LebrA.20227.a

protein kinase, putative (OPH8)

CENTER ID: LebrA.20227.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.20227.a.B2.GE42790 PHoranyi kinase domain truncation 441 744
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.25.2570
UniProt: A4HEK0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNPSAKRHAY ELLLPQQQEP TNPLSAHQSC RGSTGASGEN TTLPQLSSNS VPATIHITEP SANSTLMFHS AAASTLVPIL HGRTGAAAAA RDVNDGNAGM HPRPRRRVSQ SYPGALTSAV PSMALAVVPF TGSDGVGISS PQRGDSRVGG AGQDGNNTHG SGDSVGYYSG PSPYAKLAPL SLSRERVCPH CLRSYDVFDD SEGVFDQATF FHVDATTAPS SLRPSSHRKL LPPPSQEGDE QQQQQPSASS RLYTDNAGDG GNATGQLLYN GCSTASGFDE GSSNGTNRSG NYIAALRPPA AAFTSHYFRA LPPPSFITAV GAHNASTLLA IEDYRPSLLH DSPSGEEEAR QPCTVAPGPA SGEEGRDSSG TMVCKAVAVP HYSYVSPTTC TGMRSQVRLL QQHDVKKTAT EVEHENEEWL SCESDSLVYS PIAAEPSPNN GYYRHYFKEL RQLGRGTYGG VYLCRHIMCG VNLGEFALKK IPVGDKATYL QSVLREVRIL EEVRRHPNVV EYKHSWVEEA QLADFGPPVR CLFILMEYAS AGSLDTYLER YGNHLSTLAV WYFFLSSVAG TAHLHEKHIL HRDLKPQNLL LAAMEGRPPR VLVSDFGTAA LLGDISYDRS GGTGTLEYMA PELLETAASP HGINERYVNH HTMASDVWSL GMVLHYLAFD GTLPERREDG SVNLDEAHRS ANDRPPELLK LLEAMLQLDP ARRPRCSDIL GSPMVQSIMR IFNKDDHSQW DLSIQEQQRL ATAPSAILPL ERPSRPPVSV PPPRSAPLKL PEPSSIDVRN SPPSARSRNS ITAISNSSPG RGMRADRTNA KDKSNALHSS RVELLSASAL GDTSTVAARG GSSGSSSGSP KARKLPPPRP PSTLIVQHGQ QQQRLHAVTR SPRTQPSLLN ITRSSDSLPP RKADVGVQTD PVVILEEQQR HSGRDMSSSN
NT Sequence
atgaatccgt cggcgaagcg gcacgcatac gagctgctgc tgccgcagca gcaggagccc acaaacccgc tgtctgctca tcagagttgc agaggaagca caggtgccag tggtgagaac accacccttc cacagctctc gtcaaactcg gtacctgcga cgatccacat caccgagcct tctgctaact ctactctcat gttccactca gcagcagcgt cgactttggt gcccatcctc cacgggcgga cgggtgctgc agcagctgcg agagatgtca atgacggcaa tgctggaatg caccctcgtc ccagaaggag ggtaagccaa agctaccccg gggcgctcac ctcagcggtg ccgtccatgg cgctggcggt ggttcccttc acgggcagtg acggcgtcgg catcagctca ccacaacgtg gtgattcgag agtgggcggt gctggccagg atggaaacaa cacgcatggt agcggtgata gcgttggcta ctactctgga ccctccccgt atgccaagct ggccccgttg tcgctaagca gggagcgagt gtgcccgcac tgccttcggt cgtatgatgt ctttgacgac agcgaaggcg tcttcgacca ggcgacgttc tttcacgtgg atgcaacgac ggcgccatcg tcgctgcggc cctcaagtca tcggaaactg ctcccgccgc cgtcgcagga gggggatgag cagcagcagc agcaaccatc ggcgtcttcg cgcttgtaca ctgacaacgc aggggatggc ggaaatgcca ctgggcagct gttgtataac gggtgcagca cggcctctgg attcgatgag ggcagcagta atggcaccaa ccgcagcggc aactacattg cggccttgcg tccaccagct gctgcgttca cctcacacta cttccgtgcg ctacccccac cctcgttcat cacagcggtg ggagcccaca acgcatcgac tctgcttgcc atcgaggact accgcccatc actgctgcac gatagcccga gtggtgagga ggaagcgagg cagccgtgca cagtggctcc cggacctgct agtggcgagg agggtagaga ctcgagtggg acgatggtgt gcaaagcagt tgccgtgccg cactactctt atgtgagccc cacgacctgc acagggatgc gctcgcaggt gcgactgctg cagcagcatg acgtcaagaa gacggcgaca gaggtagagc acgaaaatga ggaatggcta tcgtgcgaaa gcgactcgct cgtctactcc cccatcgctg cggagccgtc gccgaacaat ggctactacc gtcattactt caaggagctc cgccaactgg gccgaggtac ttacggaggg gtctacctct gccgccacat catgtgcggc gtcaatcttg gtgagtttgc ccttaagaag attccagttg gcgacaaagc cacctacctg cagtcggtat tgcgtgaggt gcgcattctc gaggaggttc ggcgacaccc caacgtggtc gagtacaagc acagctgggt ggaggaagcg cagctcgccg actttggtcc acccgtgcgc tgtctcttca tcctgatgga gtacgcgtcc gctggctcgc tcgacactta tctggagcgc tacggcaacc atctctccac gcttgccgtg tggtacttct tcctcagctc cgtggcaggc acggcgcacc ttcacgagaa acacatctta caccgcgact taaagcctca gaacctgttg ctggccgcga tggagggccg tcctccacgc gtgctggtca gcgactttgg cacggcagcc ttgctggggg acatctcgta tgaccggtcc ggcggtacag ggacgctcga gtacatggcg ccggagctct tggagacggc cgcctcgcca cacggcatca acgagcggta cgtgaaccat cacacgatgg cctccgacgt gtggtcgctg gggatggtgc tgcactacct cgccttcgac gggacgctgc cggagcgacg cgaggacggc agtgtcaacc tcgacgaggc acaccgctcg gccaacgaca ggccgcctga actgctcaaa ctgctcgagg ctatgctgca gctcgatccc gcaaggcggc cgcgctgcag cgacatcctc ggctccccga tggtccagtc catcatgcgc atattcaaca aagatgacca cagccagtgg gacctctcca tccaggagca gcagcgcctg gccactgcgc cgtctgcgat cctcccgttg gagcgtccat cccgcccacc ggtgtcggta ccacctccca ggagcgcacc actcaagctt ccagagccct cgagtatcga tgtcagaaac tcacccccca gcgctcgcag ccgcaactct ataaccgcca tctccaactc ctcaccgggg cgcggcatgc gggctgaccg cacgaatgcg aaggacaaga gcaacgcact tcacagcagc cgtgttgagc tcctctcagc gtcggcactg ggggacacat cgacggtggc agcgcgtggc ggaagcagcg gcagcagcag cggtagcccc aaggccagga agttgcctcc accgcgccct ccgtctactt tgatcgttca gcatggccag cagcagcagc gcctgcatgc ggtgaccaga tcaccgcgga cacaaccttc cctcttgaac ataactcggt cttcagactc gctaccgcca cgcaaggctg acgtcggcgt gcaaaccgat ccggtagtga tcctggagga gcagcaacga cacagtggga gggacatgag tagtagtaac taa
Details for LebrA.20227.a.B2.GE42790
HARVESTED ON: 1/9/2019
SEQUENCED ON: 1/15/2019
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHGY YRHYFKELRQ LGRGTYGGVY LCRHIMCGVN LGEFALKKIP VGDKATYLQS VLREVRILEE VRRHPNVVEY KHSWVEEAQL ADFGPPVRCL FILMEYASAG SLDTYLERYG NHLSTLAVWY FFLSSVAGTA HLHEKHILHR DLKPQNLLLA AMEGRPPRVL VSDFGTAALL GDISYDRSGG TGTLEYMAPE LLETAASPHG INERYVNHHT MASDVWSLGM VLHYLAFDGT LPERREDGSV NLDEAHRSAN DRPPELLKLL EAMLQLDPAR RPRCSDILGS PMVQSIMRIF NKDDHSQWDL SI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggctact accgtcatta cttcaaggag ctccgccaac tgggccgagg tacttacgga ggggtctacc tctgccgcca catcatgtgc ggcgtcaatc ttggtgagtt tgcccttaag aagattccag ttggcgacaa agccacctac ctgcagtcgg tattgcgtga ggtgcgcatt ctcgaggagg ttcggcgaca ccccaacgtg gtcgagtaca agcacagctg ggtggaggaa gcgcagctcg ccgactttgg tccacccgtg cgctgtctct tcatcctgat ggagtacgcg tccgctggct cgctcgacac ttatctggag cgctacggca accatctctc cacgcttgcc gtgtggtact tcttcctcag ctccgtggca ggcacggcgc accttcacga gaaacacatc ttacaccgcg acttaaagcc tcagaacctg ttgctggccg cgatggaggg ccgtcctcca cgcgtgctgg tcagcgactt tggcacggca gccttgctgg gggacatctc gtatgaccgg tccggcggta cagggacgct cgagtacatg gcgccggagc tcttggagac ggccgcctcg ccacacggca tcaacgagcg gtacgtgaac catcacacga tggcctccga cgtgtggtcg ctggggatgg tgctgcacta cctcgccttc gacgggacgc tgccggagcg acgcgaggac ggcagtgtca acctcgacga ggcacaccgc tcggccaacg acaggccgcc tgaactgctc aaactgctcg aggctatgct gcagctcgat cccgcaaggc ggccgcgctg cagcgacatc ctcggctccc cgatggtcca gtccatcatg cgcatattca acaaagatga ccacagccag tgggacctct ccatctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat