LedoB.01391.a

Mitogen-activated protein kinase (EC 2.7.11.24)

CENTER ID: LedoB.01391.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.01391.a.HZ2.GE43876 cDMRT domain 13 302
LedoB.01391.a.HZ3.GE43710 kinase domain (Jensen) 1 359
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LdBPK_360780.1
UniProt: E9BTD7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAEIESHIV KKYEIQAQLG QGAYGIVWRA LERKHNRVVA LKKIYDAFQN STDAQRTFRE IMFLHRLHHP NIIKLLHVHR AFNDRDIYLV FEYMETDLHV VIRANILEEI HKQFIIYQLL KTMKYLHSAE ILHRDMKPSN LLVNSDCTMK VADFGLARSI LSLEGEQASR PVLTDYIATR WYRPPEILLG STRYTKGVDM WSVGCILGEL MLGKPIFPGR STTNQLELIC SVTGMPSAAD VAATNSQFAH AMLRDIHCAH RRTFAELLPS ASADALDLIE RLMCFNPNRR MTAAEALEHP YVAAFHRPDD EPVATEPITV SLPDSQRLPL AKYRDAIYEQ IAALRRSSTS ADQRQRAERQ AAGSTASRKT SVSSASAGGS RGGTGTSGVT RPATSSSSSA AAGAAPQRSV VKPTSTSAVN EASSSKAYAR PAFRSATSAA SGLESRPVAR EAAVRK
NT Sequence
atgtcggccg aaatcgagtc gcatattgtg aagaagtatg agattcaagc acagctagga cagggtgcct acggcatcgt ttggcgcgcc ctggagcgca aacacaaccg cgtcgttgca ctcaagaaaa tttacgacgc cttccaaaac tcgaccgatg cacagcgcac cttccgcgag atcatgtttc tgcaccggct gcaccacccg aacatcatca agctgctgca cgttcatcgt gccttcaacg atcgcgacat atacctggtc ttcgaataca tggagaccga cctgcacgtg gtgattaggg caaacatctt ggaggaaatt cacaaacagt tcatcatcta ccagctgctc aagacgatga agtacctgca ttctgctgag attcttcacc gtgatatgaa accaagcaat ctgctcgtaa acagcgactg cacaatgaaa gtggctgact tcggtctcgc gcgctctatc ctgtcccttg agggtgagca ggcgtcgcgg ccggtgctaa cggactacat tgcgacacgg tggtaccgcc cgccggagat tttgctcggc tcgacacgat acactaaggg cgtggatatg tggtctgtgg gctgcattct tggggagttg atgctgggca agcccatctt tcccggccga tccaccacga accagttgga gctgatctgc agcgtgacgg gcatgccctc cgcggcggat gtggcggcga ccaactcgca gtttgcccac gcgatgcttc gcgacattca ctgcgcgcat cgacgcacgt tcgctgagct gctgccaagc gcttctgccg acgcgctgga cctgatcgag cgccttatgt gtttcaaccc aaaccgtcgc atgactgccg ccgaggccct ggagcacccg tacgtcgctg cctttcaccg tcctgacgac gaaccggtgg cgaccgaacc catcaccgtc tctctccccg acagccagcg tctgcccttg gccaagtaca gggacgccat ctacgagcaa attgccgcgc tgcgccgtag ctccacgtcc gccgatcaac gacagcgcgc ggagcgacag gccgccggca gcaccgccag ccgcaagacg tccgtcagca gcgcctccgc tggcggctct cgagggggca ccggtacatc tggggtcacg cgacccgcca cgagcagcag cagcagcgcc gccgcagggg cggctccgca gcgcagtgtt gtgaagccca cctcaaccag cgcggtcaac gaggcatcat cttcgaaggc gtacgcccgc cccgccttcc gctcggcaac gtctgccgcc tctggtctgg aaagcagacc cgtcgcgcgt gaggcagcgg tgcgcaagtg a
Details for LedoB.01391.a.HZ2.GE43876
HARVESTED ON: 11/2/2020
SEQUENCED ON: 12/2/2020
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMY EIQAQLGQGA YGIVWRALER KHNRVVALKK IYDAFQNSTD AQRTFREIMF LHRLHHPNII KLLHVHRAFN DRDIYLVFEY METDLHVVIR ANILEEIHKQ FIIYQLLKTM KYLHSAEILH RDMKPSNLLV NSDCTMKVAD FGLARSILSL EGEQASRPVL TDYIATRWYR PPEILLGSTR YTKGVDMWSV GCILGELMLG KPIFPGRSTT NQLELICSVT GMPSAADVAA TNSQFAHAML RDIHCAHRRT FAELLPSASA DALDLIERLM CFNPNRRMTA AEALEHP
Validated NT Sequence
atggctcatc accatcacca tcatatgaag tcggactcag aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg ataacgatat tattgaggct cacagagaac agattggtgg taccctggaa gctcagaccc agggtcctgg ttcgatgtat gagattcaag cacagctagg acagggtgcc tacggcatcg tttggcgcgc cctggagcgc aaacacaacc gcgtcgttgc actcaagaaa atttacgacg ccttccaaaa ctcgaccgat gcacagcgca ccttccgcga gatcatgttt ctgcaccggc tgcaccaccc gaacatcatc aagctgctgc acgttcatcg tgccttcaac gatcgcgaca tatacctggt cttcgaatac atggagaccg acctgcacgt ggtgattagg gcaaacatct tggaggaaat tcacaaacag ttcatcatct accagctgct caagacgatg aagtacctgc attctgctga gattcttcac cgtgatatga aaccaagcaa tctgctcgta aacagcgact gcacaatgaa agtggctgac ttcggtctcg cgcgctctat cctgtccctt gagggtgagc aggcgtcgcg gccggtgcta acggactaca ttgcgacacg gtggtaccgc ccgccggaga ttttgctcgg ctcgacacga tacactaagg gcgtggatat gtggtctgtg ggctgcattc ttggggagtt gatgctgggc aagcccatct ttcccggccg atccaccacg aaccagttgg agctgatctg cagcgtgacg ggcatgccct ccgcggcgga tgtggcggcg accaactcgc agtttgccca cgcgatgctt cgcgacattc actgcgcgca tcgacgcacg ttcgctgagc tgctgccaag cgcttctgcc gacgcgctgg acctgatcga gcgccttatg tgtttcaacc caaaccgtcg catgactgcc gccgaggccc tggagcaccc gt
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSYE IQAQLGQGAY GIVWRALERK HNRVVALKKI YDAFQNSTDA QRTFREIMFL HRLHHPNIIK LLHVHRAFND RDIYLVFEYM ETDLHVVIRA NILEEIHKQF IIYQLLKTMK YLHSAEILHR DMKPSNLLVN SDCTMKVADF GLARSILSLE GEQASRPVLT DYIATRWYRP PEILLGSTRY TKGVDMWSVG CILGELMLGK PIFPGRSTTN QLELICSVTG MPSAADVAAT NSQFAHAMLR DIHCAHRRTF AELLPSASAD ALDLIERLMC FNPNRRMTAA EALEHPYV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcgt atgagattca agcacagcta ggacagggtg cctacggcat cgtttggcgc gccctggagc gcaaacacaa ccgcgtcgtt gcactcaaga aaatttacga cgccttccaa aactcgaccg atgcacagcg caccttccgc gagatcatgt ttctgcaccg gctgcaccac ccgaacatca tcaagctgct gcacgttcat cgtgccttca acgatcgcga catatacctg gtcttcgaat acatggagac cgacctgcac gtggtgatta gggcaaacat cttggaggaa attcacaaac agttcatcat ctaccagctg ctcaagacga tgaagtacct gcattctgct gagattcttc accgtgatat gaaaccaagc aatctgctcg taaacagcga ctgcacaatg aaagtggctg acttcggtct cgcgcgctct atcctgtccc ttgagggtga gcaggcgtcg cggccggtgc taacggacta cattgcgaca cggtggtacc gcccgccgga gattttgctc ggctcgacac gatacactaa gggcgtggat atgtggtctg tgggctgcat tcttggggag ttgatgctgg gcaagcccat ctttcccggc cgatccacca cgaaccagtt ggagctgatc tgcagcgtga cgggcatgcc ctccgcggcg gatgtggcgg cgaccaactc gcagtttgcc cacgcgatgc ttcgcgacat tcactgcgcg catcgacgca cgttcgctga gctgctgcca agcgcttctg ccgacgcgct ggacctgatc gagcgcctta tgtgtttcaa cccaaaccgt cgcatgactg ccgccgaggc cctggagcac ccgtacgtct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for LedoB.01391.a.HZ3.GE43710
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 92
PERCENT COVERAGE: 63
Validated AA Sequence
MHSAPSARSC XXTGCXTRTS SKLLHVHRXF NDRDIYLVFE YMETDLHVVI XANILEXIHK QFIIYQLLKT MKYLHSAEIL HRDMKPSNLL VNSDCTMKVA DFGLARSILS LEGEQASRPV LTDYIATRWY RPPEILLGSX XYTKGVDMWS VGCILGELML GKPIFPGRST TNQLELICSV TGMPSAADVA ATNSQFAHAM LRDIHCAHRR TFAELLPSAS ADALDLIERL MCFNPNRRMT AAEALEHPYV AAFHRPDDEP VATEPITVSL PDSQRLPLAK YRDAIYEQIA ALRRSSTSAD QRQRA
Validated NT Sequence
tccgcgcgct gtcgttgatc ggcggacgtg gagctacggc gcagcgcggc aatttgctcg tagatggcgt ccctgtactt ggccaagggc agacgctggc tgtcggggag agagacggtg atgggttcgg tcgccaccgg ttcgtcgtca ggacggtgaa aggcagcgac gtacgggtgc tccagggcct cggcggcagt catgcgacgg tttgggttga aacacataag gcgctcgatc aggtccagcg cgtcggcaga agcgcttggc agcagctcag cgaacgtgcg tcgatgcgcg cagtgaatgt cgcgaagcat cgcgtgggca aactgcgagt tggtcgccgc cacatccgcc gcggagggca tgcccgtcac gctgcagatc agctccaact ggttcgtggt ggatcggccg ggaaagatgg gcttgcccag catcaactcc ccaagaatgc agcccacaga ccacatatcc acgcccttag tgtatcnnnn cgagccgagc aaaatctccg gcgggcggta ccaccgtgtc gcaatgtagt ccgttagcac cggccgcgac gcctgctcac cctcaaggga caggatagag cgcgcgagac cgaagtcagc cactttcatt gtgcagtcgc tgtttacgag cagattgctt ggtttcatat cacggtgaag aatctcagca gaatgcaggt acttcatcgt cttgagcagc tggtagatga tgaactgttt gtgaattnnc tccaagatgt ttgcnctaat caccacgtgc aggtcggtct ccatgtattc gaagaccagg tatatgtcgc gatcgttgaa gncncgatga acgtgcagca gctttgatga tgttcgggtg gngcagccgg tncagnaaca tgatctcgcg gaaggtgcgc tgtgcatcgg tcgagtttng gaaggcgtcg taaattttnn ngagtgcaac gacgcggttg tgtttgcgct ncagggcgcg ccaaacgatg ccgtaggcac cctgtcctag ctgtgcttga atctcatact tcttcagaat atgngactcg atttcggccg acatcgaacc aggaccctgg gtntgagctt ccagggtacc accaatctgt tctctgtgag cctcaataat atcgttatcc tccatgtcca aatcttcagg ggtctgatca gcttgaattc taataccgtc gtacaagaat cttaaggagt ccatttcctt accctgtctt ttagcgaacg cttccatcag ccttcttaaa ggagtggtct ttttgatctt gaagaagatc tctgaagatc catcggacac ctttaaattg atgtgagtct caggcttgac ttctggcttg acctctggct tagcttcttg attgacttct gagtccgact tcatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMS AEIESHIVKK YEIQAQLGQG AYGIVWRALE RKHNRVVALK KIYDAFQNST DAQRTFREIM FLHRLHHPNI IKLLHVHRAF NDRDIYLVFE YMETDLHVVI RANILEEIHK QFIIYQLLKT MKYLHSAEIL HRDMKPSNLL VNSDCTMKVA DFGLARSILS LEGEQASRPV LTDYIATRWY RPPEILLGST RYTKGVDMWS VGCILGELML GKPIFPGRST TNQLELICSV TGMPSAADVA ATNSQFAHAM LRDIHCAHRR TFAELLPSAS ADALDLIERL MCFNPNRRMT AAEALEHPYV AAFHRPDDEP VATEPITVSL PDSQRLPLAK YRDAIYEQIA ALRRSSTSAD QRQRAER
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tgtcggccga aatcgagtcg catattgtga agaagtatga gattcaagca cagctaggac agggtgccta cggcatcgtt tggcgcgccc tggagcgcaa acacaaccgc gtcgttgcac tcaagaaaat ttacgacgcc ttccaaaact cgaccgatgc acagcgcacc ttccgcgaga tcatgtttct gcaccggctg caccacccga acatcatcaa gctgctgcac gttcatcgtg ccttcaacga tcgcgacata tacctggtct tcgaatacat ggagaccgac ctgcacgtgg tgattagggc aaacatcttg gaggaaattc acaaacagtt catcatctac cagctgctca agacgatgaa gtacctgcat tctgctgaga ttcttcaccg tgatatgaaa ccaagcaatc tgctcgtaaa cagcgactgc acaatgaaag tggctgactt cggtctcgcg cgctctatcc tgtcccttga gggtgagcag gcgtcgcggc cggtgctaac ggactacatt gcgacacggt ggtaccgccc gccggagatt ttgctcggct cgacacgata cactaagggc gtggatatgt ggtctgtggg ctgcattctt ggggagttga tgctgggcaa gcccatcttt cccggccgat ccaccacgaa ccagttggag ctgatctgca gcgtgacggg catgccctcc gcggcggatg tggcggcgac caactcgcag tttgcccacg cgatgcttcg cgacattcac tgcgcgcatc gacgcacgtt cgctgagctg ctgccaagcg cttctgccga cgcgctggac ctgatcgagc gccttatgtg tttcaaccca aaccgtcgca tgactgccgc cgaggccctg gagcacccgt acgtcgctgc ctttcaccgt cctgacgacg aaccggtggc gaccgaaccc atcaccgtct ctctccccga cagccagcgt ctgcccttgg ccaagtacag ggacgccatc tacgagcaaa ttgccgcgct gcgccgtagc tccacgtccg ccgatcaacg acagcgcgcg gagcgataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa