LedoB.00063.a

histidyl-tRNA synthetase, putative

CENTER ID: LedoB.00063.a
ORGANISM: Leishmania donovani BPK282A1
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.00063.a.B1.GE42599 full length 1 473
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoB.00063.a.B1.PW38530 full length 1 473

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LdBPK_300650.1
UniProt: E9BLF9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSTASPNAA LLAEINDLKV KLAEKEALLQ PEGVVSKKSK KKSQANMIEK EPVQGCRDFP PEDMRVRRHL FGVFHDTARR FGFEEYDAPV LESEELYIRK AGEEITEQMF NFVTKGGHRV TLRPEMTPSL VRLQLAKGRS LLLPAKWYSI PQCWRYEAIS RGRRREHYQW NMDIVGVKSV AAEVELVCAA CAAMESLGLT AQDVGVKVNS RKLLQCALAG AGVPDSLFAP VCVIVDKMEK LPEEQIRAEL KDLALDGQQA DDIIKTLSLR SVDDFDKDLF DKNGALSELA EFFSQMQMYG YGDWIQFDAS VVRGLAYYTG IVFEAFDRSG EFRAICGGGR YDNLFTLYGS PQPVSCVGFG FGDCVIVELL KKKKLINSLP HKVDDVVIPF NESQRGTALK VLKQLRDKGR SADIIMDSKK VAQTFSYADR IGADRAVLIA PDETEKGMAR VKMLREGQRG EDDRGDPVPF EDL
NT Sequence
atgtcctcga cggcctcgcc caatgccgcg cttctcgcgg aaatcaacga cctgaaggtg aaactcgccg agaaggaggc gctgctgcag ccggaggggg tagtatcgaa gaagtccaag aaaaagagcc aagcgaacat gatcgagaaa gagcccgtgc agggctgccg cgacttccct cccgaggaca tgcgagtgcg caggcacctc ttcggcgtct tccacgatac ggcccgcaga ttcggtttcg aggagtatga cgccccagtt ctagagtcgg aggagctcta catccgcaaa gcgggagagg agattacaga acaaatgttc aactttgtga cgaagggtgg ccaccgtgtc acactgcggc ctgagatgac gccgtcattg gtgcgtttgc agctcgccaa gggccgctct ctgcttctgc ccgccaagtg gtactccatt ccgcagtgct ggcgctacga ggccatctcc cgcggtcgtc gccgtgagca ttaccagtgg aatatggata tcgtcggtgt gaagagcgtg gccgcagagg tggagctcgt gtgcgccgca tgcgcggcga tggagtcgct cggcctcacc gcgcaggacg ttggcgtgaa ggtgaactcg cgcaagttgc tgcagtgtgc actcgccggc gctggcgtgc cggattctct ctttgctcca gtgtgcgtga tcgtcgacaa gatggagaag ctgcctgagg agcaaatccg cgcggagtta aaggacctgg ccctggatgg ccagcaggcg gacgacatca tcaagacact tagtctcagg tctgtcgacg acttcgacaa ggacctcttt gacaagaacg gcgccctctc ggagctcgca gagttcttct cgcagatgca gatgtacggg tacggcgact ggatccagtt cgatgcgagt gtcgtgcgtg gactcgccta ctacaccggc atcgttttcg aagcgtttga ccgcagtggt gagttccgcg cgatctgcgg aggcggccgc tacgacaacc tcttcactct gtacggcagc ccgcagcctg tctcgtgcgt cgggttcggc ttcggcgact gtgtcatcgt tgaactgctg aagaagaaga agctgatcaa ctcgctgccg cataaggtgg atgatgtggt gattccgttc aatgagtctc agcgaggcac cgccctgaag gtattgaagc agctgcgtga caagggccgc agtgccgaca tcattatgga ctccaagaag gttgctcaga ccttcagtta cgcggatcgt atcggcgctg accgtgccgt gctgattgcg ccggacgaaa cggagaaggg catggcgcgc gtgaagatgc tgcgcgaggg gcagaggggt gaggatgacc gcggtgaccc tgtgcccttc gaagacttgt ag
Details for LedoB.00063.a.B1.GE42599
HARVESTED ON: 8/5/2018
SEQUENCED ON: 8/8/2018
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS STASPNAALL AEINDLKVKL AEKEALLQPE GVVSKKSKKK SQANMIEKEP VQGCRDFPPE DMRVRRHLFG VFHDTARRFG FEEYDAPVLE SEELYIRKAG EEITEQMFNF VTKGGHRVTL RPEMTPSLVR LQLAKGRSLL LPAKWYSIPQ CWRYEAISRG RRREHYQWNM DIVGVKSVAA EVELVCAACA AMESLGLTAQ DVGVKVNSRK LLQCALAGAG VPDSLFAPVC VIVDKMEKLP EEQIRAELKD LALDGQQADD IIKTLSLRSV DDFDKDLFDK NGALSELAEF FSQMQMYGYG DWIQFDASVV RGLAYYTGIV FEAFDRSGEF RAICGGGRYD NLFTLYGSPQ PVSCVGFGFG DCVIVELLKK KKLINSLPHK VDDVVIPFNE SQRGTALKVL KQLRDKGRSA DIIMDSKKVA QTFSYADRIG ADRAVLIAPD ETEKGMARVK MLREGQRGED DR
Validated NT Sequence
ccgcggtcat cctcacccct ctgcccctcg cgcagcatct tcacgcgcgc catgcccttc tccgtttcgt ccggcgcaat cagcacggca cggtcagcgc cgatacgatc cgcgtaactg aaggtctgag caaccttctt ggagtccata atgatgtcgg cactgcggcc cttgtcacgc agctgcttca ataccttcag ggcggtgcct cgctgagact cattgaacgg aatcaccaca tcatccacct tatgcggcag cgagttgatc agcttcttct tcttcagcag ttcaacgatg acacagtcgc cgaagccgaa cccgacgcac gagacaggct gcgggctgcc gtacagagtg aagaggttgt cgtagcggcc gcctccgcag atcgcgcgga actcaccact gcggtcaaac gcttcgaaaa cgatgccggt gtagtaggcg agtccacgca cgacactcgc atcgaactgg atccagtcgc cgtacccgta catctgcatc tgcgagaaga actctgcgag ctccgagagg gcgccgttct tgtcaaagag gtccttgtcg aagtcgtcga cagacctgag actaagtgtc ttgatgatgt cgtccgcctg ctggccatcc agggccaggt cctttaactc cgcgcggatt tgctcctcag gcagcttctc catcttgtcg acgatcacgc acactggagc aaagagagaa tccggcacgc cagcgccggc gagtgcacac tgcagcaact tgcgcgagtt caccttcacg ccaacgtcct gcgcggtgag gccgagcgac tccatcgccg cgcatgcggc gcacacgagc tccacctctg cggccacgct cttcacaccg acgatatcca tattccactg gtaatgctca cggcgacgac cgcgggagat ggcctcgtag cgccagcact gcggaatgga gtaccacttg gcgggcagaa gcagagagcg gcccttggcg agctgcaaac gcaccaatga cggcgtcatc tcaggccgca gtgtgacacg gtggccaccc ttcgtcacaa agttgaacat ttgttctgta atctcctctc ccgctttgcg gatgtagagc tcctccgact ctagaactgg ggcgtcatac tcctcgaaac cgaatctgcg ggccgtatcg tggaagacgc cgaagaggtg cctgcgcact cgcatgtcct cgggagggaa gtcgcggcag ccctgcacgg gctctttctc gatcatgttc gcttggctct ttttcttgga cttcttcgat actaccccct ccggctgcag cagcgcctcc ttctcggcga gtttcacctt caggtcgttg atttccgcga gaagcgcggc attgggcgag gccgtcgagg acatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMS STASPNAALL AEINDLKVKL AEKEALLQPE GVVSKKSKKK SQANMIEKEP VQGCRDFPPE DMRVRRHLFG VFHDTARRFG FEEYDAPVLE SEELYIRKAG EEITEQMFNF VTKGGHRVTL RPEMTPSLVR LQLAKGRSLL LPAKWYSIPQ CWRYEAISRG RRREHYQWNM DIVGVKSVAA EVELVCAACA AMESLGLTAQ DVGVKVNSRK LLQCALAGAG VPDSLFAPVC VIVDKMEKLP EEQIRAELKD LALDGQQADD IIKTLSLRSV DDFDKDLFDK NGALSELAEF FSQMQMYGYG DWIQFDASVV RGLAYYTGIV FEAFDRSGEF RAICGGGRYD NLFTLYGSPQ PVSCVGFGFG DCVIVELLKK KKLINSLPHK VDDVVIPFNE SQRGTALKVL KQLRDKGRSA DIIMDSKKVA QTFSYADRIG ADRAVLIAPD ETEKGMARVK MLREGQRGED DRGDPVPFED L
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcct cgacggcctc gcccaatgcc gcgcttctcg cggaaatcaa cgacctgaag gtgaaactcg ccgagaagga ggcgctgctg cagccggagg gggtagtatc gaagaagtcc aagaaaaaga gccaagcgaa catgatcgag aaagagcccg tgcagggctg ccgcgacttc cctcccgagg acatgcgagt gcgcaggcac ctcttcggcg tcttccacga tacggcccgc agattcggtt tcgaggagta tgacgcccca gttctagagt cggaggagct ctacatccgc aaagcgggag aggagattac agaacaaatg ttcaactttg tgacgaaggg tggccaccgt gtcacactgc ggcctgagat gacgccgtca ttggtgcgtt tgcagctcgc caagggccgc tctctgcttc tgcccgccaa gtggtactcc attccgcagt gctggcgcta cgaggccatc tcccgcggtc gtcgccgtga gcattaccag tggaatatgg atatcgtcgg tgtgaagagc gtggccgcag aggtggagct cgtgtgcgcc gcatgcgcgg cgatggagtc gctcggcctc accgcgcagg acgttggcgt gaaggtgaac tcgcgcaagt tgctgcagtg tgcactcgcc ggcgctggcg tgccggattc tctctttgct ccagtgtgcg tgatcgtcga caagatggag aagctgcctg aggagcaaat ccgcgcggag ttaaaggacc tggccctgga tggccagcag gcggacgaca tcatcaagac acttagtctc aggtctgtcg acgacttcga caaggacctc tttgacaaga acggcgccct ctcggagctc gcagagttct tctcgcagat gcagatgtac gggtacggcg actggatcca gttcgatgcg agtgtcgtgc gtggactcgc ctactacacc ggcatcgttt tcgaagcgtt tgaccgcagt ggtgagttcc gcgcgatctg cggaggcggc cgctacgaca acctcttcac tctgtacggc agcccgcagc ctgtctcgtg cgtcgggttc ggcttcggcg actgtgtcat cgttgaactg ctgaagaaga agaagctgat caactcgctg ccgcataagg tggatgatgt ggtgattccg ttcaatgagt ctcagcgagg caccgccctg aaggtattga agcagctgcg tgacaagggc cgcagtgccg acatcattat ggactccaag aaggttgctc agaccttcag ttacgcggat cgtatcggcg ctgaccgtgc cgtgctgatt gcgccggacg aaacggagaa gggcatggcg cgcgtgaaga tgctgcgcga ggggcagagg ggtgaggatg accgcggtga ccctgtgccc ttcgaagact tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for LedoB.00063.a.B1.PW38530
PURIFICATION DATe: 9/25/2018
CONCENTRATION: 10.55mg/ml
OBSERVED MW: 54kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS STASPNAALL AEINDLKVKL AEKEALLQPE GVVSKKSKKK SQANMIEKEP VQGCRDFPPE DMRVRRHLFG VFHDTARRFG FEEYDAPVLE SEELYIRKAG EEITEQMFNF VTKGGHRVTL RPEMTPSLVR LQLAKGRSLL LPAKWYSIPQ CWRYEAISRG RRREHYQWNM DIVGVKSVAA EVELVCAACA AMESLGLTAQ DVGVKVNSRK LLQCALAGAG VPDSLFAPVC VIVDKMEKLP EEQIRAELKD LALDGQQADD IIKTLSLRSV DDFDKDLFDK NGALSELAEF FSQMQMYGYG DWIQFDASVV RGLAYYTGIV FEAFDRSGEF RAICGGGRYD NLFTLYGSPQ PVSCVGFGFG DCVIVELLKK KKLINSLPHK VDDVVIPFNE SQRGTALKVL KQLRDKGRSA DIIMDSKKVA QTFSYADRIG ADRAVLIAPD ETEKGMARVK MLREGQRGED DR
Validated NT Sequence
ccgcggtcat cctcacccct ctgcccctcg cgcagcatct tcacgcgcgc catgcccttc tccgtttcgt ccggcgcaat cagcacggca cggtcagcgc cgatacgatc cgcgtaactg aaggtctgag caaccttctt ggagtccata atgatgtcgg cactgcggcc cttgtcacgc agctgcttca ataccttcag ggcggtgcct cgctgagact cattgaacgg aatcaccaca tcatccacct tatgcggcag cgagttgatc agcttcttct tcttcagcag ttcaacgatg acacagtcgc cgaagccgaa cccgacgcac gagacaggct gcgggctgcc gtacagagtg aagaggttgt cgtagcggcc gcctccgcag atcgcgcgga actcaccact gcggtcaaac gcttcgaaaa cgatgccggt gtagtaggcg agtccacgca cgacactcgc atcgaactgg atccagtcgc cgtacccgta catctgcatc tgcgagaaga actctgcgag ctccgagagg gcgccgttct tgtcaaagag gtccttgtcg aagtcgtcga cagacctgag actaagtgtc ttgatgatgt cgtccgcctg ctggccatcc agggccaggt cctttaactc cgcgcggatt tgctcctcag gcagcttctc catcttgtcg acgatcacgc acactggagc aaagagagaa tccggcacgc cagcgccggc gagtgcacac tgcagcaact tgcgcgagtt caccttcacg ccaacgtcct gcgcggtgag gccgagcgac tccatcgccg cgcatgcggc gcacacgagc tccacctctg cggccacgct cttcacaccg acgatatcca tattccactg gtaatgctca cggcgacgac cgcgggagat ggcctcgtag cgccagcact gcggaatgga gtaccacttg gcgggcagaa gcagagagcg gcccttggcg agctgcaaac gcaccaatga cggcgtcatc tcaggccgca gtgtgacacg gtggccaccc ttcgtcacaa agttgaacat ttgttctgta atctcctctc ccgctttgcg gatgtagagc tcctccgact ctagaactgg ggcgtcatac tcctcgaaac cgaatctgcg ggccgtatcg tggaagacgc cgaagaggtg cctgcgcact cgcatgtcct cgggagggaa gtcgcggcag ccctgcacgg gctctttctc gatcatgttc gcttggctct ttttcttgga cttcttcgat actaccccct ccggctgcag cagcgcctcc ttctcggcga gtttcacctt caggtcgttg atttccgcga gaagcgcggc attgggcgag gccgtcgagg acatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMS STASPNAALL AEINDLKVKL AEKEALLQPE GVVSKKSKKK SQANMIEKEP VQGCRDFPPE DMRVRRHLFG VFHDTARRFG FEEYDAPVLE SEELYIRKAG EEITEQMFNF VTKGGHRVTL RPEMTPSLVR LQLAKGRSLL LPAKWYSIPQ CWRYEAISRG RRREHYQWNM DIVGVKSVAA EVELVCAACA AMESLGLTAQ DVGVKVNSRK LLQCALAGAG VPDSLFAPVC VIVDKMEKLP EEQIRAELKD LALDGQQADD IIKTLSLRSV DDFDKDLFDK NGALSELAEF FSQMQMYGYG DWIQFDASVV RGLAYYTGIV FEAFDRSGEF RAICGGGRYD NLFTLYGSPQ PVSCVGFGFG DCVIVELLKK KKLINSLPHK VDDVVIPFNE SQRGTALKVL KQLRDKGRSA DIIMDSKKVA QTFSYADRIG ADRAVLIAPD ETEKGMARVK MLREGQRGED DRGDPVPFED L
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCT CGACGGCCTC GCCCAATGCC GCGCTTCTCG CGGAAATCAA CGACCTGAAG GTGAAACTCG CCGAGAAGGA GGCGCTGCTG CAGCCGGAGG GGGTAGTATC GAAGAAGTCC AAGAAAAAGA GCCAAGCGAA CATGATCGAG AAAGAGCCCG TGCAGGGCTG CCGCGACTTC CCTCCCGAGG ACATGCGAGT GCGCAGGCAC CTCTTCGGCG TCTTCCACGA TACGGCCCGC AGATTCGGTT TCGAGGAGTA TGACGCCCCA GTTCTAGAGT CGGAGGAGCT CTACATCCGC AAAGCGGGAG AGGAGATTAC AGAACAAATG TTCAACTTTG TGACGAAGGG TGGCCACCGT GTCACACTGC GGCCTGAGAT GACGCCGTCA TTGGTGCGTT TGCAGCTCGC CAAGGGCCGC TCTCTGCTTC TGCCCGCCAA GTGGTACTCC ATTCCGCAGT GCTGGCGCTA CGAGGCCATC TCCCGCGGTC GTCGCCGTGA GCATTACCAG TGGAATATGG ATATCGTCGG TGTGAAGAGC GTGGCCGCAG AGGTGGAGCT CGTGTGCGCC GCATGCGCGG CGATGGAGTC GCTCGGCCTC ACCGCGCAGG ACGTTGGCGT GAAGGTGAAC TCGCGCAAGT TGCTGCAGTG TGCACTCGCC GGCGCTGGCG TGCCGGATTC TCTCTTTGCT CCAGTGTGCG TGATCGTCGA CAAGATGGAG AAGCTGCCTG AGGAGCAAAT CCGCGCGGAG TTAAAGGACC TGGCCCTGGA TGGCCAGCAG GCGGACGACA TCATCAAGAC ACTTAGTCTC AGGTCTGTCG ACGACTTCGA CAAGGACCTC TTTGACAAGA ACGGCGCCCT CTCGGAGCTC GCAGAGTTCT TCTCGCAGAT GCAGATGTAC GGGTACGGCG ACTGGATCCA GTTCGATGCG AGTGTCGTGC GTGGACTCGC CTACTACACC GGCATCGTTT TCGAAGCGTT TGACCGCAGT GGTGAGTTCC GCGCGATCTG CGGAGGCGGC CGCTACGACA ACCTCTTCAC TCTGTACGGC AGCCCGCAGC CTGTCTCGTG CGTCGGGTTC GGCTTCGGCG ACTGTGTCAT CGTTGAACTG CTGAAGAAGA AGAAGCTGAT CAACTCGCTG CCGCATAAGG TGGATGATGT GGTGATTCCG TTCAATGAGT CTCAGCGAGG CACCGCCCTG AAGGTATTGA AGCAGCTGCG TGACAAGGGC CGCAGTGCCG ACATCATTAT GGACTCCAAG AAGGTTGCTC AGACCTTCAG TTACGCGGAT CGTATCGGCG CTGACCGTGC CGTGCTGATT GCGCCGGACG AAACGGAGAA GGGCATGGCG CGCGTGAAGA TGCTGCGCGA GGGGCAGAGG GGTGAGGATG ACCGCGGTGA CCCTGTGCCC TTCGAAGACT TGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT