LedoA.00425.a

Elongation factor 1-alpha

CENTER ID: LedoA.00425.a
ORGANISM: Leishmania donovani
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LedoA.00425.a.F11.PS01005 full length 1 449
External Resources
RESOURCE REFERENCE ID
EuPathDB: TritrypDB:LdBPK_170170.1
OrthoMCL: OG5_126631
RefSeq: XP_003859873.1
UniProt: Q95VF2
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKDKVHMNL VVVGHVDAGK STATGHLIYK CGGIDKRTIE KFEKEAAEIG KASFKYAWVL DKLKAERERG ITIDIALWKF ESPKSVFTII DAPGHRDFIK NMITGTSQAD AAILMIDSTH GGFEAGISKD GQTREHALLA FTLGVKQMVV CCNKMDDKTV TYAQSRYDEI SKEVGAYLKR VGYNPEKVRF IPISGWQGDN MIERSDNMPW YKGPTLLDAL DMLEPPVRPV DKPLRLPLQD VYKIGGIGTV PVGRVETGIM KPGDVVTFAP ANVTTEVKSI EMHHEQLAEA QPGDNVGFNV KNVSVKDIRR GNVCGNSKND PPKEAADFTA QVIVLNHPGQ ISNGYAPVLD CHTSHIACRF AEIESKIDRR SGKELEKNPK AIKSGDAAIV KMVPQKPMCV EVFNDYAPLG RFAVRDMRQT VAVGIIKGVN KKEGSGGKVT KAAAKAAKK
NT Sequence
atgggcaagg ataaggtgca catgaacctt gtggtcgtcg gccatgtcga cgccggcaag tccaccgcca ctggccactt gatctacaag tgcggtggca tcgacaagcg cacgatcgag aagttcgaga aggaggccgc cgagatcggc aaggcgtcct tcaagtacgc gtgggtgctc gacaagctga aggcggagcg cgagcgcggc atcacgatcg acattgcgct gtggaagttc gagtcgccca agtccgtgtt cacgatcatc gatgcgcccg gccaccgcga cttcatcaag aacatgatca cgggcacgtc gcaggcggac gccgccatcc tgatgatcga ctcgacgcat ggtggcttcg aggctggcat ctcgaaggac ggccagaccc gcgagcacgc gctgcttgcc ttcacgcttg gcgtgaagca gatggtggtg tgctgcaaca agatggacga caagaccgtg acgtacgcgc agtcgcgcta cgatgagatc agcaaggagg tgggcgcgta cctgaagcgc gtgggctaca accccgagaa ggtgcgcttc atcccgatct cgggctggca gggcgacaac atgatcgaga ggtcggacaa catgccgtgg tacaagggtc ccacgctgct ggacgcgctc gacatgctgg agccgccggt gcgcccggtg gacaagccgc tgcgcctgcc cctgcaggac gtgtacaaga tcggcggtat cgggactgtg cccgtgggcc gcgtggagac cggcatcatg aagccgggcg acgtggtgac gttcgcgccc gccaacgtga cgactgaggt gaagtcgatc gagatgcacc acgagcagct ggcggaggcg cagcccggcg acaacgtcgg cttcaacgtg aagaacgtgt cggtgaagga catccgccgt ggcaacgtgt gcggcaactc gaagaacgac ccgccgaagg aggcggccga cttcacggcg caggtgatcg tgctgaacca ccccggccag atcagcaacg gctacgcgcc ggtgctggac tgccacacga gccacattgc gtgccgcttc gcggaaatcg agtccaagat cgaccgccgc tccggcaagg agctggagaa gaaccccaag gcgatcaagt ctggcgatgc cgcgatcgtg aagatggtgc cgcagaagcc gatgtgcgtg gaggtgttca acgactacgc gccgctgggc cgctttgccg tgcgcgacat gcggcagacg gtggccgtgg gcatcatcaa gggcgtgaac aagaaggagg gcagcggcgg taaggtgacc aaggcggccg cgaaggctgc gaagaag
Details for LedoA.00425.a.F11.PS01005
PURIFICATION DATe: 2/25/2011
CONCENTRATION: 13.71mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 50µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG KDKVHMNLVV VGHVDAGKST ATGHLTYKCG GIDKRTIEKF EKEAAEIGKA SFKYAWVLDK LKAERERGIT IDIALWKFES PKSVFTIIDA PGHRDFIKNM ITGTSQADAA ILMIDSTHGG FEAGISKDGQ TREHALLAFT LGVKQMVVCC NKMDDKTVTY AQSRYDEISK EVGAYLKRVG YNPEXVRFIP ISGWQGDNMI ERSDNMPWYK GPTLLDALDM LEPXVRPVDK PLRLPLQDVY KIGGIGTVPV GRVETGIMKP GDVVTFAPAN VTTEVKSIEM HHEQLAEAQP GDNVGFNVKN VSVKDIRRGN VCGNSKNDPP KEAADFTAQV IVLNHPGQIS NGYAPVLDCH TSHIACRFAE IESKIDRRSG KELEKNPKAI KSGDAAIVKM VPQKPMCVEV FNDYAPLGRF AVRDMRQTVA VGIIKGVNKK EGSGGKVTKA AAKAAKK
Validated NT Sequence
atggctcacc accaccacca ccatatgggc aaggataagg tgcacatgaa ccttgtggtc gtcggccatg tcgacgccgg caagtccacc gccactggcc acttgaccta caagtgcggt ggcatcgaca agcgcacgat cgagaagttc gagaaggagg ccgccgagat cggcaaggcg tccttcaagt acgcgtgggt gctcgacaag ctgaaggcgg agcgcgagcg cggcatcacg atcgacattg cgctgtggaa gttcgagtcg cccaagtccg tgttcacgat catcgatgcg cccggccacc gcgacttcat caagaacatg atcacgggca cgtcgcaggc ggacgccgcc atcctgatga tcgactcgac gcatggtggc ttcgaggctg gcatctcgaa ggacggccag acccgcgagc acgcgctgct tgccttcacg cttggcgtga agcagatggt ggtgtgctgc aacaagatgg acgacaagac cgtgacgtac gcgcagtcgc gctacgatga gatcagcaag gaggtgggcg cgtacctgaa gcgcgtgggc tacaacccgg agaangtgcg cttcatcccg atctcgggct ggcagggcga caacatgatc gagaggtcgg acaacatgcc gtggtacaag ggtcccacgc tgctggacgc gctcgacatg ctggagccgc cngtgcgccc ggtggacaag ccgctgcgcc tgcccctgca ggacgtgtac aagatcggcg gtatcgggac tgtgcccgtg ggccgcgtgg agaccggcat catgaagccg ggcgacgtgg tgacgttcgc gcccgccaac gtgacgactg aggtgaagtc gatcgagatg caccacgagc agctggcgga ggcgcagccc ggcgacaacg tcggcttcaa cgtgaagaac gtgtcggtga aggacatccg ccgtggcaac gtgtgcggca actcgaagaa cgacccgccg aaggaggcgg ccgacttcac ggcgcaggtg atcgtgctga accaccccgg ccagatcagc aacggctacg cgccggtgct ggactgccac acgagccaca ttgcgtgccg cttcgcggaa atcgagtcca agatcgaccg ccgctccggc aaggagctgg agaagaaccc caaggcgatc aagtctggcg atgccgcgat cgtgaagatg gtgccgcaga agccgatgtg cgtggaggtg ttcaacgact acgcgccgct gggccgcttt gccgtgcgcg acatgcggca gacggtggcc gtgggcatca tcaagggcgt gaacaagaag gagggcagcg gcggtaaggt gaccaaggcg gccgcgaagg ctgcgaagaa gtaa
Expressed Protein Sequence
MAHHHHHHMM GKDKVHMNLV VVGHVDAGKS TATGHLIYKC GGIDKRTIEK FEKEAAEIGK ASFKYAWVLD KLKAERERGI TIDIALWKFE SPKSVFTIID APGHRDFIKN MITGTSQADA AILMIDSTHG GFEAGISKDG QTREHALLAF TLGVKQMVVC CNKMDDKTVT YAQSRYDEIS KEVGAYLKRV GYNPEKVRFI PISGWQGDNM IERSDNMPWY KGPTLLDALD MLEPPVRPVD KPLRLPLQDV YKIGGIGTVP VGRVETGIMK PGDVVTFAPA NVTTEVKSIE MHHEQLAEAQ PGDNVGFNVK NVSVKDIRRG NVCGNSKNDP PKEAADFTAQ VIVLNHPGQI SNGYAPVLDC HTSHIACRFA EIESKIDRRS GKELEKNPKA IKSGDAAIVK MVPQKPMCVE VFNDYAPLGR FAVRDMRQTV AVGIIKGVNK KEGSGGKVTK AAAKAAKK
Full NT Sequence (Expression Vector + Insert)
GAACTCACCT ATCTCCCCAA CACCTAATAA CATTCAATCA CTCTTTCCAC TAACCACCTA TCTACATCAC CAAGATATCA CTAGTTCTCG AGATGGCTCA CCACCACCAC CACCATATGA TGGGCAAGGA TAAGGTGCAC ATGAACCTTG TGGTCGTCGG CCATGTCGAC GCCGGCAAGT CCACCGCCAC TGGCCACTTG ATCTACAAGT GCGGTGGCAT CGACAAGCGC ACGATCGAGA AGTTCGAGAA GGAGGCCGCC GAGATCGGCA AGGCGTCCTT CAAGTACGCG TGGGTGCTCG ACAAGCTGAA GGCGGAGCGC GAGCGCGGCA TCACGATCGA CATTGCGCTG TGGAAGTTCG AGTCGCCCAA GTCCGTGTTC ACGATCATCG ATGCGCCCGG CCACCGCGAC TTCATCAAGA ACATGATCAC GGGCACGTCG CAGGCGGACG CCGCCATCCT GATGATCGAC TCGACGCATG GTGGCTTCGA GGCTGGCATC TCGAAGGACG GCCAGACCCG CGAGCACGCG CTGCTTGCCT TCACGCTTGG CGTGAAGCAG ATGGTGGTGT GCTGCAACAA GATGGACGAC AAGACCGTGA CGTACGCGCA GTCGCGCTAC GATGAGATCA GCAAGGAGGT GGGCGCGTAC CTGAAGCGCG TGGGCTACAA CCCCGAGAAG GTGCGCTTCA TCCCGATCTC GGGCTGGCAG GGCGACAACA TGATCGAGAG GTCGGACAAC ATGCCGTGGT ACAAGGGTCC CACGCTGCTG GACGCGCTCG ACATGCTGGA GCCGCCGGTG CGCCCGGTGG ACAAGCCGCT GCGCCTGCCC CTGCAGGACG TGTACAAGAT CGGCGGTATC GGGACTGTGC CCGTGGGCCG CGTGGAGACC GGCATCATGA AGCCGGGCGA CGTGGTGACG TTCGCGCCCG CCAACGTGAC GACTGAGGTG AAGTCGATCG AGATGCACCA CGAGCAGCTG GCGGAGGCGC AGCCCGGCGA CAACGTCGGC TTCAACGTGA AGAACGTGTC GGTGAAGGAC ATCCGCCGTG GCAACGTGTG CGGCAACTCG AAGAACGACC CGCCGAAGGA GGCGGCCGAC TTCACGGCGC AGGTGATCGT GCTGAACCAC CCCGGCCAGA TCAGCAACGG CTACGCGCCG GTGCTGGACT GCCACACGAG CCACATTGCG TGCCGCTTCG CGGAAATCGA GTCCAAGATC GACCGCCGCT CCGGCAAGGA GCTGGAGAAG AACCCCAAGG CGATCAAGTC TGGCGATGCC GCGATCGTGA AGATGGTGCC GCAGAAGCCG ATGTGCGTGG AGGTGTTCAA CGACTACGCG CCGCTGGGCC GCTTTGCCGT GCGCGACATG CGGCAGACGG TGGCCGTGGG CATCATCAAG GGCGTGAACA AGAAGGAGGG CAGCGGCGGT AAGGTGACCA AGGCGGCCGC GAAGGCTGCG AAGAAGGTGA GTAAGATAGG ATCCATATAT AGGGCCCGGG TTATAATTAC CTCAGGTCGA CGTCCCATGG TTTTGTATAG AATTTACGGC TAGCGCCGGA TGCGACGCCG GTCGCGTCTT ATCCGGCCTT CCTATATCAG GCGGTGTTTA AGACGCCGCC GCTTCGCCCA AATCCTTATG CCGGTTCGAC GACTGGACAA AATACTGTTT ATCTTCCCAG CGCAGGCAGG TTAATGTACC ACCCCAGCAG CAGCCGGTAT CCAGCGCGTA TATACCTTCC GGCGTACCTT TGCCCTCCAG CGATGCCCAG TGACCAAAGG CGATGCTGTA TTCTTCAGCG ACAGGGCCAG GAATCGCAAA CCACGGTTTC AGTGGGGCAG GGGCCTCTTC CGGCGATTCT TACTAGCTAG TATGCATAGG TGCTGAAATA TAAAGTTTGT GTTTCTAAAA CACACTTGGT ACGTACGATA ACGTACAGTG TTTTTCCCTC CACTTAAATC GAAGGGTAGT GTCTTGGAGC GCGCGGAGTA AACATATATG GTTCATATAT GTCCGTAGGC ACGTAAAAAA AGCGAGGGAT TCGAATTCCC CCGGAACCCC CGGTTGGGGC CCACGCCTCG ATCGAGCAAA AAAAAAAAAA AAGAAAAAAA AAAAAAAAAA AAGCTTTCCC GCGGCCAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCACT CACTGACTCG CTGCGCTCGG TCGCTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGCGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC TGGCTAGCGA TGACCCTGCT GATTGGTTCG CTGACCATTT CCGGGTGCGG GACGGCGTTA CCAGAAACTC AGAAGGTTCG TCCAACCAAA CCGACTCTGG CGGCAGTTTA CGAGAGAGAT GATAGGGTCT GCTTCAGTAA GCCAGATGCT ACACAATTAG GCTTGTACAT ACTGTCGTTA GAACGCGGCT ACAATTAATA CATAACCTTA TGTATCATAC ACATACGATT TAGGTGACAC TATA