LedoB.01593.a

Protein kinase, putative

CENTER ID: LedoB.01593.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
LedoB.01593.a.HZ2.GE43607 cSMRT Kinase domain 4 303
LedoB.01593.a.HZ3.GE43712 kinase domain (Jensen) 1 337
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LdBPK_270100.1
UniProt: E9BIU5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDKYEILAQI GDGTFGSVAK AVSKKTGQLV AIKKMKQKFY TWEECVKLPE VDVVRRIHGH PNVVKLREVI RENNELFFVF EYMDGDLLGV IKKAKQQGGP PASTPSAAPL IPYPLVKNYM RQMLQALVYI HKRGYFHRDM KPENLLIRKE ASGDEVLKLA DFGLVKEICA RPPFTDYVST RWYRAPELLL QDRFYGAAVD VWAAGCIMAE LITMRPLFPG TNEVDQLFKI MSVLGSPTEE VWAGGLRLAK KIRYTFPKVA GSGLAQALPS HIPLPALDLL RQMLVYDPKV RLTAEQCLQH PFFNVGIDEC NAPSAAALDQ LALMAKRMLP GSKTAPPALK SPTAGQVAAL KAARASTDAS LSDTSSRKFY LLGTLKDPAP SLPKLPVALS TSPPSLDAPP NASSPLRGRG ALSPAGMPVL ANGHQCPSLT TTVAKGKRRS QETADTSLAK KLAGLKNVPT GKGKLPVSYA LPAASAVHSP RAAPNLKVSA SVARPYPLAP TANNRDAVAS PRHHNPYKEA ETTSPTAASA AAKVEIDLDE LMEEFASEMT AMGVLAQRHD SGASAESKSE SYVSQPALPP DPVATLLNNS RYRRKSTTSA DPLLKGNNSP RATHQLDTRK VDDVAAKSDL PRIDHSAPKG MSPSLKALLA KHKTSGLAFP
NT Sequence
atggacaagt atgaaattct cgctcaaatc ggagatggca cgttcggctc cgtcgcaaag gccgtgagca agaaaaccgg ccagcttgta gccatcaaga agatgaagca gaaattctac acgtgggagg agtgcgtcaa gcttcccgag gtggacgttg tgcggcgcat tcacggccac cccaacgtgg tgaagctgcg tgaggtaatt cgtgagaaca acgagctgtt tttcgtcttc gagtacatgg acggcgattt gctcggcgtc atcaaaaagg ccaagcagca gggcgggccg ccagctagta caccgtctgc tgcaccgttg attccgtacc ctcttgtaaa gaactacatg cgccaaatgc tgcaggccct cgtgtacatc cacaagcgcg gctacttcca ccgagacatg aagccagaaa accttctcat tcggaaggag gcgagcggcg acgaggtgct gaaactggca gacttcggac tcgtcaagga gatttgtgct cgccccccgt tcaccgacta cgtgtcgacg cggtggtacc gcgcaccaga gctgctcttg caggatcgct tctacggcgc tgcggtggat gtgtgggctg ccgggtgcat tatggcggaa ctcatcacca tgcggccact ttttccaggc acaaacgagg tcgatcagct cttcaagata atgtcagtgc ttgggtcccc cactgaggag gtgtgggccg gtggacttcg cttggccaaa aaaatccgct acacctttcc taaagtagcc ggctccggcc ttgctcaagc gctgccatcg cacatcccgt tgccggcgct ggacttgctg cgccagatgc ttgtctacga cccgaaagta agactgacgg cggagcaatg cctgcagcac ccattcttca acgtcggcat tgacgagtgc aacgcaccat ctgctgccgc gctggaccag ctggcgctca tggcaaagcg gatgctgcca gggtcgaaaa cagcaccgcc ggcgctgaag agccccaccg cgggccaggt ggcggcgctg aaggcggcta gggcctccac cgacgcatca ctctcggaca cctcctcacg caagttctac ctgctcggca ccctcaagga ccccgcccca tcgttgccaa agctcccggt cgcgttgtcc acctcgccac cgtcccttga cgcgccgccg aacgcttcgt cacctctgcg tgggcgcggg gccctctcac cagcggggat gccagtgctc gccaacggcc accagtgccc cagcctgaca acgacggtgg ccaagggaaa gcgccgctcg caggaaacag cagacacctc gcttgcgaaa aagctagcag gcttgaaaaa cgttccgaca ggaaaaggga agctaccggt gtcttacgca ctgcctgctg ccagcgccgt gcactcacct agagcagcac ccaacctgaa ggtctccgca tccgtggcga ggccataccc cctggcgccc accgccaaca accgcgacgc cgtggcgtcc ccgcggcacc acaacccgta caaggaggcc gagactacgt ctccgacggc ggcgtccgcg gcggcgaagg tagagattga cctggacgag ctcatggagg agtttgcgtc ggagatgacc gccatggggg tgctggcgca gcgacacgac tcgggggcct cggccgaatc gaaaagcgaa agctacgttt cccagccagc gcttccgccc gatcctgtgg cgacgctgct gaacaacagc cggtatagaa ggaaatccac cacaagcgcc gacccgctgt tgaaggggaa caactcgcct cgcgctacac accagctgga tacaaggaaa gtggacgatg ttgccgcgaa gagcgacctt cctcgcatag accactcggc cccgaaaggg atgtcgccat cgctgaaggc gcttctagcg aagcacaaga cgagcggtct ggcatttccg tag
Details for LedoB.01593.a.HZ2.GE43607
HARVESTED ON: 2/3/2020
SEQUENCED ON: 2/13/2020
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 62
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMY EILAQIGDGT FGSVAKAVSK KTGQLVAIKK MKQKFYTWEE CVKLPEVDVV RRIHGHPNVV KLREVIRENN ELFFVFEYMD GDLLGVIKKX KQXGGPPAST PSAAPLXPYP LVKNYMRQML XXLVYXXXRG YFHRDMKPEN LX
Validated NT Sequence
atggctcatc accatcacca tcatatgaag tcggactcag aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg ataacgatat tattgaggct cacagagaac agattggtgg taccctggaa gctcagaccc agggtcctgg ttcgatgtat gaaattctcg ctcaaatcgg agatggcacg ttcggctccg tcgcaaaggc cgtgagcaag aaaaccggcc agcttgtagc catcaagaag atgaagcaga aattctacac gtgggaggag tgcgtcaagc ttcccgaggt ggacgttgtg cggcgcattc acggccaccc caacgtggtg aagctgcgtg aggtaattcg tgagaacaac gagctgtttt tcgtcttcga gtacatggac ggcgatttgc tcggcgtcat caaaaaggnc aagcagcang gcgggccgcc agctagtaca ccgtctgctg caccgttgan tccgtaccct cttgtaaaga actacatgcg ccaaatgctg nnngncctcg tgtacnnncn cnagcgcggc tacttccacc gagacatgaa gccngaaaac ctcncattc
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSYE ILAQIGDGTF GSVAKAVSKK TGQLVAIKKM KQKFYTWEEC VKLPEVDVVR RIHGHPNVVK LREVIRENNE LFFVFEYMDG DLLGVIKKAK QQGGPPASTP SAAPLIPYPL VKNYMRQMLQ ALVYIHKRGY FHRDMKPENL LIRKEASGDE VLKLADFGLV KEICARPPFT DYVSTRWYRA PELLLQDRFY GAAVDVWAAG CIMAELITMR PLFPGTNEVD QLFKIMSVLG SPTEEVWAGG LRLAKKIRYT FPKVAGSGLA QALPSHIPLP ALDLLRQMLV YDPKVRLTAE QCLQHPFF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcgt atgaaattct cgctcaaatc ggagatggca cgttcggctc cgtcgcaaag gccgtgagca agaaaaccgg ccagcttgta gccatcaaga agatgaagca gaaattctac acgtgggagg agtgcgtcaa gcttcccgag gtggacgttg tgcggcgcat tcacggccac cccaacgtgg tgaagctgcg tgaggtaatt cgtgagaaca acgagctgtt tttcgtcttc gagtacatgg acggcgattt gctcggcgtc atcaaaaagg ccaagcagca gggcgggccg ccagctagta caccgtctgc tgcaccgttg attccgtacc ctcttgtaaa gaactacatg cgccaaatgc tgcaggccct cgtgtacatc cacaagcgcg gctacttcca ccgagacatg aagccagaaa accttctcat tcggaaggag gcgagcggcg acgaggtgct gaaactggca gacttcggac tcgtcaagga gatttgtgct cgccccccgt tcaccgacta cgtgtcgacg cggtggtacc gcgcaccaga gctgctcttg caggatcgct tctacggcgc tgcggtggat gtgtgggctg ccgggtgcat tatggcggaa ctcatcacca tgcggccact ttttccaggc acaaacgagg tcgatcagct cttcaagata atgtcagtgc ttgggtcccc cactgaggag gtgtgggccg gtggacttcg cttggccaaa aaaatccgct acacctttcc taaagtagcc ggctccggcc ttgctcaagc gctgccatcg cacatcccgt tgccggcgct ggacttgctg cgccagatgc ttgtctacga cccgaaagta agactgacgg cggagcaatg cctgcagcac ccattcttct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for LedoB.01593.a.HZ3.GE43712
HARVESTED ON: data unavailable
SEQUENCED ON: 6/23/2020
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 89
PERCENT COVERAGE: 48
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AXTQGPGSMD KYEILAXXXD GTFGSVAKAV SKKTGQLVAI KKMKQKFYTW EECVKLPEVD VVRRIHGHPN VVKLREXIRE NNELXFRLRV HGRRFARRHQ KGQAAGRAAS
Validated NT Sequence
anngcggtgc tgttttcgac cctggcagca tccgctttgc catgagcgcc agctggtcca gcgcggcagc agatggtgcg ttgcactcgt caatgccgac gttgaagaat gggtgctgca ggcattgctc cgccgtcagt cttactttcg ggtcgtagac aagcatctgg cgcagcaagt ccagcgccgg caacgggatg tgcgatggca gcgcttgagc aaggccggag ccggctactt taggaaaggt gtagcggatt tttttggcca agcgaagtcc accggcccac acctcctcag tgggggaccc aagcactgac attatcttga agagctgatc gacctcgttt gtgcctggaa aaagtggccg catggtgatg agttccgcca taatgcaccc ggcagcccac acatccaccg cagcgccgta gaagcgatcc tgcaagagca gctctggtgc gcggtaccac cgcgtcgaca cgtagtcggt gaacgggggg cgagcacaaa tctccttgac gagtccgaag tctgccagtt tcagcacctc gtcgccgctc gcctccttcc gaatgagaag gttttctggc ttcatgtctc ggtggaagta gccgcgcttg tggatgtaca cgagggcctg cagcatttgg cgcatgtagt ttctttacaa gagggtacgg aatcaacggt gcagcagacg gtgtactagc tggcggcccg ccctgctgct tgnccttttt gatgacgccg agcaaatcgc cgtccatgta ctcgaagacg aaaaancagc tcgttgttct cacgaatnnn ctcacgcagc ttcaccacgt tggggtggcc gtgaatgcgc cgcacaacgt ccacctcggg aagcttgacg cactcctccc acgtgtagaa tttctgcttc atcttcttga tggctacaag ctggccggtt ttcttgctca cggcctttgc gacggagccg aacgtgccat ctcngnnnng agcgagaatt tcatacttgt ccatcgaacc aggaccctgg gtntgagctt ccagggtacc accaatctgt tctctgtgag cctcaataat atcgttatcc tccatgtcca aatcttcagg ggtctgatca gcttgaattc taataccgtc gtacaagaat cttaaggagt ccatttcctt accctgtctt ttagcgaacg cttccatcag ccttcttaaa ggagtggtct ttttgatctt gaagaagatc tctgaagatc catcggacac ctttaaattg atgtgagtct caggcttgac ttctggcttg acctctggct tagcttcttg attgacttct gagtccgact tcatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMD KYEILAQIGD GTFGSVAKAV SKKTGQLVAI KKMKQKFYTW EECVKLPEVD VVRRIHGHPN VVKLREVIRE NNELFFVFEY MDGDLLGVIK KAKQQGGPPA STPSAAPLIP YPLVKNYMRQ MLQALVYIHK RGYFHRDMKP ENLLIRKEAS GDEVLKLADF GLVKEICARP PFTDYVSTRW YRAPELLLQD RFYGAAVDVW AAGCIMAELI TMRPLFPGTN EVDQLFKIMS VLGSPTEEVW AGGLRLAKKI RYTFPKVAGS GLAQALPSHI PLPALDLLRQ MLVYDPKVRL TAEQCLQHPF FNVGIDECNA PSAAALDQLA LMAKRMLPGS KTAPP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tggacaagta tgaaattctc gctcaaatcg gagatggcac gttcggctcc gtcgcaaagg ccgtgagcaa gaaaaccggc cagcttgtag ccatcaagaa gatgaagcag aaattctaca cgtgggagga gtgcgtcaag cttcccgagg tggacgttgt gcggcgcatt cacggccacc ccaacgtggt gaagctgcgt gaggtaattc gtgagaacaa cgagctgttt ttcgtcttcg agtacatgga cggcgatttg ctcggcgtca tcaaaaaggc caagcagcag ggcgggccgc cagctagtac accgtctgct gcaccgttga ttccgtaccc tcttgtaaag aactacatgc gccaaatgct gcaggccctc gtgtacatcc acaagcgcgg ctacttccac cgagacatga agccagaaaa ccttctcatt cggaaggagg cgagcggcga cgaggtgctg aaactggcag acttcggact cgtcaaggag atttgtgctc gccccccgtt caccgactac gtgtcgacgc ggtggtaccg cgcaccagag ctgctcttgc aggatcgctt ctacggcgct gcggtggatg tgtgggctgc cgggtgcatt atggcggaac tcatcaccat gcggccactt tttccaggca caaacgaggt cgatcagctc ttcaagataa tgtcagtgct tgggtccccc actgaggagg tgtgggccgg tggacttcgc ttggccaaaa aaatccgcta cacctttcct aaagtagccg gctccggcct tgctcaagcg ctgccatcgc acatcccgtt gccggcgctg gacttgctgc gccagatgct tgtctacgac ccgaaagtaa gactgacggc ggagcaatgc ctgcagcacc cattcttcaa cgtcggcatt gacgagtgca acgcaccatc tgctgccgcg ctggaccagc tggcgctcat ggcaaagcgg atgctgccag ggtcgaaaac agcaccgccg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa