LebrA.19527.a

Glucosyl transferase (HmdUGT)

CENTER ID: LebrA.19527.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.19527.a.B3.GE41454 C-term domain 802 950
LebrA.19527.a.B2.GE41453 nucleoside diphospho-sugar transferase-like domain 22 736
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.19527.a.B3.PS38308 C-term domain 802 950

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.35.2590
UniProt: A4HPA4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFSLNIKTET SPFSAAAAPA PEADASEAPL ATIDVDEGDS FESTPSTNSS SGQGGRHRCQ HVPRLPQTPP SPETPIKFDH GAYPHCPIFI PSKGRSDLDR STMAVLVRDL VPFVLVVERE EVEGYSALLD RLVGLYFGFS DMTFASKVDD GTEGATKTEA EDVAPFHTAP ADLWEFSCAC CDGSPGVVGS PSSSTTPSLS ADALRALALR LSAAFHHGYV FEPPTNVAAG GQDSIDESKN SSPPVSTSPT APSAAGRGAW RPSEAFVLRN VNEVRALFMI EVLPESNRGV SYVRNYILQV LVPRLMVACG VDRSGNLEEL CLYGPGEHST LTPAALRMAT LEPSVYSALE TKSGTESYLD VRRASTITSP VTASTGSTAG TVDGGLASSV VGQSDPAGQT LAPALRSSTP SSTSAGTRGT PRILHGFFGF YWVLDDDIYG FYQSHGSSTK NERISARAMM REVEGRLRQL RRNADAASPP PPPVPPAGFQ ISPTPSSSSS ATASPYFLSH QQQQQPFVFT CKEDALQNNF ITGGKGRLPT LHNYNLYYTT ACFSLEYNRF ALDTAPDMLS VNSYNNIACL FNYALLHNPP QMRFFPSGVP HVRRPEEALV GYLDHSMLWY RFAVREDYDF TLQLIARGLY TLRFRSIAFD VPQMMKVRGG MTDYYRNCHE EIRQQNNRFV LQWSSVAQHW VKGKEGNRRD DIRVRWDLLS PARAKYPGAF LYLSTPLPQL QPQRLSDDGS SGNGSVASSS DSATTATNVE GSEAQARKRL RPPSPTPPTG ESSPAPSGRR LDVAAAAASA SVVSSTAARD WKGGYVVERW RDISHEEAWQ HAGLVALSND DIHIGRTVAV IPPFFDQKPS VVRATVIERA VEPTDSHASS GRVGNASTVE RSDVKPPPPP RVVWTVVAQD VRGMPFLHVD TCFEVPAKGI EGAIASIDQF FAKMTDEFGA
NT Sequence
atgttctctc tcaacatcaa aacagagacc tcgccgttct ccgcagctgc cgcaccagca cccgaagcgg atgccagcga ggcgccattg gcgaccattg acgtggacga aggcgactcc tttgaaagca cgccctcgac gaacagcagc agtggtcagg gcggccgcca tcgttgccag catgtccccc ggctgccaca gacaccacca agtcctgaga caccgatcaa gtttgaccat ggtgcgtacc cgcactgccc catcttcatc ccctccaaag gtcgctctga cctggaccgc agcaccatgg cggtgctcgt ccgtgacctc gtcccgtttg tgctcgtcgt cgagcgggag gaagtcgagg ggtacagcgc gctgctggac cgcctcgttg gcctttactt tggcttctca gatatgacat ttgcttcgaa agtggacgac ggcacggaag gggcaaccaa gacggaggca gaggatgtcg cgccgttcca caccgccccg gcagacctct gggagttctc gtgcgcctgc tgcgacggca gccctggcgt cgttgggtca ccgtcgtcgt ccactacgcc cagcctctcg gcagatgcct tgcgcgcgtt ggcgttgcga ctttcagctg cgtttcacca tggctacgtg ttcgaaccac caacgaatgt ggcagccggt ggccaagaca gcattgacga gtcaaaaaat agctcaccac ctgtgtcgac tagtccaaca gcgccatcag cagccggtag aggtgcatgg cgcccctctg aggcctttgt tctgcgcaac gtgaacgagg tacgcgcgct cttcatgatc gaagtgctgc cggagtccaa ccgtggggtc tcctacgtgc gcaactacat cctccaggtt ctcgtgccaa ggctaatggt ggcctgcggt gtagatcggt cggggaatct ggaggaactg tgcctgtatg gaccgggaga gcacagcacg ctgacaccgg cggcgctgcg gatggccaca ctggaaccga gcgtctacag tgccctcgag accaagtcag ggactgagtc ttatctggac gttcggcggg cgagcacaat cacgagtccg gtaacggcaa gtactggaag caccgcaggc accgtagatg gcggtctggc gtcgtcggtg gtagggcaaa gcgacccagc cggacaaacc ctcgcgcctg cgttgcgctc gtcaacgccg tcctccactt cggccgggac tcgcggcacc cctcgcatcc tgcacggctt cttcggcttc tactgggttc tcgacgatga catctacggc ttttaccagt cgcacggctc cagcaccaaa aacgagcgca tcagtgcacg ggcaatgatg agagaggtgg agggacggct gcggcagctg cggcgtaacg ccgatgcggc ctcgccacca ccacctccag taccgcccgc aggctttcag atatcgccaa cccccagcag cagcagcagc gcaacagcgt ccccgtattt cttgtcacac caacagcaac agcaaccctt tgtgttcacg tgcaaggagg atgccctgca gaacaacttc attactggcg gaaaaggacg gctaccgacg ctgcacaact acaaccttta ctacaccacc gcctgttttt cgctcgagta caaccgcttc gccctcgata cggcgccgga catgctgtcg gtgaactcct acaacaacat tgcgtgtctc ttcaactatg cgttgctgca caacccgccg cagatgcgct tcttcccctc tggtgtaccg cacgtgcggc gccccgaaga agcgctggtc ggctacctcg accacagcat gctctggtac cgcttcgccg tacgcgagga ttacgacttc actcttcagc tcatcgcacg tggactctac acactgcgct tccgaagcat tgccttcgat gtgccgcaga tgatgaaagt tcgtggcggt atgacggact actaccgcaa ctgccacgaa gagatacggc agcagaacaa tcgctttgtg ctgcagtggt cctccgtggc acagcactgg gtgaaaggga aggagggcaa ccgtcgagac gacatacgtg tgcgatggga cctactgagt ccagcccgcg ccaagtaccc cggcgccttc ttgtacctga gcaccccact gccacagctg cagccgcaac gcttgagtga cgacggcagc agcggcaacg gcagtgtagc cagcagcagt gacagtgcca ctactgcaac caatgtggaa ggctcggaag cgcaggcgcg caagcgtctg cggcccccgt ccccgactcc ccccacaggt gaatcgtcac ctgcaccttc aggccgtcgc ctcgatgtcg cggccgccgc agcctctgcg tccgtcgtgt cgtccaccgc tgcccgtgac tggaaaggcg gttatgtagt cgagcggtgg cgcgatatct cacatgagga ggcatggcaa cacgccggcc tcgtcgccct ctccaacgac gacatccaca ttggccggac tgtcgccgtc ataccgcctt tcttcgatca gaaaccatct gtcgtgcgcg cgacggtaat tgagcgggct gtcgagccaa cagattctca tgccagcagc ggtcgcgttg gaaacgccag cacggtggag agaagcgatg tcaagccgcc cccgccgccg cgcgtggtct ggacggtggt ggcacaggac gttcgcggca tgcccttctt gcacgtcgac acgtgctttg aggtacctgc gaagggcatt gagggggcca tcgcgagtat tgatcagttc ttcgcaaaga tgacagacga atttggcgcc taa
Details for LebrA.19527.a.B3.GE41454
HARVESTED ON: 4/21/2017
SEQUENCED ON: 5/3/2017
EXPECTED MW: 17kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHVV SSTAARDWKG GYVVERWRDI SHEEAWQHAG LVALSNDDIH IGRTVAVIPP FFDQKPSVVR ATVIERAVEP TDSHASSGRV GNASTVERSD VKPPPPPRVV WTVVAQDVRG MPFLHVDTCF EVPAKGIEGA IASIDQFFAK MTDEFGA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtcgtgt cgtccaccgc tgcccgtgac tggaaaggcg gttatgtagt cgagcggtgg cgcgatatct cacatgagga ggcatggcaa cacgccggcc tcgtcgccct ctccaacgac gacatccaca ttggccggac tgtcgccgtc ataccgcctt tcttcgatca gaaaccatct gtcgtgcgcg cgacggtaat tgagcgggct gtcgagccaa cagattctca tgccagcagc ggtcgcgttg gaaacgccag cacggtggag agaagcgatg tcaagccgcc cccgccgccg cgcgtggtct ggacggtggt ggcacaggac gttcgcggca tgcccttctt gcacgtcgac acgtgctttg aggtacctgc gaagggcatt gagggggcca tcgcgagtat tgatcagttc ttcgcaaaga tgacagacga atttggcgcc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for LebrA.19527.a.B2.GE41453
HARVESTED ON: 4/21/2017
SEQUENCED ON: 5/3/2017
EXPECTED MW: 79kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHEA DASEAPLATI DVDEGDSFES TPSTNSSSGQ GGRHRCQHVP RLPQTPPSPE TPIKFDHGAY PHCPIFIPSK GRSDLDRSTM AVLVRDLVPF VLVVEREEVE GYSALLDRLV GLYFGFSDMT FASKVDDGTE GATKTEAEDV APFHTAPADL WEFSCACCDG SPGVVGSPSS STTPSLSADA LRALALRLSA AFHHGYVFEP PTNVAAGGQD SIDESKNSSP PVSTSPTAPS AAGRGAWRPS EAFVLRNVNE VRALFMIEVL PESNRGVSYV RNYILQVLVP RLMVACGVDR SGNLEELCLY GPGEHSTLTP AALRMATLEP SVYSALETKS GTESYLDVRR ASTITSPVTA STGSTAGTVD GGLASSVVGQ SDPAGQTLAP ALRSSTPSST SAGTRGTPRI LHGFFGFYWV LDDDIYGFYQ SHGSSTKNER ISARAMMREV EGRLRQLRRN ADAASPPPPP VPPAGFQISP TPSSSSSATA SPYFLSHQQQ QQPFVFTCKE DALQNNFITG GKGRLPTLHN YNLYYTTACF SLEYNRFALD TAPDMLSVNS YNNIACLFNY ALLHNPPQMR FFPSGVPHVR RPEEALVGYL DHSMLWYRFA VREDYDFTLQ LIARGLYTLR FRSIAFDVPQ MMKVRGGMTD YYRNCHEEIR QQNNRFVLQW SSVAQHWVKG KEGNRRDDIR VRWDLLSPAR AKYPGAFLYL STPLPQLQPQ RLS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaagcgg atgccagcga ggcgccattg gcgaccattg acgtggacga aggcgactcc tttgaaagca cgccctcgac gaacagcagc agtggtcagg gcggccgcca tcgttgccag catgtccccc ggctgccaca gacaccacca agtcctgaga caccgatcaa gtttgaccat ggtgcgtacc cgcactgccc catcttcatc ccctccaaag gtcgctctga cctggaccgc agcaccatgg cggtgctcgt ccgtgacctc gtcccgtttg tgctcgtcgt cgagcgggag gaagtcgagg ggtacagcgc gctgctggac cgcctcgttg gcctttactt tggcttctca gatatgacat ttgcttcgaa agtggacgac ggcacggaag gggcaaccaa gacggaggca gaggatgtcg cgccgttcca caccgccccg gcagacctct gggagttctc gtgcgcctgc tgcgacggca gccctggcgt cgttgggtca ccgtcgtcgt ccactacgcc cagcctctcg gcagatgcct tgcgcgcgtt ggcgttgcga ctttcagctg cgtttcacca tggctacgtg ttcgaaccac caacgaatgt ggcagccggt ggccaagaca gcattgacga gtcaaaaaat agctcaccac ctgtgtcgac tagtccaaca gcgccatcag cagccggtag aggtgcatgg cgcccctctg aggcctttgt tctgcgcaac gtgaacgagg tacgcgcgct cttcatgatc gaagtgctgc cggagtccaa ccgtggggtc tcctacgtgc gcaactacat cctccaggtt ctcgtgccaa ggctaatggt ggcctgcggt gtagatcggt cggggaatct ggaggaactg tgcctgtatg gaccgggaga gcacagcacg ctgacaccgg cggcgctgcg gatggccaca ctggaaccga gcgtctacag tgccctcgag accaagtcag ggactgagtc ttatctggac gttcggcggg cgagcacaat cacgagtccg gtaacggcaa gtactggaag caccgcaggc accgtagatg gcggtctggc gtcgtcggtg gtagggcaaa gcgacccagc cggacaaacc ctcgcgcctg cgttgcgctc gtcaacgccg tcctccactt cggccgggac tcgcggcacc cctcgcatcc tgcacggctt cttcggcttc tactgggttc tcgacgatga catctacggc ttttaccagt cgcacggctc cagcaccaaa aacgagcgca tcagtgcacg ggcaatgatg agagaggtgg agggacggct gcggcagctg cggcgtaacg ccgatgcggc ctcgccacca ccacctccag taccgcccgc aggctttcag atatcgccaa cccccagcag cagcagcagc gcaacagcgt ccccgtattt cttgtcacac caacagcaac agcaaccctt tgtgttcacg tgcaaggagg atgccctgca gaacaacttc attactggcg gaaaaggacg gctaccgacg ctgcacaact acaaccttta ctacaccacc gcctgttttt cgctcgagta caaccgcttc gccctcgata cggcgccgga catgctgtcg gtgaactcct acaacaacat tgcgtgtctc ttcaactatg cgttgctgca caacccgccg cagatgcgct tcttcccctc tggtgtaccg cacgtgcggc gccccgaaga agcgctggtc ggctacctcg accacagcat gctctggtac cgcttcgccg tacgcgagga ttacgacttc actcttcagc tcatcgcacg tggactctac acactgcgct tccgaagcat tgccttcgat gtgccgcaga tgatgaaagt tcgtggcggt atgacggact actaccgcaa ctgccacgaa gagatacggc agcagaacaa tcgctttgtg ctgcagtggt cctccgtggc acagcactgg gtgaaaggga aggagggcaa ccgtcgagac gacatacgtg tgcgatggga cctactgagt ccagcccgcg ccaagtaccc cggcgccttc ttgtacctga gcaccccact gccacagctg cagccgcaac gcttgagttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for LebrA.19527.a.B3.PS38308
PURIFICATION DATe: 6/28/2017
CONCENTRATION: 21.6mg/ml
OBSERVED MW: 18kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer: 20mM HEPES, 500mM Nacl, 5% Glycerol, 1mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHVV SSTAARDWKG GYVVERWRDI SHEEAWQHAG LVALSNDDIH IGRTVAVIPP FFDQKPSVVR ATVIERAVEP TDSHASSGRV GNASTVERSD VKPPPPPRVV WTVVAQDVRG MPFLHVDTCF EVPAKGIEGA IASIDQFFAK MTDEFGA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTCGTGT CGTCCACCGC TGCCCGTGAC TGGAAAGGCG GTTATGTAGT CGAGCGGTGG CGCGATATCT CACATGAGGA GGCATGGCAA CACGCCGGCC TCGTCGCCCT CTCCAACGAC GACATCCACA TTGGCCGGAC TGTCGCCGTC ATACCGCCTT TCTTCGATCA GAAACCATCT GTCGTGCGCG CGACGGTAAT TGAGCGGGCT GTCGAGCCAA CAGATTCTCA TGCCAGCAGC GGTCGCGTTG GAAACGCCAG CACGGTGGAG AGAAGCGATG TCAAGCCGCC CCCGCCGCCG CGCGTGGTCT GGACGGTGGT GGCACAGGAC GTTCGCGGCA TGCCCTTCTT GCACGTCGAC ACGTGCTTTG AGGTACCTGC GAAGGGCATT GAGGGGGCCA TCGCGAGTAT TGATCAGTTC TTCGCAAAGA TGACAGACGA ATTTGGCGCC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT