LebrA.19506.a

KREPB6

CENTER ID: LebrA.19506.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.19506.a.B1.GE40859 full length 1 539
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: LbrM.29.1520
RefSeq: XP_001566496.1
UniProt: A4HHF5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATPVRFTKV SASAPRWSWV AADVAENLVR LQGVMHRATP RRLPLQLLHG QARQTATVTK LSGVSRCLST AAPRTGIFSV AGSRGSSPSP KQDPPAPSRK SVHSMSLTLS AESVWETVPH WCELCSEPVN SWHSHMGKRD HMCLEMILNT MVSSKGLWEP DKVWDSIELF RGHYTKRPRG QPRTPSPTVQ RLLKEGLYVP SPDHPLSIFY DAFDRGDPYA RREELHLLLL YLNECGMLCL TPDLFPPAVF QGHLLLHSEV VPVLTRIFPD GEVRGISALL QMITCTYGLA AAYEMCGMER LVPVSLLQHR GVNPELMSAL SSVEEFDSFA ASDDATGGIG GEEALGESAE GETIDSPLST DQSMAMRSLS AAMPRAILGS LRWALAPEDA SPPPAIFQDH KRNAYLTTLA ARAARLLMSE LIFERISEYV HRLEWFLRTD IGREWLHSHH DGHREADAGS SRAASPRWLR TQCITPSLSN EGNVAYTNGG LVAHGLNGTA ASECTVQMHD AARKARMHGR RGLPRAPANS PLARSVRSL
NT Sequence
atggcgaccc ctgttcgatt caccaaggta agcgcgagtg ccccacggtg gtcctgggtg gctgccgacg tggcagagaa cctcgtgcgc ctgcagggtg tgatgcaccg cgctacgcca cgacgattac cgctgcagtt gctacacggg caggctaggc agacggcaac agtgaccaag ctgtcaggtg tgtctcgctg tctgtctacg gccgcaccga ggactgggat cttctcagtt gctggatcac ggggctcgtc cccgtcaccc aaacaggacc cgcctgctcc ctcccgaaag tcggtgcact ccatgagcct cacactatcg gcggaatcgg tgtgggaaac ggtgccacac tggtgcgagc tgtgctctga gcccgtcaat agctggcaca gccacatggg caagcgcgac cacatgtgtc tcgagatgat cctgaacacc atggtcagca gcaaaggact ctgggagccg gacaaggtgt gggacagcat tgagctgttt agaggtcact acacaaagcg cccacgcgga cagccacgaa cgccctcgcc gactgtacaa cgcctgctga aggagggcct ctacgtgccg agccctgatc acccgctcag catcttctac gatgccttcg accggggcga cccatacgcg cgacgagagg agctgcatct gctcctcttg tacttgaacg agtgtgggat gctctgtctc acccccgatc tgttccctcc agcggtcttt caaggacact tactcttgca cagcgaggta gtgccggttc tgacgcgcat cttccccgac ggggaggtgc gcggtatctc ggcgttgttg cagatgataa cctgcaccta tggactggca gcagcctatg aaatgtgtgg gatggagcgc ttggtgccgg tctccttgct gcagcaccga ggagtgaacc cagagctaat gtctgccttg tcgtccgtag aagagttcga cagttttgcg gcaagtgatg atgcaacagg tggcatcgga ggcgaagagg ctctgggaga aagcgcggaa ggtgagacta tcgattcacc cttatcgacg gaccaatcca tggcaatgcg ctccctgagc gccgccatgc cccgtgccat cctcggctcc ctccgctggg ccctcgctcc agaggacgcg agtcccccgc cggccatctt tcaggaccac aagcgcaacg cctacttgac caccttggca gcgcgcgcgg cacgcctgct gatgtccgag ctgatcttcg agcgcatctc tgaatacgta catcgcttag agtggtttct gcggacagac atcgggcggg agtggctgca cagtcaccac gatggccatc gagaggcaga cgcgggcagc agtcgcgcgg cctcgccgcg ctggctgcgc actcagtgca tcaccccttc attgtcgaac gagggcaatg ttgcctatac aaatggtggg ctcgtcgccc atgggcttaa cggcaccgca gcatctgagt gtaccgttca gatgcacgac gcggctcgta aagcgcgcat gcacggtcgc cgcggattgc ccagggcgcc agccaactcc cctttggctc gcagtgtacg gtctctgtga
Details for LebrA.19506.a.B1.GE40859
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 60kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 61
Validated AA Sequence
MAHHHHHHMA TPVRFTKVSA SAPRWSWVAA DVAENLVRLQ GVLHRATPRQ SPLQLLHGQA RQTATVTKLS GVSRCLSTAA PRTGIFSVAG SRGSSPSPKQ DSPAPSRKSV HSMSLTLSAE SVWETVPHWC ELCSEPVNSW HSHMGKRDHM CLEMILNTMV SSKGLWEPDK VWDSIELFRG HYTKRPRGQP RTPSPTVQRL LKEGLYVPSP DHPLSIFYDA FDRGDPYARR EELHLLLLYL NECGMLCLTP DLFPPAVFQG HLLLHSEVVP VLTRIFPDGE VRGISALLQM ITCTYGLA
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA TPVRFTKVSA SAPRWSWVAA DVAENLVRLQ GVMHRATPRR LPLQLLHGQA RQTATVTKLS GVSRCLSTAA PRTGIFSVAG SRGSSPSPKQ DPPAPSRKSV HSMSLTLSAE SVWETVPHWC ELCSEPVNSW HSHMGKRDHM CLEMILNTMV SSKGLWEPDK VWDSIELFRG HYTKRPRGQP RTPSPTVQRL LKEGLYVPSP DHPLSIFYDA FDRGDPYARR EELHLLLLYL NECGMLCLTP DLFPPAVFQG HLLLHSEVVP VLTRIFPDGE VRGISALLQM ITCTYGLAAA YEMCGMERLV PVSLLQHRGV NPELMSALSS VEEFDSFAAS DDATGGIGGE EALGESAEGE TIDSPLSTDQ SMAMRSLSAA MPRAILGSLR WALAPEDASP PPAIFQDHKR NAYLTTLAAR AARLLMSELI FERISEYVHR LEWFLRTDIG REWLHSHHDG HREADAGSSR AASPRWLRTQ CITPSLSNEG NVAYTNGGLV AHGLNGTAAS ECTVQMHDAA RKARMHGRRG LPRAPANSPL ARSVRSL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga cccctgttcg attcaccaag gtaagcgcga gtgccccacg gtggtcctgg gtggctgccg acgtggcaga gaacctcgtg cgcctgcagg gtgtgatgca ccgcgctacg ccacgacgat taccgctgca gttgctacac gggcaggcta ggcagacggc aacagtgacc aagctgtcag gtgtgtctcg ctgtctgtct acggccgcac cgaggactgg gatcttctca gttgctggat cacggggctc gtccccgtca cccaaacagg acccgcctgc tccctcccga aagtcggtgc actccatgag cctcacacta tcggcggaat cggtgtggga aacggtgcca cactggtgcg agctgtgctc tgagcccgtc aatagctggc acagccacat gggcaagcgc gaccacatgt gtctcgagat gatcctgaac accatggtca gcagcaaagg actctgggag ccggacaagg tgtgggacag cattgagctg tttagaggtc actacacaaa gcgcccacgc ggacagccac gaacgccctc gccgactgta caacgcctgc tgaaggaggg cctctacgtg ccgagccctg atcacccgct cagcatcttc tacgatgcct tcgaccgggg cgacccatac gcgcgacgag aggagctgca tctgctcctc ttgtacttga acgagtgtgg gatgctctgt ctcacccccg atctgttccc tccagcggtc tttcaaggac acttactctt gcacagcgag gtagtgccgg ttctgacgcg catcttcccc gacggggagg tgcgcggtat ctcggcgttg ttgcagatga taacctgcac ctatggactg gcagcagcct atgaaatgtg tgggatggag cgcttggtgc cggtctcctt gctgcagcac cgaggagtga acccagagct aatgtctgcc ttgtcgtccg tagaagagtt cgacagtttt gcggcaagtg atgatgcaac aggtggcatc ggaggcgaag aggctctggg agaaagcgcg gaaggtgaga ctatcgattc acccttatcg acggaccaat ccatggcaat gcgctccctg agcgccgcca tgccccgtgc catcctcggc tccctccgct gggccctcgc tccagaggac gcgagtcccc cgccggccat ctttcaggac cacaagcgca acgcctactt gaccaccttg gcagcgcgcg cggcacgcct gctgatgtcc gagctgatct tcgagcgcat ctctgaatac gtacatcgct tagagtggtt tctgcggaca gacatcgggc gggagtggct gcacagtcac cacgatggcc atcgagaggc agacgcgggc agcagtcgcg cggcctcgcc gcgctggctg cgcactcagt gcatcacccc ttcattgtcg aacgagggca atgttgccta tacaaatggt gggctcgtcg cccatgggct taacggcacc gcagcatctg agtgtaccgt tcagatgcac gacgcggctc gtaaagcgcg catgcacggt cgccgcggat tgcccagggc gccagccaac tcccctttgg ctcgcagtgt acggtctctg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat