LebrA.19511.a

KREN3

CENTER ID: LebrA.19511.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.19511.a.B2.GE41026 RNase III-like catalytic-like domain 219 492
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: LbrM.35.1040
RefSeq: XP_003723237.1
UniProt: E9AIX5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQLRRTSAWR ALCRIPRAVC IQCCESVSVA CTSSSRPTPC EPTKRFSSPM VQEWWVNAAQ EAVISRKAIM RAERIVTEAD DPRSNKRPFY DSDTRRCLVC DTRLEGSYST HSHTVDHIPR VALLKRTISM ILTFLEQASS SPSTPPQALP EKAGSLAGSK RLLKSALPHT ATATAPAPSC FHTLYAESPL FGEDGVPSRG LLYHRPHTLR EFDLVDVIMR RWWNTLHNPP PKRGSLSFDR LLSLSSTELQ MRRWRVRYLL YFLKSRGVLR VSMSVRGDDM SAVAPSSERI HRGEAFERLE MVGDSFFKSL TPDRMHAVFP ADEGGLTTGL QYFERTLDSN HGLLAIYDYL GLDDIIGCYL ANNKAKADVV EAIIGELKVL LWSADVVWGM EYYAVPGERA TLVYLRALVA HTIAELGHTV LMWQLESTLR NCREFILKHT VEGYLTANGA REHRAKGEES EFEFVADLPK YAPLPPLLAW ERQRPGASVP KGEVQYATKQ LLARAPPSPQ DPLRRLAPTA SCIPEGIRKH YAVGQKKWNP NMCAAAVEAL CDSLTLSALP RNSGRTADAL SSDPQSGSLP LYPEAQPSKT PPASRDVRRH VLPALVTANC IVICTTNLPL ATTM
NT Sequence
atgcagcttc gccgcacgtc cgcatggcga gcgctttgcc gtattcctcg tgccgtatgt atacagtgct gtgagtcagt ttcggtggca tgtacgtcgt cgtcgcggcc aacaccatgt gagccgacga agcgtttctc cagcccgatg gtgcaagagt ggtgggtgaa cgctgcccag gaggcagtca tctcccgaaa agccatcatg cgagcagagc gcattgttac ggaggccgac gatccgcgct ccaacaaacg gcccttctat gacagtgata ccagacgctg cctcgtgtgt gacacccgtc tggagggcag ctacagcacg catagccaca ctgttgacca cataccacgc gttgcgctgc tgaaacgcac aatcagtatg attctcacat ttctcgagca ggcctcgtcc tcaccgtcga cgccgccgca ggccttgccg gagaaggctg gttcactggc tggcagtaaa cgcttgctga agtcggcgtt gcctcacaca gcaacggcca ccgcccctgc cccttcttgc ttccacaccc tgtatgccga gtcaccgctc tttggcgagg atggcgtgcc tagccgcggt ctcctctacc accgcccgca cacgctgcgc gagttcgacc tggtagacgt aatcatgcga cgttggtgga acaccctgca taatccacca cctaagcggg gtagtctttc gtttgaccgg ctcctttcgc tgtcctcgac ggagctgcag atgcgacgat ggcgcgtgcg ctatctactc tactttctca agtcccgcgg tgttctgcgc gtttctatgt cagtgagagg cgacgatatg agtgcggtgg cgccttccag cgagcggatc caccgtggag aagcctttga gcgtctcgag atggttggcg actctttctt caagagtctc acccccgacc gtatgcacgc cgtcttccca gctgatgagg gtggactcac gactggtctc cagtattttg agcggacgtt ggattcgaat catggtctgc tggccatcta cgactacctt gggctggacg acataatcgg ctgctatcta gccaacaaca aggcaaaggc cgacgttgta gaggcaatta ttggtgaact gaaggtccta ttgtggtcgg ccgatgtggt gtggggcatg gagtactacg ccgtacctgg ggagcgcgct acgctggtgt acctccgagc actggtggca cacaccatcg ccgagctggg gcacacggtg ctgatgtggc agttggagag cacactgcgc aattgtcgag agtttattct caagcatact gttgaggggt atttgaccgc caacggggcg agagagcatc gagctaaggg cgaagagagc gaattcgaat tcgttgcgga tcttcccaaa tacgcaccgc tgccgccgtt gctcgcgtgg gagcgacaac gcccgggcgc gtcagtgccg aagggtgagg tgcagtacgc cactaagcag ttgctagcca gagcaccacc ttctccccag gatccgcttc gcagactcgc cccgaccgcg agctgcatcc cagaaggtat tcggaagcac tacgccgtag ggcagaaaaa atggaaccca aatatgtgtg ccgcagccgt tgaagccctc tgtgactccc tcacactatc tgcactgcca cgcaacagcg gccgcaccgc ggacgctcta tcgtctgacc cccagtcggg atcgttgccc ctttatccag aagcgcagcc gtccaagacc ccgccggcgt ccagggacgt acgtcgccat gtactgccag ctctggtgac tgcgaattgc atagtaattt gtactaccaa cctgcctctt gctactacca tgtag
Details for LebrA.19511.a.B2.GE41026
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMR RWWNTLHNPP PKRGSLSFDR LLSLSSTELQ MRRWRVRYLL YFLKSRGVLR VSMSVKGDDM SAVAHSSERI HRGEAFERLE MVGDSFFKSL TPDRMHAVFP ADEGGLTTGL QYFERTLDSN HGLLAIYDYL GLDDIIGCYL ANNKAKADVV EAIIGELKVL LWSADVVWGM EYYAVPGERA TLVYLRALVA HTIAELGHTV LMWQLESTLR NCREFILKHT VEGYLTANEA REHRAKGEES EFEFVADLPK YAPLPPLLAW ERQR
Validated NT Sequence
gggcgttgtc gctcccacgc gagcaacggc ggcagcggtg cgtatttggg aagatccgca acgaattcga attcgctctc ttcgccctta gctcgatgct ctctcgcctc gttggcggtc aaatacccct caacagtatg cttgagaata aactctcgac aattgcgcag tgtgctctcc aactgccaca tcagcaccgt gtgccccagc tcggcgatgg tgtgcgccac cagtgctcgg aggtatacca gcgtagcgcg ctccccaggt acggcgtagt actccatgcc ccacaccaca tcggccgacc acaataggac cttcagttca ccaataattg cctctacaac gtcggccttt gccttgttgt tggctagata gcagccgatt atgtcgtcca gcccaaggta gtcgtagatg gccagcagac catgattcga atccaacgtc cgctcaaaat actggagacc agtcgtgagt ccaccctcat cagctgggaa gacggcgtgc atacggtcgg gggtgagact cttgaagaaa gagtcgccaa ccatctcgag acgctcaaag gcttctccac ggtggatccg ctcgctggaa tgcgccaccg cactcatatc gtcgcctttc actgacatag aaacgcgcag aacaccgcgg gacttgagaa agtagagtag atagcgcacg cgccatcgtc gcatctgcag ctccgtcgag gacagcgaaa ggagccggtc aaacgaaaga ctaccccgct taggtggtgg attatgcagg gtgttccacc aacgtcgcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMR RWWNTLHNPP PKRGSLSFDR LLSLSSTELQ MRRWRVRYLL YFLKSRGVLR VSMSVRGDDM SAVAPSSERI HRGEAFERLE MVGDSFFKSL TPDRMHAVFP ADEGGLTTGL QYFERTLDSN HGLLAIYDYL GLDDIIGCYL ANNKAKADVV EAIIGELKVL LWSADVVWGM EYYAVPGERA TLVYLRALVA HTIAELGHTV LMWQLESTLR NCREFILKHT VEGYLTANGA REHRAKGEES EFEFVADLPK YAPLPPLLAW ERQRPGASVP KG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgac gttggtggaa caccctgcat aatccaccac ctaagcgggg tagtctttcg tttgaccggc tcctttcgct gtcctcgacg gagctgcaga tgcgacgatg gcgcgtgcgc tatctactct actttctcaa gtcccgcggt gttctgcgcg tttctatgtc agtgagaggc gacgatatga gtgcggtggc gccttccagc gagcggatcc accgtggaga agcctttgag cgtctcgaga tggttggcga ctctttcttc aagagtctca cccccgaccg tatgcacgcc gtcttcccag ctgatgaggg tggactcacg actggtctcc agtattttga gcggacgttg gattcgaatc atggtctgct ggccatctac gactaccttg ggctggacga cataatcggc tgctatctag ccaacaacaa ggcaaaggcc gacgttgtag aggcaattat tggtgaactg aaggtcctat tgtggtcggc cgatgtggtg tggggcatgg agtactacgc cgtacctggg gagcgcgcta cgctggtgta cctccgagca ctggtggcac acaccatcgc cgagctgggg cacacggtgc tgatgtggca gttggagagc acactgcgca attgtcgaga gtttattctc aagcatactg ttgaggggta tttgaccgcc aacggggcga gagagcatcg agctaagggc gaagagagcg aattcgaatt cgttgcggat cttcccaaat acgcaccgct gccgccgttg ctcgcgtggg agcgacaacg cccgggcgcg tcagtgccga agggttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat