LebrA.20382.a

Putative serine/threonine-protein kinase (EC 2.7.11.1)

CENTER ID: LebrA.20382.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.20382.a.HZ2.GE43707 kinase domain 119 565
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.30.3610
UniProt: A4HIU4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKSKKHGKP AKGVLKRGNK NDKCKRKAKF IDPASSVSRD VRESVEALVE EIEREELVVE PQPLIKAKKK RQELVEKESV LFKSGMTKEQ GHTITNKLQQ QGTTHRSEGE GDEESNDGDG GGTEEDYSDT ANEPSREYRK GGYHHVVIGE VYNNRYRVVK KLGWGYFSTV WLVWDYHKER YQAMKIQKSA ASYSEAAYDE IKLLTEIMEA DPHKTRCCAR LNDYFKHTGP NGTHVCMLFD VYGENLLSLM ERYEYRGIPL PIVKCIARQV LIGLDHINSI DIIHTDLKPE NVLLSTPKHS IISLMKHFHP PPLHQRPKLT ERDPKTMTKS QRRRYYKKLA KEERKTLLCE KDGEHTSRGD ERGTNENGDT DSELSKTDPE WEVERFHHVI LADFGNSCWT YKQFTDEVQT RQYRCPEVIL GESYSTSIDI WSCACMIFEL ITGQFLFDPK KGDDYSRDED HLALMSELLG DLPESMRLGD GKYRSHYYNS KGVLRNIKDL QYWILEDVLH QRHKFTKKKA KEIADFLLPM LKYSPDTRAT PAAMLRDHDA FFDIQEDDYA PLCFVDEGGD DEEGSASDDE EETDSDSSLD SYSSSYSSER KANRSRQLNN GADRSEAFRY WEEHPILNRT YLEERGLTIA DVQSVLAGNF LDDAATHKAA AEVIRLLSEE ADRLSSDRGS AGDEADNEDE DDSSDDDKES TQDSEKPVEV AVGGVACSNH GVEEGKADST DKEDEESDDG TD
NT Sequence
atggggaagt caaagaagca cggtaaacca gcaaaaggtg tattgaagag gggaaacaag aatgacaagt gcaagcgcaa ggctaagttc atagatcctg caagcagcgt aagtcgcgat gtgcgtgaaa gcgtggaggc cctcgttgaa gaaatcgagc gcgaggagct ggtggtggag ccgcagcccc ttatcaaggc aaagaagaag cgacaggagc tggtcgaaaa ggagagcgtc ctctttaaaa gcggcatgac aaaggagcag gggcacacga tcaccaacaa actccagcag cagggcacaa cgcaccgtag tgagggcgag ggcgacgaag agagcaacga cggcgacggc ggtggcactg aggaagacta cagtgacacg gcaaacgagc cctctcgtga gtaccgcaag ggcggctacc accacgtagt cattggtgaa gtctacaaca accgctaccg cgtcgtcaag aagctaggct ggggctactt ctctacggtg tggctggtgt gggactacca caaggagcgt taccaggcaa tgaagattca gaaatcggcg gcaagctaca gcgaggccgc ctacgatgag atcaaactgc tcacggagat tatggaagca gacccgcaca agactcgttg ttgtgctcgc ttgaacgact acttcaagca cacggggccg aacgggacgc acgtttgcat gctctttgac gtgtacggcg agaacttgct ctccctgatg gagcgctacg agtaccgcgg tattcctcta cccattgtca agtgcattgc gcggcaggtg ctcattgggc ttgaccacat caactctatt gacatcatcc acacagacct gaagccagag aatgtactct tatccacccc gaagcactct ataatatcgc tgatgaagca ctttcaccct cctcccctgc atcagcgccc aaagcttacg gagcgcgacc ccaagaccat gacaaagtca cagcgaaggc gctactacaa gaagctggca aaggaggaac gaaagacgct gctgtgcgag aaggatggcg agcatacatc tagaggcgac gagcgcggca cgaacgagaa cggggatacg gattcagagt tgagcaagac ggatccggag tgggaggtcg agcgttttca ccacgtcatc ctcgccgact ttggtaatag ctgctggaca tacaaacaat tcacagacga ggtgcagaca cggcagtatc gctgcccgga ggtcattcta ggcgagtcgt actctacttc aatcgacatt tggtcttgcg cctgcatgat tttcgagctc attacgggtc agttcctttt cgacccaaag aagggcgatg actactcgcg cgatgaggat cacctggctc tgatgagcga gcttctcggt gatctgccgg agagcatgcg actgggcgac ggcaagtacc gaagccacta ctacaactcc aaaggagtct tgcgcaacat taaagatctg cagtactgga tcctagagga tgtactgcat cagcggcaca agttcacgaa aaaaaaggca aaggagatcg ccgacttcct gctacccatg ctcaagtact cccccgacac ccgcgccacc cccgccgcga tgctgcgcga tcacgacgcc ttctttgata tccaggagga tgactacgca ccgctgtgct ttgttgatga ggggggtgac gacgaagaag gctctgcttc ggacgacgaa gaggaaactg acagtgatag ctctttggat tcctactcgt cctcctattc cagcgaacgg aaagcgaatc gctcccggca gctgaacaac ggtgccgacc gctctgaggc cttccggtac tgggaggagc atccgattct gaaccgcacc tacctagagg aacgagggtt gaccattgct gacgttcagt cagttctcgc cgggaacttc ttagacgacg ccgcaaccca taaggctgcc gcggaggtga ttcgtctgct ctcagaggag gcggaccgtc tctcgagcga ccgtggcagc gctggagatg aggcggataa tgaggacgag gacgacagca gcgatgatga caaggaatcc acgcaggact ctgagaagcc agttgaggtg gctgtgggtg gcgtggcttg tagcaaccat ggcgttgaag aaggcaaggc ggactctaca gataaagaag atgaagagag tgacgacggc actgactag
Details for LebrA.20382.a.HZ2.GE43707
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 66kDa
OBSERVED MW: 66kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSDG GGTEEDYSDT ANEPSREYRK GGYHHVVIGE VYNNRYRVVK KLGWGYFSTV WLVWDYHKER YQAMKIQKSA ASYSEAAYDE IKLLTEIMEA DPHKTRCCAR LNDYFKHTGP NGTHVCMLFD VYGENLLSLM ERYEYRGIPL PIVKCIARQV LIGLDHINSI DIIHTDLKPE NVLLSTPKHS IISLMKHFHP PPLHQRPKLT ERDPKTMTKS QRRRYYKKLA KEERKTLLCE KDGEHTSRGD ERGTNENGDT DSELSKTDPE WEVERFHHVI LADFGNSCWT YKQFTDEVQT RQYRCPEVIL GESYSTSIDI WSCACMIFEL ITGQFLFDPK KGDDYSRDED HLALMSELLG DLPESMRLGD GKYRSHYYNS KGVLRNIKDL QYWILEDVLH QRHKFTKKKA KEIADFLLPM LKYSPDTRAT PAAMLRDHDA FFDIQEDDYA PLCFV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcgg acggcggtgg cactgaggaa gactacagtg acacggcaaa cgagccctct cgtgagtacc gcaagggcgg ctaccaccac gtagtcattg gtgaagtcta caacaaccgc taccgcgtcg tcaagaagct aggctggggc tacttctcta cggtgtggct ggtgtgggac taccacaagg agcgttacca ggcaatgaag attcagaaat cggcggcaag ctacagcgag gccgcctacg atgagatcaa actgctcacg gagattatgg aagcagaccc gcacaagact cgttgttgtg ctcgcttgaa cgactacttc aagcacacgg ggccgaacgg gacgcacgtt tgcatgctct ttgacgtgta cggcgagaac ttgctctccc tgatggagcg ctacgagtac cgcggtattc ctctacccat tgtcaagtgc attgcgcggc aggtgctcat tgggcttgac cacatcaact ctattgacat catccacaca gacctgaagc cagagaatgt actcttatcc accccgaagc actctataat atcgctgatg aagcactttc accctcctcc cctgcatcag cgcccaaagc ttacggagcg cgaccccaag accatgacaa agtcacagcg aaggcgctac tacaagaagc tggcaaagga ggaacgaaag acgctgctgt gcgagaagga tggcgagcat acatctagag gcgacgagcg cggcacgaac gagaacgggg atacggattc agagttgagc aagacggatc cggagtggga ggtcgagcgt tttcaccacg tcatcctcgc cgactttggt aatagctgct ggacatacaa acaattcaca gacgaggtgc agacacggca gtatcgctgc ccggaggtca ttctaggcga gtcgtactct acttcaatcg acatttggtc ttgcgcctgc atgattttcg agctcattac gggtcagttc cttttcgacc caaagaaggg cgatgactac tcgcgcgatg aggatcacct ggctctgatg agcgagcttc tcggtgatct gccggagagc atgcgactgg gcgacggcaa gtaccgaagc cactactaca actccaaagg agtcttgcgc aacattaaag atctgcagta ctggatccta gaggatgtac tgcatcagcg gcacaagttc acgaaaaaaa aggcaaagga gatcgccgac ttcctgctac ccatgctcaa gtactccccc gacacccgcg ccacccccgc cgcgatgctg cgcgatcacg acgccttctt tgatatccag gaggatgact acgcaccgct gtgctttgtt taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa