SSGCID: Target Page

LebrA.19512.a

KREX2

CENTER ID:	LebrA.19512.a
ORGANISM:	Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LebrA.19512.a.B1.GE40861	full length		1	682
LebrA.19512.a.B2.GE40862	5'-3' exonuclease		29	652

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	LbrM.03.0520
RefSeq:	XP_001561642.2
UniProt:	A4H3M3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MYAITRHLCY VAGIGCISSS GGSHGTGSHY RNSTQLADAF QSVQQRNQWP FDHVLIDVSF LANSLGYLTR DLVGHERDRE TVKNIHRQLH TVVLRRLQAR KSLALLLDGS EPLWMLEHRR LFPGRRYDSR VYRSCASPMP YLLEEKLRGT AMEQRTPPSE AVISGPATPG LAEGKMSAYL LDLATRIARP PTFPPHLCTP VTANDTICLV GGPELAWMGV GMTPFHNITS VTLQQGELKS CSLLESMEWM RLDHLLKEAP EQTHGNSDGG AALTLQRRLT AVRTDLVLLY LLTNGHPTTG LPQVLAAPFA DVLDAYVDME KEQHSEAALS TPQNALRFRS VLFDEEPVTG AHLGRPALRL RLVALQRLLV RIVRALLGPH VGLPAGNRPT SHAATLLEMA LQTHGLLCSG GVPSQAWSPT PDPAAASSKS GAPTVLNGTV LLDKFPRLSA EMLLQHVTYL LSHEAKPHTG NSADGAATFA PSAKEDSDSA YLSPQHTRHF GLTGVETLLL TATQADQVNQ ILPLYARGHT LPDGVAEDIA QTRNIHEAVR KTKRVLSRVL EQASQELARQ SATGADGESN STAAAVDAAG NNGPHPALTH LPSFLFVRTA GARGPPPGWA YYGVHLGIKA EAMSMRSNLN ASDTTAQRVM ASSSSDNNSN GEVTAAAGSD VGAESARNAS VN

NT Sequence

atgtacgcca ttacacggca cttgtgctac gtcgccggca taggctgcat cagcagcagc ggtggctccc acggtaccgg aagtcactat cgtaactcca cccaactcgc cgacgccttt cagtcggtgc agcagcgcaa ccaatggccc ttcgatcacg ttctcatcga cgtctccttc ctcgccaact ccctcggcta cctcacgcgc gacctggtcg gccacgaacg cgaccgcgaa acggtgaaga acatccacag acagctgcac acggtcgtcc tgcgccgcct ccaagcgcgc aagagcctcg cactgctgct ggatggcagc gagccactct ggatgctcga acatcggcgc ctcttccctg gccgacggta cgactccagg gtctaccgca gctgtgcctc gccgatgccc tacctgctgg aggagaaact ccgcggaact gccatggagc agcgcacccc gccctcggaa gccgtcatca gcggcccagc cacccccgga ctggcagagg gcaagatgag cgcttatctg ctggaccttg ctactcgcat tgctcggcca ccgacattcc cgccgcacct ctgcaccccg gtcacggcga acgacaccat ctgcctggtt ggtgggcccg agctggcttg gatgggtgtt ggtatgacgc catttcacaa catcacaagc gtgacactgc agcaggggga gctgaagagc tgctcgctgc tggagtcgat ggagtggatg cggctggatc acctgctgaa ggaggcgcca gagcagacac atggcaacag cgacggcggc gcggcgttga cgctacagcg gcgcctgacc gctgtccgta ccgacctcgt cctgctctac ctgctgacca atgggcaccc cactacagga ctcccgcaag tgctggcagc cccctttgcc gacgtgctgg acgcgtacgt ggacatggag aaggagcagc acagtgaggc agcactgagc acgccgcaga acgcactgcg ctttcgaagc gtgttgttcg acgaagaacc tgtcaccggc gcgcatctag ggcggccggc gctgcgactc cgcttggtgg cccttcagcg gctgctcgtc cgcatcgtga gggcgttgtt gggaccacac gttggtcttc cggccggcaa ccgtcccacc tcccacgcgg ccacgctgct ggagatggcg ctgcagacac atgggttgct ctgctccggc ggcgtgccgt ctcaagcgtg gagcccaaca cccgatccgg cagctgcctc gagtaagagc ggcgccccca ccgtcctgaa cggcactgtg ctcctcgaca agttccccag gttgtctgcg gagatgctcc tacaacacgt gacctacctc ctcagccacg aagcgaagcc gcatacgggg aactcggcgg atggcgccgc cacttttgct cccagtgcta aggaggacag cgacagcgcg tacctgagcc cgcagcacac acggcacttt gggctgaccg gtgtcgagac gctcctcctc actgcaacgc aggcggacca ggtgaaccaa atcctgcctc tctacgctcg cggccacacc cttccggacg gcgtggccga ggatatcgca cagacgcgca acatccatga ggccgtgcgc aagacgaagc gggtgctgag tcgagtgctg gaacaggcca gccaggagct cgccaggcaa tctgccaccg gcgccgacgg agaaagcaac agcaccgccg ccgcagtcga tgctgctggc aacaatgggc cacaccccgc tctcactcac cttccgtcgt tcttgtttgt gcgcaccgcc ggtgctcgcg ggccaccgcc ggggtgggcg tactacggcg ttcacctcgg catcaaggcg gaggccatga gcatgcggtc caacctcaac gcatccgaca cgacagcgca gcgcgtcatg gcgagcagca gtagcgacaa taacagcaac ggcgaagtca ccgcagctgc tggaagcgat gtcggtgcgg agtcggcccg caacgccagt gttaactga

Details for LebrA.19512.a.B1.GE40861

HARVESTED ON:	12/5/2016
SEQUENCED ON:	12/6/2016
EXPECTED MW:	74kDa
OBSERVED MW:	74kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMY AITRHLCYVA GIGCISSSGG SHGTGSHYRN STQLADAFQS VQQRNQWPFD HVLIDVSFLA NSLGYLTRDL VGHERDRETV KNIHRQLHTV VLRRLQARKS LALLLDGSEP LWMLEHRRLF PGRRYDSRVY RSCASPMPYL LEEKLRGTAM EQRTPPSEAV ISGPATPGLA EGKMSAYLLD LATRIARPPT FPPHLCTPVT ANDTICLVGG PELAWMGVGM TPFHNITSVT LQQGELKSCS LLESMEWMRL DHLLKEAPEQ THGNSDGGAA LTLQRRLTAV RTDLVLLYLL TNGHPTTGLP QVLAAPFADV LDAYVDMEKE QHSEAALSTP QNALRFRSVL FDEEPVTGAH LGRPALRLRL VALQRLLVRI VRALLGPHVG LPAGNRPTSH AATLLEMALQ THGLLCSGGV PSQAWSPTPD PAAASSKSGA PTVLNGTVLL DKFPRLSAEM LLQHVTYLLS HEAKPHTGNS ADGAATFAPS AKEDSDSAYL SPQHTRHFGL TGVETLLLTA TQADQVNQIL PLYARGHTLP DGVAEDIAQT RNIHEAVRKT KRVLSRVLEQ ASQELARQSA TGADGESNST AAAVDAAGNN GPHPALTHLP SFLFVRTAGA RGPPPGWAYY GVHLGIKAEA MSMRSNLNAS DTTAQRVMAS SSSDNNSNGE VTAAAGSDVG AESARNASVN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtacg ccattacacg gcacttgtgc tacgtcgccg gcataggctg catcagcagc agcggtggct cccacggtac cggaagtcac tatcgtaact ccacccaact cgccgacgcc tttcagtcgg tgcagcagcg caaccaatgg cccttcgatc acgttctcat cgacgtctcc ttcctcgcca actccctcgg ctacctcacg cgcgacctgg tcggccacga acgcgaccgc gaaacggtga agaacatcca cagacagctg cacacggtcg tcctgcgccg cctccaagcg cgcaagagcc tcgcactgct gctggatggc agcgagccac tctggatgct cgaacatcgg cgcctcttcc ctggccgacg gtacgactcc agggtctacc gcagctgtgc ctcgccgatg ccctacctgc tggaggagaa actccgcgga actgccatgg agcagcgcac cccgccctcg gaagccgtca tcagcggccc agccaccccc ggactggcag agggcaagat gagcgcttat ctgctggacc ttgctactcg cattgctcgg ccaccgacat tcccgccgca cctctgcacc ccggtcacgg cgaacgacac catctgcctg gttggtgggc ccgagctggc ttggatgggt gttggtatga cgccatttca caacatcaca agcgtgacac tgcagcaggg ggagctgaag agctgctcgc tgctggagtc gatggagtgg atgcggctgg atcacctgct gaaggaggcg ccagagcaga cacatggcaa cagcgacggc ggcgcggcgt tgacgctaca gcggcgcctg accgctgtcc gtaccgacct cgtcctgctc tacctgctga ccaatgggca ccccactaca ggactcccgc aagtgctggc agcccccttt gccgacgtgc tggacgcgta cgtggacatg gagaaggagc agcacagtga ggcagcactg agcacgccgc agaacgcact gcgctttcga agcgtgttgt tcgacgaaga acctgtcacc ggcgcgcatc tagggcggcc ggcgctgcga ctccgcttgg tggcccttca gcggctgctc gtccgcatcg tgagggcgtt gttgggacca cacgttggtc ttccggccgg caaccgtccc acctcccacg cggccacgct gctggagatg gcgctgcaga cacatgggtt gctctgctcc ggcggcgtgc cgtctcaagc gtggagccca acacccgatc cggcagctgc ctcgagtaag agcggcgccc ccaccgtcct gaacggcact gtgctcctcg acaagttccc caggttgtct gcggagatgc tcctacaaca cgtgacctac ctcctcagcc acgaagcgaa gccgcatacg gggaactcgg cggatggcgc cgccactttt gctcccagtg ctaaggagga cagcgacagc gcgtacctga gcccgcagca cacacggcac tttgggctga ccggtgtcga gacgctcctc ctcactgcaa cgcaggcgga ccaggtgaac caaatcctgc ctctctacgc tcgcggccac acccttccgg acggcgtggc cgaggatatc gcacagacgc gcaacatcca tgaggccgtg cgcaagacga agcgggtgct gagtcgagtg ctggaacagg ccagccagga gctcgccagg caatctgcca ccggcgccga cggagaaagc aacagcaccg ccgccgcagt cgatgctgct ggcaacaatg ggccacaccc cgctctcact caccttccgt cgttcttgtt tgtgcgcacc gccggtgctc gcgggccacc gccggggtgg gcgtactacg gcgttcacct cggcatcaag gcggaggcca tgagcatgcg gtccaacctc aacgcatccg acacgacagc gcagcgcgtc atggcgagca gcagtagcga caataacagc aacggcgaag tcaccgcagc tgctggaagc gatgtcggtg cggagtcggc ccgcaacgcc agtgttaact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for LebrA.19512.a.B2.GE40862

HARVESTED ON:	12/5/2016
SEQUENCED ON:	12/6/2016
EXPECTED MW:	69kDa
OBSERVED MW:	69kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHHY RNSTQLADAF QSVQQRNQWP FDHVLIDVSF LANSLGYLTR DLVGHERDRE TVKNIHRQLH TVVLRRLQAR KSLALLLDGS EPLWMLEHRR LFPGRRYDSR VYRSCASPMP YLLEEKLRGT AMEQRTPPSE AVISGPATPG LAEGKMSAYL LDLATRIARP PTFPPHLCTP VTANDTICLV GGPELAWMGV GMTPFHNITS VTLQQGELKS CSLLESMEWM RLDHLLKEAP EQTHGNSDGG AALTLQRRLT AVRTDLVLLY LLTNGHPTTG LPQVLAAPFA DVLDAYVDME KEQHSEAALS TPQNALRFRS VLFDEEPVTG AHLGRPALRL RLVALQRLLV RIVRALLGPH VGLPAGNRPT SHAATLLEMA LQTHGLLCSG GVPSQAWSPT PDPAAASSKS GAPTVLNGTV LLDKFPRLSA EMLLQHVTYL LSHEAKPHTG NSADGAATFA PSAKEDSDSA YLSPQHTRHF GLTGVETLLL TATQADQVNQ ILPLYARGHT LPDGVAEDIA QTRNIHEAVR KTKRVLSRVL EQASQELARQ SATGADGESN STAAAVDAAG NNGPHPALTH LPSFLFVRTA GARGPPPGWA YYGVHLGIKA EAMSMRSNLN ASDTTAQRVM AS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcactatc gtaactccac ccaactcgcc gacgcctttc agtcggtgca gcagcgcaac caatggccct tcgatcacgt tctcatcgac gtctccttcc tcgccaactc cctcggctac ctcacgcgcg acctggtcgg ccacgaacgc gaccgcgaaa cggtgaagaa catccacaga cagctgcaca cggtcgtcct gcgccgcctc caagcgcgca agagcctcgc actgctgctg gatggcagcg agccactctg gatgctcgaa catcggcgcc tcttccctgg ccgacggtac gactccaggg tctaccgcag ctgtgcctcg ccgatgccct acctgctgga ggagaaactc cgcggaactg ccatggagca gcgcaccccg ccctcggaag ccgtcatcag cggcccagcc acccccggac tggcagaggg caagatgagc gcttatctgc tggaccttgc tactcgcatt gctcggccac cgacattccc gccgcacctc tgcaccccgg tcacggcgaa cgacaccatc tgcctggttg gtgggcccga gctggcttgg atgggtgttg gtatgacgcc atttcacaac atcacaagcg tgacactgca gcagggggag ctgaagagct gctcgctgct ggagtcgatg gagtggatgc ggctggatca cctgctgaag gaggcgccag agcagacaca tggcaacagc gacggcggcg cggcgttgac gctacagcgg cgcctgaccg ctgtccgtac cgacctcgtc ctgctctacc tgctgaccaa tgggcacccc actacaggac tcccgcaagt gctggcagcc ccctttgccg acgtgctgga cgcgtacgtg gacatggaga aggagcagca cagtgaggca gcactgagca cgccgcagaa cgcactgcgc tttcgaagcg tgttgttcga cgaagaacct gtcaccggcg cgcatctagg gcggccggcg ctgcgactcc gcttggtggc ccttcagcgg ctgctcgtcc gcatcgtgag ggcgttgttg ggaccacacg ttggtcttcc ggccggcaac cgtcccacct cccacgcggc cacgctgctg gagatggcgc tgcagacaca tgggttgctc tgctccggcg gcgtgccgtc tcaagcgtgg agcccaacac ccgatccggc agctgcctcg agtaagagcg gcgcccccac cgtcctgaac ggcactgtgc tcctcgacaa gttccccagg ttgtctgcgg agatgctcct acaacacgtg acctacctcc tcagccacga agcgaagccg catacgggga actcggcgga tggcgccgcc acttttgctc ccagtgctaa ggaggacagc gacagcgcgt acctgagccc gcagcacaca cggcactttg ggctgaccgg tgtcgagacg ctcctcctca ctgcaacgca ggcggaccag gtgaaccaaa tcctgcctct ctacgctcgc ggccacaccc ttccggacgg cgtggccgag gatatcgcac agacgcgcaa catccatgag gccgtgcgca agacgaagcg ggtgctgagt cgagtgctgg aacaggccag ccaggagctc gccaggcaat ctgccaccgg cgccgacgga gaaagcaaca gcaccgccgc cgcagtcgat gctgctggca acaatgggcc acaccccgct ctcactcacc ttccgtcgtt cttgtttgtg cgcaccgccg gtgctcgcgg gccaccgccg gggtgggcgt actacggcgt tcacctcggc atcaaggcgg aggccatgag catgcggtcc aacctcaacg catccgacac gacagcgcag cgcgtcatgg cgagctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat