LedoB.01480.a

Protein kinase, putative

CENTER ID: LedoB.01480.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.01480.a.HZ2.GE43606 cSMRT Kinase domain 357 639
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LdBPK_312960.1
UniProt: E9BN66

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEAAPAAAAQ RPPQTQKRPR EESNAPLQEG SNGSVPATFS SEASVTTPVA DTELVVDVDQ PETNPQAPAV DAPRSRSRGL AAVGPGSISP VAASRSGTDG ANNCQPAAAV TTLSSSSASA DARRFKQPTL AAYGMVSDTM ALKKEISDRD SHIEELNREV EGLRAEVGRR NDQIHFMDGQ CRQYQKKLEH FQSVMRQEML SAAQKDRSEA RRVLYQKHFE LGQTATWHSN GQTVWMEGDR VRTLFLKLAN LTQKREEVEA LKKAAQSNVK HLARQERERE DSGAGPDLQD ALMAAQMEYQ LRASEHAALT NTIADIKLKQ TEIEHEKKAF VKEMRRVNDE DTSEFLAIPT IGEGDRYVLM HLLGKGGFSE VWKAFDLVEG RYVACKIHHI QREWPAQTRT HYLRHAQREL DIMRALDHPH LTHLYDVFPR GDNMFISVME YSNGMDLDTY LKRYRTFKEA DARLIFLQVV DVLRYLASLD SPIIHYDLKP ANILLHRDDP TILDIKITDF GLSKIIGATR EGPSDNPSIE LTSQGTGTYW YLPPECFETS STPRISNKVD IWSAGVIFYQ MLFGKRPFAE GESQRRIWQE KLIIQSARTL HFPDTPKVSE EAKDIIRGCL AFNERERCDV FQLSQDPYLF RTSRRSAHKA KSAAPSGVGS SNSPLMPPVT DATPAS
NT Sequence
atggaggccg cccccgctgc cgctgcgcag cgaccgccgc agacccaaaa gcggccccgc gaggaaagca acgccccgct acaggaaggt agcaacggca gcgtgcccgc taccttctcc agcgaggcct ccgtcaccac gcctgttgcc gacacggagc tcgtcgtcga cgtcgatcag ccagagacca acccgcaggc gccggccgtt gacgccccgc gttcacgatc gcgtggacta gccgccgtcg ggccaggctc catctcaccc gtcgcggcct cccgcagcgg cactgacggt gccaataact gccagccggc tgcagcggtc acaacactgt cgtcgtcttc ggcatctgcg gacgcgcgcc gcttcaagca gcccaccctt gccgcctacg gcatggtcag cgacacgatg gcgctcaaga aggaaatcag cgaccgtgac agtcacatcg aggagcttaa tcgcgaggtg gaagggctgc gtgccgaggt ggggcggcgc aatgaccaga tacacttcat ggacggtcaa tgtcggcagt accagaagaa actggagcac ttccagtctg tcatgcggca ggagatgcta agcgctgccc agaaggaccg ctcggaggca cggcgcgtcc tctatcagaa acatttcgag ctcggccaga ccgcgacgtg gcacagcaac ggccagactg tatggatgga gggcgatcga gtgcgcacgc tgttcttgaa gctggccaac ctcactcaaa aacgtgagga ggtcgaggcg ctcaagaagg cggcgcagag caacgtgaag cacctggccc gccaggagcg cgagcgggag gactctggcg ccggcccgga cctgcaggac gcgctgatgg ctgcccagat ggagtatcag ctgcgcgcgt ccgagcacgc cgctctgacc aacaccatcg ccgacatcaa gctgaagcag acagagatcg agcacgagaa gaaggccttc gtgaaggaga tgcgccgcgt gaatgacgag gacacgtccg agttcttggc gatccccacc atcggcgagg gagaccgcta cgtcctcatg catctactag gcaagggtgg cttctcggaa gtgtggaagg cgttcgacct cgtcgaaggc cgctatgtgg catgtaagat tcaccacatt cagcgcgagt ggcctgccca gacgcgtact cactacctgc gccatgcgca gcgggagctc gacatcatgc gcgcgctcga tcacccgcat ctgacgcacc tctacgacgt cttccctcgc ggtgacaaca tgttcatctc cgtgatggag tacagcaacg gcatggacct cgacacctac ctcaagcgct accgcacctt caaggaggcc gacgcgcggc tcatctttct gcaggtcgtg gacgtgctgc gctacctcgc ctccctcgac agccctatca tccactacga cctaaagccg gccaacatcc tgttgcaccg cgacgaccca accatcctcg acatcaagat taccgacttt ggcctctcca agatcatcgg cgccacccgc gaaggcccca gcgacaaccc gtccatcgag ctcacctcgc agggcaccgg cacgtactgg tacctgccgc ccgagtgctt cgaaacctcc tcgacaccgc gcatcagcaa caaagtcgac atctggtccg ccggcgtcat cttctaccag atgctctttg gcaagcggcc attcgccgag ggcgagtcgc agcgccgcat atggcaggaa aagctcatca ttcaatccgc gcgcacactg cacttcccag acacacccaa ggtaagcgag gaggccaagg acatcatccg tggctgcctc gccttcaacg aacgcgagcg atgcgacgtg tttcagctga gtcaagaccc ctacctgttt cgcacgagcc gccgctctgc gcacaaggcg aaaagcgccg ctccctctgg cgtcggaagc tcgaactcac cgctgatgcc acccgttacc gacgcaacac cggcatcata g
Details for LedoB.01480.a.HZ2.GE43606
HARVESTED ON: 2/3/2020
SEQUENCED ON: 2/13/2020
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 79
PERCENT COVERAGE: 33
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THXXLKVSDG SSEIFFXIXK TXPXXRLMEA XXKRQGKEMD SXXFLYDGIX IQADXTPEDL DMEDNDIIEA HXXXIGGTLX XXTQGPGXMX XLMXLXXKGG FXXXW
Validated NT Sequence
atggctcatc accatcacca tcatatgaag tcggactcag aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacntnn ntttaaaggt gtccgatgga tcttcagaga tcttcttcaa natcnnaaag accncnccnn tnanaaggct gatggaagcg ntcnctaaaa gacagggnaa ggaaatggac tcctnnnnat tcttgtacga cggtattana attcaagctg atcanacccc tgaagatttg gacatggagg ataacgatat tattgaggct cacananaac anattggngg taccctgnan nctcnnaccc agggtcctgg nncnatgtnc nnnctcatgn ntctacnnng caagggnggc ttcnnnnaan tgtggaa
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSYV LMHLLGKGGF SEVWKAFDLV EGRYVACKIH HIQREWPAQT RTHYLRHAQR ELDIMRALDH PHLTHLYDVF PRGDNMFISV MEYSNGMDLD TYLKRYRTFK EADARLIFLQ VVDVLRYLAS LDSPIIHYDL KPANILLHRD DPTILDIKIT DFGLSKIIGA TREGPSDNPS IELTSQGTGT YWYLPPECFE TSSTPRISNK VDIWSAGVIF YQMLFGKRPF AEGESQRRIW QEKLIIQSAR TLHFPDTPKV SEEAKDIIRG CLAFNERERC DVFQLSQDPY L
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcgt acgtcctcat gcatctacta ggcaagggtg gcttctcgga agtgtggaag gcgttcgacc tcgtcgaagg ccgctatgtg gcatgtaaga ttcaccacat tcagcgcgag tggcctgccc agacgcgtac tcactacctg cgccatgcgc agcgggagct cgacatcatg cgcgcgctcg atcacccgca tctgacgcac ctctacgacg tcttccctcg cggtgacaac atgttcatct ccgtgatgga gtacagcaac ggcatggacc tcgacaccta cctcaagcgc taccgcacct tcaaggaggc cgacgcgcgg ctcatctttc tgcaggtcgt ggacgtgctg cgctacctcg cctccctcga cagccctatc atccactacg acctaaagcc ggccaacatc ctgttgcacc gcgacgaccc aaccatcctc gacatcaaga ttaccgactt tggcctctcc aagatcatcg gcgccacccg cgaaggcccc agcgacaacc cgtccatcga gctcacctcg cagggcaccg gcacgtactg gtacctgccg cccgagtgct tcgaaacctc ctcgacaccg cgcatcagca acaaagtcga catctggtcc gccggcgtca tcttctacca gatgctcttt ggcaagcggc cattcgccga gggcgagtcg cagcgccgca tatggcagga aaagctcatc attcaatccg cgcgcacact gcacttccca gacacaccca aggtaagcga ggaggccaag gacatcatcc gtggctgcct cgccttcaac gaacgcgagc gatgcgacgt gtttcagctg agtcaagacc cctacctgta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a