LedoB.00133.a

cysteinyl-tRNA synthetase, putative

CENTER ID: LedoB.00133.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.00133.a.B1.GE42600 full length 1 784
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LdBPK_120255.1
UniProt: E9BB00

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSANRVPTVV AGLDGANPVK RERHPAWYPP LKLDGNGLKV MNSLTETLED FAPRDGRVVR WYTCGPTVYD LSHMGHARAY LTFDIIRRVM EDYFGYSVIY QMNITDIDDK IIKRARVNKL LDDFKEGELR HGDVARLMAF TAEAVTAAES GLAKRKAALF EPITEGASSR AKADREEKLL EVQLKESQLV ETKVKIAAAK DDFAALFAAA SGVNGDLLDE RSGHSVSDQQ IFEDHARKYE RAFFEDMQRL GIREPDIVTR VTEYVPQVVE FVQKIIDNGF AYVGETSVFF DTEAYIRAGH DYPKLKPGGD RNTTEDEMAE GEGVLSKGIE GEKRSPNDFA LWKFSKPGEP RWPSPWGEGR PGWHIECSVM ASDILGENMD IHSGGWDLKF PHHDNECAQS EACNLHSQWV NYFLHCGHLH IKGLKMSKSL KNFITIRQAL DELGVTARTM RLLFLANPWN KPMNFSDQSL DEAKEKERVL RAFFGSVDIV LRTDNWKATQ GANKHDRELL SKWVEAEAAV HAALQDNFDT VVALQQLMSL VAATNQYLLS GERPSATLVR KVGCYVTKMF RIFGVVEGSD DVGLQKQGSG DGEARFIEVV NTLVRFRDEV RDAAKDHKVV AGFLPLCDKV RDEWLVDAGV RLEDNPAGPT TWKSDEPALL HKELAERRAQ QEGDRQRRLA NQAETKRKLV EKWRQFTYPP SEYFRLQDEQ RVEKKYAAYD DVTGLPTMTA AGEEVSEKEA KKLSKEQAKY AKSYDEFIGK GGVSWLQEQE AELASMVAEL KGSN
NT Sequence
atgagcgcca atcgtgttcc gacggtagta gccggcctcg atggagccaa cccggtgaag cgagagcgcc accccgcgtg gtacccgccc ctgaagctgg acggcaacgg gctgaaggtg atgaactccc tgacagagac ccttgaggac ttcgcgccgc gtgatggccg cgtggtgcga tggtacacgt gcggcccgac cgtctacgat ctctctcaca tgggccacgc ccgtgcttac ctcaccttcg acatcatccg ccgtgtcatg gaagactact tcggctactc cgtgatttat cagatgaaca tcacggatat cgacgacaag atcatcaagc gcgctcgcgt gaataagctg ctggatgact tcaaggaggg ggagctgcgc cacggcgacg tggcgaggct catggcgttc acggcggagg ccgtcaccgc cgccgagagc ggccttgcca agcgcaaggc ggcgctgttc gagccgatta ccgaaggcgc cagcagccgc gccaaggcgg accgcgagga gaagttgcta gaggtgcagc tgaaggagtc gcagcttgtg gagaccaagg tgaagatcgc ggcggccaag gatgactttg ccgccctgtt cgccgcggcc agcggcgtga acggcgacct gcttgacgag cgcagcggcc acagcgtctc cgatcagcag atcttcgaag accacgcccg aaaatacgag cgagcgttct tcgaggacat gcagcgcttg ggcattcgtg agccggacat cgtgacgcgc gtcacggagt acgtgccgca ggtggtggag ttcgtgcaga aaatcatcga caacggcttt gcctacgtcg gcgagacatc tgtcttcttt gacaccgagg catacatcag ggccggtcac gactacccga agctgaagcc tggcggcgat cgcaacacca cggaggacga gatggccgag ggcgagggcg tactttcgaa aggcatcgag ggcgagaagc gcagcccgaa cgactttgct ctgtggaagt tctcgaagcc cggcgagccg cgctggccat ccccgtgggg cgagggtcgt ccgggctggc acatcgagtg ctccgtgatg gcgtccgaca ttctcggcga gaacatggac atccacagcg gtgggtggga cttgaagttt ccgcaccacg acaacgagtg cgcgcagagc gaggcgtgca acctgcacag ccagtgggtg aactacttcc tccactgcgg ccatctccac atcaaggggc tgaagatgag caagagcctc aagaacttca tcaccatccg ccaggcgctg gacgaactcg gcgtcacggc gcgcacgatg cggctgctgt tccttgcaaa tccctggaac aagccgatga acttctcgga ccagtcgctc gacgaggcga aggaaaagga gcgggtcctg cgcgccttct tcggcagcgt cgacattgtc ctccgcaccg ataactggaa ggccacgcag ggtgccaaca agcacgaccg cgagctgctg tccaagtggg tcgaggccga ggcagccgtg cacgcggcgt tgcaggacaa ctttgacacg gtcgttgccc tgcagcagct catgtctctc gtcgcggcga cgaaccagta cctgctgtct ggcgagcggc cgagtgcgac gctggtgcgc aaggtaggct gctacgtgac gaagatgttc cgcatcttcg gcgtcgtcga ggggtctgac gatgtcggcc tgcagaagca gggctccggc gatggcgaag cgcgcttcat cgaggtcgtg aacactctcg tgcgcttccg tgatgaggtg cgcgatgcgg cgaaagacca caaggtggtg gcgggcttcc tgcccttgtg cgacaaggtg cgcgatgagt ggcttgtcga cgctggcgta cggctcgagg acaacccggc gggcccgacg acgtggaaga gcgacgagcc ggcgctgctg cacaaagagc tggcggagcg ccgcgctcag caagaagggg atcgccagag gaggctggcg aatcaggcgg agacgaagcg caagctcgtg gaaaagtggc ggcagttcac ctacccgccg tccgagtact ttcgcctcca ggacgagcaa cgggtagaga agaagtacgc tgcctacgac gacgtcaccg gcctcccaac gatgacggcg gccggcgagg aggtgagcga gaaggaggca aagaagttga gcaaggagca ggcgaagtac gccaagtcgt acgacgagtt tatcggcaag ggtggcgtgt cgtggcttca agagcaggag gcggagctgg ccagcatggt ggccgagctc aaaggcagca attag
Details for LedoB.00133.a.B1.GE42600
HARVESTED ON: data unavailable
SEQUENCED ON: 8/8/2018
EXPECTED MW: 89kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS ANRVPTVVAG LDGANPVKRE RHPAWYPPLK LDGNGLKVMN SLTETLEDFA PRDGRVVRWY TCGPTVYDLS HMGHARAYLT FDIIRRVMED YFGYSVIYQM NITDIDDKII KRARVNKLLD DFKEGELRHG DVARLMAFTA EAVTAAESGL AKRKAALFEP ITEGASSRAK ADREEKLLEV QLKESQLVET KVKIAAAKDD FAALFAAASG VNGDLLDERS GHSVSDQQIF EDHARKYERA FFEDMQRLGI REPDIVTRVT EYVPQVVEFV QKIIDNGFAY VGETSVFFDT EAYIRAGHDY PKLKPGGDRN TTEDEMAEGE GVLSKGIEGE KRSPNDFALW KFSKPGEPRW PSPWGEGRPG WHIECSVMAS DILGENMDIH SGGWDLKFPH HDNECAQSEA CNLHSQWVNY FLHCGHLHIK GLKMSKSLKN FITIRQALDE LGVTARTMRL LFLANPWNKP MNFSDQSLDE AKEKERVLRA FFGSVDIVLR TDNWKATQGA NKHDRELLSK WVEAEAAVHA ALQDNFDTVV ALQQLMSLVA ATNQYLLSGE RPSATLVRKV GCYVTKMFRI FGVVEGSDDV GLQKQGSGDG EARFIEVVNT LVRFRDEVRD AAKDHKVVAG FLPLCDKVRD EWLVDAGVRL EDNPAGPTTW KSDEPALLHK ELAERRAQQE GDRQRRLANQ AETKRKLVEK WRQFTYPPSE YFRLQDEQRV EKKYAAYDDV TGLPTMTAAG EEVSEKEAKK LSKEQAKYAK SYDEFIGKGG VSWLQEQEAE LASMVAELKG SN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg ccaatcgtgt tccgacggta gtagccggcc tcgatggagc caacccggtg aagcgagagc gccaccccgc gtggtacccg cccctgaagc tggacggcaa cgggctgaag gtgatgaact ccctgacaga gacccttgag gacttcgcgc cgcgtgatgg ccgcgtggtg cgatggtaca cgtgcggccc gaccgtctac gatctctctc acatgggcca cgcccgtgct tacctcacct tcgacatcat ccgccgtgtc atggaagact acttcggcta ctccgtgatt tatcagatga acatcacgga tatcgacgac aagatcatca agcgcgctcg cgtgaataag ctgctggatg acttcaagga gggggagctg cgccacggcg acgtggcgag gctcatggcg ttcacggcgg aggccgtcac cgccgccgag agcggccttg ccaagcgcaa ggcggcgctg ttcgagccga ttaccgaagg cgccagcagc cgcgccaagg cggaccgcga ggagaagttg ctagaggtgc agctgaagga gtcgcagctt gtggagacca aggtgaagat cgcggcggcc aaggatgact ttgccgccct gttcgccgcg gccagcggcg tgaacggcga cctgcttgac gagcgcagcg gccacagcgt ctccgatcag cagatcttcg aagaccacgc ccgaaaatac gagcgagcgt tcttcgagga catgcagcgc ttgggcattc gtgagccgga catcgtgacg cgcgtcacgg agtacgtgcc gcaggtggtg gagttcgtgc agaaaatcat cgacaacggc tttgcctacg tcggcgagac atctgtcttc tttgacaccg aggcatacat cagggccggt cacgactacc cgaagctgaa gcctggcggc gatcgcaaca ccacggagga cgagatggcc gagggcgagg gcgtactttc gaaaggcatc gagggcgaga agcgcagccc gaacgacttt gctctgtgga agttctcgaa gcccggcgag ccgcgctggc catccccgtg gggcgagggt cgtccgggct ggcacatcga gtgctccgtg atggcgtccg acattctcgg cgagaacatg gacatccaca gcggtgggtg ggacttgaag tttccgcacc acgacaacga gtgcgcgcag agcgaggcgt gcaacctgca cagccagtgg gtgaactact tcctccactg cggccatctc cacatcaagg ggctgaagat gagcaagagc ctcaagaact tcatcaccat ccgccaggcg ctggacgaac tcggcgtcac ggcgcgcacg atgcggctgc tgttccttgc aaatccctgg aacaagccga tgaacttctc ggaccagtcg ctcgacgagg cgaaggaaaa ggagcgggtc ctgcgcgcct tcttcggcag cgtcgacatt gtcctccgca ccgataactg gaaggccacg cagggtgcca acaagcacga ccgcgagctg ctgtccaagt gggtcgaggc cgaggcagcc gtgcacgcgg cgttgcagga caactttgac acggtcgttg ccctgcagca gctcatgtct ctcgtcgcgg cgacgaacca gtacctgctg tctggcgagc ggccgagtgc gacgctggtg cgcaaggtag gctgctacgt gacgaagatg ttccgcatct tcggcgtcgt cgaggggtct gacgatgtcg gcctgcagaa gcagggctcc ggcgatggcg aagcgcgctt catcgaggtc gtgaacactc tcgtgcgctt ccgtgatgag gtgcgcgatg cggcgaaaga ccacaaggtg gtggcgggct tcctgccctt gtgcgacaag gtgcgcgatg agtggcttgt cgacgctggc gtacggctcg aggacaaccc ggcgggcccg acgacgtgga agagcgacga gccggcgctg ctgcacaaag agctggcgga gcgccgcgct cagcaagaag gggatcgcca gaggaggctg gcgaatcagg cggagacgaa gcgcaagctc gtggaaaagt ggcggcagtt cacctacccg ccgtccgagt actttcgcct ccaggacgag caacgggtag agaagaagta cgctgcctac gacgacgtca ccggcctccc aacgatgacg gcggccggcg aggaggtgag cgagaaggag gcaaagaagt tgagcaagga gcaggcgaag tacgccaagt cgtacgacga gtttatcggc aagggtggcg tgtcgtggct tcaagagcag gaggcggagc tggccagcat ggtggccgag ctcaaaggca gcaattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat