LebrA.19526.a

WDR82 homologue (glucosyl transferase interacting protein 1) (GTIP1)

CENTER ID: LebrA.19526.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.19526.a.B2.GE41452 WD-40 propeller domain 24 380
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.32.4060
UniProt: A4HL19

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSFTTASPPV PSSGGTAEST AVGGVPIEVA SMFYTGINEP RPTSMNFSFD GLHFVSAHTD DAVRLIDVGN MTNTDVIICD AFGVHNVRYT HSSSVVCVAP RFHLDGHLHL LNTETSQFFG EMAYLNDAES EILPVANTPV YSTLAQCPAT DVIGAVVAAK GRLALFHPLI SGCIAATPER SIVGSRVCVQ FSLDGHRIAI GDDHRITLLD RRNLFQAPQV VLENKRVFTT SASLTRCKGL EFSADASRLL LTSSSGEVVV YDWQRDETVV NYFHGDAKRH FLGSADAIGA QYVFPYQIAS PILQLTSSMT GGRHLLVYAG CHSNSVYPTR HGRLLYELQS KDSDVPVAVA VNPRLQLAAT AARSITWWAF HSSVVEAYAR PTQAGTIAAD
NT Sequence
atgagcttca ctaccgcgag tcctcccgtt ccctccagcg gaggaactgc tgagtcgacc gccgtgggcg gagtaccaat agaggtggcg tccatgtttt acactggcat caacgagccg aggcccacct cgatgaattt ctcctttgac ggccttcact tcgtgtcggc gcatacggac gacgccgtcc gtctcatcga tgtgggcaac atgacgaaca cagacgtgat catctgcgac gcgtttggcg tgcacaacgt ccgctacacc cactccagca gcgtcgtgtg tgtcgcgccg cggtttcact tggacggcca cctgcacctt ctcaacacgg agacgtcgca gttcttcggc gagatggcgt acctcaacga cgccgagtcg gagattttgc cagtggcgaa cacgccagtg tactccactc ttgctcagtg cccggcaaca gacgtgatag gcgcggtggt ggcagccaag ggtcggctag cccttttcca ccctctcatc tctggctgca ttgctgcgac accggagcga tccatcgtag gcagtagagt ctgcgtgcag tttagcctgg acggtcatcg catcgccatc ggcgacgacc accgcatcac ccttctcgat cgacgcaacc tattccaggc accgcaggtc gtgctggaaa acaagcgcgt cttcaccacg tcagcgtcgc taacgagatg caaagggctc gagtttagcg ccgatgcaag tcgcttgctg ctcacctcgt caagtggcga ggttgtggtg tacgactggc agcgcgacga gacggttgtg aactacttcc acggcgacgc gaagcgacac tttctcggct ccgccgacgc cattggcgcg cagtacgtct ttccgtatca gattgcctcg cctattctac agctcacctc gtccatgact ggaggccggc atctgctcgt gtatgccggt tgccactcta attcggtgta cccgacccga cacgggcgac ttctgtatga gctgcagtcc aaggacagcg atgtgccggt tgctgtcgcc gtgaacccac ggctacagct agcggctact gcagcacgga gcatcacatg gtgggctttt cacagctctg tagtcgaggc ttacgcgaga ccgacgcagg cgggcacgat tgcggcggac tga
Details for LebrA.19526.a.B2.GE41452
HARVESTED ON: 4/21/2017
SEQUENCED ON: 5/3/2017
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMG VPIEVASMFY TGINEPRPTS MNFSFDGLHF VSAHTDDAVR LIDVGNMTNT DVIICDAFGV HNVRYTHSSS VVCVAPRFHL DGHLHLLNTE TSQFFGEMAY LNDAESEILP VANTPVYSTL AQCPATDVIG AVVAAKGRLA LFHPLISGCI AATPERSIVG SRVCVQFSLD GHRIAIGDDH RITLLDRRNL FQAPHVVLEN KRVFTSSASL TRCKGLEFSA DASRLLLTSS SGEVVVYDWQ RDETVVNYFH GDAKRHFLGS ADAIGAQYVF PYQTASPILQ LTSSMTGGRH LLVYAGCHSN SVYPTRHGRL LYELQSKDSD VPVAVAVNPR LQLAATAARS ITWWAFH
Validated NT Sequence
atggctcacc accaccacca ccatatggga gtaccaatag aggtggcgtc catgttttac actggcatca acgagccgag gcccacctcg atgaatttct cctttgacgg ccttcacttc gtgtcggcgc atacggacga cgccgtccgt ctcatcgatg tgggcaacat gacgaacaca gacgtgatca tctgcgacgc gtttggcgtg cacaacgtcc gctacaccca ctccagcagc gtcgtgtgtg tcgcgccgcg gtttcacttg gacggccacc tacaccttct caacacggag acgtcgcagt tcttcggcga gatggcgtac ctcaacgacg ccgagtcgga gattttgcca gtggcgaaca cgccagtgta ctccactctt gctcagtgcc cggcaacaga cgtgataggc gcggtggtgg cagccaaggg tcggctagcc cttttccacc ctctcatctc tggctgcatt gctgcgacac cggagcgatc tatcgtaggc agtagagtct gcgtgcagtt tagcctggac ggtcatcgca tcgccatcgg cgacgaccac cgcatcaccc ttctcgatcg acgcaaccta ttccaggcac cgcatgtcgt gctggaaaac aagcgcgtct tcacctcgtc agcgtcgcta acgagatgca aagggctcga gtttagcgcc gatgcgagtc gcttgctgct cacctcgtcg agtggcgagg ttgtggtgta cgactggcag cgcgacgaga cggttgtgaa ctacttccac ggcgacgcga agcgacactt tctcggctct gccgacgcca ttggcgcgca gtacgtcttt ccgtatcaga ccgcctcgcc cattctacag ctcacctcgt ccatgactgg aggccggcat ctgctcgtgt atgccggttg ccactctaat tcggtgtacc cgacccgaca cgggcgactt ctgtatgagc tgcagtccaa ggacagcgat gtaccggttg ctgtcgccgt gaacccacgg ctacagctag cggctactgc agcacggagc atcacatggt gggcttttca cagc
Expected Protein Sequence
MAHHHHHHGV PIEVASMFYT GINEPRPTSM NFSFDGLHFV SAHTDDAVRL IDVGNMTNTD VIICDAFGVH NVRYTHSSSV VCVAPRFHLD GHLHLLNTET SQFFGEMAYL NDAESEILPV ANTPVYSTLA QCPATDVIGA VVAAKGRLAL FHPLISGCIA ATPERSIVGS RVCVQFSLDG HRIAIGDDHR ITLLDRRNLF QAPQVVLENK RVFTTSASLT RCKGLEFSAD ASRLLLTSSS GEVVVYDWQR DETVVNYFHG DAKRHFLGSA DAIGAQYVFP YQIASPILQL TSSMTGGRHL LVYAGCHSNS VYPTRHGRLL YELQSKDSDV PVAVAVNPRL QLAATAARSI TWWAFHSSVV EAYAR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggagtac caatagaggt ggcgtccatg ttttacactg gcatcaacga gccgaggccc acctcgatga atttctcctt tgacggcctt cacttcgtgt cggcgcatac ggacgacgcc gtccgtctca tcgatgtggg caacatgacg aacacagacg tgatcatctg cgacgcgttt ggcgtgcaca acgtccgcta cacccactcc agcagcgtcg tgtgtgtcgc gccgcggttt cacttggacg gccacctgca ccttctcaac acggagacgt cgcagttctt cggcgagatg gcgtacctca acgacgccga gtcggagatt ttgccagtgg cgaacacgcc agtgtactcc actcttgctc agtgcccggc aacagacgtg ataggcgcgg tggtggcagc caagggtcgg ctagcccttt tccaccctct catctctggc tgcattgctg cgacaccgga gcgatccatc gtaggcagta gagtctgcgt gcagtttagc ctggacggtc atcgcatcgc catcggcgac gaccaccgca tcacccttct cgatcgacgc aacctattcc aggcaccgca ggtcgtgctg gaaaacaagc gcgtcttcac cacgtcagcg tcgctaacga gatgcaaagg gctcgagttt agcgccgatg caagtcgctt gctgctcacc tcgtcaagtg gcgaggttgt ggtgtacgac tggcagcgcg acgagacggt tgtgaactac ttccacggcg acgcgaagcg acactttctc ggctccgccg acgccattgg cgcgcagtac gtctttccgt atcagattgc ctcgcctatt ctacagctca cctcgtccat gactggaggc cggcatctgc tcgtgtatgc cggttgccac tctaattcgg tgtacccgac ccgacacggg cgacttctgt atgagctgca gtccaaggac agcgatgtgc cggttgctgt cgccgtgaac ccacggctac agctagcggc tactgcagca cggagcatca catggtgggc ttttcacagc tctgtagtcg aggcttacgc gagatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat