SSGCID: Target Page

LebrA.19898.a

Uncharacterized protein (CRK12)

CENTER ID:	LebrA.19898.a
ORGANISM:	Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LebrA.19898.a.B1.GE42068	full length		1	883

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LbrM.09.0300
UniProt:	A4H5K5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQHPRRNSRG SASPSCEGHN YAKAIIVEDD ERGEEAISRG AGRGPSVGGG DGGTSCASNS PLTSSSGGSV HEAQPPPAVA TLATAPLGLV ASGIDEPLCG EQAHSIRTRC RETEAPTTPP PQLHSVVNGA TRAVDASGRA STARIMRLDP VTPSFVSSLS PHPVAHHTLR LQPSAASLQP LPHARQLQER VDEEEGVPPS KRLRPSLSKP IASAATPPLA TAPRGICTAD VGSSGSRVLR LVLSSCSATP ADTAKTSART PASVSAAGGG GGNGAATTCV NVSAHGEALK GGRREAAEEA ESPAKANEEA QCTSDTPAHA PLGVSPPLPL ASPAAATIVK AEASGIGVAP LPAAPASRPP PILTSVTTRA ITANALNVGS PVSASVASVV AQQHHIRPTT SGASPSGAIE TSGFPRLRSL TPTPYPGAQG SPCRRHRFAG VNLPGAPPPS IDFGSIQRTV MDVYEVHEKL SEGTYGEVFK GVDKRTGAAV ALKRLKILST HQGFPQTSLR EIIALRHIQS QRERLEERLR NDVHHRATVA ITDPLAEVSQ LCDVLLYDRQ QRDIVLVFAY ATASLAGLCR RQFAFTPSEM AFLMKKLLIA VRKLHEMRII HRDIKSDNVL VTWEGEVQLT DFGLCSIVAS GPSQSGAPVW RTPSVITLAY RPPEMLLGST AYDEKVDVWS LGCLLAQMYL LEPPFYRHRA QPQQRAPERS AATELDQLSR ITEVLGPLPP VRVYHPDSCQ HMRVLEQLER QGRLAESGQA TQPPNWGKLQ TIFEPSFLYQ QFHGFRGWFE AELERSRHQP RRRPTQACMD VLCAALQLDP QQRPSAAELL RMPYFTTLDD APLLGSYQRV LSVTPEREAE VRHGFMIKVR RCGDSHTQRR LHQ

NT Sequence

atgcagcatc cacgtagaaa cagccgtggc tctgcgtccc cctcctgtga gggtcacaac tatgcaaagg cgatcatagt agaggatgac gaaaggggag aggaggcgat tagcaggggc gctggtcgag gtccaagcgt cggcggcggc gatggtggca ccagctgtgc cagcaactct cccctcacct ccagctcagg cggcagcgtg cacgaagccc agccaccacc cgcggtggcc acattggcaa cagcgccact ggggctagtc gcgtcaggca tagacgaacc tctatgcggt gagcaggcac actccatccg cactaggtgc agagaaacag aggcgccaac aacaccacca ccacagctgc attcagtcgt caatggcgcc actagggctg tggatgctag tggtagggca agtacagcgc gaatcatgcg gctagacccc gtcacgccct ccttcgtctc ttctttgtct ccccatcctg tggctcatca cacgctgcgc ctccagccct ccgccgcctc acttcagccc cttccccacg cacggcagct gcaggagcgg gttgatgaag aggaaggggt gcccccctca aagcggctgc gtccatctct gagcaaaccc attgcctctg cagctacacc tcctttggca acggcacctc gtggtatatg taccgcagat gtcggcagca gcggcagccg tgtcttgcgg ctcgtattgt caagttgcag tgccactcca gccgacactg caaagacgtc agctcgtacg ccggcgtccg tctccgctgc gggcggtggt ggtggtaacg gtgcggccac cacatgcgtc aacgtctccg cccatggcga ggcgctcaaa gggggcagaa gagaagcagc cgaagaagca gagagcccgg ccaaggccaa cgaggaggca cagtgtacgt cagacacgcc ggcgcatgcc cctttgggtg tctcaccacc tcttccacta gcctccccag ccgccgccac catcgtcaag gctgaggcga gtggcatcgg tgtcgcacct ctcccagcgg ccccagcgtc gcgcccaccg ccgatcctca caagcgtcac cacacgcgcc atcactgcca acgcgctaaa cgtggggtcg cccgtgtcgg cctccgtggc gtcggtcgtc gcgcaacagc atcatattcg accgaccaca tctggtgcaa gcccttccgg agcgatagag acaagcggct tcccgcgcct acgctcgctt acaccaacac cataccccgg cgcgcaaggg tccccatgcc ggcgtcaccg ctttgcgggg gtaaacctcc ccggcgctcc gccgccatct attgactttg gctccatcca gcgcactgtg atggacgtct atgaggtgca cgagaagctc agcgaaggca cgtatggcga ggtcttcaag ggcgtcgaca agcgaactgg cgctgcggtg gcactgaagc gtctcaagat actgagcacc caccagggct ttccacagac ctcgttgcgg gagataattg cccttcgaca catccagagc cagcgcgagc ggctcgagga acgactgcgc aacgacgtgc atcaccgcgc cacggtggcc atcaccgacc ctctcgcgga ggtctcgcaa ctctgtgatg ttcttctcta cgatcggcag cagcgcgaca ttgtgctggt gttcgcgtac gccacggcga gtctggccgg cctgtgtcgc cgccagttcg cctttactcc gtcggagatg gccttcctca tgaagaaact cctcatcgcg gtacgcaagc tgcatgagat gcgcattatt caccgcgaca ttaagtccga taatgtgttg gtaacgtggg aaggcgaggt gcagttgacc gactttgggc tgtgctccat cgtcgcatcc gggccgtcgc agtctggcgc gcctgtgtgg cggacaccaa gcgtgattac cctggcctac cgtccaccag agatgctgct gggcagcacc gcgtatgatg agaaggtgga tgtgtggtcg cttgggtgcc tgctcgccca gatgtacctt ctcgagcccc ccttttacag gcaccgcgcg cagccacagc agcgtgcgcc tgagcgatcg gcggctacgg aactagacca gctctcccgc attacagagg tgctaggacc gctgccaccg gtgcgcgtct atcaccctga ctcgtgtcag cacatgcgcg ttctggaaca actggagagg caggggcgtt tggctgagtc tgggcaagcc acgcagccgc ccaactgggg caagctgcag accatcttcg agccaagttt cctttatcag caattccacg gttttcgggg ttggtttgag gcagagctgg aacgctcgcg gcaccagcca cgccgtcggc ccacgcaagc ttgcatggac gtactctgcg ccgccttaca gctcgacccg cagcagcgcc cctccgcggc agagctgctg cgcatgccat actttaccac cttagatgac gccccgttgc tgggtagcta tcaacgggtc ctctccgtca ccccggaacg ggaggctgag gtgcgccacg gcttcatgat aaaggtgcga cgatgtggag acagccacac gcaacgccgc ctgcatcagt aa

Details for LebrA.19898.a.B1.GE42068

HARVESTED ON:	data unavailable
SEQUENCED ON:	12/7/2017
EXPECTED MW:	96kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMQ HPRRNSRGSA SPSCEGHNYA KAIIVEDDER GEEAISRGAG RGPSVGGGDG GTSCASNSPL TSSSGGSVHE AQPPPAVATL ATAPLGLVAS GIDEPLCGEQ AHSIRTRCRE TEAPTTPPPQ LHSVVNGATR AVDASGRAST ARIMRLDPVT PSFVSSLSPH PVAHHTLRLQ PSAASLQPLP HARQLQERVD EEEGVPPSKR LRPSLSKPIA SAATPPLATA PRGICTADVG SSGSRVLRLV LSSCSATPAD TAKTSARTPA SVSAAGGGGG NGAATTCVNV SAHGEALKGG RREAAEEAES PAKANEEAQC TSDTPAHAPL GVSPPLPLAS PAAATIVKAE ASGIGVAPLP AAPASRPPPI LTSVTTRAIT ANALNVGSPV SASVASVVAQ QHHIRPTTSG ASPSGAIETS GFPRLRSLTP TPYPGAQGSP CRRHRFAGVN LPGAPPPSID FGSIQRTVMD VYEVHEKLSE GTYGEVFKGV DKRTGAAVAL KRLKILSTHQ GFPQTSLREI IALRHIQSQR ERLEERLRND VHHRATVAIT DPLAEVSQLC DVLLYDRQQR DIVLVFAYAT ASLAGLCRRQ FAFTPSEMAF LMKKLLIAVR KLHEMRIIHR DIKSDNVLVT WEGEVQLTDF GLCSIVASGP SQSGAPVWRT PSVITLAYRP PEMLLGSTAY DEKVDVWSLG CLLAQMYLLE PPFYRHRAQP QQRAPERSAA TELDQLSRIT EVLGPLPPVR VYHPDSCQHM RVLEQLERQG RLAESGQATQ PPNWGKLQTI FEPSFLYQQF HGFRGWFEAE LERSRHQPRR RPTQACMDVL CAALQLDPQQ RPSAAELLRM PYFTTLDDAP LLGSYQRVLS VTPEREAEVR HGFMIKVRRC GDSHTQRRLH Q

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagc atccacgtag aaacagccgt ggctctgcgt ccccctcctg tgagggtcac aactatgcaa aggcgatcat agtagaggat gacgaaaggg gagaggaggc gattagcagg ggcgctggtc gaggtccaag cgtcggcggc ggcgatggtg gcaccagctg tgccagcaac tctcccctca cctccagctc aggcggcagc gtgcacgaag cccagccacc acccgcggtg gccacattgg caacagcgcc actggggcta gtcgcgtcag gcatagacga acctctatgc ggtgagcagg cacactccat ccgcactagg tgcagagaaa cagaggcgcc aacaacacca ccaccacagc tgcattcagt cgtcaatggc gccactaggg ctgtggatgc tagtggtagg gcaagtacag cgcgaatcat gcggctagac cccgtcacgc cctccttcgt ctcttctttg tctccccatc ctgtggctca tcacacgctg cgcctccagc cctccgccgc ctcacttcag ccccttcccc acgcacggca gctgcaggag cgggttgatg aagaggaagg ggtgcccccc tcaaagcggc tgcgtccatc tctgagcaaa cccattgcct ctgcagctac acctcctttg gcaacggcac ctcgtggtat atgtaccgca gatgtcggca gcagcggcag ccgtgtcttg cggctcgtat tgtcaagttg cagtgccact ccagccgaca ctgcaaagac gtcagctcgt acgccggcgt ccgtctccgc tgcgggcggt ggtggtggta acggtgcggc caccacatgc gtcaacgtct ccgcccatgg cgaggcgctc aaagggggca gaagagaagc agccgaagaa gcagagagcc cggccaaggc caacgaggag gcacagtgta cgtcagacac gccggcgcat gcccctttgg gtgtctcacc acctcttcca ctagcctccc cagccgccgc caccatcgtc aaggctgagg cgagtggcat cggtgtcgca cctctcccag cggccccagc gtcgcgccca ccgccgatcc tcacaagcgt caccacacgc gccatcactg ccaacgcgct aaacgtgggg tcgcccgtgt cggcctccgt ggcgtcggtc gtcgcgcaac agcatcatat tcgaccgacc acatctggtg caagcccttc cggagcgata gagacaagcg gcttcccgcg cctacgctcg cttacaccaa caccataccc cggcgcgcaa gggtccccat gccggcgtca ccgctttgcg ggggtaaacc tccccggcgc tccgccgcca tctattgact ttggctccat ccagcgcact gtgatggacg tctatgaggt gcacgagaag ctcagcgaag gcacgtatgg cgaggtcttc aagggcgtcg acaagcgaac tggcgctgcg gtggcactga agcgtctcaa gatactgagc acccaccagg gctttccaca gacctcgttg cgggagataa ttgcccttcg acacatccag agccagcgcg agcggctcga ggaacgactg cgcaacgacg tgcatcaccg cgccacggtg gccatcaccg accctctcgc ggaggtctcg caactctgtg atgttcttct ctacgatcgg cagcagcgcg acattgtgct ggtgttcgcg tacgccacgg cgagtctggc cggcctgtgt cgccgccagt tcgcctttac tccgtcggag atggccttcc tcatgaagaa actcctcatc gcggtacgca agctgcatga gatgcgcatt attcaccgcg acattaagtc cgataatgtg ttggtaacgt gggaaggcga ggtgcagttg accgactttg ggctgtgctc catcgtcgca tccgggccgt cgcagtctgg cgcgcctgtg tggcggacac caagcgtgat taccctggcc taccgtccac cagagatgct gctgggcagc accgcgtatg atgagaaggt ggatgtgtgg tcgcttgggt gcctgctcgc ccagatgtac cttctcgagc ccccctttta caggcaccgc gcgcagccac agcagcgtgc gcctgagcga tcggcggcta cggaactaga ccagctctcc cgcattacag aggtgctagg accgctgcca ccggtgcgcg tctatcaccc tgactcgtgt cagcacatgc gcgttctgga acaactggag aggcaggggc gtttggctga gtctgggcaa gccacgcagc cgcccaactg gggcaagctg cagaccatct tcgagccaag tttcctttat cagcaattcc acggttttcg gggttggttt gaggcagagc tggaacgctc gcggcaccag ccacgccgtc ggcccacgca agcttgcatg gacgtactct gcgccgcctt acagctcgac ccgcagcagc gcccctccgc ggcagagctg ctgcgcatgc catactttac caccttagat gacgccccgt tgctgggtag ctatcaacgg gtcctctccg tcaccccgga acgggaggct gaggtgcgcc acggcttcat gataaaggtg cgacgatgtg gagacagcca cacgcaacgc cgcctgcatc agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat