LebrA.20384.a

Protein kinase domain-containing protein

CENTER ID: LebrA.20384.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.20384.a.HZ2.GE43708 kinase domain 1 509
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.21.0330
UniProt: A4HBM5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATVDTVKGR YSSYKLLNEV GRGGSALVYR AQDVATHKDV AVKQLFGNRP QDIEDWLREV DALHSLNSHH CPHVVKYVDH MQQHDRLFLV EEFTEHGSLL RRLKKKGRFA EDIVCQYVYQ VLTALYHMAP WGVVHGDLKA SNILLFDGDV VKLNDFALRS RAEDEHEDAE RHITASSTSS AEASSLTRGS RAGRSTSEEG AGIALSGFRG SAYWAAPEVL SGASKATAAS DIWSVGCLVV ELLTGTPPYF ERTIHNAIYH ILKGYYEVVG ESEPGRCGAG GTSRAAARSA APSATTGPGD DDGPRGPRKK KNGVKDASGA GLPKVDAASA RKTTAATNTP SPAKEVADAA RTAASGGTAS AVDRHEVAAS LCEVTLLPPL PKDLHVSNEC MSFLRMCFRP RAADRPSAGE LLHEPWFLNC AVPQLLRAAR EGRPMNGTTG DEIIAGSSGS RFGVIEQWVE RNLACDDESR CEAWLNSDAL PLLVPVLTPR IMTPKYIGDV MGRFSHFAEG GSSLATLFLN RLGSTDLWGV EELTSACDAD HLATLFRRCC ATQDAQVSVY TPTDPRALRF VLGLEKEKVL ACVRALHSRL VLEPAAVAVT TTARSPTAVT DDAAVDLNDR SPTSCGPAAP AHVTPACAPA SLQEQHEEQQ YACERLLSDG GAAVLCQCVE AQCKAAFLSN TAPMMGWDTI NLLVDVLSTI EPLAGGKMLL WGIRSGHSSL NATGGAGSGS GASVVRSLTV AATPRGGRGG EMNSTWDKSS PATGAPSLSS PLLVNPQGSV SAAGSGGQLN SLSSTMGLGH RTVSGLSPEP VEWITSVSWL LAVQEAARHL CEAAVRLLTR YIPVARECRA EHLEKAGVSL TATLVLVASS EVVSTEVRCA AVEALPQLQA SSLRAARYLR DPVRCIALLA LTLKRSYGVP ALTSCLLTAI TTMTTEKQML AACTSCPWVW EALVSLLGEV EAADNTVAAD CNRSQSVSHR VANTNVGPSE EGDAPSALPV VLASPTAVKS PSASSSSSNS SSASAAFADI IVVLLRWFAE VTPTALLSSA AAAAAAASGA LPALGHSAAP PPLSVLLQSL RRQLIALSQN SLVCHGDLIP NVAKVLRHLT PLEAAAVAAS TPTVTVA
NT Sequence
atggccaccg tagacaccgt caaagggcgg tactcgtcct acaagctgct caacgaggtc ggccgcggtg gcagcgcact cgtataccgt gcgcaagacg tggccacaca caaggatgtt gccgtgaagc agctcttcgg caaccggccg caggacatag aggactggct acgcgaggtg gacgcccttc actcacttaa cagccatcac tgcccgcacg tggtgaagta cgtggaccac atgcagcaac acgatcgact cttcctcgtg gaagagttca ccgagcacgg gtcgctgctg cgacgtctga agaagaaagg cagatttgcg gaggacattg tttgccaata cgtctatcag gtgctgaccg ccctgtacca catggccccg tggggcgtcg tacacggcga cctgaaggcg tccaacatcc ttctcttcga tggggatgtt gtgaagctga acgactttgc actacgctcg cgtgccgagg acgagcacga ggatgcagag cggcacatca ctgcttcctc cacgtcgtct gccgaagcat cgtccctcac gaggggcagc cgcgctggga gaagcactag tgaagagggt gctggcatcg ccctctcggg gttccgtggc tccgcctact gggcagcgcc ggaggtcctc agcggcgcct ctaaggcaac ggcggcgagc gacatctggt cggtgggctg tctcgtggtg gagctgctga cgggcacccc gccgtacttc gaacgcacca tccacaacgc catctatcac atcctcaagg gctattacga ggtggtcggc gaaagcgagc ccggccgttg cggtgctggc ggcacctcgc gcgccgcggc ccgctccgcg gccccaagcg ccacaacggg ccctggcgac gatgacggac cgcgcgggcc gcggaagaag aagaacggag tgaaggatgc gagtggtgcg gggttaccaa aggtggatgc tgcgtccgcg aggaagacga cggccgcgac gaacactccc agtccggcga aggaggtggc ggatgcggcc aggacagcgg catctggggg cactgcaagt gcagtagacc gacatgaagt ggccgcatct ctctgtgagg tgacacttct cccaccgctt cccaaggatc tgcacgtcag caacgagtgt atgtcgtttc tacggatgtg cttccgcccc cgcgcggcgg accggccgtc ggctggagag ctgctccatg agccgtggtt cctgaactgc gcggtgccgc agcttctgcg agcggcgcgg gaaggtcggc ctatgaacgg aaccacaggg gacgagatca tcgccggcag cagcggcagc cgctttgggg tgatcgagca gtgggtggaa cgcaacctag cttgtgatga cgagagtcgg tgcgaggcgt ggctgaacag cgacgcgctg ccgctgctgg tcccggtgtt gaccccgcgt attatgacac ccaaatacat tggagacgta atggggcgct tttcccactt tgccgagggt ggttcatcct tggcgacgct ctttctgaac cggctggggt cgaccgatct ctggggggtg gaggagttga cgtcggcgtg cgacgcggac cacctcgcca cactcttccg gcggtgctgt gcgacgcaag atgcacaggt gtccgtgtac actccgacag acccgcgtgc actgcgcttc gtcttgggcc tggagaagga aaaggtcctg gcatgtgtgc gcgcgctgca ctcgcgcctc gtgctggagc cggcggctgt ggcggtgact acgactgcga gatcgccgac cgccgtcacg gacgatgccg cagttgatct gaacgaccgc tcccccacct cgtgtggccc cgcagcgcct gcgcatgtga ctccggcttg cgcgccggca tctcttcaag aacagcacga ggaacaacag tatgcctgtg aacggctact cagcgacggc ggcgcggcgg tgctgtgcca gtgcgtcgag gcacagtgca aggctgcctt cttgagtaac acagcgccga tgatggggtg ggacacgatt aaccttttgg ttgacgtgct ctccaccatc gagccactgg cgggtgggaa gatgctgctg tgggggatca ggagtggcca cagctccctg aacgccactg gaggcgcagg tagtggtagt ggagcttcgg ttgttagatc actgacggtg gcggcaaccc cacgcggagg acgaggtggc gagatgaaca gcacctggga caagtcatcg ccagcgacgg gtgccccatc tttgtcgtca ccgctcttgg tgaacccaca ggggtcagtg tcggcggcag gcagtggcgg ccagctgaac tccctaagca gcaccatggg cttggggcac cgcactgtca gtgggctttc accagagcca gtggagtgga tcacctccgt gtcgtggctg ctcgcagtgc aggaagcggc gcggcacctg tgcgaggcag cggtgcgcct gcttacccgc tacattccag tcgccagaga gtgcagagcc gagcatctcg agaaggctgg tgtcagccta acggcaacgc tggtcctcgt agcctcgagc gaggtggtca gcaccgaggt gcgatgcgca gccgtcgaag ctctgccaca actgcaggcg agctctctcc gtgcggctcg gtacctgcgc gacccagtgc gatgtattgc cctgctggcc ctcaccctga agaggtctta cggtgtccct gccctgacat cgtgtctgct cacagccatc accaccatga cgaccgagaa gcagatgcta gcggcctgca ccagctgccc gtgggtgtgg gaggcactag tgtccttgtt gggggaggtg gaagcagccg acaataccgt tgccgccgat tgcaaccgta gccaatcggt ctcccacaga gtcgctaaca ccaacgttgg cccgtccgag gagggtgacg ccccgtctgc tttaccagta gtattggcaa gccctacagc agtcaaatca cctagcgcaa gcagcagcag cagcaacagc agcagcgctt ccgctgcctt tgccgacatt atcgttgttc tcttgcgttg gttcgcagag gtgacgccga cagcgctgct gagcagtgca gcagcagcag cggcagcggc atcgggggca ttgcctgccc tcggtcattc agccgcgccc ccacccctct ctgtgttgct tcagtcactt cgccgtcagc tcatagcgct gtcgcagaac agtcttgtct gtcacggtga tctcataccg aatgtagcaa aggtgctgcg gcatttgact cctctcgaag ccgccgccgt cgccgcgtca acgccgacag tgacggtggc atga
Details for LebrA.20384.a.HZ2.GE43708
HARVESTED ON: data unavailable
SEQUENCED ON: 6/23/2020
EXPECTED MW: 68kDa
OBSERVED MW: 68kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMA TVDTVKGRYS SYKLLNEVGR GGSALVYRAQ DVATHKDVAV KQLFGNRPQD IEDWLREVDA LHSLNSHHCP HVVKYVDHMQ QHDRLFLVEE FTEHGSLLRR LKKKGRFAED IVCQYVYQVL TALYHMAPWG VVHGDLKASN ILLFDGDVVK LNDFALRSRA EDEHEDAERH ITASSTSSAE ASSLTRGSRA GRSTSEEGAG IALSGFRGSA YWAAPEVLSG ASKATAASDI WSVGCLVVEL LTGTPPYFER TIHNAIYHIL KGYYEVVGES EPGRCGAGGT SRAAARSAAP SATTGPGDDD GPRGPRKKKN GVKDASGAGL PKVDAASARK TTAATNTPSP AKEVADAART AASGGTASAV DRHEVAASLC EVTLLPPLPK DLHVSNECMS FLRMCFRPRA ADRPSAGELL HEPWFLNCAV PQLLRAAREG RPMNGTTGDE IIAGSSGSRF GVIEQWVERN LACDDESRCE AWLNSDALPL LVPVLTPRIM TPKYIGDVMG RFSHFAE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tggccaccgt agacaccgtc aaagggcggt actcgtccta caagctgctc aacgaggtcg gccgcggtgg cagcgcactc gtataccgtg cgcaagacgt ggccacacac aaggatgttg ccgtgaagca gctcttcggc aaccggccgc aggacataga ggactggcta cgcgaggtgg acgcccttca ctcacttaac agccatcact gcccgcacgt ggtgaagtac gtggaccaca tgcagcaaca cgatcgactc ttcctcgtgg aagagttcac cgagcacggg tcgctgctgc gacgtctgaa gaagaaaggc agatttgcgg aggacattgt ttgccaatac gtctatcagg tgctgaccgc cctgtaccac atggccccgt ggggcgtcgt acacggcgac ctgaaggcgt ccaacatcct tctcttcgat ggggatgttg tgaagctgaa cgactttgca ctacgctcgc gtgccgagga cgagcacgag gatgcagagc ggcacatcac tgcttcctcc acgtcgtctg ccgaagcatc gtccctcacg aggggcagcc gcgctgggag aagcactagt gaagagggtg ctggcatcgc cctctcgggg ttccgtggct ccgcctactg ggcagcgccg gaggtcctca gcggcgcctc taaggcaacg gcggcgagcg acatctggtc ggtgggctgt ctcgtggtgg agctgctgac gggcaccccg ccgtacttcg aacgcaccat ccacaacgcc atctatcaca tcctcaaggg ctattacgag gtggtcggcg aaagcgagcc cggccgttgc ggtgctggcg gcacctcgcg cgccgcggcc cgctccgcgg ccccaagcgc cacaacgggc cctggcgacg atgacggacc gcgcgggccg cggaagaaga agaacggagt gaaggatgcg agtggtgcgg ggttaccaaa ggtggatgct gcgtccgcga ggaagacgac ggccgcgacg aacactccca gtccggcgaa ggaggtggcg gatgcggcca ggacagcggc atctgggggc actgcaagtg cagtagaccg acatgaagtg gccgcatctc tctgtgaggt gacacttctc ccaccgcttc ccaaggatct gcacgtcagc aacgagtgta tgtcgtttct acggatgtgc ttccgccccc gcgcggcgga ccggccgtcg gctggagagc tgctccatga gccgtggttc ctgaactgcg cggtgccgca gcttctgcga gcggcgcggg aaggtcggcc tatgaacgga accacagggg acgagatcat cgccggcagc agcggcagcc gctttggggt gatcgagcag tgggtggaac gcaacctagc ttgtgatgac gagagtcggt gcgaggcgtg gctgaacagc gacgcgctgc cgctgctggt cccggtgttg accccgcgta ttatgacacc caaatacatt ggagacgtaa tggggcgctt ttcccacttt gccgagtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa