CENTER ID: | LebrA.19501.a |
ORGANISM: | Leishmania braziliensis MHOM/BR/75/M2904 |
ASSOCIATED DISEASE: | |
CURRENT STATUS: | purified |
COMMUNITY REQUEST: | True |
NIH RISK GROUP: | 2 |
SELECT AGENT: | False |
NIH PRIORITY pathogens category: |
unclassified |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
LebrA.19501.a.B1.GE40857 | full length | 1 | 673 | ||
LebrA.19501.a.B2.GE40680 | C-term domain | 395 | 667 | ||
LebrA.19501.a.B3.GE40681 | J-binding domain | 140 | 288 | ||
LebrA.19501.a.B4.GE41025 | N-extended J-binding domain | 94 | 295 |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
LebrA.19501.a.B4.PW38193 | N-extended J-binding domain | 94 | 295 |
METHOD | RESULTS |
---|---|
comparative modelling | Robetta_79394 |
RESOURCE | REFERENCE ID |
---|---|
EuPathDB: | TriTrypDB:LbrM.35.0480 |
UniProt: | A4HNP7 |
HARVESTED ON: | 12/5/2016 |
SEQUENCED ON: | 12/6/2016 |
EXPECTED MW: | 75kDa |
OBSERVED MW: | 75kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Many (50-100) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | No Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 98 |
PERCENT COVERAGE: | 50 |
HARVESTED ON: | 11/8/2016 |
SEQUENCED ON: | 11/8/2016 |
EXPECTED MW: | 33kDa |
OBSERVED MW: | 32kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | No Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 98 |
PERCENT COVERAGE: | 100 |
HARVESTED ON: | 11/8/2016 |
SEQUENCED ON: | 11/8/2016 |
EXPECTED MW: | 18kDa |
OBSERVED MW: | 18kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Many (50-100) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | No Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 99 |
PERCENT COVERAGE: | 100 |
HARVESTED ON: | 1/4/2017 |
SEQUENCED ON: | 1/5/2017 |
EXPECTED MW: | 25kDa |
OBSERVED MW: | 25kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Many (50-100) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | Moderate Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |
PURIFICATION DATe: | 2/28/2017 |
CONCENTRATION: | 38.5mg/ml |
OBSERVED MW: | 26kDa |
EXPRESSION LEVEL: | Low Expression |
PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
VIAL COUNT (approx.): | 8 |
VIAL VOLUME: | 200µl |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |