LebrA.19501.a

JBP3 (glucosyl transferase interacting protein 2) (GTIP2)

CENTER ID: LebrA.19501.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
LebrA.19501.a.B1.GE40857 full length 1 673
LebrA.19501.a.B2.GE40680 C-term domain 395 667
LebrA.19501.a.B3.GE40681 J-binding domain 140 288
LebrA.19501.a.B4.GE41025 N-extended J-binding domain 94 295
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
LebrA.19501.a.B4.PW38193 N-extended J-binding domain 94 295
Molecular models
METHOD RESULTS
comparative modelling Robetta_79394
External Resources
RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.35.0480
UniProt: A4HNP7
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSDRAESTA AGDPRQQSST ASSGSSNASS TSSDEVSSTL SDSSSSSSSS HSSSSSGFES SDSERPSSPP QGQNAVSVGR AAATQQPQQQ QQQQQQQRHV QLNPELRFER PDKEYIPPPP LKDMQDMAKV LFFLSTDKKR YPDGRHRTLD YFRAAVEMFV TEVRQEYQRQ YQQAQRSGRA MQRLTWKNSG ELAICFACCC DNVKLLYDNL QPGPLKPLWD AFVGQLAPML IVQSRVPEAM LSSQTYHIKY MDWVKGGTRY GGEQSAANVR FPSALDRRVK VETYLRSGAG YKIEAVQVGT TSAASAAVPA GVTPRPPLAA AAAPVQATET RAPFAGRLPG TGAVKLPVVP GVGGPRPPVK VAMKHASGGV GPGTVTTATA PRLASTGVKQ MPPEMKRLFW LSVLAHVKGG DASRAFPDDR LEALPGLLRL CEMTEHTEEM CILMDVLAAS SSSVQTVFER LGGLLSFRRF ASHFIQSEEA EPLMQLIEQL ARWRVVAWGS TSQQAWQTDM NQRRTRDLMW LRTLPLIPEA KRPAWGALLL VMEQRYIFKT PRPDDADMEN PLKRRRPEGE SGYLHAMLSD NVDMQRWVVP RVNVFSAIRL PVELRVPPLC TKLEEDTVRF LNSKRDYAEK MQVVWRERLE KILSAQPRAP GAAVSVPLWY DPYALLSVAS PQP
NT Sequence
atgtcctcag accgcgccga gagcacggct gctggcgatc cccgccagca gtcgtcaaca gcttctagcg ggtcgtcgaa tgccagcagt accagctctg acgaggtcag cagcactctc tccgacagca gcagcagcag cagcagtagc cacagcagca gttcctctgg gttcgaatca tctgatagcg agcgcccttc gtccccaccg cagggtcaaa acgccgtttc agtagggaga gccgcggcca cgcaacagcc acagcagcag cagcagcagc agcagcagca gcggcatgtg cagctgaatc ccgaattgcg ctttgagagg ccagacaagg agtacatccc accaccgccc ctcaaggaca tgcaggacat ggctaaggtg ctgtttttct tgagtaccga taagaagcgc tatccggatg ggcgccaccg cacgctggat tacttccgtg ccgcggtgga gatgttcgtg acggaggtac ggcaggagta ccaacggcag tatcaacagg cgcagcgcag cggccgtgcg atgcaacgct taacatggaa gaactcgggg gaactggcga tttgctttgc ctgctgctgc gacaatgtga agcttctgta cgacaacctc cagcccggcc ccctcaaacc gctctgggat gcctttgtgg gccagcttgc gccgatgctc atcgttcaaa gtcgagtgcc agaggcgatg ctgtcgtccc agacgtatca catcaagtac atggactggg ttaagggcgg cacacgttac ggtggcgagc agtcggccgc gaatgtccgt tttccctcag cattggaccg ccgcgtaaag gtcgaaacgt acctacggag cggcgcaggg tacaagatcg aggctgtgca ggtggggacg acatccgccg cgtcggcggc cgtccccgct ggggtgacgc ctcgtcctcc gttggctgct gctgcggctc cagtgcaggc tacagagacc cgagcgccat tcgcggggcg gttacccggc acaggtgcag tgaagttgcc ggtcgttcct ggcgtcggtg gacctcgccc gccagtgaag gtggccatga agcacgctag cggtggtgtt gggcccggca ctgtgacgac tgccaccgct cctcgactcg cttccacagg cgtgaaacag atgccgccgg agatgaagcg tctgttttgg ttgtcggtac tggcgcatgt gaagggcggg gatgcgtccc gggcttttcc ggatgatcga ctggaggccc ttcccggcct tctgcgactg tgcgagatga cagaacacac ggaggagatg tgcatcttga tggacgtgct ggcggcctcc agcagcagcg tccagacagt ctttgagcgc ctaggcggtc ttctgtcgtt ccgccggttc gcctcccact tcatccagag tgaagaggca gagccactca tgcagcttat cgagcaactc gcgcggtggc gtgtcgtcgc atggggcagt acttctcaac aggcgtggca gaccgatatg aaccaacggc gcacacgcga cctgatgtgg ctccgcaccc tgcccctcat cccggaggca aagaggccgg cgtggggcgc gctgctgctg gtgatggagc aacggtacat tttcaagacc ccgcgccctg atgacgcgga catggagaat ccgctaaagc ggaggcggcc cgagggcgag tccggctacc tgcacgctat gctgagtgac aacgtcgaca tgcaaaggtg ggtggtgccc cgagtcaatg tgttctccgc tattcgccta ccagtggagc tgcgtgtgcc gccgctgtgc acaaagttgg aggaagacac cgtgcgcttc ctcaactcca agcgcgacta cgctgagaag atgcaggtgg tatggcgtga gcggctagag aagattctga gcgcgcagcc tcgtgcgccg ggtgcagctg tctcggtgcc tctctggtat gacccgtacg cgctactgag cgtggcgtct ccgcaaccat ga
Details for LebrA.19501.a.B1.GE40857
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 75kDa
OBSERVED MW: 75kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 50
Validated AA Sequence
MAHHHHHHMS SDRAESTAAG APRQQSSTAS SGSSNASSTS SDEVSSTLSN SSSSSSSSSS SHSSSSSGFE SSDSERPSSP PQGQNAVSVG RAAATQQPQQ QQQQQQQQRH VQLNPELRFE RPDKEYIPPP PLKDMQDMAK VLFFLSTDKK RYPDGRHRTL DYFRAAVEMF VTEVRQEYQR QYQQAQRSGR AMQRLTWKNS GELAICFACC CDNVKLLYDN LQPGPLKPLW DAFVGQLAPM LIVQSRVPEA MLSSQTYHIK YMDWVKGGTR YGGEQSAANV RFPSALDRRV KVETYLRSGA XYKXEX
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS SDRAESTAAG DPRQQSSTAS SGSSNASSTS SDEVSSTLSD SSSSSSSSHS SSSSGFESSD SERPSSPPQG QNAVSVGRAA ATQQPQQQQQ QQQQQRHVQL NPELRFERPD KEYIPPPPLK DMQDMAKVLF FLSTDKKRYP DGRHRTLDYF RAAVEMFVTE VRQEYQRQYQ QAQRSGRAMQ RLTWKNSGEL AICFACCCDN VKLLYDNLQP GPLKPLWDAF VGQLAPMLIV QSRVPEAMLS SQTYHIKYMD WVKGGTRYGG EQSAANVRFP SALDRRVKVE TYLRSGAGYK IEAVQVGTTS AASAAVPAGV TPRPPLAAAA APVQATETRA PFAGRLPGTG AVKLPVVPGV GGPRPPVKVA MKHASGGVGP GTVTTATAPR LASTGVKQMP PEMKRLFWLS VLAHVKGGDA SRAFPDDRLE ALPGLLRLCE MTEHTEEMCI LMDVLAASSS SVQTVFERLG GLLSFRRFAS HFIQSEEAEP LMQLIEQLAR WRVVAWGSTS QQAWQTDMNQ RRTRDLMWLR TLPLIPEAKR PAWGALLLVM EQRYIFKTPR PDDADMENPL KRRRPEGESG YLHAMLSDNV DMQRWVVPRV NVFSAIRLPV ELRVPPLCTK LEEDTVRFLN SKRDYAEKMQ VVWRERLEKI LSAQPRAPGA AVSVPLWYDP YALLSVASPQ P
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcct cagaccgcgc cgagagcacg gctgctggcg atccccgcca gcagtcgtca acagcttcta gcgggtcgtc gaatgccagc agtaccagct ctgacgaggt cagcagcact ctctccgaca gcagcagcag cagcagcagt agccacagca gcagttcctc tgggttcgaa tcatctgata gcgagcgccc ttcgtcccca ccgcagggtc aaaacgccgt ttcagtaggg agagccgcgg ccacgcaaca gccacagcag cagcagcagc agcagcagca gcagcggcat gtgcagctga atcccgaatt gcgctttgag aggccagaca aggagtacat cccaccaccg cccctcaagg acatgcagga catggctaag gtgctgtttt tcttgagtac cgataagaag cgctatccgg atgggcgcca ccgcacgctg gattacttcc gtgccgcggt ggagatgttc gtgacggagg tacggcagga gtaccaacgg cagtatcaac aggcgcagcg cagcggccgt gcgatgcaac gcttaacatg gaagaactcg ggggaactgg cgatttgctt tgcctgctgc tgcgacaatg tgaagcttct gtacgacaac ctccagcccg gccccctcaa accgctctgg gatgcctttg tgggccagct tgcgccgatg ctcatcgttc aaagtcgagt gccagaggcg atgctgtcgt cccagacgta tcacatcaag tacatggact gggttaaggg cggcacacgt tacggtggcg agcagtcggc cgcgaatgtc cgttttccct cagcattgga ccgccgcgta aaggtcgaaa cgtacctacg gagcggcgca gggtacaaga tcgaggctgt gcaggtgggg acgacatccg ccgcgtcggc ggccgtcccc gctggggtga cgcctcgtcc tccgttggct gctgctgcgg ctccagtgca ggctacagag acccgagcgc cattcgcggg gcggttaccc ggcacaggtg cagtgaagtt gccggtcgtt cctggcgtcg gtggacctcg cccgccagtg aaggtggcca tgaagcacgc tagcggtggt gttgggcccg gcactgtgac gactgccacc gctcctcgac tcgcttccac aggcgtgaaa cagatgccgc cggagatgaa gcgtctgttt tggttgtcgg tactggcgca tgtgaagggc ggggatgcgt cccgggcttt tccggatgat cgactggagg cccttcccgg ccttctgcga ctgtgcgaga tgacagaaca cacggaggag atgtgcatct tgatggacgt gctggcggcc tccagcagca gcgtccagac agtctttgag cgcctaggcg gtcttctgtc gttccgccgg ttcgcctccc acttcatcca gagtgaagag gcagagccac tcatgcagct tatcgagcaa ctcgcgcggt ggcgtgtcgt cgcatggggc agtacttctc aacaggcgtg gcagaccgat atgaaccaac ggcgcacacg cgacctgatg tggctccgca ccctgcccct catcccggag gcaaagaggc cggcgtgggg cgcgctgctg ctggtgatgg agcaacggta cattttcaag accccgcgcc ctgatgacgc ggacatggag aatccgctaa agcggaggcg gcccgagggc gagtccggct acctgcacgc tatgctgagt gacaacgtcg acatgcaaag gtgggtggtg ccccgagtca atgtgttctc cgctattcgc ctaccagtgg agctgcgtgt gccgccgctg tgcacaaagt tggaggaaga caccgtgcgc ttcctcaact ccaagcgcga ctacgctgag aagatgcagg tggtatggcg tgagcggcta gagaagattc tgagcgcgca gcctcgtgcg ccgggtgcag ctgtctcggt gcctctctgg tatgacccgt acgcgctact gagcgtggcg tctccgcaac catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for LebrA.19501.a.B2.GE40680
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 33kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK RLFWLSVLAH VKGGDASRAF PDDRLEALPG LLRLCEMTEH TEEMCILMDV LAASSSSVQT VFERLGGLLS FRRFASHFIQ SEEAEPLMQL IEQLARWRVV AWGSTSQQAW QTDMNQRRTR DLMWLRTLPL IPEAKRPAWG ALLLVMEQRY IFKAPRPDDA DMENPLKRRR PEGESGYLHA MLSDNVDMQR WVVPRVNVFS AVRLPVELRV PPLCTKLEED TVRFLNSKRD YAEKMQVVWR ERLEKILSAQ PRAPGAAVSV PLWYXXYALL S
Validated NT Sequence
atggctcacc accaccacca ccatatgaag cgtctgtttt ggttgtcggt actggcgcat gtgaagggcg gggatgcgtc ccgggctttt ccggatgatc gactggaggc ccttcccggc cttctgcgac tgtgcgagat gacagaacac acggaggaga tgtgcatctt gatggacgtg ctggcggcct ccagcagcag cgtccagaca gtctttgagc gcctaggcgg tcttctgtcg ttccgccggt tcgcctccca cttcatccag agtgaagagg cagagccact catgcagctt atcgagcaac tcgcgcggtg gcgtgtcgtg gcatggggca gtacttctca gcaggcgtgg cagaccgata tgaaccaacg gcgcacacgc gacctgatgt ggctccgcac cctgcccctc atcccggagg caaagaggcc ggcgtggggc gcgctgctgc tggtgatgga gcaacggtac attttcaagg ccccgcgccc tgatgacgcg gacatggaga atccgctaaa gcggaggcgg cccgagggcg agtccggcta cctgcacgct atgctgagtg acaacgtcga catgcaaagg tgggtggtgc cccgagtcaa tgtgttctcc gctgttcgcc taccagtgga gctgcgtgtg ccgccgctgt gcacaaagtt agaggaagac accgtgcgct tcctcaactc caagcgcgac tatgctgaga agatgcaggt ggtatggcgt gagcggctag agaagattct gagcgcgcag cctcgtgcgc cgggtgcagc tgtctcggtg cctctctggt atgnnnnnta cgcgctactg agctaagtga gtaagat
Expected Protein Sequence
MAHHHHHHMK RLFWLSVLAH VKGGDASRAF PDDRLEALPG LLRLCEMTEH TEEMCILMDV LAASSSSVQT VFERLGGLLS FRRFASHFIQ SEEAEPLMQL IEQLARWRVV AWGSTSQQAW QTDMNQRRTR DLMWLRTLPL IPEAKRPAWG ALLLVMEQRY IFKTPRPDDA DMENPLKRRR PEGESGYLHA MLSDNVDMQR WVVPRVNVFS AIRLPVELRV PPLCTKLEED TVRFLNSKRD YAEKMQVVWR ERLEKILSAQ PRAPGAAVSV PLWYDPYALL S
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc gtctgttttg gttgtcggta ctggcgcatg tgaagggcgg ggatgcgtcc cgggcttttc cggatgatcg actggaggcc cttcccggcc ttctgcgact gtgcgagatg acagaacaca cggaggagat gtgcatcttg atggacgtgc tggcggcctc cagcagcagc gtccagacag tctttgagcg cctaggcggt cttctgtcgt tccgccggtt cgcctcccac ttcatccaga gtgaagaggc agagccactc atgcagctta tcgagcaact cgcgcggtgg cgtgtcgtcg catggggcag tacttctcaa caggcgtggc agaccgatat gaaccaacgg cgcacacgcg acctgatgtg gctccgcacc ctgcccctca tcccggaggc aaagaggccg gcgtggggcg cgctgctgct ggtgatggag caacggtaca ttttcaagac cccgcgccct gatgacgcgg acatggagaa tccgctaaag cggaggcggc ccgagggcga gtccggctac ctgcacgcta tgctgagtga caacgtcgac atgcaaaggt gggtggtgcc ccgagtcaat gtgttctccg ctattcgcct accagtggag ctgcgtgtgc cgccgctgtg cacaaagttg gaggaagaca ccgtgcgctt cctcaactcc aagcgcgact acgctgagaa gatgcaggtg gtatggcgtg agcggctaga gaagattctg agcgcgcagc ctcgtgcgcc gggtgcagct gtctcggtgc ctctctggta tgacccgtac gcgctactga gctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for LebrA.19501.a.B3.GE40681
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 18kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR YPDGRHRTLD YFRAAVEMFV TEVRQEYQRQ YQQAQRSGRA MQRLTWKNSG ELAICFACCC DNVKLLYDNL QPGPLKPLWD AFVGQLAPML IVQSRVPEAM LSSQTYHIKY MDWVKGGTRY GGEQSVANVR FPSALDRRVK VETYLRSG
Validated NT Sequence
atggctcacc accaccacca ccatatgcgc tatccggatg ggcgccaccg cacgctggat tacttccgtg ccgcggtgga gatgtttgtg acggaggtac ggcaggagta ccaacggcag tatcaacagg cgcagcgcag cggtcgtgcg atgcaacgct taacatggaa gaactcgggg gaactggcga tttgctttgc ctgctgctgc gacaatgtga agctgctgta cgacaacctc cagcccggcc ccctcaaacc tctctgggat gcctttgtcg gccagcttgc gccgatgctc atcgttcaaa gtcgagtgcc agaggcgatg ctgtcgtccc agacgtatca catcaagtac atggactggg ttaagggcgg cacacgttac ggtggcgagc agtcggtcgc gaatgtccgt tttccctcag cattggaccg ccgtgtaaag gtcgaaacgt acctacggag cggctaagtg agtaagat
Expected Protein Sequence
MAHHHHHHRY PDGRHRTLDY FRAAVEMFVT EVRQEYQRQY QQAQRSGRAM QRLTWKNSGE LAICFACCCD NVKLLYDNLQ PGPLKPLWDA FVGQLAPMLI VQSRVPEAML SSQTYHIKYM DWVKGGTRYG GEQSAANVRF PSALDRRVKV ETYLRSG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgctatc cggatgggcg ccaccgcacg ctggattact tccgtgccgc ggtggagatg ttcgtgacgg aggtacggca ggagtaccaa cggcagtatc aacaggcgca gcgcagcggc cgtgcgatgc aacgcttaac atggaagaac tcgggggaac tggcgatttg ctttgcctgc tgctgcgaca atgtgaagct tctgtacgac aacctccagc ccggccccct caaaccgctc tgggatgcct ttgtgggcca gcttgcgccg atgctcatcg ttcaaagtcg agtgccagag gcgatgctgt cgtcccagac gtatcacatc aagtacatgg actgggttaa gggcggcaca cgttacggtg gcgagcagtc ggccgcgaat gtccgttttc cctcagcatt ggaccgccgc gtaaaggtcg aaacgtacct acggagcggc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for LebrA.19501.a.B4.GE41025
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 25kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMQ QQQRHVQLNP ELRFERPDKE YIPPPPLKDM QDMAKVLFFL STDKKRYPDG RHRTLDYFRA AVEMFVTEVR QEYQRQYQQA QRSGRAMQRL TWKNSGELAI CFACCCDNVK LLYDNLQPGP LKPLWDAFVG QLAPMLIVQS RVPEAMLSSQ TYHIKYMDWV KGGTRYGGEQ SAANVRFPSA LDRRVKVETY LRSGAGYKIE A
Validated NT Sequence
atcttactca cttaagcctc gatcttgtac cctgcgccgc tccgtaggta cgtttcgacc tttacacggc ggtccaatgc tgagggaaaa cggacattcg cggccgactg ctcgccaccg taacgtgtgc cgcccttaac ccagtccatg tacttgatgt gatacgtctg ggacgacagc atcgcctctg gcactcgact ttgaacgatg agcatcggcg caagctggcc gacaaaggca tcccagagag gtttgagggg gccgggctgg aggttgtcgt acagcagctt cacattgtcg cagcagcagg caaagcaaat cgccagttcc cccgagttct tccatgttaa gcgttgcatc gcacgaccgc tgcgctgcgc ctgttgatac tgccgttggt actcctgccg tacctccgtc acaaacatct ccaccgcggc acggaagtaa tccagcgtgc ggtggcgccc atccggatag cgcttcttat cggtactcaa gaaaaacagc accttagcca tgtcctgcat gtccttgagg ggcggtggtg ggatgtactc cttgtctggc ctctcaaagc gcaattcggg attcagctgc acatgccgct gctgctgctg catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHQQ QQRHVQLNPE LRFERPDKEY IPPPPLKDMQ DMAKVLFFLS TDKKRYPDGR HRTLDYFRAA VEMFVTEVRQ EYQRQYQQAQ RSGRAMQRLT WKNSGELAIC FACCCDNVKL LYDNLQPGPL KPLWDAFVGQ LAPMLIVQSR VPEAMLSSQT YHIKYMDWVK GGTRYGGEQS AANVRFPSAL DRRVKVETYL RSGAGYKIEA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcagcagc agcagcggca tgtgcagctg aatcccgaat tgcgctttga gaggccagac aaggagtaca tcccaccacc gcccctcaag gacatgcagg acatggctaa ggtgctgttt ttcttgagta ccgataagaa gcgctatccg gatgggcgcc accgcacgct ggattacttc cgtgccgcgg tggagatgtt cgtgacggag gtacggcagg agtaccaacg gcagtatcaa caggcgcagc gcagcggccg tgcgatgcaa cgcttaacat ggaagaactc gggggaactg gcgatttgct ttgcctgctg ctgcgacaat gtgaagcttc tgtacgacaa cctccagccc ggccccctca aaccgctctg ggatgccttt gtgggccagc ttgcgccgat gctcatcgtt caaagtcgag tgccagaggc gatgctgtcg tcccagacgt atcacatcaa gtacatggac tgggttaagg gcggcacacg ttacggtggc gagcagtcgg ccgcgaatgt ccgttttccc tcagcattgg accgccgcgt aaaggtcgaa acgtacctac ggagcggcgc agggtacaag atcgaggctt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for LebrA.19501.a.B4.PW38193
PURIFICATION DATe: 2/28/2017
CONCENTRATION: 38.5mg/ml
OBSERVED MW: 26kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ QQQRHVQLNP ELRFERPDKE YIPPPPLKDM QDMAKVLFFL STDKKRYPDG RHRTLDYFRA AVEMFVTEVR QEYQRQYQQA QRSGRAMQRL TWKNSGELAI CFACCCDNVK LLYDNLQPGP LKPLWDAFVG QLAPMLIVQS RVPEAMLSSQ TYHIKYMDWV KGGTRYGGEQ SAANVRFPSA LDRRVKVETY LRSGAGYKIE A
Validated NT Sequence
atcttactca cttaagcctc gatcttgtac cctgcgccgc tccgtaggta cgtttcgacc tttacacggc ggtccaatgc tgagggaaaa cggacattcg cggccgactg ctcgccaccg taacgtgtgc cgcccttaac ccagtccatg tacttgatgt gatacgtctg ggacgacagc atcgcctctg gcactcgact ttgaacgatg agcatcggcg caagctggcc gacaaaggca tcccagagag gtttgagggg gccgggctgg aggttgtcgt acagcagctt cacattgtcg cagcagcagg caaagcaaat cgccagttcc cccgagttct tccatgttaa gcgttgcatc gcacgaccgc tgcgctgcgc ctgttgatac tgccgttggt actcctgccg tacctccgtc acaaacatct ccaccgcggc acggaagtaa tccagcgtgc ggtggcgccc atccggatag cgcttcttat cggtactcaa gaaaaacagc accttagcca tgtcctgcat gtccttgagg ggcggtggtg ggatgtactc cttgtctggc ctctcaaagc gcaattcggg attcagctgc acatgccgct gctgctgctg catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHQQ QQRHVQLNPE LRFERPDKEY IPPPPLKDMQ DMAKVLFFLS TDKKRYPDGR HRTLDYFRAA VEMFVTEVRQ EYQRQYQQAQ RSGRAMQRLT WKNSGELAIC FACCCDNVKL LYDNLQPGPL KPLWDAFVGQ LAPMLIVQSR VPEAMLSSQT YHIKYMDWVK GGTRYGGEQS AANVRFPSAL DRRVKVETYL RSGAGYKIEA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCAGCAGC AGCAGCGGCA TGTGCAGCTG AATCCCGAAT TGCGCTTTGA GAGGCCAGAC AAGGAGTACA TCCCACCACC GCCCCTCAAG GACATGCAGG ACATGGCTAA GGTGCTGTTT TTCTTGAGTA CCGATAAGAA GCGCTATCCG GATGGGCGCC ACCGCACGCT GGATTACTTC CGTGCCGCGG TGGAGATGTT CGTGACGGAG GTACGGCAGG AGTACCAACG GCAGTATCAA CAGGCGCAGC GCAGCGGCCG TGCGATGCAA CGCTTAACAT GGAAGAACTC GGGGGAACTG GCGATTTGCT TTGCCTGCTG CTGCGACAAT GTGAAGCTTC TGTACGACAA CCTCCAGCCC GGCCCCCTCA AACCGCTCTG GGATGCCTTT GTGGGCCAGC TTGCGCCGAT GCTCATCGTT CAAAGTCGAG TGCCAGAGGC GATGCTGTCG TCCCAGACGT ATCACATCAA GTACATGGAC TGGGTTAAGG GCGGCACACG TTACGGTGGC GAGCAGTCGG CCGCGAATGT CCGTTTTCCC TCAGCATTGG ACCGCCGCGT AAAGGTCGAA ACGTACCTAC GGAGCGGCGC AGGGTACAAG ATCGAGGCTT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT