SSGCID: Target Page

LebrA.20202.a

hypothetical protein, conserved

CENTER ID:	LebrA.20202.a
ORGANISM:	Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LebrA.20202.a.B1.GE42522	full length		1	495

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LebrA.20202.a.B1.PW38616	full length		1	495

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LbrM.15.0700
UniProt:	A4H839

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHSHTAASFR RVGELACQRD SFLRELTATV VSCEPTKAAS TAAKKAKSVG DGAAAYDVIL SDSILFPEGG GQPCDFGTLR VVPGDENKAD STCSATTAPL LSITNVRRVA DACVLTSPAS LPVGTTVHQI VDWPRRIDHM QHHTGQHLLT AVVERMDTLH LPTVSWSLTH PYCFIQVDVG AYASDPASPY HAYITKEKKI SDDMVAKIEA LCNDAIIHST PVQCDVFESL EVYQQEQDRR HQAAAASTGR GEEVFRSRGI PEDVTGPIRI ISLGAIDSCT CCGTHLHTLA ELQAMYLLHQ EVKGTMVKLY FITGERAARQ FHTMYQRERQ LMPELSGSRP SEFVSVVQRR SKNTADLEKL VKRWTLELAS AEAQRIAAAV SSRHSGTVIV PLRRDDVELE FFSEVRARLD ATGLRHVVLV SAWATERCTT GTLVNTKDVA GQVFITGGES PADMEKAVQL AKETLKGMKG GSSKLGFRGK GTLKEWDALV AQLHG

NT Sequence

atgcacagcc atacggcggc ttcgttcagg cgcgtcggtg agctggcgtg tcagagagac tcgtttctgc gagaactgac cgccactgtc gtctcgtgtg agccgaccaa ggcggcatcc actgcggcga agaaggcgaa gagtgtcggt gatggtgccg cggcgtacga cgttattctg agcgactcta ttctttttcc agagggcggc ggtcagccgt gcgacttcgg cacacttcga gttgtgcctg gcgatgaaaa taaggctgat tctacgtgca gcgccaccac cgcgcctctg ctgtccatta cgaacgtgcg ccgggtcgcg gatgcgtgcg tgctcaccag ccccgcctcc ctccctgtgg ggactacagt gcaccagata gtggactggc cgcgtcggat cgaccacatg cagcaccaca ccggccagca cctgctcact gccgtggtgg agcgcatgga cacgctgcac ctgcccactg tgagctggtc cctgacacac ccttactgct tcatccaggt ggatgttggc gcctacgcaa gcgacccagc ctcgccgtac cacgcctaca tcaccaaaga gaagaaaata tcggatgata tggtggcgaa gattgaggcg ctatgcaacg acgcgatcat acacagcacc cccgtgcagt gcgacgtatt cgagagcctc gaggtgtacc agcaggagca ggaccgccgg catcaggccg cggcggcctc gacaggccgc ggcgaagagg tctttcgcag ccgtggcatc ccagaggacg taacaggacc gattcgtatc atttcgctgg gcgccatcga cagctgcacc tgctgcggca ctcatctgca cactctcgca gagctgcagg ccatgtacct cctccaccaa gaagtgaaag ggacgatggt gaagctctat tttatcaccg gcgagcgggc agcgcggcag ttccacacca tgtatcagcg ggagcggcaa ttgatgccgg agttgagtgg cagcaggccc agcgaatttg tctccgtggt gcagcgccgc tccaagaata cggcagacct tgagaagctg gtgaagcggt ggacgctcga gctagcaagc gcagaagcgc agcgcatcgc cgccgccgtg tcatcgcggc acagcggtac cgtcatagta cccctgcgcc gcgacgacgt agagctcgag ttcttcagcg aggtccgcgc acggctcgac gcgacaggac tgcgccacgt cgttctcgtt tctgcgtggg cgacggagag gtgtacgact ggcacgctcg tcaacacgaa ggatgtggcc ggccaggtct ttatcactgg cggggagtca ccggcggaca tggagaaggc cgtccagctg gcaaaagaaa cactaaaggg gatgaaaggt ggcagctcca agctcggctt ccgtgggaag ggaaccctga aggagtggga cgcccttgta gcgcagctgc atggataa

Details for LebrA.20202.a.B1.GE42522

HARVESTED ON:	7/3/2018
SEQUENCED ON:	7/10/2018
EXPECTED MW:	55kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMH SHTAASFRRV GELACQRDSF LRELTATVVS CEPTKAASTA AKKAKSAGDG AAAYDVILSD SILFPEGGGQ PCDFGTLRVV PGDENKADST CSATAAPLLS ITNVRRVGDA CVLTSPAFLP VGTTVQQTVD WPRRIDHMQH HTGQHLLTAV VERIDTLHLP TVSWSLTHPY CFIQVDVGAY ASDPASPYHP YITKEKKISD DMVAKIEALC NDAIIHSTPV QCDVFESLEV YQQEQDRRHQ AAAASTGLGE EVFRSRGIPE DVTGPIRIIS LDAIDSCTCC GTHLHTLAEL QAMYLLHQEV KGTMVKLYFI TGERAARQFH TMYQRERQLM PELSGSRPSE FVSVVQRRSK NTADLEKLVK RWTLELASAE AQRIAAAVSS RDSGTVIVPL RRDDVELEFF SEVRARLDAT GLRRVVLVSA WATERCTTGT LVNTKDVAGQ VFITGGESPA DMEKAVQLAK ETLKGMKGGS SKLGFRGKGT LKEWD

Validated NT Sequence

atggctcacc accaccacca ccatatgcac agccatacgg cggcttcgtt caggcgcgtc ggtgagctgg cgtgtcagag agactcgttt ctgcgagaac tgaccgccac tgtcgtctcg tgtgagccga ccaaggcggc atccactgcg gcgaagaagg cgaagagtgc cggtgatggt gccgcggcgt acgacgttat tctgagtgac tctattcttt ttccagaggg cggcggtcag ccgtgcgact tcggcacact tcgagttgtg cctggcgatg aaaataaggc tgattctacg tgcagcgcca ccgccgcgcc tctgctgtcc attacgaacg tgcgccgggt cggggatgcg tgcgttctca ccagccccgc cttcctccct gtggggacta cagtgcagca gacggtggac tggccgcgtc ggatcgacca catgcagcac cacaccggcc agcacctgct cactgccgtg gtggagcgca tagacacgct gcacctgccc actgtgagct ggtccctgac acacccttac tgcttcatcc aggtggatgt tggcgcctac gcaagcgacc cagcctcgcc gtaccacccc tacattacca aagagaagaa aatatcggat gatatggtgg cgaagattga ggcgctatgc aacgacgcga tcatacacag cacccccgtg cagtgcgacg tattcgagag cctcgaggtg taccagcagg agcaggaccg ccggcatcag gccgcggcgg cctcgacagg cctcggcgaa gaggtctttc gcagtcgtgg catcccagag gacgtaacag gaccgattcg tatcatttcg ctggacgcca tcgacagctg cacctgctgc ggcactcatc tgcacactct cgcagagctg caggccatgt acctcctcca ccaagaagtg aaagggacga tggtgaagct ctatttcatc accggcgagc gggcagcgcg gcagttccac accatgtatc agcgggagcg gcaattgatg ccggagttga gtggcagcag gcccagcgaa tttgtctccg tggtgcagcg ccgctccaag aatacagcag accttgagaa gctggtgaag cggtggacgc tcgagctagc aagcgcagaa gcgcagcgca tcgccgccgc cgtgtcatcg cgggacagcg gtaccgtcat agtacccctg cgccgcgacg acgtagagct cgagttcttc agcgaggtcc gcgcacggct cgacgcgaca ggactgcgcc gcgtcgttct cgtttctgcg tgggcgacgg agaggtgtac gactggcacg ctcgtcaaca cgaaggatgt ggccggccag gtctttatca ctggcgggga gtcaccggcg gacatggaga aggccgtcca gctggcaaaa gaaacactaa aggggatgaa aggtggcagc tccaagctcg gcttccgtgg gaagggaacc ctgaaggagt gggacgc

Expected Protein Sequence

MAHHHHHHMH SHTAASFRRV GELACQRDSF LRELTATVVS CEPTKAASTA AKKAKSVGDG AAAYDVILSD SILFPEGGGQ PCDFGTLRVV PGDENKADST CSATTAPLLS ITNVRRVADA CVLTSPASLP VGTTVHQIVD WPRRIDHMQH HTGQHLLTAV VERMDTLHLP TVSWSLTHPY CFIQVDVGAY ASDPASPYHA YITKEKKISD DMVAKIEALC NDAIIHSTPV QCDVFESLEV YQQEQDRRHQ AAAASTGRGE EVFRSRGIPE DVTGPIRIIS LGAIDSCTCC GTHLHTLAEL QAMYLLHQEV KGTMVKLYFI TGERAARQFH TMYQRERQLM PELSGSRPSE FVSVVQRRSK NTADLEKLVK RWTLELASAE AQRIAAAVSS RHSGTVIVPL RRDDVELEFF SEVRARLDAT GLRHVVLVSA WATERCTTGT LVNTKDVAGQ VFITGGESPA DMEKAVQLAK ETLKGMKGGS SKLGFRGKGT LKEWDALVAQ LHG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaca gccatacggc ggcttcgttc aggcgcgtcg gtgagctggc gtgtcagaga gactcgtttc tgcgagaact gaccgccact gtcgtctcgt gtgagccgac caaggcggca tccactgcgg cgaagaaggc gaagagtgtc ggtgatggtg ccgcggcgta cgacgttatt ctgagcgact ctattctttt tccagagggc ggcggtcagc cgtgcgactt cggcacactt cgagttgtgc ctggcgatga aaataaggct gattctacgt gcagcgccac caccgcgcct ctgctgtcca ttacgaacgt gcgccgggtc gcggatgcgt gcgtgctcac cagccccgcc tccctccctg tggggactac agtgcaccag atagtggact ggccgcgtcg gatcgaccac atgcagcacc acaccggcca gcacctgctc actgccgtgg tggagcgcat ggacacgctg cacctgccca ctgtgagctg gtccctgaca cacccttact gcttcatcca ggtggatgtt ggcgcctacg caagcgaccc agcctcgccg taccacgcct acatcaccaa agagaagaaa atatcggatg atatggtggc gaagattgag gcgctatgca acgacgcgat catacacagc acccccgtgc agtgcgacgt attcgagagc ctcgaggtgt accagcagga gcaggaccgc cggcatcagg ccgcggcggc ctcgacaggc cgcggcgaag aggtctttcg cagccgtggc atcccagagg acgtaacagg accgattcgt atcatttcgc tgggcgccat cgacagctgc acctgctgcg gcactcatct gcacactctc gcagagctgc aggccatgta cctcctccac caagaagtga aagggacgat ggtgaagctc tattttatca ccggcgagcg ggcagcgcgg cagttccaca ccatgtatca gcgggagcgg caattgatgc cggagttgag tggcagcagg cccagcgaat ttgtctccgt ggtgcagcgc cgctccaaga atacggcaga ccttgagaag ctggtgaagc ggtggacgct cgagctagca agcgcagaag cgcagcgcat cgccgccgcc gtgtcatcgc ggcacagcgg taccgtcata gtacccctgc gccgcgacga cgtagagctc gagttcttca gcgaggtccg cgcacggctc gacgcgacag gactgcgcca cgtcgttctc gtttctgcgt gggcgacgga gaggtgtacg actggcacgc tcgtcaacac gaaggatgtg gccggccagg tctttatcac tggcggggag tcaccggcgg acatggagaa ggccgtccag ctggcaaaag aaacactaaa ggggatgaaa ggtggcagct ccaagctcgg cttccgtggg aagggaaccc tgaaggagtg ggacgccctt gtagcgcagc tgcatggatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for LebrA.20202.a.B1.PW38616

PURIFICATION DATe:	5/14/2019
CONCENTRATION:	11.72mg/ml
OBSERVED MW:	55kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	6
VIAL VOLUME:	55µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCACA GCCATACGGC GGCTTCGTTC AGGCGCGTCG GTGAGCTGGC GTGTCAGAGA GACTCGTTTC TGCGAGAACT GACCGCCACT GTCGTCTCGT GTGAGCCGAC CAAGGCGGCA TCCACTGCGG CGAAGAAGGC GAAGAGTGTC GGTGATGGTG CCGCGGCGTA CGACGTTATT CTGAGCGACT CTATTCTTTT TCCAGAGGGC GGCGGTCAGC CGTGCGACTT CGGCACACTT CGAGTTGTGC CTGGCGATGA AAATAAGGCT GATTCTACGT GCAGCGCCAC CACCGCGCCT CTGCTGTCCA TTACGAACGT GCGCCGGGTC GCGGATGCGT GCGTGCTCAC CAGCCCCGCC TCCCTCCCTG TGGGGACTAC AGTGCACCAG ATAGTGGACT GGCCGCGTCG GATCGACCAC ATGCAGCACC ACACCGGCCA GCACCTGCTC ACTGCCGTGG TGGAGCGCAT GGACACGCTG CACCTGCCCA CTGTGAGCTG GTCCCTGACA CACCCTTACT GCTTCATCCA GGTGGATGTT GGCGCCTACG CAAGCGACCC AGCCTCGCCG TACCACGCCT ACATCACCAA AGAGAAGAAA ATATCGGATG ATATGGTGGC GAAGATTGAG GCGCTATGCA ACGACGCGAT CATACACAGC ACCCCCGTGC AGTGCGACGT ATTCGAGAGC CTCGAGGTGT ACCAGCAGGA GCAGGACCGC CGGCATCAGG CCGCGGCGGC CTCGACAGGC CGCGGCGAAG AGGTCTTTCG CAGCCGTGGC ATCCCAGAGG ACGTAACAGG ACCGATTCGT ATCATTTCGC TGGGCGCCAT CGACAGCTGC ACCTGCTGCG GCACTCATCT GCACACTCTC GCAGAGCTGC AGGCCATGTA CCTCCTCCAC CAAGAAGTGA AAGGGACGAT GGTGAAGCTC TATTTTATCA CCGGCGAGCG GGCAGCGCGG CAGTTCCACA CCATGTATCA GCGGGAGCGG CAATTGATGC CGGAGTTGAG TGGCAGCAGG CCCAGCGAAT TTGTCTCCGT GGTGCAGCGC CGCTCCAAGA ATACGGCAGA CCTTGAGAAG CTGGTGAAGC GGTGGACGCT CGAGCTAGCA AGCGCAGAAG CGCAGCGCAT CGCCGCCGCC GTGTCATCGC GGCACAGCGG TACCGTCATA GTACCCCTGC GCCGCGACGA CGTAGAGCTC GAGTTCTTCA GCGAGGTCCG CGCACGGCTC GACGCGACAG GACTGCGCCA CGTCGTTCTC GTTTCTGCGT GGGCGACGGA GAGGTGTACG ACTGGCACGC TCGTCAACAC GAAGGATGTG GCCGGCCAGG TCTTTATCAC TGGCGGGGAG TCACCGGCGG ACATGGAGAA GGCCGTCCAG CTGGCAAAAG AAACACTAAA GGGGATGAAA GGTGGCAGCT CCAAGCTCGG CTTCCGTGGG AAGGGAACCC TGAAGGAGTG GGACGCCCTT GTAGCGCAGC TGCATGGATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT