SSGCID: Target Page

LedoB.01010.a

Mitogen-activated protein kinase kinase 3, putative

CENTER ID:	LedoB.01010.a
ORGANISM:	Leishmania donovani BPK282A1
ASSOCIATED DISEASE:	Leishmaniasis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LedoB.01010.a.HZ2.GE43604	CSMRT kinase domain		29	285
LedoB.01010.a.HZ3.GE43709	kinase domain (Jensen)		22	345

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LdBPK_170070.1
UniProt:	E9BD34

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDFYALQDTL LRNAKSGGSS DTVDVESRYV LMEKIGAGSY GDVWSATRRT GTKDIYAVKR IDKRQAGTKG LRSVMGEVET MSLLSHPNIV KLEETYQDEA CLSIVMEYLP GGSLQHRIDR QNVSELETRR FITQLLMAVE YIHEKGIVHR DLKPSNCLLS QNDLVVKISD FGLSVFAGNK QCLTTCCGTL HFMAPEILLE KNYGKPVDMW AMGVMAHVMF LGRYPFQART EAALTKDICR GYRPREEGGG GLRCPPLLQD FISQLLLYDP LRRMSAKDAL KHPWIKEGLD ANRRTSLYPS QAPSATAMKP SARWRAAALA VMGVQRLLYL QKIQRLVRLG YDGFPILRDY RYLVTGKYVP ASTSVECSHM FHARPMALLE LISMIDTCPF LKHVDLSWNN IHSLSVIQAL LKVVTRHPSL QSIDLSHNPI PAVAGRSIVR LIRNPLSQVT CINVGDTGIS ADTIGQINSF LKEKLTPAAL SYSVSTGDMQ SRLTQGSGDD TLMTSSSTTM SSLQQKRSIC SGPPKQPAKK ESRGVRLPPI SRPPVARRHG KMP

NT Sequence

atggacttct acgctttgca agacacgctc ttgcgcaatg ccaagagcgg gggcagctct gacaccgtcg acgtcgagtc gaggtacgtc ttgatggaga aaatcggcgc tggctcttat ggcgatgtct ggagcgcgac gcggcgcaca gggacgaagg acatctacgc ggtgaagcgc atcgacaagc gtcaggcggg caccaaggga ctgcggagcg tcatggggga agtggagacg atgagccttc tttctcatcc caatatcgtc aagctcgagg agacgtatca agacgaagcg tgcctatcaa ttgtgatgga gtaccttcct ggtggttccc ttcagcaccg catagaccgg caaaacgtgt ctgagctgga gacgcggcgc ttcatcacgc agctgttgat ggctgtggag tacattcatg aaaagggtat cgtccaccgc gatttgaagc cgagcaactg cctactatct caaaacgacc tcgtcgtaaa gatatcagac ttcgggctct ccgtgtttgc cggcaataag caatgcttga cgacgtgctg tggcacgctg cacttcatgg cacccgagat tctgctagag aagaactacg gcaagccggt ggacatgtgg gccatgggcg tcatggcaca cgtgatgttt ttaggtcgtt acccatttca ggcccgtaca gaggcggcgc tgacgaagga catctgccgc gggtatcgtc cgcgggagga gggtggtggt gggctgcgat gcccaccact gctacaagac tttatcagtc aacttctcct ttacgaccca ctccgtcgaa tgagcgccaa agacgctctc aagcacccat ggatcaagga aggcttagac gccaaccggc ggacgtccct ctacccgtca caggcaccct cggcgacagc gatgaaacca tctgcgcggt ggcgagcagc ggcgcttgca gtcatgggcg tgcagcggct tctctacctg cagaaaattc agcggctagt gcggctcggt tatgacggct ttccaatttt gcgtgactac cgttacctcg taaccggcaa gtatgtacct gcgagcacct ctgtagagtg tagtcacatg ttccacgctc gaccgatggc gctgctcgag ttgatctcca tgatagatac gtgcccgttc ctgaaacatg tagatcttag ctggaataac attcacagct tatctgtaat ccaggcgctg ctcaaggtgg tgacacgaca cccttcgctg caatctattg acctgtctca caatcccatc ccggccgtgg cagggcgttc catcgtgcgg ctgatccgca acccgctttc acaggtgaca tgcatcaacg tcggcgacac cggaatcagt gccgacacca ttgggcagat aaactccttt ttaaaggaga agctgacccc tgccgcgctg tcgtacagcg tctccaccgg agacatgcaa agccgactca cccagggtag tggcgatgac acgctgatga cgtcatcgtc cactacgatg agctccttgc agcaaaagcg tagcatttgt agtgggccac ccaagcagcc cgcgaagaaa gaatcgcgcg gcgtgcggct gcccccgatt tcacgtccac cagtggctag gcgccatggg aagatgcctt ag

Details for LedoB.01010.a.HZ2.GE43604

HARVESTED ON:	2/3/2020
SEQUENCED ON:	2/13/2020
EXPECTED MW:	43kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	96
PERCENT COVERAGE:	72

Validated AA Sequence

MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMY VLMEKIGSGS YGDVWSATRR TGTKDIYAVK RIDKRQAGTK GLRSVMGEVE TMSLLSHPNI VKLEETYQDE ACLSIVMEYL PGGSLQHRID RQNVSELETR RFITQLLMAV EYIHEKGIVH RDLXPXNCLL SQNDLVVKIS XFRALRVCRX

Validated NT Sequence

atggctcatc accatcacca tcatatgaag tcggactcag aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat tcttgtacga cggtattaga attcaagctg atcagacccc tgaagatttg gacatggagg ataacgatat tattgaggct cacagagaac agattggtgg taccctggaa gctcagaccc agggtcctgg ttcgatgtac gtcttgatgg agaaaatcgg ctctggctct tatggcgatg tctggagcgc gacgcggcgc acagggacga aggacatcta cgcggtgaag cgcatcgaca agcgtcaggc gggcaccaag ggactgcgga gcgtcatggg ggaagtggag acgatgagcc ttctttctca tcccaatatc gtcaagctcg aggagacgta tcaagacgaa gcgtgcctat caattgtgat ggagtacctt cctggtggtt cccttcagca ccgcatagac cggcaaaacg tgtctgagct ggagacgcgg cgcttcatca cgcagctgtt gatggctgtg gagtacattc atgaaaaggg tatcgtccac cgcgatttga anccnancaa ctgcctacta tctcaaaacg acctcgtcgt aaagatatca gantttcggg ctctncgngt ttgccggnaa taannnntgn ttgacgacgt gctg

Expected Protein Sequence

MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSYV LMEKIGAGSY GDVWSATRRT GTKDIYAVKR IDKRQAGTKG LRSVMGEVET MSLLSHPNIV KLEETYQDEA CLSIVMEYLP GGSLQHRIDR QNVSELETRR FITQLLMAVE YIHEKGIVHR DLKPSNCLLS QNDLVVKISD FGLSVFAGNK QCLTTCCGTL HFMAPEILLE KNYGKPVDMW AMGVMAHVMF LGRYPFQART EAALTKDICR GYRPREEGGG GLRCPPLLQD FISQLLLYDP LRRMSAKDAL KHPWI

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcgt acgtcttgat ggagaaaatc ggcgctggct cttatggcga tgtctggagc gcgacgcggc gcacagggac gaaggacatc tacgcggtga agcgcatcga caagcgtcag gcgggcacca agggactgcg gagcgtcatg ggggaagtgg agacgatgag ccttctttct catcccaata tcgtcaagct cgaggagacg tatcaagacg aagcgtgcct atcaattgtg atggagtacc ttcctggtgg ttcccttcag caccgcatag accggcaaaa cgtgtctgag ctggagacgc ggcgcttcat cacgcagctg ttgatggctg tggagtacat tcatgaaaag ggtatcgtcc accgcgattt gaagccgagc aactgcctac tatctcaaaa cgacctcgtc gtaaagatat cagacttcgg gctctccgtg tttgccggca ataagcaatg cttgacgacg tgctgtggca cgctgcactt catggcaccc gagattctgc tagagaagaa ctacggcaag ccggtggaca tgtgggccat gggcgtcatg gcacacgtga tgtttttagg tcgttaccca tttcaggccc gtacagaggc ggcgctgacg aaggacatct gccgcgggta tcgtccgcgg gaggagggtg gtggtgggct gcgatgccca ccactgctac aagactttat cagtcaactt ctcctttacg acccactccg tcgaatgagc gccaaagacg ctctcaagca cccatggatc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa

Details for LedoB.01010.a.HZ3.GE43709

HARVESTED ON:	data unavailable
SEQUENCED ON:	6/23/2020
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSTV DVESRYVLME KIGAGSYGDV WSATRRTGTK DIYAVKRIDK RQAGTKGLRS VMGEVETMSL LSHPNIVKLE ETYQDEACLS IVMEYLPGGS LQHRIDRQNV SELETRRFIT QLLMAVEYIH EKGIVHRDLK PSNCLLSQND LVVKISDFGL SVFAGNKQCL TTCCGTLHFM APEILLEKNY GKPVDMWAMG VMAHVMFLGR YPFQARTEAA LTKDICRGYR PREEGGGGLR CPPLLQDFIS QLLLYDPLRR MSAKDALKHP WIKEGLDANR RTSLYPSQAP SATAMKPSAR WRAAALAVMG VQRLLYLQKI QRLVRLGYDG FP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga ccgtcgacgt cgagtcgagg tacgtcttga tggagaaaat cggcgctggc tcttatggcg atgtctggag cgcgacgcgg cgcacaggga cgaaggacat ctacgcggtg aagcgcatcg acaagcgtca ggcgggcacc aagggactgc ggagcgtcat gggggaagtg gagacgatga gccttctttc tcatcccaat atcgtcaagc tcgaggagac gtatcaagac gaagcgtgcc tatcaattgt gatggagtac cttcctggtg gttcccttca gcaccgcata gaccggcaaa acgtgtctga gctggagacg cggcgcttca tcacgcagct gttgatggct gtggagtaca ttcatgaaaa gggtatcgtc caccgcgatt tgaagccgag caactgccta ctatctcaaa acgacctcgt cgtaaagata tcagacttcg ggctctccgt gtttgccggc aataagcaat gcttgacgac gtgctgtggc acgctgcact tcatggcacc cgagattctg ctagagaaga actacggcaa gccggtggac atgtgggcca tgggcgtcat ggcacacgtg atgtttttag gtcgttaccc atttcaggcc cgtacagagg cggcgctgac gaaggacatc tgccgcgggt atcgtccgcg ggaggagggt ggtggtgggc tgcgatgccc accactgcta caagacttta tcagtcaact tctcctttac gacccactcc gtcgaatgag cgccaaagac gctctcaagc acccatggat caaggaaggc ttagacgcca accggcggac gtccctctac ccgtcacagg caccctcggc gacagcgatg aaaccatctg cgcggtggcg agcagcggcg cttgcagtca tgggcgtgca gcggcttctc tacctgcaga aaattcagcg gctagtgcgg ctcggttatg acggctttcc ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa