SSGCID: Target Page

LedoB.00825.b

asparaginyl-tRNA synthetase, putative

CENTER ID:	LedoB.00825.b
ORGANISM:	Leishmania donovani BPK282A1
ASSOCIATED DISEASE:	Leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LedoB.00825.b.B2.GE42672	soluble domain		38	890

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LdBPK_342110.1
UniProt:	E9BR09

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHAKALARLR MHHAARQRMF SVGMWRALWV VAKLTMAEKE APGWQSTIQV NLAMITEKPH ALASPMMASS VRPAVDEAPS FATTSPPLAP GRGFTELSCS VLVPVSLEPP LPATSLPWRD RGLRTGTCAS SSSALVLTAA SSLPSSLRQH WSSCTTLSRC PFFSLALPLC LLRHRRTLPP HHHHRSPRLP LHTPCRTIAI QLHSVLLFKA SGSKGPRAES ERTMENEQQR TAAVAQLKAV VGLDDRGAKD LSSKPERVAD VLAFFAQHSI DEASPREQKV MLFNVWTKVK KPEHRDPVTA FILDGKLDST QKVDAAIRFV NAAGSDVVDT AAMLAECGVG VTVSREDVEK AVAAALVAED VAALKTKWDK NPNMMLGQMR RVAALRWADV EHVRAALERQ VPGLIKDVIV EAKPAAAKKE AAAMPKKEVE SCRQNSNFKD VAQGLPRSPI GELPSHKEGA CVYVVGWAHR VRHQSRMSFV VLRDGTGFIQ CVFDGSTEPF HRESCVAIRG TLRHEPKAKS ELQPPMELHV NEYAVVGDSD GTIETVITAE SSVDKLYDQR HVVVRGTLAS SVLKVRHELL RVFREHFWSH QYYEVTPPTL VQTQVEGGST LFEVLYYGEK AYLTQSSQLY LESVTASLGN VYCCMPSYRA ERSKTKRHLS EFTHLEAEYD VCSFEDLLNH LEGMFCTVIR TVIERVGDLV AMLNPSQLID PNGNVRDPAN YKFTPKRPFR RLRYADAIQF CNENGILNTE TGKPFEFGED ITDQPERAMV AKLGEFVFMT HFPASMKSFY MQRDPDDPTL TESVDVLAPG IGEVLGGSMR MYKYDELLDA YKREGLDAST YYWYTDQRRY GGAPHGGFGL GVERLLVWML NLDSVKDACL YPRYMGRCKP

NT Sequence

atgcatgcca aggccttggc gaggctgcga atgcaccacg ctgcgagaca gcggatgttc tctgtgggca tgtggcgggc gttgtgggtg gtggcgaagc tgacgatggc cgaaaaggag gcgccgggct ggcagtcgac aatacaggtg aatctggcga tgatcacgga aaagccgcat gcgcttgctt ccccgatgat ggcaagtagt gtccggccgg cggtggacga ggcgccctct tttgcaacga cgtcaccgcc tcttgcaccc ggtcgcgggt tcacagagct cagttgctca gttctggtac ccgtctcttt agagccacca ctaccggcga cctccttgcc gtggcgtgat cgaggtttgc gcacgggcac ctgcgcatct tcctcatcag cgctagttct gacggccgcc tcgtctcttc cgtcttctct tcgtcaacac tggtcctctt gcactactct ctcccgctgc cctttcttct ctctggcgtt gccgctctgc ctgctgcgtc accgtcggac cctgccccct caccatcacc accgatcacc gcgtctgccg ctgcacacgc cctgcagaac aatagctatt cagctacact ccgtgttact ctttaaggca tcgggctcga aaggcccacg ggcggaatca gaacgcacca tggagaacga gcagcagcgc accgctgccg ttgcgcagct caaagctgtt gttggccttg acgacagggg cgccaaagac ctgtccagca agccggaacg cgttgccgat gtgctggcct tcttcgcgca gcacagcatc gatgaagctt ctcctcgtga gcagaaggtc atgctgttca acgtgtggac aaaggtgaag aagcctgagc accgcgatcc cgtgaccgcc ttcatccttg acggcaaact cgacagcacg cagaaggtcg acgccgccat caggtttgta aacgcggccg ggagtgatgt ggtggacacg gcggccatgc tggcggagtg cggcgtcggg gtgacggtgt cgcgggagga cgtggagaag gcagttgccg cggccctcgt cgctgaagac gtggccgcgc tcaagacgaa gtgggacaag aacccgaaca tgatgcttgg ccagatgcgc cgtgtggcgg ccctgcgctg ggccgacgtg gagcacgtgc gcgcggcgct ggagaggcag gttccggggt tgatcaagga cgttatcgtg gaggcgaagc cggcagccgc gaagaaggaa gccgctgcta tgccgaagaa ggaggtagag tcgtgccggc agaactcgaa cttcaaggat gttgcgcagg ggctaccgcg ctccccaatt ggcgagctgc cgtcgcacaa agagggcgcc tgcgtctacg tcgttggttg ggcccaccgc gtgcgccacc agtctcgcat gtcctttgtg gtgctgcgcg atggcacggg cttcatccag tgcgtctttg acggctccac ggagcccttc caccgcgaat cgtgcgtcgc cattcgcggg accctgcgtc acgagccgaa agcgaagtcg gagctgcagc cgccgatgga gctgcacgtg aacgagtacg ctgtcgttgg tgactccgac ggtacgattg agacggtcat cacggctgag agctccgttg acaaactgta cgaccagcgc cacgttgtgg tgcgcggcac gctggcgtcg tctgtgctaa aggtccggca cgagctgttg cgcgtgttcc gcgagcactt ctggtcgcac cagtactacg aggtgacgcc gccgacgctg gtgcagacgc aggtggaggg cggctcgacc ctgttcgagg tcctgtacta cggcgagaag gcgtacttga cgcagtcgtc gcagctttac cttgaaagcg tgacggcgtc gctgggcaac gtgtactgct gcatgccgag ctaccgcgcg gagaggtcca agacgaagcg ccacctcagc gagttcacgc acctggaggc cgagtacgac gtgtgctcct ttgaagatct tttgaatcac ctggagggca tgttctgcac cgtcatccgc accgtcatcg agcgcgtcgg cgacttggtt gccatgctga accccagcca gctcatcgac cccaacggaa acgtgcgcga ccccgccaac tacaagttca cgccgaaacg ccctttccgc cgcctccgct acgctgacgc gatccagttc tgcaacgaga acggcatctt gaacaccgag acaggcaagc ctttcgagtt cggggaggac atcactgatc agccggaacg cgcgatggtg gccaagctgg gcgagtttgt gtttatgacg cacttccctg ccagcatgaa gtctttttac atgcagcgtg accctgatga cccgacgctg acggagtcgg tcgacgtcct ggcgcccggc attggcgagg tgcttggtgg ctcgatgcgt atgtacaagt acgacgagct gctcgacgcg tacaagcgcg agggtctgga tgcgtccacg tactactggt acacggacca gcgccgctac ggcggtgccc cgcacggcgg ctttggcctc ggtgttgagc gtctgctggt gtggatgctg aacctcgata gcgtgaagga cgcatgcctg tacccccgtt acatgggccg ctgcaagccg tag

Details for LedoB.00825.b.B2.GE42672

HARVESTED ON:	9/28/2018
SEQUENCED ON:	10/17/2018
EXPECTED MW:	96kDa
OBSERVED MW:	97kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHEK EAPGWQSTIQ VNLAMITEKP HALASPMMAS SVRPAVDEAP SFATTSPPLA PGRGFTELSC SVLVPVSLEP PLPATSLPWR DRGLRTGTCA SSSSALVLTA ASSLPSSLRQ HWSSCTTLSR CPFFSLALPL CLLRHRRTLP PHHHHRSPRL PLHTPCRTIA IQLHSVLLFK ASGSKGPRAE SERTMENEQQ RTAAVAQLKA VVGLDDRGAK DLSSKPERVA DVLAFFAQHS IDEASPREQK VMLFNVWTKV KKPEHRDPVT AFILDGKLDS TQKVDAAIRF VNAAGSDVVD TAAMLAECGV GVTVSREDVE KAVAAALVAE DVAALKTKWD KNPNMMLGQM RRVAALRWAD VEHVRAALER QVPGLIKDVI VEAKPAAAKK EAAAMPKKEV ESCRQNSNFK DVAQGLPRSP IGELPSHKEG ACVYVVGWAH RVRHQSRMSF VVLRDGTGFI QCVFDGSTEP FHRESCVAIR GTLRHEPKAK SELQPPMELH VNEYAVVGDS DGTIETVITA ESSVDKLYDQ RHVVVRGTLA SSVLKVRHEL LRVFREHFWS HQYYEVTPPT LVQTQVEGGS TLFEVLYYGE KAYLTQSSQL YLESVTASLG NVYCCMPSYR AERSKTKRHL SEFTHLEAEY DVCSFEDLLN HLEGMFCTVI RTVIERVGDL VAMLNPSQLI DPNGNVRDPA NYKFTPKRPF RRLRYADAIQ FCNENGILNT ETGKPFEFGE DITDQPERAM VAKLGEFVFM THFPASMKSF YMQRDPDDPT LTESVDVLAP GIGEVLGGSM RMYKYDELLD AYKREGLDAS TYYWYTDQRR YGGAPHGGFG LGVERLLVWM LNLDSVKDAC LYPRYMGRCK P

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaaaagg aggcgccggg ctggcagtcg acaatacagg tgaatctggc gatgatcacg gaaaagccgc atgcgcttgc ttccccgatg atggcaagta gtgtccggcc ggcggtggac gaggcgccct cttttgcaac gacgtcaccg cctcttgcac ccggtcgcgg gttcacagag ctcagttgct cagttctggt acccgtctct ttagagccac cactaccggc gacctccttg ccgtggcgtg atcgaggttt gcgcacgggc acctgcgcat cttcctcatc agcgctagtt ctgacggccg cctcgtctct tccgtcttct cttcgtcaac actggtcctc ttgcactact ctctcccgct gccctttctt ctctctggcg ttgccgctct gcctgctgcg tcaccgtcgg accctgcccc ctcaccatca ccaccgatca ccgcgtctgc cgctgcacac gccctgcaga acaatagcta ttcagctaca ctccgtgtta ctctttaagg catcgggctc gaaaggccca cgggcggaat cagaacgcac catggagaac gagcagcagc gcaccgctgc cgttgcgcag ctcaaagctg ttgttggcct tgacgacagg ggcgccaaag acctgtccag caagccggaa cgcgttgccg atgtgctggc cttcttcgcg cagcacagca tcgatgaagc ttctcctcgt gagcagaagg tcatgctgtt caacgtgtgg acaaaggtga agaagcctga gcaccgcgat cccgtgaccg ccttcatcct tgacggcaaa ctcgacagca cgcagaaggt cgacgccgcc atcaggtttg taaacgcggc cgggagtgat gtggtggaca cggcggccat gctggcggag tgcggcgtcg gggtgacggt gtcgcgggag gacgtggaga aggcagttgc cgcggccctc gtcgctgaag acgtggccgc gctcaagacg aagtgggaca agaacccgaa catgatgctt ggccagatgc gccgtgtggc ggccctgcgc tgggccgacg tggagcacgt gcgcgcggcg ctggagaggc aggttccggg gttgatcaag gacgttatcg tggaggcgaa gccggcagcc gcgaagaagg aagccgctgc tatgccgaag aaggaggtag agtcgtgccg gcagaactcg aacttcaagg atgttgcgca ggggctaccg cgctccccaa ttggcgagct gccgtcgcac aaagagggcg cctgcgtcta cgtcgttggt tgggcccacc gcgtgcgcca ccagtctcgc atgtcctttg tggtgctgcg cgatggcacg ggcttcatcc agtgcgtctt tgacggctcc acggagccct tccaccgcga atcgtgcgtc gccattcgcg ggaccctgcg tcacgagccg aaagcgaagt cggagctgca gccgccgatg gagctgcacg tgaacgagta cgctgtcgtt ggtgactccg acggtacgat tgagacggtc atcacggctg agagctccgt tgacaaactg tacgaccagc gccacgttgt ggtgcgcggc acgctggcgt cgtctgtgct aaaggtccgg cacgagctgt tgcgcgtgtt ccgcgagcac ttctggtcgc accagtacta cgaggtgacg ccgccgacgc tggtgcagac gcaggtggag ggcggctcga ccctgttcga ggtcctgtac tacggcgaga aggcgtactt gacgcagtcg tcgcagcttt accttgaaag cgtgacggcg tcgctgggca acgtgtactg ctgcatgccg agctaccgcg cggagaggtc caagacgaag cgccacctca gcgagttcac gcacctggag gccgagtacg acgtgtgctc ctttgaagat cttttgaatc acctggaggg catgttctgc accgtcatcc gcaccgtcat cgagcgcgtc ggcgacttgg ttgccatgct gaaccccagc cagctcatcg accccaacgg aaacgtgcgc gaccccgcca actacaagtt cacgccgaaa cgccctttcc gccgcctccg ctacgctgac gcgatccagt tctgcaacga gaacggcatc ttgaacaccg agacaggcaa gcctttcgag ttcggggagg acatcactga tcagccggaa cgcgcgatgg tggccaagct gggcgagttt gtgtttatga cgcacttccc tgccagcatg aagtcttttt acatgcagcg tgaccctgat gacccgacgc tgacggagtc ggtcgacgtc ctggcgcccg gcattggcga ggtgcttggt ggctcgatgc gtatgtacaa gtacgacgag ctgctcgacg cgtacaagcg cgagggtctg gatgcgtcca cgtactactg gtacacggac cagcgccgct acggcggtgc cccgcacggc ggctttggcc tcggtgttga gcgtctgctg gtgtggatgc tgaacctcga tagcgtgaag gacgcatgcc tgtacccccg ttacatgggc cgctgcaagc cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat