LebrA.19676.a

Putative tRNA binding domain containing protein, putative

CENTER ID: LebrA.19676.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.19676.a.B1.GE42670 full length 1 424
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.22.0420
UniProt: A4HC93

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQFVIHSSSI LAPALQHVLR SILSVPAPWV ETSAKGPHRV TVDGQTFHGL QSFLQALRRT AVTPEQKAFL GGDEEQILLV NQWVCAAAVL DVDATRADAD TSTSVAKFLY SDVERILAAM SNAASQYLTG SERATMADLL MYAAAFNHFA HAEVLPTTMK WAAYAQEDAY LAPIRSAAVK VPVNNKAGKA SGVAAPATRA EVSYVKPSEE EILRRRAEKE KAKAEKAAAA AAGGASTEKA STSPSTGAAS SNKAKKVELD STSLYVRVGQ LTNLRRHPDA DRLYVEDMVL GDETRTIVSG LVEHYAMEEL EGTQCLVVCN MKPKPLIGIT SQGMVLCAKN GTEVKLIRPP AGAKPGDRIL FGTTYDTAVA SAGAPQPMSG NKISEVLSHL HTNADGVLCW KDKPARHPSG VEVCVPDMAN CPVS
NT Sequence
atgcaatttg tgattcactc ctcctccatc ctcgcgccgg cactgcagca tgtgctcagg agcatcttga gcgtgccggc cccgtgggtt gagacatctg ccaaaggccc tcaccgcgtt acagttgatg gacagacctt ccacgggctc cagtcgtttc tgcaggcgct acgccgcacc gcggttacgc cggagcagaa ggcgtttctc ggcggagacg aggagcagat attgctggta aaccagtggg tttgcgcggc ggcggtgctg gatgttgatg ctacgcgcgc tgatgccgac acttccacct cggtcgccaa gtttctctac agtgacgtgg agcgcatcct cgcggccatg tcgaatgcgg cgtcgcagta cctcaccgga agcgagcgcg ccaccatggc cgacttgctc atgtacgccg ccgccttcaa ccactttgcc cacgcggagg tgctgccgac caccatgaaa tgggccgcat acgcacaaga ggacgcctac ctggccccca ttcgctcggc ggctgttaag gtgccggtga acaacaaggc tggcaaggct agcggcgtgg ccgcgcctgc tactagggcg gaagtctcat atgtgaagcc gtctgaggag gagattctgc gccgccgtgc agagaaggag aaagcgaagg cggagaaggc tgctgccgcc gcagcaggag gagcaagcac ggagaaagcc tcgaccagtc ccagtactgg agctgcaagc agcaacaaag ccaagaaagt ggagctcgac tctaccagcc tgtacgtgcg tgtggggcag ctgacaaacc tgcgtcgaca cccggacgca gaccgcctct acgtcgaaga tatggtgctc ggcgatgaga cgcgcaccat tgtaagtgga ctcgtggagc actacgcgat ggaggagcta gagggaacac agtgcctcgt cgtgtgcaat atgaagccga agccgcttat cggtatcacg tcgcagggga tggtgctgtg cgccaagaac ggcacagagg tcaagttgat tcgtcctccg gcgggcgcca aaccaggaga ccgcatcctt tttggtacca cgtacgatac ggcggtcgcc tccgctggcg ctccacagcc gatgtcgggt aacaagataa gcgaggtgct ctcccacctg cataccaacg ccgacggcgt tctttgctgg aaggataagc ctgcaagaca cccgagcggt gtggaagtct gcgttcccga catggcaaac tgtcccgtga gctga
Details for LebrA.19676.a.B1.GE42670
HARVESTED ON: data unavailable
SEQUENCED ON: 10/17/2018
EXPECTED MW: 46kDa
OBSERVED MW: 75kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMQ FVIHSSSILA PALQHALRSI LSVPAPWVET SAKGPHRVTV DGQTFHGLQS FLQALRRTAV TPEQKAFLGG DEEQILLVNQ WVCAAAVLDV DATCADADTS TSVAKFLYSD VERILAAMSN AASQYLTGSE RATMADLLMY AAAFNHSAHA EVLPTTMKWA AYAQEDAYLA PIRSAAVKVP VNNKAGKASG VAAPATRAEV SYVKPSEEEI LRRRAEKEKA KAEKAAAAAA GGASTEKAST SPSTGAASSN KAKKVELDST SLYVRVGQLT NLRRHPDADR LYVEDMVLGD ETRTIVSGLV EHYAMEELEG TQCLVVCNMK PKLLIGITSQ GMVLCAKNGT EVKLIRPPAG AKPGDRILFG TTYDTAVAIA GAPEPMPGNK ISEVLSHLHT NADGVLCWKD KPARHPSGVE VCVP
Validated NT Sequence
cgggaacgca gacttccaca ccgcttgggt gtcttgcagg cttatccttc cagcaaagaa cgccgtcggc gttggtatgc aggtgggaga gcacctcgct tattttgtta cccggcatcg gctctggagc gccagcgatg gcgaccgccg tatcgtacgt ggtaccaaaa aggatgcggt cgcctggttt ggcgcccgcc ggaggacgaa tcaacttgac ctctgtgccg ttcttggcgc acagcaccat cccctgcgac gtgataccga taagcagctt cggcttcata ttgcacacga cgaggcactg tgttccctcc agctcctcca tcgcgtagtg ctccacgagt ccacttacaa tggtgcgcgt ctcatcgccg agcaccatat cttcgacgta gaggcggtct gcgtccgggt gtcgacgcag gtttgtcagc tgccccacac gcacgtacag gctggtagag tcgagctcca ctttcttggc tttgttgctg cttgcagctc cagtactggg actggtcgag gctttctccg tgcttgctcc tcctgctgcg gcggcagcag ccttctccgc cttcgctttc tccttctccg cacggcggcg cagaatctcc tcttcagacg gcttcacata tgagacttcc gccctagtag caggcgcggc cacgccgcta gccttgccag ccttgttgtt caccggcacc ttaacagccg ccgagcgaat gggggccagg taggcgtcct cttgtgcgta tgcagcccat ttcatggtgg tcggcagcac ctccgcgtgg gcagagtggt tgaaggcggc ggcgtacatg agcaagtcgg ccatggtggc gcgctcgctt ccggtgaggt actgcgacgc cgcgttcgac atggccgcga ggatgcgctc cacgtcactg tagagaaact tggcgaccga ggtggaagtg tcggcatcag cgcacgtagc atcaacatcc agcaccgccg ccgcgcaaac ccactggttt accagcaata tctgctcctc gtctccgccg agaaacgcct tctgctccgg cgtaaccgcg gtgcggcgta gcgcctgcag aaacgactgg agcccgtgga aggtctgtcc atcgactgta acgcggtgag ggcctttggc agatgtctca acccacgggg ccggcacgct caagatgctc ctgagcgcat gctgcagtgc cggcgcgagg atggaggagg agtgaatcac aaattgcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMQ FVIHSSSILA PALQHVLRSI LSVPAPWVET SAKGPHRVTV DGQTFHGLQS FLQALRRTAV TPEQKAFLGG DEEQILLVNQ WVCAAAVLDV DATRADADTS TSVAKFLYSD VERILAAMSN AASQYLTGSE RATMADLLMY AAAFNHFAHA EVLPTTMKWA AYAQEDAYLA PIRSAAVKVP VNNKAGKASG VAAPATRAEV SYVKPSEEEI LRRRAEKEKA KAEKAAAAAA GGASTEKAST SPSTGAASSN KAKKVELDST SLYVRVGQLT NLRRHPDADR LYVEDMVLGD ETRTIVSGLV EHYAMEELEG TQCLVVCNMK PKPLIGITSQ GMVLCAKNGT EVKLIRPPAG AKPGDRILFG TTYDTAVASA GAPQPMSGNK ISEVLSHLHT NADGVLCWKD KPARHPSGVE VCVPDMANCP VS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaat ttgtgattca ctcctcctcc atcctcgcgc cggcactgca gcatgtgctc aggagcatct tgagcgtgcc ggccccgtgg gttgagacat ctgccaaagg ccctcaccgc gttacagttg atggacagac cttccacggg ctccagtcgt ttctgcaggc gctacgccgc accgcggtta cgccggagca gaaggcgttt ctcggcggag acgaggagca gatattgctg gtaaaccagt gggtttgcgc ggcggcggtg ctggatgttg atgctacgcg cgctgatgcc gacacttcca cctcggtcgc caagtttctc tacagtgacg tggagcgcat cctcgcggcc atgtcgaatg cggcgtcgca gtacctcacc ggaagcgagc gcgccaccat ggccgacttg ctcatgtacg ccgccgcctt caaccacttt gcccacgcgg aggtgctgcc gaccaccatg aaatgggccg catacgcaca agaggacgcc tacctggccc ccattcgctc ggcggctgtt aaggtgccgg tgaacaacaa ggctggcaag gctagcggcg tggccgcgcc tgctactagg gcggaagtct catatgtgaa gccgtctgag gaggagattc tgcgccgccg tgcagagaag gagaaagcga aggcggagaa ggctgctgcc gccgcagcag gaggagcaag cacggagaaa gcctcgacca gtcccagtac tggagctgca agcagcaaca aagccaagaa agtggagctc gactctacca gcctgtacgt gcgtgtgggg cagctgacaa acctgcgtcg acacccggac gcagaccgcc tctacgtcga agatatggtg ctcggcgatg agacgcgcac cattgtaagt ggactcgtgg agcactacgc gatggaggag ctagagggaa cacagtgcct cgtcgtgtgc aatatgaagc cgaagccgct tatcggtatc acgtcgcagg ggatggtgct gtgcgccaag aacggcacag aggtcaagtt gattcgtcct ccggcgggcg ccaaaccagg agaccgcatc ctttttggta ccacgtacga tacggcggtc gcctccgctg gcgctccaca gccgatgtcg ggtaacaaga taagcgaggt gctctcccac ctgcatacca acgccgacgg cgttctttgc tggaaggata agcctgcaag acacccgagc ggtgtggaag tctgcgttcc cgacatggca aactgtcccg tgagctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat