SSGCID: Target Page

LedoA.18219.a

Glycylpeptide N-tetradecanoyltransferase (EC 2.3.1.97) (N-myristoyl transferase) (NMT)

CENTER ID:	LedoA.18219.a
ORGANISM:	Leishmania donovani
ASSOCIATED DISEASE:	Leishmaniasis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LedoA.18219.a.B1.GE42071	full length		1	421

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LDBPK_320080
UniProt:	E9BNB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSRNPSNSDA AHAFWSTQPV PQTEDETEKI VFAGPMDEPK TVADIPEEPY PIASTFEWWT PNMEAADDIH AIYELLRDNY VEDDDSMFRF NYSEEFLQWA LCPPSYIPDW HVAVRRKADK KLLAFIAGVP VTLRMGTPKY MKVKAQEKGQ EEEAAKYDAP RHICEINFLC VHKQLREKRL APILIKEVTR RVNRTNVWQA VYTAGVLLPT PYASGQYFHR SLNPEKLVEI RFSGIPAQYQ KFQNPMAMLK RNYQLPNAPK NSGLREMKPS DVPQVRRILM NYLDNFDVGP VFSDAEISHY LLPRDGVVFT YVVENDKKVT DFFSFYRIPS TVIGNSNYNI LNAAYVHYYA ATSMPLHQLI LDLLIVAHSR GFDVCNMVEI LDNRSFVEQL KFGAGDGHLR YYFYNWGYPK IKPSRVALVM L

NT Sequence

atgtctcgca atccatcgaa ctctgacgct gcgcatgcat tctggagcac acagcccgta ccgcagacgg aagatgagac ggagaaaatc gtgttcgccg gtccgatgga cgagccaaaa acggtagccg atattcctga ggaaccatac ccgatcgcca gcacatttga gtggtggacg ccaaacatgg aggcggccga tgacattcac gcaatttacg agcttctccg cgataactac gtcgaggacg acgacagcat gtttcgtttc aactactccg aggagtttct tcagtgggca ctatgcccac cgagctacat cccggactgg cacgttgccg ttcgccggaa ggcggataag aagctgctgg ccttcattgc cggcgttccc gtgacgttgc gcatgggcac ccccaagtac atgaaagtga aagcacagga aaagggccaa gaggaggagg cggccaagta tgatgcaccc cgtcacatct gcgaaatcaa ctttctctgt gtccacaagc aactgcggga gaagcggctt gcccctattt tgatcaaaga ggtgacgcgc cgcgtgaacc gcaccaacgt gtggcaggcg gtgtacacag ctggtgtgct gctacccact ccgtatgcat cagggcagta cttccaccgc agcctgaacc ccgagaagct tgtggagatc cgcttcagcg gcattcctgc acagtaccag aagtttcaga acccaatggc gatgctgaag cgcaactacc agctgccgaa cgcgccaaag aactctggtc ttcgtgaaat gaagccatct gacgttccgc aggtacggcg aattctcatg aactacctgg acaacttcga tgtaggtccc gtctttagcg atgccgagat cagccactac ctccttccgc gcgacggtgt ggtcttcacc tacgtggtgg aaaacgataa gaaggtgacg gacttctttt ccttctatcg aattccatcg accgtgattg ggaacagcaa ttataacatt ctgaacgcag cttacgttca ctactatgcg gcaacgagta tgcctttgca tcaactcatt ctcgaccttt tgatcgtggc gcactcacgc ggcttcgacg tgtgcaatat ggtagagatc ctcgacaacc ggtcgttcgt tgagcagctc aagtttggcg ccggcgacgg tcatcttcgc tattacttct acaactgggg gtatccaaag atcaagcctt ctcgggttgc cttggtgatg ttgtag

Details for LedoA.18219.a.B1.GE42071

HARVESTED ON:	data unavailable
SEQUENCED ON:	12/7/2017
EXPECTED MW:	50kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS RNPSNSDAAH AFWSTQPVPQ TEDETEKIVF AGPMDEPKTV ADIPEEPYPI ASTFEWWTPN MEAADDIHAI YELLRDNYVE DDDSMFRFNY SEEFLQWALC PPSYIPDWHV AVRRKADKKL LAFIAGVPVT LRMGTPKYMK VKAQEKGQEE EAAKYDAPRH ICEINFLCVH KQLREKRLAP ILIKEVTRRV NRTNVWQAVY TAGVLLPTPY ASGQYFHRSL NPEKLVEIRF SGIPAQYQKF QNPMAMLKRN YQLPNAPKNS GLREMKPSDV PQVRRILMNY LDNFDVGPVF SDAEISHYLL PRDGVVFTYV VENDKKVTDF FSFYRIPSTV IGNSNYNILN AAYVHYYAAT SMPLHQLILD LLIVAHSRGF DVCNMVEILD NRSFVEQLKF GAGDGHLRYY FYNWAYPKIK PSRVALVML

Validated NT Sequence

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctc gcaatccatc gaactctgac gctgcgcatg cattctggag cacacagccc gtaccgcaga cggaagatga gacggagaaa atcgtgttcg ccggtccgat ggacgagcca aaaacggtag ccgatattcc tgaggaacca tacccgatcg ccagcacatt tgagtggtgg acgccaaaca tggaggcggc cgatgacatt cacgcaattt acgagcttct ccgcgataac tacgtcgagg acgacgacag catgtttcgt ttcaactact ccgaggagtt tcttcagtgg gcactatgcc caccgagcta catcccggac tggcacgttg ccgttcgccg gaaggcggat aagaagctgc tggccttcat tgccggcgtt cccgtgacgt tgcgcatggg cacccccaag tacatgaaag tgaaagcaca ggaaaagggc caagaggagg aggcggccaa gtatgatgca ccccgtcaca tctgcgaaat caactttctc tgtgtccaca agcaactgcg ggagaagcgg cttgccccta ttttgatcaa agaggtgacg cgccgcgtga accgcaccaa cgtgtggcag gcggtgtaca cagctggtgt gctgctaccc actccgtatg catcagggca gtacttccac cgcagcctga accccgagaa gcttgtggag atccgcttca gcggcattcc tgcacagtac cagaagtttc agaacccaat ggcgatgctg aagcgcaact accagctgcc gaacgcgcca aagaactctg gtcttcgtga aatgaagcca tctgacgttc cgcaggtacg gcgaattctc atgaactacc tggacaactt cgatgtaggt cccgtcttta gcgatgccga gatcagccac tacctccttc cgcgcgacgg tgtggtcttc acctacgtgg tggaaaacga taagaaggtg acggacttct tttccttcta tcgaattcca tcgaccgtga ttgggaacag caattataac attctgaacg cagcttacgt tcactactat gcggcaacga gtatgccttt gcatcaactc attctcgacc ttttgatcgt ggcgcactca cgcggcttcg acgtgtgcaa tatggtagag atcctcgaca accggtcgtt cgttgagcag ctcaagtttg gcgccggcga cggtcatctt cgctattact tctacaactg ggcgtatcca aagatcaagc cttctcgggt tgccttggtg atgttgtaag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctc gcaatccatc gaactctgac gctgcgcatg cattctggag cacacagccc gtaccgcaga cggaagatga gacggagaaa atcgtgttcg ccggtccgat ggacgagcca aaaacggtag ccgatattcc tgaggaacca tacccgatcg ccagcacatt tgagtggtgg acgccaaaca tggaggcggc cgatgacatt cacgcaattt acgagcttct ccgcgataac tacgtcgagg acgacgacag catgtttcgt ttcaactact ccgaggagtt tcttcagtgg gcactatgcc caccgagcta catcccggac tggcacgttg ccgttcgccg gaaggcggat aagaagctgc tggccttcat tgccggcgtt cccgtgacgt tgcgcatggg cacccccaag tacatgaaag tgaaagcaca ggaaaagggc caagaggagg aggcggccaa gtatgatgca ccccgtcaca tctgcgaaat caactttctc tgtgtccaca agcaactgcg ggagaagcgg cttgccccta ttttgatcaa agaggtgacg cgccgcgtga accgcaccaa cgtgtggcag gcggtgtaca cagctggtgt gctgctaccc actccgtatg catcagggca gtacttccac cgcagcctga accccgagaa gcttgtggag atccgcttca gcggcattcc tgcacagtac cagaagtttc agaacccaat ggcgatgctg aagcgcaact accagctgcc gaacgcgcca aagaactctg gtcttcgtga aatgaagcca tctgacgttc cgcaggtacg gcgaattctc atgaactacc tggacaactt cgatgtaggt cccgtcttta gcgatgccga gatcagccac tacctccttc cgcgcgacgg tgtggtcttc acctacgtgg tggaaaacga taagaaggtg acggacttct tttccttcta tcgaattcca tcgaccgtga ttgggaacag caattataac attctgaacg cagcttacgt tcactactat gcggcaacga gtatgccttt gcatcaactc attctcgacc ttttgatcgt ggcgcactca cgcggcttcg acgtgtgcaa tatggtagag atcctcgaca accggtcgtt cgttgagcag ctcaagtttg gcgccggcga cggtcatctt cgctattact tctacaactg ggggtatcca aagatcaagc cttctcgggt tgccttggtg atgttgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t