LedoA.01010.a

Protein kinase, putative (CRK12)

CENTER ID: LedoA.01010.a
ORGANISM: Leishmania donovani
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoA.01010.a.B1.GE42070 full length 1 478
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LDBPK_090270
UniProt: E9B9R3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGTSGFSRLR SLTPTPYPGT QGSPYRRHRF VGVNLPGAPP PSIDFSSIQR TVKEVYEVHE KLSEGTYGEV FKGVDKRTGA AVALKRIKML STHQGFPQTS LREVIALRHI QNQRERLEER LRNDAHHRGA VAITDPLAEV SQLCDVLLYD RQQRDIVLVF AYATASLAGL CRRQFAFTPS EMALLMKKLL IAVRKLHEMR IIHRDIKSDN VLVTSEGEVQ LTDFGLCSIA APGSSRCGTH VWRTPSVITL AYRPPEMLLG STAYDEKVDV WSLGCLLAQM FLLEPPFYRH RAQAQQQQQR APERSAATEL EQLSRITEIL GPLPPVSVYH PDSCQHMRVL EQLEVQGRLA EAGRAAQPAN WGRLQTIFEP SFLYQQFHGF RGWFEAELGR SRHQPHRRPT QACMDVLCAA LQLDPQQRPT AAELLRMPYF TTLDDAPLLG SYQRVLPVTP EREAEVRRGF MTKVQRCGDS HTQRRPHQ
NT Sequence
atggggacaa gcggcttctc acgcctacgc tcgcttacac ccacgccgta tcccggcacg caagggtctc cataccgtcg tcaccgcttt gtgggggtga acctccccgg tgctccgcca ccgtccattg acttcagctc cattcagcgc accgtgaagg aagtctatga agtgcacgag aagctcagcg agggcacgta cggcgaggtg ttcaagggcg tcgacaagcg aaccggcgct gcggtggcgc tgaagcgcat caagatgctg agcacccatc aaggatttcc ccagacctcg ctgcgggagg tgattgccct tcggcacatc cagaaccagc gcgagcggct cgaagaacga ctgcgcaacg acgcgcatca ccgtggcgcg gtggccatca cggatcctct cgctgaggtc tcgcaactgt gtgatgttct cctctacgat cggcagcagc gcgacattgt gttggtgttc gcgtacgcca cagcgagtct ggctggcctg tgtcgccgcc agttcgcctt cacaccatcg gagatggccc ttctcatgaa gaagcttctc atcgcggtgc gcaagctgca cgagatgcgc atcattcacc gggacatcaa gtctgacaac gtactcgtaa cgagtgaggg cgaggtgcag ctgaccgact ttgggctgtg ctccattgca gcgcctgggt cgtcgcgatg tggcacgcat gtgtggcgga cgccgagcgt gattactctg gcctaccgtc cgccagagat gctgttgggc agtaccgcgt atgatgagaa ggtcgatgtg tggtcgcttg ggtgcctgct cgctcagatg ttcctcctcg agcccccctt ctacaggcac cgcgcgcagg cgcagcagca gcagcaacgt gctccagagc gatcggcggc cacggagctc gagcagctct cccgcattac agagattctg gggccactgc cgccggtgag cgtttatcac ccggactcgt gtcagcacat gcgggttcta gagcagctgg aagtgcaggg gcgcctggcg gaggctgggc gagcagcaca accggcaaac tggggcaggc tgcagaccat cttcgagccc agctttctgt atcagcagtt ccacggcttt cggggctggt ttgaggcgga gttggggcgc tcgcggcacc agccacatcg tcggcccacg caggcttgca tggatgtgct ctgcgcggcc ctgcaactcg acccgcagca gcgtcccacc gcggcagagc tgctgcgcat gccgtacttt accaccttgg acgacgcccc gttgctgggc agctatcaac gggtcctccc cgtcaccccg gagcgggaag ctgaggtacg ccgcggtttc atgacaaagg tgcaacgatg cggggacagt cacacgcagc gccgcccgca tcagtga
Details for LedoA.01010.a.B1.GE42070
HARVESTED ON: data unavailable
SEQUENCED ON: 12/7/2017
EXPECTED MW: 55kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TSGFSRLRSL TPTPYPGTQG SPYRRHRFVG VNLPGAPPPS IDFSSIQRTV KEVYEVHEKL SEGTYGEVFK GVDKRTGAAV ALKRIKMLST HQGFPQTSLR EVIALRHIQN QRERLEERLR NDAHHRGAVA ITDPLAEVSQ LCDVLLYDRQ QRDIVLVFAY ATASLAGLCR RQFAFTPSEM ALLMKKLLIA VRKLHEMRII HRDIKSDNVL VTSEGEVQLT DFGLCSIAAP GSSRCGTHVW RTPSVITLAY RPPEMLLGST AYDEKVDVWS LGCLLAQMFL LEPPFYRHRA QAQQQQQRAP ERSAATELEQ LSRITEILGP LPPVSVYHPD SCQHMRVLEQ LEVQGRLAEA GRAAQPANWG RLQTIFEPSF LYQQFHGFRG WFEAELGRSR HQPHRRPTQA CMDVLCAALQ LDPQQRPTAA ELLRMPYFTT LDDAPLLGSY QRVLPVTPER EAEVRRGFMT KVQRCGDSHT QRRPHQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggga caagcggctt ctcacgccta cgctcgctta cacccacgcc gtatcccggc acgcaagggt ctccataccg tcgtcaccgc tttgtggggg tgaacctccc cggtgctccg ccaccgtcca ttgacttcag ctccattcag cgcaccgtga aggaagtcta tgaagtgcac gagaagctca gcgagggcac gtacggcgag gtgttcaagg gcgtcgacaa gcgaaccggc gctgcggtgg cgctgaagcg catcaagatg ctgagcaccc atcaaggatt tccccagacc tcgctgcggg aggtgattgc ccttcggcac atccagaacc agcgcgagcg gctcgaagaa cgactgcgca acgacgcgca tcaccgtggc gcggtggcca tcacggatcc tctcgctgag gtctcgcaac tgtgtgatgt tctcctctac gatcggcagc agcgcgacat tgtgttggtg ttcgcgtacg ccacagcgag tctggctggc ctgtgtcgcc gccagttcgc cttcacacca tcggagatgg cccttctcat gaagaagctt ctcatcgcgg tgcgcaagct gcacgagatg cgcatcattc accgggacat caagtctgac aacgtactcg taacgagtga gggcgaggtg cagctgaccg actttgggct gtgctccatt gcagcgcctg ggtcgtcgcg atgtggcacg catgtgtggc ggacgccgag cgtgattact ctggcctacc gtccgccaga gatgctgttg ggcagtaccg cgtatgatga gaaggtcgat gtgtggtcgc ttgggtgcct gctcgctcag atgttcctcc tcgagccccc cttctacagg caccgcgcgc aggcgcagca gcagcagcaa cgtgctccag agcgatcggc ggccacggag ctcgagcagc tctcccgcat tacagagatt ctggggccac tgccgccggt gagcgtttat cacccggact cgtgtcagca catgcgggtt ctagagcagc tggaagtgca ggggcgcctg gcggaggctg ggcgagcagc acaaccggca aactggggca ggctgcagac catcttcgag cccagctttc tgtatcagca gttccacggc tttcggggct ggtttgaggc ggagttgggg cgctcgcggc accagccaca tcgtcggccc acgcaggctt gcatggatgt gctctgcgcg gccctgcaac tcgacccgca gcagcgtccc accgcggcag agctgctgcg catgccgtac tttaccacct tggacgacgc cccgttgctg ggcagctatc aacgggtcct ccccgtcacc ccggagcggg aagctgaggt acgccgcggt ttcatgacaa aggtgcaacg atgcggggac agtcacacgc agcgccgccc gcatcagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at