LebrA.19509.a

KREPA2

CENTER ID: LebrA.19509.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.19509.a.B2.GE40860 C2H2 zinc finger and OB fold 442 623
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: LbrM.35.7300
RefSeq: XP_001569339.1
UniProt: A4HQL4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQRRVGVLTA AAACRCALRR LRHVWARPVH ESGALASVNG ASNAGLVEAV RNQSTIPDRR FHCSVCKKSF RLEMAAKLHL QQVHNSEGTV EAGAGPGQGE KEAQQTPVGV FRNAPPQAPT VVSPIVPDTH ERSARRAKPA PKPLHQAERE VPGLVMEKML SVWDDTGLKR MGNQFIHSTM VMRVFAARPS DCGEPLYGAV IPEGENPFED CQYAADKAGP IMANDEKANF YHITESDAFA MASDESLGPL RPSRCPNPFL RKATKDIPKT ATRMEFAEQQ TAPVTPFGQL PMFGQTLPPA TAPMPSAAEQ PSTKDNTVVS SPFAAGASDS PFAGTTNVLS VGQGHSPFGS VEVPADVAAV EPGNAASSVT SSSIPTSPFA AAAGAIPPSP FAAAPSPFTT SSFAAASYAA VEAVAATSSA FVSDGASPVS FSACPASASA KEAPTSTHVC TICNKSFFMH DGLRMHSKAK HGKELPKASR KEKREVPDLP AFIPSPVDLT MTSPFGSSAQ RSVWTEVELT PYAQSVSNIT VAGRVLDVEV SNEGALLLSL YVSDSAEKEA EVIPVRCTSD ALRTLGKTLE QGDHVFACGS LRVLPFTDKK TQKTYSSAVV HVTFPTGIIA KLS
NT Sequence
atgcagaggc gagtcggtgt cctaactgct gctgctgcgt gccgctgcgc tctgcgccga ctgcgccacg tatgggctcg ccccgtacac gaatcgggcg ctctggcctc cgtgaacggc gcctcaaatg ccggtctggt ggaagctgtg cggaatcaga gtaccatccc cgaccggcgc tttcactgta gcgtttgcaa gaagtctttt cgcttggaga tggcggcaaa actgcacctt caacaggtgc acaacagcga gggcaccgtg gaggcaggcg ctggtcccgg gcagggcgag aaggaagcgc agcagacccc ggtaggtgtg tttcgtaacg cccctccgca ggcgccgact gtcgtctcgc caatcgtgcc cgatactcac gagcgctctg cgcgccgtgc gaagccggcc ccaaaaccac tccaccaggc cgagcgcgag gtgccaggtc tcgtgatgga gaagatgctc tcggtatggg atgacacggg cctgaagcga atgggtaatc agtttattca cagtacgatg gtaatgcgtg tgtttgctgc tcgcccgtct gactgcggcg agccgttgta cggagccgtg attccagaag gtgagaaccc gtttgaggat tgccaatatg ccgctgacaa ggcaggccct atcatggcga atgacgagaa agccaatttt tatcacatta ctgaaagcga cgcttttgca atggcgtctg atgagtctct cggtccattg cgcccctctc ggtgcccgaa cccgtttctc cgaaaagcaa caaaggatat tccgaaaact gccacgagga tggagttcgc agagcagcaa acagccccgg taacgccatt cggccagctc cccatgtttg ggcagactct tccacctgct acggcaccca tgcccagcgc cgccgagcaa ccgtcgacga aggacaacac tgtcgtttct tctcccttcg ccgccggcgc cagtgactca ccgtttgcgg ggacgacaaa cgttctgtct gtcgggcaag ggcactctcc gtttggctcg gtcgaggttc ctgcagatgt ggccgctgtc gagcctggga atgcagcgtc ctctgtgact tcctcttcga taccgacgtc gccgttcgcg gctgctgctg gtgcgatccc tccgtctcca tttgccgcag ccccgagccc cttcaccacc agctcttttg cagccgcctc gtatgcagca gtggaggcag tcgcggccac aagcagcgct tttgtttctg acggcgcatc ccctgtctcc ttctccgcgt gtcccgccag cgcatcggct aaggaggcgc ccacctcgac gcatgtgtgc accatctgca ataagagctt ttttatgcat gacgggcttc gaatgcacag caaggccaag catgggaagg agctgccgaa ggcgtcacgg aaggagaagc gcgaagtgcc ggatttacca gccttcatcc ccagcccggt ggatctcacg atgacatccc ccttcggctc gtctgcacag cgatcggtgt ggacggaagt ggaactcaca ccctatgcgc agtccgtgag taacattact gtggcaggtc gcgtgctgga tgtcgaagta tcgaatgaag gggcgttgct cttgtccctt tatgtctccg attcggccga aaaggaggct gaggtgattc ctgttcgctg tacctcagat gccttgagaa ccttgggcaa aacgctcgag caaggcgacc acgtattcgc gtgtggctcg ttgcgcgttt tgcccttcac tgataagaaa acgcaaaaga cgtattcctc cgccgtcgtg cacgttactt ttccgacggg aattattgcc aagctgagct ga
Details for LebrA.19509.a.B2.GE40860
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 21kDa
OBSERVED MW: 20kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME APTSTHVCTI CNKSFFMHDG LRMHSKAKHG KELPKASRKE KREVPDLPAF IPSPVDLTMT SPFGSSAQRS VWTEVELTPY AQSVSNITVA GRVLDVEVSN EGALLLSLYV SDSAEKEAEV IPVRCTSDAL RTLGKTLEQG DHIFACGSLR ILPFTDKKTQ KTYSSAVVHV TFPTGIIAKL S
Validated NT Sequence
atcttactca cttagctcag cttggcaata attcccgtcg gaaaagtaac gtgcacgacg gcggaggaat acgtcttttg cgttttctta tcagtgaagg gcaaaatgcg caacgagcca cacgcgaata tgtggtcgcc ttgctcgagc gttttgccca aggttctcaa ggcatctgag gtacagcgaa caggaatcac ttcagcctcc ttttcggccg aatcggagac ataaagggac aagagcaacg ccccttcatt cgatacttcg acatccagta cgcgacctgc cacagtaatg ttactcacgg actgtgcata gggtgtgagt tccacttccg tccacaccga tcgctgtgca gacgagccga agggggatgt catcgtgaga tccaccgggc tggggatgaa ggctggtaaa tccggcactt cgcgcttctc cttccgtgac gccttcggga gctccttccc atgcttggcc ttgctgtgca ttcgaagccc atcatgcata aaaaagctct tattgcagat ggtgcacaca tgcgtcgagg tgggcgcctc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHEA PTSTHVCTIC NKSFFMHDGL RMHSKAKHGK ELPKASRKEK REVPDLPAFI PSPVDLTMTS PFGSSAQRSV WTEVELTPYA QSVSNITVAG RVLDVEVSNE GALLLSLYVS DSAEKEAEVI PVRCTSDALR TLGKTLEQGD HVFACGSLRV LPFTDKKTQK TYSSAVVHVT FPTGIIAKLS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaggcgc ccacctcgac gcatgtgtgc accatctgca ataagagctt ttttatgcat gacgggcttc gaatgcacag caaggccaag catgggaagg agctgccgaa ggcgtcacgg aaggagaagc gcgaagtgcc ggatttacca gccttcatcc ccagcccggt ggatctcacg atgacatccc ccttcggctc gtctgcacag cgatcggtgt ggacggaagt ggaactcaca ccctatgcgc agtccgtgag taacattact gtggcaggtc gcgtgctgga tgtcgaagta tcgaatgaag gggcgttgct cttgtccctt tatgtctccg attcggccga aaaggaggct gaggtgattc ctgttcgctg tacctcagat gccttgagaa ccttgggcaa aacgctcgag caaggcgacc acgtattcgc gtgtggctcg ttgcgcgttt tgcccttcac tgataagaaa acgcaaaaga cgtattcctc cgccgtcgtg cacgttactt ttccgacggg aattattgcc aagctgagct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat