SSGCID: Target Page

LebrA.20203.a

tyrosyl-tRNA synthetase, putative

CENTER ID:	LebrA.20203.a
ORGANISM:	Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LebrA.20203.a.B1.GE42523	full length		1	685

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LebrA.20203.a.B1.PW38514	full length		1	685

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LbrM.14.1560
UniProt:	A4H7V7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNADERYELL RSVGEECIQD AELRSLIERK PAIRCYDGFE PSGRMHIAQG IFKAVNVNKC TTAGCEFVFW VADWFALMND KVGGELEKIR IVGRYLIEVW KAAGMDMDKV VFLWSSEEIT NHAGTYWRTV LDVGRQNTIA RIKKCCTIMG KTEGTLTAAQ VLYPLMQCTD IFFLKADICQ LGLDQRKVNM LAREYCDFIG RKLKPVILSH HMLAGLKQGQ AKMSKSDPDS AIFMEDTEED VTRKIRQAYC PRVKQSANVI TDDGAPVATD DRNPVLDYFQ CVVYARPGAV ATIDGATYAT YEDLEQAFVS DKVSEVALKS CLIDEVNALL APVRQHFASN PEAHELLETV KSYRKDGAVL PLAEAALPAA PEKPHACMWM PALVKVPLDV AEGMIQAAKD FIAAHSEGTV TLVLPDWSAV ASDEITGMEK DISAALHVNS TLLKAYGLPE AVKLVMENDV ILGNPNDFWV SVIGIARKNL LSHVEELYGG ELRNAGQVIA ALMRVATTLM LSASHVISTS LDGQINTFAR EYTKGRIECL QAQEGRVPAL HRPGAAPAVL GADDVLYLDD NDMDIRRKIK KAYSAPNEEA NPIISVAQHL LAQQGVLSIE RGEANGGSVR YDTPEGLVAD CSSGALHPAD LKATVSQLLL HRSAAARALL SGELKKSIAT LRNMEKMAKR RSTNK

NT Sequence

atgaacgcgg acgagcgcta cgagcttctc cgcagcgtcg gagaggagtg cattcaagac gctgagctac ggagcctcat agaaaggaaa ccggcgattc gttgctacga cggcttcgag ccctccggcc gcatgcatat cgcccagggc atcttcaagg cagtcaatgt taacaagtgc acgacagccg ggtgcgagtt cgtcttctgg gtggcggact ggtttgcgct gatgaacgac aaggtcgggg gcgagctgga gaagattcgc attgttggcc ggtacctgat tgaagtgtgg aaggcggcgg gcatggacat ggacaaggtc gtgtttttgt ggagcagcga ggagattacg aaccacgccg gcacgtactg gcgcacggtc ctcgacgttg gtcggcagaa cacgatcgcc cgcatcaaga agtgctgcac catcatgggc aagacagagg gtacgctgac ggccgcacag gtgctgtacc cgctgatgca gtgcactgac atattcttcc tcaaggccga tatatgccag ctgggcctcg accagcgcaa ggtgaacatg ctcgcacgcg aatactgcga cttcatcggt cgcaagctga agccagtcat tctgtcgcac cacatgctgg cgggtctgaa gcaggggcag gcaaagatga gcaagagtga cccagacagt gccatcttca tggaagacac cgaggaagat gtgacacgta agatacgcca ggcttactgc ccgcgcgtca aacaatctgc caacgtcatc acggatgacg gagcaccggt cgccacggac gaccgcaacc cggtgctgga ctacttccag tgtgttgtct acgccagacc cggcgcggtg gccactattg acggcgccac gtacgctact tacgaagacc tcgaacaggc gttcgtaagc gacaaagtca gcgaggttgc cctcaagtca tgcctgattg atgaggtgaa cgcgctgctg gcgccggtgc gccagcattt tgcctcgaac ccggaggctc acgagctgct ggagacagtc aagtcgtacc gcaaagatgg cgcggtgttg ccactggcag aggcggcgct ccctgctgcc cccgaaaagc cgcacgcgtg catgtggatg ccggcgctgg tgaaggtgcc gctagatgtt gcggagggca tgatccaggc ggccaaggac ttcattgctg cccactcaga gggcacggtg acactggtgc tgccggactg gtccgcggtg gccagtgacg aaatcactgg gatggagaag gacatctccg ctgccctgca tgtcaactct acgcttctca aggcgtatgg actaccggag gcggtgaagc tcgtgatgga gaacgacgtt attctcggca accccaacga cttctgggtg agcgtaatcg gcattgctcg caagaacctg ctgagccatg tggaggagct ctacggagga gagctgcgta acgccggcca ggttatcgcg gctctcatgc gcgttgccac gacgctgatg ctctccgcct cgcacgtcat ctcgacctcg ctggatgggc agattaacac cttcgcccgc gagtacacga aggggcgtat cgagtgtctg caggcacagg aggggagagt cccggcgctg catcgccccg gcgctgcccc ggcggtgctc ggtgccgacg acgttctcta cctggacgac aatgacatgg acatccgccg taaaatcaag aaggcgtact cggcaccaaa cgaggaggcc aacccaatca tatcggttgc ccagcacctt ctcgcgcagc agggagtcct cagtatcgag cgcggcgaag ccaacggtgg cagcgtgcgc tacgacacgc cagagggcct cgttgcggac tgcagcagcg gcgctctcca ccctgctgac ctcaaggcaa cggtgtcaca actgctcctg caccggtcag cagcggcgcg cgcgttactc agtggtgagc tcaagaagag catcgctact cttcgcaaca tggagaagat ggcaaagagg cgatcaacga acaagtga

Details for LebrA.20203.a.B1.GE42523

HARVESTED ON:	7/3/2018
SEQUENCED ON:	7/10/2018
EXPECTED MW:	76kDa
OBSERVED MW:	76kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMN ADERYELLRS VGEECIQDAE LRSLIERKPA IRCYDGFEPS GRMHIAQGIF KAVNVNKCTT AGCEFVFWVA DWFALMNDKV GGELEKIRIV GRYLIEVWKA AGMDMDKVVF LWSSEEITNH AGTYWRTVLD VGRQNTIARI KKCCTIMGKT EGTLTAAQVL YPLMQCTDIF FLKADICQLG LDQRKVNMLA REYCDFIGRK LKPVILSHHM LAGLKQGQAK MSKSDPDSAI FMEDTEEDVT RKIRQAYCPR VKQSANVITD DGAPVATDDR NPVLDYFQCV VYARPGAVAT IDGATYATYE DLEQAFVSDK VSEVALKSCL IDEVNALLAP VRQHFASNPE AHELLETVKS YRKDGAVLPL AEAALPAAPE KPHACMWMPA LVKVPLDVAE GMIQAAKDFI AAHSEGTVTL VLPDWSAVAS DEITGMEKDI SAALHVNSTL LKAYGLPEAV KLVMENDVIL GNPNDFWVSV IGIARKNLLS HVEELYGGEL RNAGQVIAAL MRVATTLMLS ASHVISTSLD GQINTFAREY TKGRIECLQA QEGRVPALHR PGAAPAVLGA DDVLYLDDND MDIRRKIKKA YSAPNEEANP IISVAQHLLA QQGVLSIERG EANGGSVRYD TPEGLVADCS SGALHPADLK ATVSQLLLHR SAAARALLSG ELKKSIATLR NMEKMAKRRS TNK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg cggacgagcg ctacgagctt ctccgcagcg tcggagagga gtgcattcaa gacgctgagc tacggagcct catagaaagg aaaccggcga ttcgttgcta cgacggcttc gagccctccg gccgcatgca tatcgcccag ggcatcttca aggcagtcaa tgttaacaag tgcacgacag ccgggtgcga gttcgtcttc tgggtggcgg actggtttgc gctgatgaac gacaaggtcg ggggcgagct ggagaagatt cgcattgttg gccggtacct gattgaagtg tggaaggcgg cgggcatgga catggacaag gtcgtgtttt tgtggagcag cgaggagatt acgaaccacg ccggcacgta ctggcgcacg gtcctcgacg ttggtcggca gaacacgatc gcccgcatca agaagtgctg caccatcatg ggcaagacag agggtacgct gacggccgca caggtgctgt acccgctgat gcagtgcact gacatattct tcctcaaggc cgatatatgc cagctgggcc tcgaccagcg caaggtgaac atgctcgcac gcgaatactg cgacttcatc ggtcgcaagc tgaagccagt cattctgtcg caccacatgc tggcgggtct gaagcagggg caggcaaaga tgagcaagag tgacccagac agtgccatct tcatggaaga caccgaggaa gatgtgacac gtaagatacg ccaggcttac tgcccgcgcg tcaaacaatc tgccaacgtc atcacggatg acggagcacc ggtcgccacg gacgaccgca acccggtgct ggactacttc cagtgtgttg tctacgccag acccggcgcg gtggccacta ttgacggcgc cacgtacgct acttacgaag acctcgaaca ggcgttcgta agcgacaaag tcagcgaggt tgccctcaag tcatgcctga ttgatgaggt gaacgcgctg ctggcgccgg tgcgccagca ttttgcctcg aacccggagg ctcacgagct gctggagaca gtcaagtcgt accgcaaaga tggcgcggtg ttgccactgg cagaggcggc gctccctgct gcccccgaaa agccgcacgc gtgcatgtgg atgccggcgc tggtgaaggt gccgctagat gttgcggagg gcatgatcca ggcggccaag gacttcattg ctgcccactc agagggcacg gtgacactgg tgctgccgga ctggtccgcg gtggccagtg acgaaatcac tgggatggag aaggacatct ccgctgccct gcatgtcaac tctacgcttc tcaaggcgta tggactaccg gaggcggtga agctcgtgat ggagaacgac gttattctcg gcaaccccaa cgacttctgg gtgagcgtaa tcggcattgc tcgcaagaac ctgctgagcc atgtggagga gctctacgga ggagagctgc gtaacgccgg ccaggttatc gcggctctca tgcgcgttgc cacgacgctg atgctctccg cctcgcacgt catctcgacc tcgctggatg ggcagattaa caccttcgcc cgcgagtaca cgaaggggcg tatcgagtgt ctgcaggcac aggaggggag agtcccggcg ctgcatcgcc ccggcgctgc cccggcggtg ctcggtgccg acgacgttct ctacctggac gacaatgaca tggacatccg ccgtaaaatc aagaaggcgt actcggcacc aaacgaggag gccaacccaa tcatatcggt tgcccagcac cttctcgcgc agcagggagt cctcagtatc gagcgcggcg aagccaacgg tggcagcgtg cgctacgaca cgccagaggg cctcgttgcg gactgcagca gcggcgctct ccaccctgct gacctcaagg caacggtgtc acaactgctc ctgcaccggt cagcagcggc gcgcgcgtta ctcagtggtg agctcaagaa gagcatcgct actcttcgca acatggagaa gatggcaaag aggcgatcaa cgaacaagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for LebrA.20203.a.B1.PW38514

PURIFICATION DATe:	7/23/2018
CONCENTRATION:	32.61mg/ml
OBSERVED MW:	75kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACG CGGACGAGCG CTACGAGCTT CTCCGCAGCG TCGGAGAGGA GTGCATTCAA GACGCTGAGC TACGGAGCCT CATAGAAAGG AAACCGGCGA TTCGTTGCTA CGACGGCTTC GAGCCCTCCG GCCGCATGCA TATCGCCCAG GGCATCTTCA AGGCAGTCAA TGTTAACAAG TGCACGACAG CCGGGTGCGA GTTCGTCTTC TGGGTGGCGG ACTGGTTTGC GCTGATGAAC GACAAGGTCG GGGGCGAGCT GGAGAAGATT CGCATTGTTG GCCGGTACCT GATTGAAGTG TGGAAGGCGG CGGGCATGGA CATGGACAAG GTCGTGTTTT TGTGGAGCAG CGAGGAGATT ACGAACCACG CCGGCACGTA CTGGCGCACG GTCCTCGACG TTGGTCGGCA GAACACGATC GCCCGCATCA AGAAGTGCTG CACCATCATG GGCAAGACAG AGGGTACGCT GACGGCCGCA CAGGTGCTGT ACCCGCTGAT GCAGTGCACT GACATATTCT TCCTCAAGGC CGATATATGC CAGCTGGGCC TCGACCAGCG CAAGGTGAAC ATGCTCGCAC GCGAATACTG CGACTTCATC GGTCGCAAGC TGAAGCCAGT CATTCTGTCG CACCACATGC TGGCGGGTCT GAAGCAGGGG CAGGCAAAGA TGAGCAAGAG TGACCCAGAC AGTGCCATCT TCATGGAAGA CACCGAGGAA GATGTGACAC GTAAGATACG CCAGGCTTAC TGCCCGCGCG TCAAACAATC TGCCAACGTC ATCACGGATG ACGGAGCACC GGTCGCCACG GACGACCGCA ACCCGGTGCT GGACTACTTC CAGTGTGTTG TCTACGCCAG ACCCGGCGCG GTGGCCACTA TTGACGGCGC CACGTACGCT ACTTACGAAG ACCTCGAACA GGCGTTCGTA AGCGACAAAG TCAGCGAGGT TGCCCTCAAG TCATGCCTGA TTGATGAGGT GAACGCGCTG CTGGCGCCGG TGCGCCAGCA TTTTGCCTCG AACCCGGAGG CTCACGAGCT GCTGGAGACA GTCAAGTCGT ACCGCAAAGA TGGCGCGGTG TTGCCACTGG CAGAGGCGGC GCTCCCTGCT GCCCCCGAAA AGCCGCACGC GTGCATGTGG ATGCCGGCGC TGGTGAAGGT GCCGCTAGAT GTTGCGGAGG GCATGATCCA GGCGGCCAAG GACTTCATTG CTGCCCACTC AGAGGGCACG GTGACACTGG TGCTGCCGGA CTGGTCCGCG GTGGCCAGTG ACGAAATCAC TGGGATGGAG AAGGACATCT CCGCTGCCCT GCATGTCAAC TCTACGCTTC TCAAGGCGTA TGGACTACCG GAGGCGGTGA AGCTCGTGAT GGAGAACGAC GTTATTCTCG GCAACCCCAA CGACTTCTGG GTGAGCGTAA TCGGCATTGC TCGCAAGAAC CTGCTGAGCC ATGTGGAGGA GCTCTACGGA GGAGAGCTGC GTAACGCCGG CCAGGTTATC GCGGCTCTCA TGCGCGTTGC CACGACGCTG ATGCTCTCCG CCTCGCACGT CATCTCGACC TCGCTGGATG GGCAGATTAA CACCTTCGCC CGCGAGTACA CGAAGGGGCG TATCGAGTGT CTGCAGGCAC AGGAGGGGAG AGTCCCGGCG CTGCATCGCC CCGGCGCTGC CCCGGCGGTG CTCGGTGCCG ACGACGTTCT CTACCTGGAC GACAATGACA TGGACATCCG CCGTAAAATC AAGAAGGCGT ACTCGGCACC AAACGAGGAG GCCAACCCAA TCATATCGGT TGCCCAGCAC CTTCTCGCGC AGCAGGGAGT CCTCAGTATC GAGCGCGGCG AAGCCAACGG TGGCAGCGTG CGCTACGACA CGCCAGAGGG CCTCGTTGCG GACTGCAGCA GCGGCGCTCT CCACCCTGCT GACCTCAAGG CAACGGTGTC ACAACTGCTC CTGCACCGGT CAGCAGCGGC GCGCGCGTTA CTCAGTGGTG AGCTCAAGAA GAGCATCGCT ACTCTTCGCA ACATGGAGAA GATGGCAAAG AGGCGATCAA CGAACAAGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT