCGT GGAAGGGCCC GACCAACGGC GGCGATGGCT GGATCGAGAA CGCTGGGGAC GGCGCCTTCT ACGGCCCCAA GATCGACATC GTCGTCGAGG ACGCGCTGCG CCGCCGCCAC CAGTGCGCCA CGATCCAGTT GGACTTCAAC CTGCCGAGCC GCTTCGGCCT CAAGTACACC CTTCCCGCCG CAGAGAAGGA CGAGACGACG GTGAGCCCAA CGGAGAAGCA GAAGCATGCC GACCCTGCCC TGACCGCGTC GTCGCCGGCC GTGGAGGATT GCGTGAGCAA ACCGAAGCCC GGCACGTACG AGGCTGCTGT GCGTGACCTT GGCATCGACC TCGAGCTGGA CGCCAACCAG GCCCGCCCCG TCATGATCCA CCGCGCCATT CTCGGTTCCT TGGAGCGCTC GATCGCCATT CTGTGCGAGC ACTTTGGCGG CGACTGGCCC TTCTGGCTGA GCCCCCGCCA AGTGATGGTG GTGCCTGTTT CGGCCTCCAA CTACGAGTAT TCACAGCAGG TGCGCGACAC GATGCACGAT GCCGGCTTCC ACGCGGACGT CGACAACGGT GCCGCCACGC TGGACAAGAA GATCCGCAAT GCGGAAAAGG CGCGCTACAA CTTTATCCTT GTCGTGGGCC AGAAGGAGCA GGACGCGACC GCCGTCAACA TCCGCGCGCG CGGGGAGAAG AGACTGGGCA ACAAGTCACT CGTGGAGGCG GTGCAGTGGC TCAAGGAGCT GGCGGACACA CATAACCGGG AGTACTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT
Details for LedoB.10252.a.A1.PW39085
PURIFICATION DATe: 9/9/2021
CONCENTRATION: 35mg/ml
OBSERVED MW: 92kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGKKKAAE ATVDLATLAE PGFWRQRVEI FEQLWQQQQN RYESMKAPIK VSLPDGKVMD AESWVTCPLD IAKRLSNSLP DKVIVARVNE ALWDLTRPFE ADCQLELLDW DDKDAREVFW HSSSHVLGYA LERIFDTKLS VGPALEEGGF FYEGLTNRPV SESDYKAIES AMQELVKQKM PYQRLEVSKE DALRLFGYTE FKSKILASKV PDGGSCTVYR CGMLIDPCRG PHLPDTGRVK AFAVTKNSSS YFEGKAENEV LQRVYGISFP KNPMLTEWKT IQEEAAKRDH RVIGRQQNLF NFHEVSPGSA FWLPHGTRIY NTLVEFLRKK YRRCGFQEVI SPNMYSSKLW MVSGHWEKYA DNMFCTKCEK EDFGLKPMNC PGHCIMFASQ PHSYKELPIR YADFGVLHRN ELSGALTGLT RVRRFQQDDA HIFCRMDQIT DEMEGQMQFL SDVYGVLGFK FYFYHSTRPA NKLGSDALWD QSESYLRTAL NRFCGIPEEL PDPLHPDQRF HYDGKPDSVK KMKALMKKAE RCEDPNAWKG PTNGGDGWIE NAGDGAFYGP KIDIVVEDAL RRRHQCATIQ LDFNLPSRFG LKYTLPAAEK DETTVSPTEK QKHADPALTA SSPAVEDCVS KPKPGTYEAA VRDLGIDLEL DANQARPVMI HRAILGSLER SIAILCEHFG GDWPFWLSPR QVMVVPVSAS NYEYSQQVRD TMHDAGFHAD VDNGAATLDK KIRNAEKARY NFILVVGQKE QDATAVNIRA RGEKRLGNKS LVEAVQWLKE LADTHNREY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTGGCAAG AAGAAGGCGG CGGAGGCAAC CGTGGACCTG GCCACCCTGG CGGAGCCAGG CTTCTGGAGG CAACGCGTCG AAATCTTCGA GCAGCTATGG CAGCAGCAGC AGAACCGCTA TGAGTCGATG AAGGCCCCGA TCAAGGTGAG CCTCCCGGAT GGAAAGGTGA TGGACGCCGA GAGCTGGGTT ACGTGCCCGC TGGACATCGC AAAGCGCCTG TCCAACTCGC TGCCAGACAA GGTCATTGTG GCCCGCGTGA ACGAGGCGCT GTGGGACCTG ACGCGGCCGT TCGAGGCTGA CTGTCAACTG GAGCTGCTGG ACTGGGACGA CAAGGATGCG CGTGAGGTGT TCTGGCACAG CTCCTCGCAC GTGCTCGGCT ACGCGCTCGA GCGCATCTTC GACACGAAGC TCTCCGTCGG CCCGGCGCTG GAGGAGGGCG GTTTCTTTTA CGAGGGTCTC ACCAATCGCC CGGTGTCGGA GTCCGACTAC AAGGCGATCG AGTCGGCCAT GCAGGAGCTG GTGAAGCAGA AGATGCCATA CCAGCGTCTC GAAGTGTCGA AGGAGGACGC CCTGAGACTC TTCGGCTACA CGGAGTTCAA GAGCAAGATC CTGGCAAGCA AGGTGCCGGA CGGAGGCTCG TGCACCGTGT ACCGGTGCGG TATGCTGATT GACCCGTGCC GTGGTCCCCA CCTCCCGGAC ACGGGCCGCG TCAAGGCCTT TGCTGTGACC AAGAACTCCT CCTCTTACTT TGAGGGCAAG GCCGAGAACG AGGTGCTGCA GCGCGTGTAC GGCATTTCGT TTCCGAAGAA CCCCATGCTG ACGGAGTGGA AGACGATCCA GGAGGAGGCC GCGAAGCGCG ACCACCGCGT AATTGGCCGC CAGCAGAACC TCTTCAACTT CCACGAGGTC TCACCTGGCA GCGCCTTCTG GCTGCCGCAC GGCACCCGGA TCTACAACAC TCTCGTAGAA TTCCTGCGCA AGAAGTATCG CCGCTGCGGC TTTCAGGAGG TCATCTCGCC GAACATGTAC TCCTCCAAAC TTTGGATGGT GTCGGGCCAC TGGGAGAAGT ACGCCGATAA CATGTTCTGC ACCAAGTGCG AGAAGGAGGA CTTTGGTCTG AAGCCGATGA ACTGCCCGGG CCACTGCATC ATGTTCGCGT CGCAGCCGCA CTCGTACAAG GAGCTGCCGA TTCGCTACGC CGACTTTGGC GTGCTGCACC GGAACGAGCT GAGCGGTGCC TTGACGGGGC TGACCCGCGT GCGCCGCTTC CAGCAGGACG ATGCACACAT CTTCTGCCGC ATGGACCAGA TCACAGATGA GATGGAGGGC CAGATGCAGT TCCTCAGCGA CGTTTACGGC GTGCTGGGCT TCAAGTTCTA CTTTTACCAC TCCACCCGCC CCGCGAACAA GCTCGGCTCT GACGCGCTCT GGGACCAGTC CGAATCGTAT CTGCGCACCG CGCTCAACCG GTTCTGCGGC ATTCCGGAGG AGCTGCCCGA TCCGTTGCAC CCGGACCAGC GCTTTCACTA CGACGGCAAG CCGGACTCGG TCAAGAAGAT GAAGGCGCTC ATGAAGAAGG CGGAGCGCTG CGAGGACCCG AACGCGTGGA AGGGCCCGAC CAACGGCGGC GATGGCTGGA TCGAGAACGC TGGGGACGGC GCCTTCTACG GCCCCAAGAT CGACATCGTC GTCGAGGACG CGCTGCGCCG CCGCCACCAG TGCGCCACGA TCCAGTTGGA CTTCAACCTG CCGAGCCGCT TCGGCCTCAA GTACACCCTT CCCGCCGCAG AGAAGGACGA GACGACGGTG AGCCCAACGG AGAAGCAGAA GCATGCCGAC CCTGCCCTGA CCGCGTCGTC GCCGGCCGTG GAGGATTGCG TGAGCAAACC GAAGCCCGGC ACGTACGAGG CTGCTGTGCG TGACCTTGGC ATCGACCTCG AGCTGGACGC CAACCAGGCC CGCCCCGTCA TGATCCACCG CGCCATTCTC GGTTCCTTGG AGCGCTCGAT CGCCATTCTG TGCGAGCACT TTGGCGGCGA CTGGCCCTTC TGGCTGAGCC CCCGCCAAGT GATGGTGGTG CCTGTTTCGG CCTCCAACTA CGAGTATTCA CAGCAGGTGC GCGACACGAT GCACGATGCC GGCTTCCACG CGGACGTCGA CAACGGTGCC GCCACGCTGG ACAAGAAGAT CCGCAATGCG GAAAAGGCGC GCTACAACTT TATCCTTGTC GTGGGCCAGA AGGAGCAGGA CGCGACCGCC GTCAACATCC GCGCGCGCGG GGAGAAGAGA CTGGGCAACA AGTCACTCGT GGAGGCGGTG CAGTGGCTCA AGGAGCTGGC GGACACACAT AACCGGGAGT ACTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA