LebrA.19528.a

J-binding protein 2 (JBP2)

CENTER ID:	LebrA.19528.a
ORGANISM:	Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LebrA.19528.a.B1.GE41455	full length		1	1098
LebrA.19528.a.B2.GE41456	N-term conserved domain		6	167

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_79439

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LbrM.14.0040
UniProt:	A4H3H5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPSGLMRANT STELESILDI VQSSGEIAVV FTSPSIGDLE TITSETQRRQ LRIAGIPRGG YTILPAIPLY DDELLQMCER YTAASEHEKV EIRNSLYMRE YPLFAYSMRN QRALFHPADY VSRILQFCFH YVQVPDEDVL SLQDRSPFLH ISPVKEICVH LRLIVRGTPA APDESESPVP EQLHFHAESD AEKLAAERAR ALSIAASSGG ASETEPLSLF TGVAPSALFQ KGAVEEVDLD TEETIEDLTG EETVDAVHSF HSEYLTLSGF ELVTKASIFY DHEGEGQRVV AVYIPGGVPK ETCRAAAAVL ELAVTKKNLR AATNGGLPPD TGIVGYYDYL TNPTQHKCRE TEFSRRNWGL FSQSESLLKH LDKLYSQLAP THHHLQRVAI PSQYQLCGTV FSTITVNRNF RTAVHTDKGD FRSGLGVLSV INGEFEGCHL AIKSLKKAFQ LKVGDVLLFD TSLEHGNTEV VHPENHWQRT SIVCYLRTGL MSSVCEMERR KHLNRLILQQ LLNTEIRHTT VNINEADSSL PPLFVPTRLA SQLAPVQLAA LGFIVERTNK QSGCVVAMTM GLGKTLVALT LCFSHLHLAP QADILILTPK PIISHWVDEK NKWGMYGLHF PHFVASDGLN SLEFEQQLLE YERQKNNEKP KAGHVFVINS EYLAGFLRRF RRFTPFLIIV DEGHRVAAKG NKLTESLDRL RCNLRVVLSG TPLQNDASEL YRLVGWVNKG VSKVLPPKRF QELANSINQF VEGDDGAFYN AVMAQEYIQD WMRGFVFREM ENDLPPLHDY LLVCGSSNVQ REYEEKLGLT ETAMTALRAT EHRPHHLSTH PACYLAFISN CYQSMVSGWT VRAQSNTSRL RTTQLEEIDA MRLEQYAQMI ENEQLDAFIN VSGKMRVLVD IVLRVQARKE KLIIFSLYVG SQDLIHRTLT ALRVCTFTVR GRDSQDRRRR AMHEFSENKD LTVLVLSTKI AAYGLEFTAA NHVVLFDSWW NPQADAQAIA RAYRRNQRKP VTVYRLISAT ENKFVLRSQT RKIALFKCIF HKRTTRQALP SELEDCSANE TDNERRDFWA KLKATHLVGD TRALLNVYRY QESVRESQ

NT Sequence

atgccaagtg gtctgatgcg ggcgaacacc agcacggagc tggagtcgat actggatatc gtgcaatcgt ccggcgaaat cgctgttgtc ttcacttcgc cgagcattgg cgatttggag acgattacaa gcgaaacgca gcgtcgtcag ctgcgcatcg ctgggatccc tcgcggtggg tacaccatcc tccccgctat tccgctatac gatgacgagc tgcttcagat gtgtgagcgg tacaccgccg cgagtgaaca cgagaaggtg gaaattcgga acagcctgta tatgcgagaa tacccactat tcgcgtacag catgcgcaac cagagagcac tgtttcaccc tgctgactac gtaagccgca ttctccagtt ctgcttccat tatgttcagg tgcccgacga agacgtttta tcgctgcagg acaggagtcc ctttcttcac atctcccccg tcaaagaaat ttgcgtacat ctccgcctca tcgtccgtgg cactccagca gcaccagatg agtccgagtc accggtgcca gagcagctgc actttcatgc agagtcagac gctgagaagc ttgcagccga gcgtgcgcgc gctttgtcca tcgcagcaag cagtggcggc gcctcagaga cggagccgct ctctctcttc accggcgttg cgccgtcggc gctgtttcag aaaggggcag tggaagaagt ggacctggac actgaggaaa ccatcgagga tctcactggc gaggagacag tagatgcggt gcacagcttt cattcagagt acttgacatt gagcgggttt gagttagtga caaaagcatc catcttctac gaccacgaag gagagggaca gcgcgtcgtt gcggtgtaca ttccaggagg tgtgccaaag gagacgtgcc gcgcagcagc agctgtccta gagcttgcag tgacaaaaaa gaatttgcgg gcagccacga acggtggtct gccaccggac accggaatcg tgggctacta tgactacctc acaaatccta cccagcacaa atgcagagag acagaattca gccgcaggaa ctggggcctc ttctcacaga gtgagtcgct actgaagcat ctcgacaagc tgtacagcca gttggcccca acgcaccatc atctgcagcg agtagcgatc ccgtcgcagt atcagctgtg cggcacagtc ttcagcacca tcactgtcaa ccgaaacttc cgtaccgccg tgcacactga caagggtgac tttcggagcg gcttgggggt tctctcagta atcaacggcg agttcgaggg ctgtcatctc gccatcaaaa gtctcaaaaa ggcgtttcag ctcaaagttg gcgatgttct tcttttcgac acgtctctgg agcacggcaa cacggaggtg gtgcacccag aaaatcactg gcagcggacc agcattgtgt gctacctgcg cactggacta atgtcctctg tgtgcgagat ggagcgacga aagcatttaa atcggctcat tcttcagcag ctgctcaaca cggagattcg ccacaccacc gtcaatatca atgaagccga tagcagcctg ccgccgctct ttgtgccgac ccgcctggcg agccagttgg ctccagtgca gcttgccgct ctcggcttca ttgtggagcg gacgaacaag cagagcggat gtgtggtggc gatgacgatg gggctgggca aaaccctcgt ggcattaacg ttgtgcttct cacacctgca tcttgcaccg caggcggaca tcctcatcct cactccaaag ccgattatca gtcattgggt ggacgagaag aacaagtggg gcatgtacgg actccacttt cctcacttcg tcgcctctga cgggctcaac tccttagagt tcgagcagca gctcctcgag tacgagcggc agaagaacaa cgagaaacca aaggcaggcc acgtcttcgt catcaacagc gagtacctgg cagggtttct gcgacgcttc aggcgattta cgcccttcct cattatagtg gacgagggtc accgagttgc agcgaaggga aataagctga cggagtcact cgatcgccta cggtgcaacc tccgtgttgt gttgtccggc acgcctttgc aaaacgatgc cagcgaactc taccggctag tggggtgggt gaacaaaggc gtcagcaagg tactgccgcc gaaacgcttc caggagcttg ctaatagcat caaccagttc gtcgagggcg atgatggggc cttctacaat gcagtgatgg cgcaagagta catccaagac tggatgcgtg ggttcgtgtt tcgtgaaatg gaaaacgacc tgccaccgct gcacgattac ctgttggtat gcggctcctc caacgtgcag cgggagtatg aagagaagtt gggcctcaca gagacggcca tgacagcctt gagggcaacg gagcaccgac cacatcacct ctccacacac cccgcgtgct acctggcctt catctccaac tgctaccaat cgatggtaag cgggtggaca gtgcgcgcgc agtctaatac gtcacgactg cgaacaaccc agctggaaga gatcgatgcc atgcgtcttg agcagtacgc ccagatgatt gaaaacgagc aactcgacgc cttcatcaac gtgagtggca agatgcgggt gctcgtcgat attgtgcttc gcgtgcaggc tcgcaaggag aagctcatca ttttctccct ctatgtggga tcgcaagatc tcattcatcg aactctcaca gcgctgcggg tctgcacatt tacggttcgt ggccgagact cacaagaccg ccgccgccgc gccatgcacg agttcagcga aaacaaagac ctcactgtgc tggtattgtc aacaaagatc gctgcctacg gtctcgaatt caccgctgct aatcatgtcg ttctgttcga ctcgtggtgg aacccgcagg cggacgcaca ggcaatcgcc cgcgcctaca ggcgaaatca acggaagccg gtgaccgtgt atcgccttat ctctgcgacc gagaacaagt tcgtgttacg ctcacagaca cgaaaaattg ccttgttcaa gtgcatcttc cacaagcgca ccacccgaca ggcgctacca agtgaactag aggactgctc agctaacgag accgataacg agcggcggga cttctgggca aagctaaagg cgacccacct cgttggtgat actcgagctt tgctgaacgt gtatcggtac caagagagcg tacgcgaaag tcaatga

Details for LebrA.19528.a.B1.GE41455

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	125kDa
OBSERVED MW:	125kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMP SGLMRANTST ELESILDIVQ SSGEIAVVFT SPSIGDLETI TSETQRRQLR IAGIPRGGYT ILPAIPLYDD ELLQMCERYT AASEHEKVEI RNSLYMREYP LFAYSMRNQR ALFHPADYVS RILQFCFHYV QVPDEDVLSL QDRSPFLHIS PVKEICVHLR LIVRGTPAAP DESESPVPEQ LHFHAESDAE KLAAERARAL SIAASSGGAS ETEPLSLFTG VAPSALFQKG AVEEVDLDTE ETIEDLTGEE TVDAVHSFHS EYLTLSGFEL VTKASIFYDH EGEGQRVVAV YIPGGVPKET CRAAAAVLEL AVTKKNLRAA TNGGLPPDTG IVGYYDYLTN PTQHKCRETE FSRRNWGLFS QSESLLKHLD KLYSQLAPTH HHLQRVAIPS QYQLCGTVFS TITVNRNFRT AVHTDKGDFR SGLGVLSVIN GEFEGCHLAI KSLKKAFQLK VGDVLLFDTS LEHGNTEVVH PENHWQRTSI VCYLRTGLMS SVCEMERRKH LNRLILQQLL NTEIRHTTVN INEADSSLPP LFVPTRLASQ LAPVQLAALG FIVERTNKQS GCVVAMTMGL GKTLVALTLC FSHLHLAPQA DILILTPKPI ISHWVDEKNK WGMYGLHFPH FVASDGLNSL EFEQQLLEYE RQKNNEKPKA GHVFVINSEY LAGFLRRFRR FTPFLIIVDE GHRVAAKGNK LTESLDRLRC NLRVVLSGTP LQNDASELYR LVGWVNKGVS KVLPPKRFQE LANSINQFVE GDDGAFYNAV MAQEYIQDWM RGFVFREMEN DLPPLHDYLL VCGSSNVQRE YEEKLGLTET AMTALRATEH RPHHLSTHPA CYLAFISNCY QSMVSGWTVR AQSNTSRLRT TQLEEIDAMR LEQYAQMIEN EQLDAFINVS GKMRVLVDIV LRVQARKEKL IIFSLYVGSQ DLIHRTLTAL RVCTFTVRGR DSQDRRRRAM HEFSENKDLT VLVLSTKIAA YGLEFTAANH VVLFDSWWNP QADAQAIARA YRRNQRKPVT VYRLISATEN KFVLRSQTRK IALFKCIFHK RTTRQALPSE LEDCSANETD NERRDFWAKL KATHLVGDTR ALLNVYRYQE SVRESQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccaa gtggtctgat gcgggcgaac accagcacgg agctggagtc gatactggat atcgtgcaat cgtccggcga aatcgctgtt gtcttcactt cgccgagcat tggcgatttg gagacgatta caagcgaaac gcagcgtcgt cagctgcgca tcgctgggat ccctcgcggt gggtacacca tcctccccgc tattccgcta tacgatgacg agctgcttca gatgtgtgag cggtacaccg ccgcgagtga acacgagaag gtggaaattc ggaacagcct gtatatgcga gaatacccac tattcgcgta cagcatgcgc aaccagagag cactgtttca ccctgctgac tacgtaagcc gcattctcca gttctgcttc cattatgttc aggtgcccga cgaagacgtt ttatcgctgc aggacaggag tccctttctt cacatctccc ccgtcaaaga aatttgcgta catctccgcc tcatcgtccg tggcactcca gcagcaccag atgagtccga gtcaccggtg ccagagcagc tgcactttca tgcagagtca gacgctgaga agcttgcagc cgagcgtgcg cgcgctttgt ccatcgcagc aagcagtggc ggcgcctcag agacggagcc gctctctctc ttcaccggcg ttgcgccgtc ggcgctgttt cagaaagggg cagtggaaga agtggacctg gacactgagg aaaccatcga ggatctcact ggcgaggaga cagtagatgc ggtgcacagc tttcattcag agtacttgac attgagcggg tttgagttag tgacaaaagc atccatcttc tacgaccacg aaggagaggg acagcgcgtc gttgcggtgt acattccagg aggtgtgcca aaggagacgt gccgcgcagc agcagctgtc ctagagcttg cagtgacaaa aaagaatttg cgggcagcca cgaacggtgg tctgccaccg gacaccggaa tcgtgggcta ctatgactac ctcacaaatc ctacccagca caaatgcaga gagacagaat tcagccgcag gaactggggc ctcttctcac agagtgagtc gctactgaag catctcgaca agctgtacag ccagttggcc ccaacgcacc atcatctgca gcgagtagcg atcccgtcgc agtatcagct gtgcggcaca gtcttcagca ccatcactgt caaccgaaac ttccgtaccg ccgtgcacac tgacaagggt gactttcgga gcggcttggg ggttctctca gtaatcaacg gcgagttcga gggctgtcat ctcgccatca aaagtctcaa aaaggcgttt cagctcaaag ttggcgatgt tcttcttttc gacacgtctc tggagcacgg caacacggag gtggtgcacc cagaaaatca ctggcagcgg accagcattg tgtgctacct gcgcactgga ctaatgtcct ctgtgtgcga gatggagcga cgaaagcatt taaatcggct cattcttcag cagctgctca acacggagat tcgccacacc accgtcaata tcaatgaagc cgatagcagc ctgccgccgc tctttgtgcc gacccgcctg gcgagccagt tggctccagt gcagcttgcc gctctcggct tcattgtgga gcggacgaac aagcagagcg gatgtgtggt ggcgatgacg atggggctgg gcaaaaccct cgtggcatta acgttgtgct tctcacacct gcatcttgca ccgcaggcgg acatcctcat cctcactcca aagccgatta tcagtcattg ggtggacgag aagaacaagt ggggcatgta cggactccac tttcctcact tcgtcgcctc tgacgggctc aactccttag agttcgagca gcagctcctc gagtacgagc ggcagaagaa caacgagaaa ccaaaggcag gccacgtctt cgtcatcaac agcgagtacc tggcagggtt tctgcgacgc ttcaggcgat ttacgccctt cctcattata gtggacgagg gtcaccgagt tgcagcgaag ggaaataagc tgacggagtc actcgatcgc ctacggtgca acctccgtgt tgtgttgtcc ggcacgcctt tgcaaaacga tgccagcgaa ctctaccggc tagtggggtg ggtgaacaaa ggcgtcagca aggtactgcc gccgaaacgc ttccaggagc ttgctaatag catcaaccag ttcgtcgagg gcgatgatgg ggccttctac aatgcagtga tggcgcaaga gtacatccaa gactggatgc gtgggttcgt gtttcgtgaa atggaaaacg acctgccacc gctgcacgat tacctgttgg tatgcggctc ctccaacgtg cagcgggagt atgaagagaa gttgggcctc acagagacgg ccatgacagc cttgagggca acggagcacc gaccacatca cctctccaca caccccgcgt gctacctggc cttcatctcc aactgctacc aatcgatggt aagcgggtgg acagtgcgcg cgcagtctaa tacgtcacga ctgcgaacaa cccagctgga agagatcgat gccatgcgtc ttgagcagta cgcccagatg attgaaaacg agcaactcga cgccttcatc aacgtgagtg gcaagatgcg ggtgctcgtc gatattgtgc ttcgcgtgca ggctcgcaag gagaagctca tcattttctc cctctatgtg ggatcgcaag atctcattca tcgaactctc acagcgctgc gggtctgcac atttacggtt cgtggccgag actcacaaga ccgccgccgc cgcgccatgc acgagttcag cgaaaacaaa gacctcactg tgctggtatt gtcaacaaag atcgctgcct acggtctcga attcaccgct gctaatcatg tcgttctgtt cgactcgtgg tggaacccgc aggcggacgc acaggcaatc gcccgcgcct acaggcgaaa tcaacggaag ccggtgaccg tgtatcgcct tatctctgcg accgagaaca agttcgtgtt acgctcacag acacgaaaaa ttgccttgtt caagtgcatc ttccacaagc gcaccacccg acaggcgcta ccaagtgaac tagaggactg ctcagctaac gagaccgata acgagcggcg ggacttctgg gcaaagctaa aggcgaccca cctcgttggt gatactcgag ctttgctgaa cgtgtatcgg taccaagaga gcgtacgcga aagtcaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for LebrA.19528.a.B2.GE41456

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	20kDa
OBSERVED MW:	20kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMR ANTSTELESI LDIVQSSGEI AVVFTSPSIG DLETITSETQ RRQLRIAGIP RGGYTILPAI PLYDDELLQM CERYTAASEH EKVEIRNSLY MREYPLFAYS MRNQRALFHP ADYVSRILQF CFHYVQVPDE DVLSLQDRSP FLHISPVKEI CVHLRLIVRG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcggg cgaacaccag cacggagctg gagtcgatac tggatatcgt gcaatcgtcc ggcgaaatcg ctgttgtctt cacttcgccg agcattggcg atttggagac gattacaagc gaaacgcagc gtcgtcagct gcgcatcgct gggatccctc gcggtgggta caccatcctc cccgctattc cgctatacga tgacgagctg cttcagatgt gtgagcggta caccgccgcg agtgaacacg agaaggtgga aattcggaac agcctgtata tgcgagaata cccactattc gcgtacagca tgcgcaacca gagagcactg tttcaccctg ctgactacgt aagccgcatt ctccagttct gcttccatta tgttcaggtg cccgacgaag acgttttatc gctgcaggac aggagtccct ttcttcacat ctcccccgtc aaagaaattt gcgtacatct ccgcctcatc gtccgtggct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat