LedoB.10201.a

methionyl-tRNA synthetase, putative

CENTER ID: LedoB.10201.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.10201.a.B1.GE42605 full length 1 747
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LdBPK_210890.1
UniProt: E9BF75

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKFFTEKAN HQALKAVLCA VFVQKPLEVT LGSTYSTPYL QLPKSRALLY SCNEAARLLW NTHAAPSPAD NSLEGEAEWL EWESTVLTPA LTPLYTQRRI TGEAEVALKK LDSTIEEHQG TVAVKGAPSS TSFLVDSVLF ASLLPALCEG GLLPAAQAAR VPHLVKWFQV FQAEHAELIA SAFDVLSVQE CSDFLRVPQT YEVSPKKQKV FFATTPIYYV NASPHIGHVY STLIVDVLGR YHRVKGEEVF VMTGTDEHGQ KVAEAAAKQG VSPMDFTTSV SSEFKECFKE MNYDMNYFIR TTNPTHEKLV QDIWKKLEAK GDIYLGKYEG WYSVSDESFL TAQNVADGVD KDGKPCKVSL ESGHVVTWVE EENYMFRLSA FRERLLKYFH DHPNCIVPEF RRREVIKTVE KGLFDLSISR KRESVMNWSI PVPGDERHCI YVWLDALFNY YTGALTRVAA DGTETLDEDH HTLNRWPADV HVVGKDILKF HAIYWPAFLM SAELPLPERL VSHGWWTKDR RKISKSLGNA FDPVEKAKEF GIDALKYFLM RESNFQDDGD YSDKNMVARL NGELADTLGN LVSRCVAPKI NVNGMWPEPA EYSESDKTLI ASLNNLAGTV DHYYCLPDIQ HALIAIFDVL RSLNAYVTEN APWKLVKTDT ARLGTVLYVT MEGLRICTMF LQPVMPQKAK EIMDALGVPE AARVDMENYL FGIVKPRTKI AGLAEGQVIF QKVTLPTEEG ERIPEGQ
NT Sequence
atgctcaagt tttttacaga gaaggcgaat catcaggccc tcaaggccgt gctttgcgcc gtgtttgtgc agaagcccct cgaggtgacg ctgggcagca cctacagcac gccgtatctt cagctgccca agtccagggc gcttctgtac agctgcaatg aggcggcgcg tcttctctgg aacacgcacg ctgcgccgtc gcccgccgac aactcgctcg aaggagaggc ggaatggctc gagtgggagt cgacggtgct gacgccagcc ttgacccccc tctacacgca gcgacgcatc acgggcgagg ctgaggttgc cctgaagaag ctcgactcca ccatcgagga gcaccagggc accgtggcgg tgaagggggc gccgtcgtcc acgtctttct tagtcgacag tgtcctcttc gcgtccctcc tgccggctct gtgcgagggt gggctgctcc ccgccgcgca ggcggcgagg gtgccgcatt tggtgaagtg gttccaggtt ttccaggcgg agcacgcgga gctgattgcc tctgcgtttg acgtgctgtc ggtgcaagag tgcagcgact tcttgcgggt gccgcagacg tacgaggtga gccccaagaa gcagaaagtc ttcttcgcca cgacgccgat ctactacgtg aacgcgtcgc cacacatcgg ccacgtctac agcacgctca tcgtcgacgt tctcggccgt tatcaccgag tgaagggaga ggaggtgttt gtgatgacgg gcacggacga gcacggccag aaggtggccg aggcagcggc gaagcagggg gtatcgccga tggacttcac aacgagcgtg tcgagcgagt ttaaggagtg tttcaaggag atgaattatg acatgaacta cttcatccgc acgacgaacc cgacgcatga gaagctggtg caggacatct ggaagaagct ggaggcgaag ggcgacatct acctcggcaa gtacgagggc tggtactcgg tgagcgacga gtcattcctg acggcgcaga acgtggcgga cggtgtagac aaggatggaa agccgtgcaa ggttagcctg gagagcggcc acgtcgtgac gtgggtggag gaggagaact acatgttccg tcttagcgcg ttccgcgagc gtctcctcaa gtacttccat gaccatccca actgcatcgt cccggagttc cgccgccgcg aggtgatcaa gacagtcgag aagggtctct tcgacttgtc catctcccgc aagcgcgagt ccgtcatgaa ctggtccatc ccggtccccg gtgatgagcg ccactgcatc tacgtgtggc tggatgccct cttcaactac tacaccggcg ccctgacccg tgtcgcagcc gacggcaccg agacactgga cgaggaccac cacacactga accggtggcc ggccgacgtg cacgtggtcg gcaaggacat cctcaagttt catgccattt actggccagc cttcctgatg tccgcggaac tgccgctgcc ggagcggctg gtgtcgcatg gctggtggac aaaagatcgc aggaagatca gcaagtcgct cggcaacgcc ttcgacccag tggaaaaggc gaaagagttc ggcatcgacg cgctcaaata cttcctgatg cgcgagtcga acttccagga tgatggcgat tacagtgaca agaacatggt ggcccgcctg aatggcgaac tcgccgacac gctcggcaac ttggtgtcgc gctgtgtggc gccgaagatc aatgtgaacg gtatgtggcc ggaacccgca gagtacagcg agagcgacaa gacgctgatc gcctcgctca acaacctggc tggcacggtg gaccactatt actgcctgcc tgacattcag cacgcgttga ttgccatctt tgacgtgctc cgcagcctca acgcctatgt gactgagaac gcaccgtgga agctggtgaa aacggacacg gcgcgtctcg gcacagtgct gtatgtaacg atggagggtc tgcgcatctg caccatgttc ctgcagccgg tgatgccgca gaaggcgaag gagattatgg acgcgctcgg ggtgccggag gcggcgcgcg tggacatgga aaactaccta ttcggcattg taaagccgag aacgaagatt gctggcttgg cggagggcca ggtcatcttc cagaaggtga cactccccac tgaggagggg gagcgcattc ccgaaggaca gtaa
Details for LedoB.10201.a.B1.GE42605
HARVESTED ON: 8/5/2018
SEQUENCED ON: 8/8/2018
EXPECTED MW: 85kDa
OBSERVED MW: 85kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML KFFTEKANHQ ALKAVLCAMF VQKPLEVTLG STYSTPYLQL PKSRALLYSC NEAARLLWNT HAAPSPADNS LEGEAEWLEW ESTVLTPALT PLYTQRRVTG EAEVALKKLD STIEEHQGTV AVKGAPSSTS FLVDSVLFAS LLPALCEGGL LPAAQAARVP HLVKWFQVFQ AEHAELIASA FDVLSVQECS DFLRVPQTYE VSPKKQKVFF ATTPIYYVNA SPHIGHVYST LIVDVLGRYH RVKGEEVFVM TGTDEHGQKG GRGSGEAGVS PMDFTTSVSS EFKQCFKEMN YDMNYFIRTT NPTHEKLVQX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXDALFNYYT GALTRVAADG TETLDEDHHT LNRWPADVHV VGKDILKFHA IYWPAFLMSA ELPLPERLVS HGWWTKDRRK ISKSLGNAFD PVEKAKEFGI DALKYFLMRE SNFQDDGDYS DKNMVARLNG ELADTLGNLV SRCVAPKINV NGMWPEPAEY SESDKTLIAS LNNLAGTVDH YYCLPDIQHA LIAIFDVLRS LNAYVTENAP WKLVKTDTAR LGTVLYVTME GLRICTMFLQ PVMPQKAKEI MDALGVPEAA RVDMENYLFG IVKPRTKIAG LAEGQVIFQK VTLPTEXGEX IPEGQ
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHML KFFTEKANHQ ALKAVLCAVF VQKPLEVTLG STYSTPYLQL PKSRALLYSC NEAARLLWNT HAAPSPADNS LEGEAEWLEW ESTVLTPALT PLYTQRRITG EAEVALKKLD STIEEHQGTV AVKGAPSSTS FLVDSVLFAS LLPALCEGGL LPAAQAARVP HLVKWFQVFQ AEHAELIASA FDVLSVQECS DFLRVPQTYE VSPKKQKVFF ATTPIYYVNA SPHIGHVYST LIVDVLGRYH RVKGEEVFVM TGTDEHGQKV AEAAAKQGVS PMDFTTSVSS EFKECFKEMN YDMNYFIRTT NPTHEKLVQD IWKKLEAKGD IYLGKYEGWY SVSDESFLTA QNVADGVDKD GKPCKVSLES GHVVTWVEEE NYMFRLSAFR ERLLKYFHDH PNCIVPEFRR REVIKTVEKG LFDLSISRKR ESVMNWSIPV PGDERHCIYV WLDALFNYYT GALTRVAADG TETLDEDHHT LNRWPADVHV VGKDILKFHA IYWPAFLMSA ELPLPERLVS HGWWTKDRRK ISKSLGNAFD PVEKAKEFGI DALKYFLMRE SNFQDDGDYS DKNMVARLNG ELADTLGNLV SRCVAPKINV NGMWPEPAEY SESDKTLIAS LNNLAGTVDH YYCLPDIQHA LIAIFDVLRS LNAYVTENAP WKLVKTDTAR LGTVLYVTME GLRICTMFLQ PVMPQKAKEI MDALGVPEAA RVDMENYLFG IVKPRTKIAG LAEGQVIFQK VTLPTEEGER IPEGQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctca agttttttac agagaaggcg aatcatcagg ccctcaaggc cgtgctttgc gccgtgtttg tgcagaagcc cctcgaggtg acgctgggca gcacctacag cacgccgtat cttcagctgc ccaagtccag ggcgcttctg tacagctgca atgaggcggc gcgtcttctc tggaacacgc acgctgcgcc gtcgcccgcc gacaactcgc tcgaaggaga ggcggaatgg ctcgagtggg agtcgacggt gctgacgcca gccttgaccc ccctctacac gcagcgacgc atcacgggcg aggctgaggt tgccctgaag aagctcgact ccaccatcga ggagcaccag ggcaccgtgg cggtgaaggg ggcgccgtcg tccacgtctt tcttagtcga cagtgtcctc ttcgcgtccc tcctgccggc tctgtgcgag ggtgggctgc tccccgccgc gcaggcggcg agggtgccgc atttggtgaa gtggttccag gttttccagg cggagcacgc ggagctgatt gcctctgcgt ttgacgtgct gtcggtgcaa gagtgcagcg acttcttgcg ggtgccgcag acgtacgagg tgagccccaa gaagcagaaa gtcttcttcg ccacgacgcc gatctactac gtgaacgcgt cgccacacat cggccacgtc tacagcacgc tcatcgtcga cgttctcggc cgttatcacc gagtgaaggg agaggaggtg tttgtgatga cgggcacgga cgagcacggc cagaaggtgg ccgaggcagc ggcgaagcag ggggtatcgc cgatggactt cacaacgagc gtgtcgagcg agtttaagga gtgtttcaag gagatgaatt atgacatgaa ctacttcatc cgcacgacga acccgacgca tgagaagctg gtgcaggaca tctggaagaa gctggaggcg aagggcgaca tctacctcgg caagtacgag ggctggtact cggtgagcga cgagtcattc ctgacggcgc agaacgtggc ggacggtgta gacaaggatg gaaagccgtg caaggttagc ctggagagcg gccacgtcgt gacgtgggtg gaggaggaga actacatgtt ccgtcttagc gcgttccgcg agcgtctcct caagtacttc catgaccatc ccaactgcat cgtcccggag ttccgccgcc gcgaggtgat caagacagtc gagaagggtc tcttcgactt gtccatctcc cgcaagcgcg agtccgtcat gaactggtcc atcccggtcc ccggtgatga gcgccactgc atctacgtgt ggctggatgc cctcttcaac tactacaccg gcgccctgac ccgtgtcgca gccgacggca ccgagacact ggacgaggac caccacacac tgaaccggtg gccggccgac gtgcacgtgg tcggcaagga catcctcaag tttcatgcca tttactggcc agccttcctg atgtccgcgg aactgccgct gccggagcgg ctggtgtcgc atggctggtg gacaaaagat cgcaggaaga tcagcaagtc gctcggcaac gccttcgacc cagtggaaaa ggcgaaagag ttcggcatcg acgcgctcaa atacttcctg atgcgcgagt cgaacttcca ggatgatggc gattacagtg acaagaacat ggtggcccgc ctgaatggcg aactcgccga cacgctcggc aacttggtgt cgcgctgtgt ggcgccgaag atcaatgtga acggtatgtg gccggaaccc gcagagtaca gcgagagcga caagacgctg atcgcctcgc tcaacaacct ggctggcacg gtggaccact attactgcct gcctgacatt cagcacgcgt tgattgccat ctttgacgtg ctccgcagcc tcaacgccta tgtgactgag aacgcaccgt ggaagctggt gaaaacggac acggcgcgtc tcggcacagt gctgtatgta acgatggagg gtctgcgcat ctgcaccatg ttcctgcagc cggtgatgcc gcagaaggcg aaggagatta tggacgcgct cggggtgccg gaggcggcgc gcgtggacat ggaaaactac ctattcggca ttgtaaagcc gagaacgaag attgctggct tggcggaggg ccaggtcatc ttccagaagg tgacactccc cactgaggag ggggagcgca ttcccgaagg acagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat