SSGCID: Target Page

LedoA.19310.a

Uncharacterized protein (Pi4Ka)

CENTER ID:	LedoA.19310.a
ORGANISM:	Leishmania donovani
ASSOCIATED DISEASE:	Leishmaniasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LedoA.19310.a.B1.GE42074	full length		1	546

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LDBPK_044320
UniProt:	E9BRN1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPLNRELLAS VKLEPMSDSD WVEEGHDKTN DGRSAGADGS VHPASDADES EDQNAATKNV IHCLKIALER GITSISLPEK QRPLNPQKDF SIESTMHFSA SRPAKGITCC GAQATGVKKA RNPQMRDARS SIIGGVYRQA NRPRNDMSQI LSTDNAILNS DGDMDRDYGG LATSGANFDT SVARARRAHR SLTRTQTLAD FSSDDEDDED EENMPPVSFT FTDFSPMCYR HIREFFNVDP KAYCDVLRNS RWHSIPTPGK SAAQLFFCGR DWVIKTMTEQ ESDFLRKILH RYYYHVRDNP FTLLPHFVGH HRLRIGTKTQ NFIIMQNVFA TTNTIHEKFD LKGSTIGRFA SDAEKRRTTF TQKDLDINSP MHIGPERRNL LIEQIKKDCE FLKRSMIMDY SFLVGIHVLP SVGDTLSSAT SGGSLGMLTR SVTDGTLRTN PGLGYTMGGE RPDNSGADGS EFSRNGSRLD GRCFTADQGG MMSNKVPGLR QEIYYIGIID ILQEYNARKA LENVVWGSLY DRKRISCVHP NDYAGRFIAF MSSIIV

NT Sequence

atgcccctta atcgagagct gttggcgtcc gtgaagctgg agcccatgag tgactcggac tgggtggagg agggacatga taaaacgaat gacggtcgct cagccggcgc ggacggctcc gtgcaccccg cctctgacgc cgacgagagt gaggaccaaa acgcagccac caagaatgtg attcactgtc tgaaaatagc gctagaacgt ggcatcacga gtatctcgct gcccgagaag cagcggccgt taaacccgca gaaggacttc tcgatcgagt ccaccatgca cttcagcgcg tctcggcccg ccaaggggat tacctgctgc ggcgcccaag ccacaggtgt gaagaaggcg cgcaacccgc agatgagaga tgcccgctcg tccataatcg gtggtgtcta tcggcaagca aaccgtccac ggaatgatat gtcgcagatt ctctccacag acaatgccat tttgaactcc gacggcgaca tggaccggga ctacggcggt ttggcgactt ctggtgcaaa cttcgataca tctgtcgcac gcgcgcgccg tgcccaccgc tcactgacaa ggacgcagac gctggcggac ttctcgtcgg atgatgagga cgacgaagac gaggagaaca tgccaccggt gagctttacc tttacagatt tctcgcccat gtgctaccgc cacatccgcg agttcttcaa cgtagatcca aaggcgtact gcgatgtgct gcgcaacagc cgctggcaca gcatcccaac gccaggcaag tcggctgcgc aacttttttt ctgcggtcgc gattgggtga tcaagacaat gacggagcag gagagcgact ttctgcgcaa gatcctgcac cggtattatt atcacgtgcg cgacaaccct ttcaccttgc tcccacactt cgtgggccac catcgccttc gcatcggcac gaaaacgcaa aacttcatca ttatgcaaaa cgtctttgcc accaccaaca cgatccatga gaagttcgac ctgaagggca gcaccatcgg ccgctttgcc tcggacgctg agaagcgccg gacaacgttt acgcagaagg acctcgatat aaacagcccg atgcatattg gcccggagcg gcggaatctg ttgatcgagc aaatcaagaa ggattgcgag tttctgaagc gatccatgat catggactac tcattcctgg ttggcataca cgtgttgccg tcggtggggg acaccttgtc ctccgcgacc tctggagggt cgctagggat gctgacacgc agtgtaacgg acggcacact gcgcacaaac ccgggtctgg ggtacaccat gggaggggag cggccagata attccggggc tgatggctct gaattctcgc gcaacgggtc caggctggac ggccgctgct tcacggctga tcaggggggc atgatgagca acaaggtgcc tgggctgcga caggagatat actacatcgg catcattgac attttgcagg agtacaacgc gcgtaaagcg ctcgagaatg ttgtgtgggg gtccttgtac gatcgaaagc gcatctcgtg cgtccacccg aacgactacg caggacgatt catcgcgttc atgtcatcca tcattgtcta g

Details for LedoA.19310.a.B1.GE42074

HARVESTED ON:	12/5/2017
SEQUENCED ON:	12/7/2017
EXPECTED MW:	62kDa
OBSERVED MW:	62kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMP LNRELLASVK LEPMSDSDWV EEGHDKTNDG RSAGADGSVH PASDADESED QNAATKNVIH CLKIALERGI TSISLPEKQR PLNPQKDFSI ESTMHFSASR PAKGITCCGA QATGVKKARN PQMRDARSSI IGGVYRQANR PRNDMSQILS TDNAILNSDG DMDRDYGGLA TSGANFDTSV ARARRAHRSL TRTQTLADFS SDDEDDEDEE NMPPVSFTFT DFSPMCYRHI REFFNVDPKA YCDVLRNSRW HSIPTPGKSA AQLFFCGRDW VIKTMTEQES DFLRKILHRY YYHVRDNPFT LLPHFVGHHR LRIGTKTQNF IIMQNVFATT NTIHEKFDLK GSTIGRFASD AEKRRTTFTQ KDLDINSPMH IGPERRNLLI EQIKKDCEFL KRSMIMDYSF LVGIHVLPSV GDTLSSATSG GSLGMLTRSV TDGTLRTNPG LGYTMGGERP DNSGADGSEF SRNGSRLDGR CFTADQGGMM SNKVPGLRQE IYYIGIIDIL QEYNARKALE NVVWGSLYDR KRISCVHPND YAGRFI

Validated NT Sequence

cgcgatgaat cgtcctgcgt agtcgttcgg gtggacgcac gagatgcgct ttcgatcgta caaggacccc cacacaacat tctcgagcgc tttacgcgcg ttgtactcct gcaaaatgtc aatgatgccg atgtagtata tctcctgtcg cagcccaggc accttgttgc tcatcatgcc cccctgatca gccgtgaagc agcggccgtc cagcctggac ccgttgcgcg agaattcaga gccatcagcc ccggaattat ctggccgctc ccctcccatg gtgtacccca gacccgggtt tgtgcgcagt gtgccgtccg ttacactgcg tgtcagcatc cctagcgacc ctccagaggt cgcggaggac aaggtgtccc ccaccgacgg caacacgtgt atgccaacca ggaatgagta gtccatgatc atggatcgct tcagaaactc gcaatccttc ttgatttgct cgatcaacag attccgccgc tccgggccaa tatgcatcgg gctgtttata tcgaggtcct tctgcgtaaa cgttgtccgg cgcttctcag cgtccgaggc aaagcggccg atggtgctgc ccttcaggtc gaacttctca tggatcgtgt tggtggtggc aaagacgttt tgcataatga tgaagttttg cgttttcgtg ccgatgcgaa ggcgatggtg gcccacgaag tgtgggagca aggtgaaagg gttgtcgcgc acgtgataat aataccggtg caggatcttg cgcagaaagt cgctctcctg ctccgtcatt gtcttgatca cccaatcgcg accgcagaaa aaaagttgcg cagccgactt gcctggcgtt gggatgctgt gccagcggct gttgcgcagc acatcgcagt acgcctttgg atctacgttg aagaactcgc ggatgtggcg gtagcacatg ggcgagaaat ctgtaaaggt aaagctcacc ggtggcatgt tctcctcgtc ttcgtcgtcc tcatcatccg acgagaagtc cgccagcgtc tgcgtccttg tcagtgagcg gtgggcacgg cgcgcgcgtg cgacagatgt atcgaagttt gcaccagaag tcgccaaacc gccgtagtcc cggtccatgt cgccgtcgga gttcaaaatg gcattgtctg tggagagaat ctgcgacata tcattccgtg gacggtttgc ttgccgatag acaccaccga ttatggacga gcgggcatct ctcatctgcg ggttgcgcgc cttcttcaca cctgtggctt gggcgccgca gcaggtaatc cccttggcgg gccgagacgc gctgaagtgc atggtggact cgatcgagaa gtccttctgc gggtttaacg gccgctgctt ctcgggcagc gagatactcg tgatgccacg ttctagcgct attttcagac agtgaatcac attcttggtg gctgcgtttt ggtcctcact ctcgtcggcg tcggaggcgg ggtgcacgga gccgtccgcg ccggctgagc gaccgtcatt cgttttatca tgtccctcct ccacccagtc cgagtcactc atgggctcca gcttcacgga cgccaacagc tctcgattaa ggggcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccc ttaatcgaga gctgttggcg tccgtgaagc tggagcccat gagtgactcg gactgggtgg aggagggaca tgataaaacg aatgacggtc gctcagccgg cgcggacggc tccgtgcacc ccgcctctga cgccgacgag agtgaggacc aaaacgcagc caccaagaat gtgattcact gtctgaaaat agcgctagaa cgtggcatca cgagtatctc gctgcccgag aagcagcggc cgttaaaccc gcagaaggac ttctcgatcg agtccaccat gcacttcagc gcgtctcggc ccgccaaggg gattacctgc tgcggcgccc aagccacagg tgtgaagaag gcgcgcaacc cgcagatgag agatgcccgc tcgtccataa tcggtggtgt ctatcggcaa gcaaaccgtc cacggaatga tatgtcgcag attctctcca cagacaatgc cattttgaac tccgacggcg acatggaccg ggactacggc ggtttggcga cttctggtgc aaacttcgat acatctgtcg cacgcgcgcg ccgtgcccac cgctcactga caaggacgca gacgctggcg gacttctcgt cggatgatga ggacgacgaa gacgaggaga acatgccacc ggtgagcttt acctttacag atttctcgcc catgtgctac cgccacatcc gcgagttctt caacgtagat ccaaaggcgt actgcgatgt gctgcgcaac agccgctggc acagcatccc aacgccaggc aagtcggctg cgcaactttt tttctgcggt cgcgattggg tgatcaagac aatgacggag caggagagcg actttctgcg caagatcctg caccggtatt attatcacgt gcgcgacaac cctttcacct tgctcccaca cttcgtgggc caccatcgcc ttcgcatcgg cacgaaaacg caaaacttca tcattatgca aaacgtcttt gccaccacca acacgatcca tgagaagttc gacctgaagg gcagcaccat cggccgcttt gcctcggacg ctgagaagcg ccggacaacg tttacgcaga aggacctcga tataaacagc ccgatgcata ttggcccgga gcggcggaat ctgttgatcg agcaaatcaa gaaggattgc gagtttctga agcgatccat gatcatggac tactcattcc tggttggcat acacgtgttg ccgtcggtgg gggacacctt gtcctccgcg acctctggag ggtcgctagg gatgctgaca cgcagtgtaa cggacggcac actgcgcaca aacccgggtc tggggtacac catgggaggg gagcggccag ataattccgg ggctgatggc tctgaattct cgcgcaacgg gtccaggctg gacggccgct gcttcacggc tgatcagggg ggcatgatga gcaacaaggt gcctgggctg cgacaggaga tatactacat cggcatcatt gacattttgc aggagtacaa cgcgcgtaaa gcgctcgaga atgttgtgtg ggggtccttg tacgatcgaa agcgcatctc gtgcgtccac ccgaacgact acgcaggacg attcatcgcg ttcatgtcat ccatcattgt ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat