LedoB.00612.a

lysyl-tRNA synthetase, putative

CENTER ID: LedoB.00612.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.00612.a.B1.GE42601 full length 1 536
LedoB.00612.a.B2.GE43048 XtalPred domain #1 59 498
LedoB.00612.a.B3.GE43049 XtalPred domain #2 59 383
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LdBPK_300130.1
UniProt: E9BLA8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGGILFITIR SGEAQLQVLR QVNESFTKAD LRAFASTLHT GDILGATGVP GRTAKGELSL YATEASILAP YVCTDQALCP DLKGFVPLTD TGIKYRYRFV DMMSSPSVVQ HFRKRHAITR ALRDFLDARS FVEVETPVLH SVASGANAKP FVTHHNANDA DLFLRVAPEL HLKQCVVGGM ERVYEIGRVF RNEDADRSHN PEFTTCEFYA AYHTYKDLMT MTEEILRHLA LAANGTTKIT VTSCVEKKPV EIDLAQPFRR VNAYDAVQEA AGVELPPPTE LSTPRGLAYM SAIMLRYNIP LPSVRTAAKM FDKLIDYFIT DRVVEPTFVT DHPLFMSPLA MEQRTRPGLS ARFELFINGT EYCNAYSELN DPQEQYYRFQ QQLLDRQTGD DEAMALDETF LKALQVGLPP TAGWGMGIDR VAMLLNGSST IREDILFPLL RHDATSHDAK RRCKTASFFG FNHNMTLFCL NALEEEMLKR AAPTESLEKI RQLRQCLSTM QQRDMVTYAP EDGAPRGWRY EATMAVLRLF CYRGKY
NT Sequence
atgggtggta ttcttttcat caccatccgc agcggcgaag cccaactgca ggtgctgcgg caggtgaacg agtccttcac caaggctgac ctgcgtgcct ttgctagtac actgcacacg ggcgacatcc tcggtgccac cggcgtgccg ggtcgcaccg cgaaaggaga gctctccctc tacgcgacgg aggcctccat cttggcaccg tacgtctgca cagaccaggc gctgtgcccc gacctgaagg gcttcgtgcc gctcacggac accggcatca aataccgcta ccgctttgtc gacatgatgt ccagcccgtc cgtcgtgcag catttccgca agcgccacgc catcacgcgc gctctgcgcg actttctcga cgcgcgcagc ttcgtcgagg tggagacgcc ggtgctgcac tccgtcgcct caggtgccaa cgcgaagccg tttgtcacgc accacaacgc caacgacgcg gatctcttcc ttcgggtggc cccagagctt cacctgaaac agtgcgttgt gggcggcatg gagcgcgtgt acgagatcgg ccgcgtcttc cggaacgagg acgccgaccg cagccacaac cccgaattca ccacgtgcga gttctacgcc gcctatcaca cctacaagga tctgatgacc atgacggaag agatcctgcg ccacttggcg ctcgcggcga acggcaccac caaaatcact gtgacgtcgt gcgtggaaaa gaagccggtg gaaatcgacc ttgcgcagcc gtttcgccgc gtcaacgcgt acgacgcagt gcaggaggcg gccggcgtcg agctgccgcc cccgacagag ctgagtacgc cgcgtggact ggcatacatg tctgccatca tgctccgcta caacatcccg ctgccgtcgg tgcgcacggc tgccaagatg ttcgacaagc tcatcgacta cttcatcact gatcgcgtgg tggagccgac gttcgtgacg gaccatccgc tgttcatgag cccgctggcg atggagcagc gcacgcggcc ggggctgtcg gctcgtttcg agctattcat caatggcacc gagtactgca acgcgtacag cgagctgaat gacccgcagg agcagtacta ccgctttcag cagcagctgc tggacaggca aaccggcgac gacgaggcga tggcgctgga cgaaacgttt ctcaaggcgc tgcaggtcgg gctgccccca acggcggggt gggggatggg gatcgaccgc gtcgccatgc tcctcaacgg gtcctccacc atccgcgagg acattttgtt tccgctgctc cgccacgacg ccacgtcgca cgatgcaaaa cgtcggtgta agacggcctc ttttttcggc ttcaaccaca acatgacgct cttctgtctc aacgcgctcg aagaggagat gctgaagcgc gccgctccga cggagtcgct cgagaagatt cgccagctgc gccaatgcct gtccacgatg cagcagcgcg acatggtgac gtacgcgccc gaggatggcg caccgcgagg gtggcgctat gaggcaacca tggccgtgct ccgccttttc tgctaccgcg gaaagtactg a
Details for LedoB.00612.a.B1.GE42601
HARVESTED ON: data unavailable
SEQUENCED ON: 8/8/2018
EXPECTED MW: 61kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 74
PERCENT COVERAGE: 60
Validated AA Sequence
MAHHHHHHMG GILFITIRSG EAQLQVLRQV NESFTKADLR AFASTLHTGD ILGATGVPGR TAKGELSLYA TEASILAPYV CTDQALCPDL KGFVPLTDTG IKYRYRFVDM MSSPSVVXHF XKRHAITRAL RDFLDARSFV EVETPVLHSV ASXANAKPFV THHNANDADL FLRVAPXLHL KQCVVGGMER VYEIGRVFRN EDADRSHNPE FTTCXXYAAY HTYKDLMTXX XXILRHLAXA ANXXXQNHXD VXRGXXPGGX XXXXXXXXXQ RXXXXXXGGR RRAAAPDXXE XXXXTGIHVC HHAPXQHPAA VGAHGCQDVX QAHRXXHH
Validated NT Sequence
atggctcacc accaccacca ccatatgggt ggtattcttt tcatcaccat ccgcagcggc gaagcccaac tgcaggtgct gcggcaggtg aacgagtcct tcaccaaggc tgacctgcgt gcctttgcta gtacactgca cacgggcgac atcctcggtg ccaccggcgt gccgggtcgc accgcgaaag gagagctctc cctctacgcg acggaggcct ccatcttggc accgtacgtc tgcacagacc aggcgctgtg ccccgacctg aagggcttcg tgccgctcac ggacaccggc atcaaatacc gctaccgctt tgtcgacatg atgtccagcc cgtccgtcgt gcnccatttc nnnaagcgcc acgccatcac gcgcgctctg cgcgactttc tcgacgcgcg cagcttcgtc gaggtggaga cgccggtgct gcactccgtc gcctcanntg ccaacgcgaa gccgtttgtc acgcaccaca acgccaacga cgcggatctc ttccttcggg tggccccnnn gcttcacctg aaacagtgcg ttgtgggcgg catggagcgc gtgtacgaga tcggccgcgt cttccggaac gaggacgccg accgcagcca caaccccgaa ttcaccacgt gcgnnntcta cgccgcctat cacacctaca aggatctgat gaccatnnnn nnnnnnatcc tgcgccactt ggcnntcgcg gcgaacgnnn nnnnncaaaa tcacngtgac gtnnngcgtg gnnnnnaacc cggtggaaan nnnncnnnnn nanncnnntn nnnnnntcaa cgnnnnnnnn nnnnnnnnng aggcggccgg cgtcgagctg ccgcccccga cagnnnngag tannncnnnn nnactggcat acatgtctgc catcatgctc cnnnacaaca tcccgctgcc gtcggtgcgc acggctgcca agatgtnnna caagctcatc gnnnanttca tcactgannn cgtggtggag ccnacgttcg tgannnnnca tccgctgttc atgagcccgc nnncnnngga gcagcgcacg cggccggggc tgtcggctcg tttcgagcta ttcatcaatg gcaccgagta ctgcaacgcg tacagcgagc tgaatgaccc gcaggagcag tactaccgct ttcagcagca gctgctggac aggcaaaccg gcgacgacga ggcgatggcg ctggacgaaa cgtttctcaa ggcgctgcag gtcgggctgc ccccaacggc ggggtggggg atggggatcg accgcgtcgc catgctcctc aacgggtcct ccaccatccg cgaggacatt ttgtttccgc tgctccgcca cgacgccacg tcgcacgatg caaaacgtcg gtgtaagacg gcctcttttt tcggcttcaa ccacaacatg acgcnnntct gtctcaacgc gcnnnaagag gagatgctga agcgcgccgc tccgacggag tcgctcnaga agattcgcca gctgcgccaa tgcctgtcca cgatgcagca gcgcgacatg gtgacgtacg cgcccgagga tggcgnnncg cgagggtggc gctatgaggc aaccatggcc gtgctc
Expected Protein Sequence
MAHHHHHHMG GILFITIRSG EAQLQVLRQV NESFTKADLR AFASTLHTGD ILGATGVPGR TAKGELSLYA TEASILAPYV CTDQALCPDL KGFVPLTDTG IKYRYRFVDM MSSPSVVQHF RKRHAITRAL RDFLDARSFV EVETPVLHSV ASGANAKPFV THHNANDADL FLRVAPELHL KQCVVGGMER VYEIGRVFRN EDADRSHNPE FTTCEFYAAY HTYKDLMTMT EEILRHLALA ANGTTKITVT SCVEKKPVEI DLAQPFRRVN AYDAVQEAAG VELPPPTELS TPRGLAYMSA IMLRYNIPLP SVRTAAKMFD KLIDYFITDR VVEPTFVTDH PLFMSPLAME QRTRPGLSAR FELFINGTEY CNAYSELNDP QEQYYRFQQQ LLDRQTGDDE AMALDETFLK ALQVGLPPTA GWGMGIDRVA MLLNGSSTIR EDILFPLLRH DATSHDAKRR CKTASFFGFN HNMTLFCLNA LEEEMLKRAA PTESLEKIRQ LRQCLSTMQQ RDMVTYAPED GAPRGWRYEA TMAVLRLFCY RGKY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggtg gtattctttt catcaccatc cgcagcggcg aagcccaact gcaggtgctg cggcaggtga acgagtcctt caccaaggct gacctgcgtg cctttgctag tacactgcac acgggcgaca tcctcggtgc caccggcgtg ccgggtcgca ccgcgaaagg agagctctcc ctctacgcga cggaggcctc catcttggca ccgtacgtct gcacagacca ggcgctgtgc cccgacctga agggcttcgt gccgctcacg gacaccggca tcaaataccg ctaccgcttt gtcgacatga tgtccagccc gtccgtcgtg cagcatttcc gcaagcgcca cgccatcacg cgcgctctgc gcgactttct cgacgcgcgc agcttcgtcg aggtggagac gccggtgctg cactccgtcg cctcaggtgc caacgcgaag ccgtttgtca cgcaccacaa cgccaacgac gcggatctct tccttcgggt ggccccagag cttcacctga aacagtgcgt tgtgggcggc atggagcgcg tgtacgagat cggccgcgtc ttccggaacg aggacgccga ccgcagccac aaccccgaat tcaccacgtg cgagttctac gccgcctatc acacctacaa ggatctgatg accatgacgg aagagatcct gcgccacttg gcgctcgcgg cgaacggcac caccaaaatc actgtgacgt cgtgcgtgga aaagaagccg gtggaaatcg accttgcgca gccgtttcgc cgcgtcaacg cgtacgacgc agtgcaggag gcggccggcg tcgagctgcc gcccccgaca gagctgagta cgccgcgtgg actggcatac atgtctgcca tcatgctccg ctacaacatc ccgctgccgt cggtgcgcac ggctgccaag atgttcgaca agctcatcga ctacttcatc actgatcgcg tggtggagcc gacgttcgtg acggaccatc cgctgttcat gagcccgctg gcgatggagc agcgcacgcg gccggggctg tcggctcgtt tcgagctatt catcaatggc accgagtact gcaacgcgta cagcgagctg aatgacccgc aggagcagta ctaccgcttt cagcagcagc tgctggacag gcaaaccggc gacgacgagg cgatggcgct ggacgaaacg tttctcaagg cgctgcaggt cgggctgccc ccaacggcgg ggtgggggat ggggatcgac cgcgtcgcca tgctcctcaa cgggtcctcc accatccgcg aggacatttt gtttccgctg ctccgccacg acgccacgtc gcacgatgca aaacgtcggt gtaagacggc ctcttttttc ggcttcaacc acaacatgac gctcttctgt ctcaacgcgc tcgaagagga gatgctgaag cgcgccgctc cgacggagtc gctcgagaag attcgccagc tgcgccaatg cctgtccacg atgcagcagc gcgacatggt gacgtacgcg cccgaggatg gcgcaccgcg agggtggcgc tatgaggcaa ccatggccgt gctccgcctt ttctgctacc gcggaaagta ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for LedoB.00612.a.B2.GE43048
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHSL YATEASILAP YVCTDQALCP DLKGFVPLTD TGIKYRYRFV DMMSSPSVVQ HFRKRHAITR ALRDFLDARS FVEVETPVLH SVASGANAKP FVTHHNANDA DLFLRVAPEL HLKQCVVGGM ERVYEIGRVF RNEDADRSHN PEFTTCEFYA AYHTYKDLMT MTEEILRHLA LAANGTTKIT VTSCVEKKPV EIDLAQPFRR VNAYDAVQEA AGVELPPPTE LSTPRGLAYM SAIMLRYNIP LPSVRTAAKM FDKLIDYFIT DRVVEPTFVT DHPLFMSPLA MEQRTRPGLS ARFELFINGT EYCNAYSELN DPQEQYYRFQ QQLLDRQTGD DEAMALDETF LKALQVGLPP TAGWGMGIDR VAMLLNGSST IREDILFPLL RHDATSHDAK RRCKTASFFG FNHNMTLFCL NALEEEMLKR AAPTESLEKI RQLRQCLS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccctct acgcgacgga ggcctccatc ttggcaccgt acgtctgcac agaccaggcg ctgtgccccg acctgaaggg cttcgtgccg ctcacggaca ccggcatcaa ataccgctac cgctttgtcg acatgatgtc cagcccgtcc gtcgtgcagc atttccgcaa gcgccacgcc atcacgcgcg ctctgcgcga ctttctcgac gcgcgcagct tcgtcgaggt ggagacgccg gtgctgcact ccgtcgcctc aggtgccaac gcgaagccgt ttgtcacgca ccacaacgcc aacgacgcgg atctcttcct tcgggtggcc ccagagcttc acctgaaaca gtgcgttgtg ggcggcatgg agcgcgtgta cgagatcggc cgcgtcttcc ggaacgagga cgccgaccgc agccacaacc ccgaattcac cacgtgcgag ttctacgccg cctatcacac ctacaaggat ctgatgacca tgacggaaga gatcctgcgc cacttggcgc tcgcggcgaa cggcaccacc aaaatcactg tgacgtcgtg cgtggaaaag aagccggtgg aaatcgacct tgcgcagccg tttcgccgcg tcaacgcgta cgacgcagtg caggaggcgg ccggcgtcga gctgccgccc ccgacagagc tgagtacgcc gcgtggactg gcatacatgt ctgccatcat gctccgctac aacatcccgc tgccgtcggt gcgcacggct gccaagatgt tcgacaagct catcgactac ttcatcactg atcgcgtggt ggagccgacg ttcgtgacgg accatccgct gttcatgagc ccgctggcga tggagcagcg cacgcggccg gggctgtcgg ctcgtttcga gctattcatc aatggcaccg agtactgcaa cgcgtacagc gagctgaatg acccgcagga gcagtactac cgctttcagc agcagctgct ggacaggcaa accggcgacg acgaggcgat ggcgctggac gaaacgtttc tcaaggcgct gcaggtcggg ctgcccccaa cggcggggtg ggggatgggg atcgaccgcg tcgccatgct cctcaacggg tcctccacca tccgcgagga cattttgttt ccgctgctcc gccacgacgc cacgtcgcac gatgcaaaac gtcggtgtaa gacggcctct tttttcggct tcaaccacaa catgacgctc ttctgtctca acgcgctcga agaggagatg ctgaagcgcg ccgctccgac ggagtcgctc gagaagattc gccagctgcg ccaatgcctg tcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for LedoB.00612.a.B3.GE43049
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/25/2019
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS LYATEASILA PYVCTDQALC PDLKGFVPLT DTGIKYRYRF VDMMSSPSVV QHFRKRHAIT RALRDFLDAR SFVEVETPVL HSVASGANAK PFVTHHNAND ADLFLRVAPE LHLKQCVVGG MERVYEIGRV FRNEDADRSH NPEFTTCEFY AAYHTYKDLM TMTEEILRHL ALAANGTTKI TVTSCVEKKP VEIDLAQPFR RVNAYDAVQE AAGVELPPPT ELSTPRGLAY MSAIMLRYNI PLPSVRTAAK MFDKLIDYFI TDRVVEPTFV TDHPLFMSPL AMEQRTRPGL SARFELFING TEYCNAYSEL NDPQ
Validated NT Sequence
cctgcgggtc attcagctcg ctgtacgcgt tgcagtactc ggtgccattg atgaatagct cgaaacgagc cgacagcccc ggccgcgtgc gctgctccat cgccagcggg ctcatgaaca gcggatggtc cgtcacgaac gtcggctcca ccacgcgatc agtgatgaag tagtcgatga gcttgtcgaa catcttggca gccgtgcgca ccgacggcag cgggatgttg tagcggagca tgatggcaga catgtatgcc agtccacgcg gcgtactcag ctctgtcggg ggcggcagct cgacgccggc cgcctcctgc actgcgtcgt acgcgttgac gcggcgaaac ggctgcgcaa ggtcgatttc caccggcttc ttttccacgc acgacgtcac agtgattttg gtggtgccgt tcgccgcgag cgccaagtgg cgcaggatct cttccgtcat ggtcatcaga tccttgtagg tgtgataggc ggcgtagaac tcgcacgtgg tgaattcggg gttgtggctg cggtcggcgt cctcgttccg gaagacgcgg ccgatctcgt acacgcgctc catgccgccc acaacgcact gtttcaggtg aagctctggg gccacccgaa ggaagagatc cgcgtcgttg gcgttgtggt gcgtgacaaa cggcttcgcg ttggcacctg aggcgacgga gtgcagcacc ggcgtctcca cctcgacgaa gctgcgcgcg tcgagaaagt cgcgcagagc gcgcgtgatg gcgtggcgct tgcggaaatg ctgcacgacg gacgggctgg acatcatgtc gacaaagcgg tagcggtatt tgatgccggt gtccgtgagc ggcacgaagc ccttcaggtc ggggcacagc gcctggtctg tgcagacgta cggtgccaag atggaggcct ccgtcgcgta gagggacata tggtggtggt ggtggtgagc catggtatat c
Expected Protein Sequence
MAHHHHHHSL YATEASILAP YVCTDQALCP DLKGFVPLTD TGIKYRYRFV DMMSSPSVVQ HFRKRHAITR ALRDFLDARS FVEVETPVLH SVASGANAKP FVTHHNANDA DLFLRVAPEL HLKQCVVGGM ERVYEIGRVF RNEDADRSHN PEFTTCEFYA AYHTYKDLMT MTEEILRHLA LAANGTTKIT VTSCVEKKPV EIDLAQPFRR VNAYDAVQEA AGVELPPPTE LSTPRGLAYM SAIMLRYNIP LPSVRTAAKM FDKLIDYFIT DRVVEPTFVT DHPLFMSPLA MEQRTRPGLS ARFELFINGT EYCNAYSELN DPQEQYYRFQ QQL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccctct acgcgacgga ggcctccatc ttggcaccgt acgtctgcac agaccaggcg ctgtgccccg acctgaaggg cttcgtgccg ctcacggaca ccggcatcaa ataccgctac cgctttgtcg acatgatgtc cagcccgtcc gtcgtgcagc atttccgcaa gcgccacgcc atcacgcgcg ctctgcgcga ctttctcgac gcgcgcagct tcgtcgaggt ggagacgccg gtgctgcact ccgtcgcctc aggtgccaac gcgaagccgt ttgtcacgca ccacaacgcc aacgacgcgg atctcttcct tcgggtggcc ccagagcttc acctgaaaca gtgcgttgtg ggcggcatgg agcgcgtgta cgagatcggc cgcgtcttcc ggaacgagga cgccgaccgc agccacaacc ccgaattcac cacgtgcgag ttctacgccg cctatcacac ctacaaggat ctgatgacca tgacggaaga gatcctgcgc cacttggcgc tcgcggcgaa cggcaccacc aaaatcactg tgacgtcgtg cgtggaaaag aagccggtgg aaatcgacct tgcgcagccg tttcgccgcg tcaacgcgta cgacgcagtg caggaggcgg ccggcgtcga gctgccgccc ccgacagagc tgagtacgcc gcgtggactg gcatacatgt ctgccatcat gctccgctac aacatcccgc tgccgtcggt gcgcacggct gccaagatgt tcgacaagct catcgactac ttcatcactg atcgcgtggt ggagccgacg ttcgtgacgg accatccgct gttcatgagc ccgctggcga tggagcagcg cacgcggccg gggctgtcgg ctcgtttcga gctattcatc aatggcaccg agtactgcaa cgcgtacagc gagctgaatg acccgcagga gcagtactac cgctttcagc agcagctgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat